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Song Q.-X.,CAS Institute of Genetics and Developmental Biology | Song Q.-X.,University of Chinese Academy of Sciences | Li Q.-T.,University of Chinese Academy of Sciences | Liu Y.-F.,CAS Institute of Genetics and Developmental Biology | And 8 more authors.
Journal of Experimental Botany | Year: 2013

Soybean is one of most important oil crops and a significant increase in lipid content in soybean seeds would facilitate vegetable oil production in the world. Although the pathways for lipid biosynthesis in higher plants have been uncovered, our understanding of regulatory mechanism controlling lipid accumulation is still limited. In this study, we identified 87 transcription factor genes with a higher abundance at the stage of lipid accumulation in soybean seeds. One of these genes, GmbZIP123, was selected to further study its function in regulation of lipid accumulation. Overexpression of GmbZIP123 enhanced lipid content in the seeds of transgenic Arabidopsis thaliana plants. The GmbZIP123 transgene promoted expression of two sucrose transporter genes (SUC1 and SUC5) and three cell-wall invertase genes (cwINV1, cwINV3, and cwINV6) by binding directly to the promoters of these genes. Consistently, the cell-wall invertase activity and sugar translocation were all enhanced in siliques of GmbZIP123 transgenic plants. Higher levels of glucose, fructose, and sucrose were also found in seeds of GmbZIP123 transgenic plants. These results suggest that GmbZIP123 may participate in regulation of lipid accumulation in soybean seeds by controlling sugar transport into seeds from photoautotrophic tissues. This study provides novel insights into the regulatory mechanism for lipid accumulation in seeds and may facilitate improvements in oil production in soybean and other oil crops through genetic manipulation of the GmbZIP123 gene. © The Author 2013. Source


Liu Y.-F.,CAS Institute of Genetics and Developmental Biology | Li Q.-T.,CAS Institute of Genetics and Developmental Biology | Lu X.,CAS Institute of Genetics and Developmental Biology | Song Q.-X.,CAS Institute of Genetics and Developmental Biology | And 9 more authors.
BMC Plant Biology | Year: 2014

Background: Soybean is one of the most important oil crops. The regulatory genes involved in oil accumulation are largely unclear. We initiated studies to identify genes that regulate this process. Results: One MYB-type gene GmMYB73 was found to display differential expression in soybean seeds of different developing stages by microarray analysis and was further investigated for its functions in lipid accumulation. GmMYB73 is a small protein with single MYB repeat and has similarity to CPC-like MYB proteins from Arabidopsis. GmMYB73 interacted with GL3 and EGL3, and then suppressed GL2, a negative regulator of oil accumulation. GmMYB73 overexpression enhanced lipid contents in both seeds and leaves of transgenic Arabidopsis plants. Seed length and thousand-seed weight were also promoted. GmMYB73 introduction into the Arabidopsis try cpc double mutant rescued the total lipids, seed size and thousand-seed weight. GmMYB73 also elevated lipid levels in seeds and leaves of transgenic Lotus, and in transgenic hairy roots of soybean plants. GmMYB73 promoted PLDα1 expression, whose promoter can be bound and inhibited by GL2. PLDα1 mutation reduced triacylglycerol levels mildly in seeds but significantly in leaves of Arabidopsis plants. Conclusions: GmMYB73 may reduce GL2, and then release GL2-inhibited PLDα1 expression for lipid accumulation. Manipulation of GmMYB73 may potentially improve oil production in legume crop plants. © 2014 Liu et al.; licensee BioMed Central Ltd. Source


Guo Y.-L.,Heilongjiang Provincial Academy of Agricultural science | Song W.-F.,Heilongjiang Provincial Academy of Agricultural science | Li M.,Heilongjiang Provincial Academy of Agricultural science
Chinese Journal of Ecology | Year: 2014

In this study, fomesafen residue and its leaching dynamics in three types of soil were clarified by high performance liquid chromatography (HPLC). When fomesafen was applied with the active ingredient at 1350 g·hm-2, the half-lives of degradation in the meadow, albic and black soils were 25. 7-26. 7, 16. 9-19. 3, and 26. 7-30. 1 days, respectively, which were consistent with the first-order reaction kinetics model. Fomesafen leached gradually with its degradation in soil during 120 days, while most of it retained in the surface soil. In the albic soil, fomesafen leached to 30 cm after 30 days of application and the concentration of fomesafen in 10-30 cm soil reached the maximum after 60 days. In the meadow and black soils, the leaching depths were up to 30 cm after 5 days, and the concentrations of fomesafen in 10-30 cm soil reached the maximum after 14 days. These results indicate that fomesafen is hard to degrade in a soil with a high organic matter content, and the soil physical and chemical properties are main factors influencing fomesafen leaching. The residue toxicity of fomesafen on following rotation crops and its potential pollution to groundwater in the meadow soil and black soils were heavier than in the albic soil. Source


Lu X.,CAS Institute of Genetics and Developmental Biology | Lu X.,University of Chinese Academy of Sciences | Li Q.-T.,CAS Institute of Genetics and Developmental Biology | Li Q.-T.,University of Chinese Academy of Sciences | And 11 more authors.
Plant Journal | Year: 2016

Cultivated soybean has undergone many transformations during domestication. In this paper we report a comprehensive assessment of the evolution of gene co-expression networks based on the analysis of 40 transcriptomes from developing soybean seeds in cultivated and wild soybean accessions. We identified 2680 genes that are differentially expressed during seed maturation and established two cultivar-specific gene co-expression networks. Through analysis of the two networks and integration with quantitative trait locus data we identified two potential key drivers for seed trait formation, GA20OX and NFYA. GA20OX encodes an enzyme in a rate-limiting step of gibberellin biosynthesis, and NFYA encodes a transcription factor. Overexpression of GA20OX and NFYA enhanced seed size/weight and oil content, respectively, in seeds of transgenic plants. The two genes showed significantly higher expression in cultivated than in wild soybean, and the increases in expression were associated with genetic variations in the promoter region of each gene. Moreover, the expression of GA20OX and NFYA in seeds of soybean accessions correlated with seed weight and oil content, respectively. Our study reveals transcriptional adaptation during soybean domestication and may identify a mechanism of selection by expression for seed trait formation, providing strategies for future breeding practice. © 2016 The Authors The Plant Journal © 2016 John Wiley & Sons Ltd Source

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