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Zhou K.,Harbin Medical University | Zhou K.,Daqing Oilfield General Hospital Group | Liang H.,Harbin Medical University | Liang H.,Heilongjiang Province Key Laboratory of Molecular Imaging | And 5 more authors.
Tumor Biology

Carboxypeptidase E (CPE) is one of the most important carboxypeptidases involved in biosynthesis of numerous peptide hormones and neurotransmitters and has an important role in endocrine regulation. A splice variant of CPE (CPE-ΔN) has been detected and the mechanism of CPE-ΔN action in tumorigenesis has been studied in many different cancers. The aim of this study was to examine CPE-ΔN expression in human colorectal cancer (CRC) and to evaluate its possible use as a potential prognostic marker. Two hundred nineteen primary colorectal tumors and corresponding normal tissues were included in the study. We have analyzed CPE-ΔN isoform expression by qRT-PCR and Western blot in 219 CRC patients. Correlations between CPE-ΔN mRNA expression and clinicopathological variables were determined with chi-square tests. Survival probabilities were determined using Kaplan-Meier analysis, and univariate and multivariate analyses of the prognostic factors were performed with a Cox regression model. Our results show that CPE-ΔN is overexpressed in colorectal tumor tissue and that high CPE-ΔN mRNA expression is closely correlated with tumor differentiation, pT classification, pN classification, tumor recurrence, and lymph node metastasis (P = 0.042, 0.036, 0.031, 0.006, and 0.008, respectively). However, no correlation was observed between CPE-ΔN expression and age, gender, tumor localization, gross features, and the tumor size. In addition, patients with high CPE-ΔN expression had a significantly shorter survival (P < 0.001, logrank test). Tumor differentiation, gross feature, pT classification, pN classification, tumor recurrence, lymph node metastasis, and CPE-ΔN status were significantly associated with poor prognosis after performing a univariate Cox survival analysis. High CPE-ΔN expression was also identified as an independent prognostic factor using a multivariate analysis (P = 0.011). Based on these results, we can conclude that CPE-ΔN expression might be a potential prognostic marker for colorectal cancer patients. © 2013 International Society of Oncology and BioMarkers (ISOBM). Source

Li X.,Harbin Medical University | Zhao H.,Central Hospital of Liaoyang City | Wang Q.,Jiamusi University | Liang H.,Harbin Medical University | And 3 more authors.
Molecular Medicine Reports

Diabetic retinopathy (DR) is a common complication of diabetes mellitus (DM) and it is the main cause of loss of vision. In previous years, interest in the biological activities of marine organisms has intensified. The effect of fucoidan from the seaweed Fucus vesiculosus on the molecular mechanisms of numerous diseases has been studied, while to date, its effect on DR was yet to be investigated. Therefore, the aim of the present study was to evaluate the role of fucoidan in DR. The human retinal pigment epithelial cell line ARPE-19 was exposed to high D-glucose in the presence or absence of fucoidan. Cell viability was monitored using MTT and lactate dehydrogenase assays. The intracellular reactive oxygen species (ROS) generation was measured using fluorescence spectrophotometry. Cell apoptosis was measured by flow cytometry using Annexin V-fluorescein isothiocyanate staining. Ca2+ influx was measured with a calcium imaging system and the activation of the extracellular signal-regulated kinase (ERK) protein was evaluated using western blot analysis. The non-toxic fucoidan protected ARPE-19 cells from high glucose-induced cell death and normalized high glucose-induced generation of ROS. Fucoidan also inhibited high glucose-induced cell apoptosis, as well as the Ca2+ influx and ERK1/2 phosphorylation in ARPE-19 cells. Taken together, these findings indicated that fucoidan protects ARPE-19 cells against high glucose-induced oxidative damage via normalization of ROS generation through the Ca2+-dependent ERK signaling pathway. Source

Yang C.,Harbin Medical University | Liang H.,Harbin Medical University | Liang H.,Heilongjiang Province Key Laboratory of Molecular Imaging | Zhao H.,Harbin Childrens Hospital | And 2 more authors.
Experimental and Therapeutic Medicine

Asthma is a disease characterized by chronic airway inflammation, and Th2 cells play a critical role in initiating and sustaining asthmatic inflammation. It has been shown that CD44 expressed on CD4 + T cells plays a critical role in the accumulation of antigen-specific Th2 cells in the development of airway hyperresponsiveness induced by antigen challenge in the airways. The aim of this study was to determine whether there are specific CD44 variant isoforms (CD44v) expressed on lymphocytes from asthmatic patients. We collected whole blood samples from 103 normal subjects, 165 subjects with asthma and 104 with pneumonia. Peripheral blood lymphocyte isolation was performed, and total RNA was extracted from the isolated lymphocytes, using nested PCR for specific CD44v amplification on lymphocytes. Demographic variables were analyzed using linear regression in order to determine whether the expression of CD44v was correlated with these demographic features. The nested PCR results revealed that CD44v5 was expressed by 55.2% of asthma patients, which was significantly higher than levels of expression in the other groups. Lower percentages of individuals in the normal subject group exhibited expression of CD44v5 and CD44v6. The data demonstrated that the percentage of individuals in the pneumonia group expressing CD44v5 was 29.0%, but a higher percentage of these patients expressed CD44v6. CD44v5 expression was positively correlated with IgE levels (p=0.032) in the asthmatic patient group, and CD44v6 was significantly positively correlated with the neutrophil count (p<0.05). CD44v5 was expressed by a higher proportion of asthmatic patients than other subjects and thus may play an important role in the pathogenesis of asthma. These findings may offer a new target for the diagnosis and treatment of asthma and may also provide insights into the mechanisms of asthma development. Source

Liu P.,Harbin Medical University | Liang H.,Harbin Medical University | Liang H.,Heilongjiang Province Key Laboratory of Molecular Imaging | Xue L.,Harbin Medical University | And 5 more authors.
Experimental and Therapeutic Medicine

Despite the improved ability to detect mutations in recent years, tissue specimens cannot always be procured in a clinical setting, particularly from patients with recurrence of tumors or metastasis. Therefore, the aim of this study was to investigate whether plasma is able to be used for mutation analysis instead of tissue specimens. We collected plasma from 62 patients with colorectal cancer (CRC) prior to treatment. DNA extracted from plasma and matched tumor tissues were obtained. Mutations in KRAS were amplified from the tissue specimens and sequenced by regular polymerase chain reaction (PCR) and co-amplification at lower denaturation temperature (COLD)-PCR. Plasma KRAS gene mutation on codon 12 (GGT>GAT) was detected using a nested COLD-PCR/TaqMan®-MGB probe. Mutations in plasma and matched tumors were compared. KRAS mutation on codon 12 (GGT>GAT) was found in 13 (21.0%) plasma specimens and 12 (19.4%) matched tumor tissues. The consistency of KRAS mutations between plasma and tumors was 75% (9/12), which indicated a high correlation between the mutations detected in plasma DNA and the mutations detected in the corresponding tumor DNA (P<0.001; correlation index, k=0.649). Notably, four (6.5%) patients with plasma DNA mutations had no detectable KRAS mutations in the corresponding primary tumors, and three (4.8%) patients with tumor DNA mutations had no detectable KRAS mutations in the corresponding plasma DNA samples. Thus, KRAS mutations in plasma DNA correlate with the mutation status in matched tumor tissues of patients with CRC. Our study provides evidence to suggest that plasma DNA may be used as a potential medium for KRAS mutation analysis in CRC using the COLD-PCR/TaqMan-MGB probe method. Source

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