Heilongjiang Provincial Environmental Monitoring Central Station

Harbin, China

Heilongjiang Provincial Environmental Monitoring Central Station

Harbin, China
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Yang L.,Harbin Institute of Technology | Chen J.-H.,Heilongjiang Provincial Environmental Monitoring Central Station | Zhang H.,Harbin Institute of Technology | Qiu W.,Harbin Institute of Technology | And 4 more authors.
Food Chemistry | Year: 2012

To elucidate whether the alkali treatment, which is a common process for rice protein extraction, is responsible for the regulation of digestibility and bile acid binding of rice protein, the effects of different degree of alkali treatment (from 0.1% to 0.3% of NaOH), as well as different ratio of arginine (Arg) to lysine (Lys) by 3.0, 3.5, 4.0, 4.5 and 5.0, which was induced by the addition of Arg to rice protein, on the in vitro digestibility and the bile acid binding activity were investigated. The present study clearly demonstrates the alkali treatment plays a major role in controlling the digestibility and the bile acid binding of rice protein through the modification of amino acid composition. Results indicate that the digestibility and the bile acid binding activity of rice protein are closely varying with the ratio of Arg to Lys. This shows the inhibition of digestibility and stimulation of bile acid binding are enhanced by the increase Arg/Lys ratio through the addition of Arg to rice protein. The present study suggests the Arg/Lys ratio controlled by the concentration of Arg and the alkali treatment may be the main modulator responsible for the physiological function of rice protein involved in cholesterol metabolism. © 2011 Elsevier Ltd. All rights reserved.


Li H.,Harbin Institute of Technology | Yang L.,Harbin Institute of Technology | Yang H.-K.,Heilongjiang Provincial Environmental Monitoring Central Station | Sun S.-H.,Rice Research Institute of Heilongjiang Academy of Agricultural Science | And 4 more authors.
Food Bioscience | Year: 2014

To elucidate whether rice protein possess a vital function in regulating high density lipoprotein (HDL) metabolism, the effects of rice protein (RP) on HDL metabolism-related gene expressions and enzyme activities were investigated in adult rats. Compared with casein, lipid contents in plasma and liver were effectively reduced by RP-feeding, accompanying significant increased ratio of HDL to cholesterol or triglyceride. RP increased mRNA levels of lecithin-cholesterol acyltransferase, adenosine triphosphate-binding cassette transporter A1, scavenger receptor class B type I and liver X receptor α, as compared to casein. Hepatic activities of lipoprotein lipase and hepatic lipase were significantly stimulated by RP, whereas no significant difference of plasma lecithin-cholesterol acyltransferase activity was found in RP with respect to casein. Results demonstrated that rice protein could effectively regulate HDL metabolism through modifying the HDL metabolism-related gene expression levels and lipolytic enzyme activities, which involved with a hypocholesterolemic action during the adult period. © 2014 Elsevier Ltd. All rights reserved.


Yang L.,Harbin Institute of Technology | Chen J.,Heilongjiang Provincial Environmental Monitoring Central Station | Xu T.,Heilongjiang Provincial Hospital | Qiu W.,Harbin Institute of Technology | And 4 more authors.
International Journal of Molecular Sciences | Year: 2011

To elucidate whether the digestibility is responsible for the hypocholesterolemic action of rice protein, the effects of rice proteins extracted by alkali (RP-A) and α-amylase (RP-E) on cholesterol metabolism were investigated in 7-week-old male Wistar rats fed cholesterol-free diets for 3 weeks. The in vitro and in vivo digestibility was significantly reduced by RP-A and RP-E as compared to casein (CAS). The digestibility was lower in RP-E than that of RP-A. Compared with CAS, the significant cholesterol-lowering effects were observed in rats fed by RP-A and RP-E. Fecal excretion of bile acids was significantly stimulated by RP-E, but not by RP-A. The apparent cholesterol absorption was more effectively inhibited by RP-E than RP-A because more fecal neutral sterols were excreted in rats fed RP-E. There was a significant correlation between protein digestibility and cholesterol absorption (r = 0.8662, P < 0.01), resulting in a significant correlation between protein digestibility and plasma cholesterol level (r = 0.7357, P < 0.01) in this study. The present study demonstrates that the digestibility of rice protein affected by extraction method plays a major role in the modulation of cholesterol metabolism. Results suggest that the hypocholesterolemic action induced by rice protein with lower digestibility primarily contribute to the inhibition of cholesterol absorption. © 2011 by the authors; licensee MDPI, Basel, Switzerland.


Yang L.,Harbin Institute of Technology | Chen J.-H.,Heilongjiang Provincial Environmental Monitoring Central Station | Xu T.,Heilongjiang Provincial Hospital | Zhou A.-S.,Heilongjiang Provincial Environmental Monitoring Central Station | Yang H.-K.,Heilongjiang Provincial Environmental Monitoring Central Station
Life Sciences | Year: 2012

Aims: To evaluate the effects of rice protein (RP) on glutathione metabolism and oxidative damage. Main methods: Seven-week-old male Wistar rats were fed diets containing casein and RP without cholesterol for 3 weeks. Plasma and liver lipid levels, hepatic accumulation of total glutathione (T-GSH), oxidized glutathione (GSSG), reduced glutathione (GSH), malondialdehyde (MDA) and protein carbonyl (PCO) were measured. In the liver, the total antioxidative capacity (T-AOC), mRNA levels of glutamate cysteine ligase catalytic subunit (GCLC) and glutamate cysteine ligase modulatory subunit (GCLM), and the activities of hepatic catalase (CAT), total superoxide dismutase (T-SOD), γ-glutamylcysteine synthetase (γ-GCS), glutathione S-transferase (GST), glutathione reductase (GR) and glutathione peroxidase (GSHPx) were also measured. Key findings: T-AOC, GCLC and GCLM mRNA levels, antioxidative enzyme activities (T-SOD and CAT) and glutathione metabolism related enzyme activities (γ-GCS, GST, GR and GSHPx) were effectively stimulated by RP feeding compared to casein, and RP significantly reduced the hepatic accumulation of MDA and PCO in rats. These results indicate that lipid-lowering activity was induced by RP feeding. Significance: The present study demonstrates that RP improves oxidative stress primarily through enzymatic and non-enzymatic antioxidative defense mechanisms, reflected by enhancing the antioxidative status and attenuating the oxidative damage to lipids and proteins. These results suggest that RP can prevent hyperlipidemia in part through modifying glutathione metabolism, and sulfur amino acids may be the main modulator of this antioxidative mechanism. © 2012 Elsevier Inc. All rights reserved.


Yang L.,Harbin Institute of Technology | Chen J.-H.,Heilongjiang Provincial Environmental Monitoring Central Station | Xu T.,Heilongjiang Provincial Hospital | Nie M.-H.,Heilongjiang Provincial Hospital | Yang H.-K.,Heilongjiang Provincial Environmental Monitoring Central Station
Gene | Year: 2013

Aging is one of major risk factors for developing hypercholesterolemia. To elucidate the cholesterol-lowering mechanism exerted by rice protein (RP), the effects on hepatic cholesterol outputs and cholesterol metabolism related enzymes were investigated in adult rats, which were fed by casein (CAS) and RP without cholesterol in diets. After 2. weeks of feeding, the significant cholesterol-lowering effect was observed in adult rats fed by RP compared to CAS. The hepatic total- and VLDL-cholesterol secretions into circulation were significantly depressed in RP group, whereas biliary outputs of bile acids and cholesterol were effectively stimulated by RP-feeding, causing an increase in fecal sterol excretion compared to CAS. As a result, the apparent cholesterol absorption was significantly inhibited by RP. RP-feeding significantly increased the activity and gene expression of cholesterol 7α-hydroxylase, whereas acyl-CoA:cholesterol acyltransferase-2 activity and gene expression were significantly decreased by RP as compared with CAS. Neither activity nor gene expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase of RP did differ from CAS in the liver. The present study demonstrates that rice protein can prevent hypercholesterolemia through modifying hepatic cholesterol metabolism under cholesterol-free dietary condition. The findings suggest that hypocholesterolemic action induced by rice protein is attributed in part to the inhibition of cholesterol absorption during the adult period. © 2012 Elsevier B.V.

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