Heilongjiang Nursing College

Nangang District Harbin, China

Heilongjiang Nursing College

Nangang District Harbin, China
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Gao C.-H.,Heilongjiang University | Zhang L.,Heilongjiang University | Zhang L.,Heilongjiang Nursing College | Hou G.-F.,Heilongjiang University | And 3 more authors.
Inorganic Chemistry Communications | Year: 2017

Two isomorphic chiral lanthanide coordination polymers (CCPs), namely, [Ln2(cpfa)3]n {Ln = Yb Xu et al. (2011) and Lu Zheng et al. (2015) } {H2cpfa = (R)-4-(4-(1-carboxyethoxy)phenoxy)-3-fluorobenzoic acid, have been synthesized under hydro(solvo)thermal conditions. Their structures have been determined by single-crystal X-ray diffraction analyses and further characterized by infrared spectra (IR), powder X-ray diffraction (PXRD), elemental analyses, thermogravimetric (TG) analyses and circular dichroism spectra (CD). Structure analysis reveals that CCPs 1 and 2 are isomorphic, crystallizing in Orthorhombic space group P212121 and giving 3D rhombic framework. Further, CCP 1 show the strong NIR luminescence of Yb(III) ions, suggesting that [cpfa]2 − is able to sensitize the luminescence of lanthanide ions efficiently. The two polymers also exhibit modest SHG efficiency indicating their potential application as optical materials. Thermogravimetric analyses show the remarkable thermal stabilities of the two lanthanide 3D frameworks up to 420 °C. © 2017 Elsevier B.V.


Ma N.,Harbin Medical University | Xiang Y.,Harbin Medical University | Xiang Y.,Yangtze University | Zhang Y.,Harbin Medical University | And 4 more authors.
Journal of Cellular Biochemistry | Year: 2013

Heme oxygenase-1 (HMOX1) is a ubiquitously expressed inducible enzyme that degrades heme to carbon monoxide, biliverdin, and free iron ions. Since 1950, many studies have revealed the role of HMOX1 in reducing the impact of oxidative stress in many types of diseases, such as Alzheimer's disease, heart disease, and the development of tumors. These effects arise as a result of the removal of heme, the biological activities of the products of HMOX1 and the activity of HMOX1 itself. However, HMOX1 has some contradictory effects. The discovery of microRNAs (miRNAs) and their relationship with HMOX1 has provided a new direction for research in this field. Here, we discuss the role of a potential regulatory feedback loop between HMOX1 and miRNAs in pathological processes based on recently published data. We hope to describe a new mechanism for HMOX1 function based on miRNAs to address the contradictory results reported in the literature. © 2013 Wiley Periodicals, Inc.


Zhang C.,Qiqihar Medical University | Sun Y.,Qiqihar Medical University | Sun X.,Qiqihar Medical University | Wu Y.,Qiqihar Medical University | And 3 more authors.
International Journal of Medicinal Mushrooms | Year: 2015

We investigated the anxiolytic effects Agaricus brasiliensis extract (AbSE) on ischemia-induced anxiety using the plus-maze test and the social interaction test. The animals were treated orally with AbSE (4, 8, and 10 mg/kg/d, respectively) for 30 d, followed by middle cerebral artery occlusion–induced cerebral ischemia. Levels of noradrenaline, dopamine, and serotonin in the cerebral cortex of rats, as well as oxidative stress and plasma corticosterone levels were analyzed, respectively. The rota-rod test was carried out to exclude any false positive results in experimental procedures related to anxiety disorders, and the catalepsy test was carried out to investigate whether AbSE induces catalepsy. Our results demonstrate that oral administration of AbSE presented anxiolytic-like effects in the elevated plus-maze test and the social interaction test. Furthermore, AbSE did not induce extrapyramidal symptoms in the catalepsy test. The mechanism underlying the anxiolytic effect of AbSE might be increased brain monoamine levels and plasma corticosterone levels and decreased oxidative stress in cerebral ischemia/reperfusion rats. © 2015 Begell House, Inc.


PubMed | Heilongjiang Nursing College, Harbin Medical University and Qiqihar Medical University
Type: Journal Article | Journal: International journal of medicinal mushrooms | Year: 2015

We investigated the anxiolytic effects Agaricus brasiliensis extract (AbSE) on ischemia-induced anxiety using the plus-maze test and the social interaction test. The animals were treated orally with AbSE (4, 8, and 10 mg/kg/d, respectively) for 30 d, followed by middle cerebral artery occlusion-induced cerebral ischemia. Levels of noradrenaline, dopamine, and serotonin in the cerebral cortex of rats, as well as oxidative stress and plasma corticosterone levels were analyzed, respectively. The rota-rod test was carried out to exclude any false positive results in experimental procedures related to anxiety disorders, and the catalepsy test was carried out to investigate whether AbSE induces catalepsy. Our results demonstrate that oral administration of AbSE presented anxiolytic-like effects in the elevated plus-maze test and the social interaction test. Furthermore, AbSE did not induce extrapyramidal symptoms in the catalepsy test. The mechanism underlying the anxiolytic effect of AbSE might be increased brain monoamine levels and plasma corticosterone levels and decreased oxidative stress in cerebral ischemia/reperfusion rats.


PubMed | Heilongjiang Nursing College, Harbin Medical University and Jiamusi University
Type: Journal Article | Journal: International journal of environmental research and public health | Year: 2015

Using a cross-sectional survey design from 11 public tertiary hospitals (a specialist hospital, four Chinese medicine hospitals, and six general hospitals) in the urban areas of Heilongjiang, we determined the nature of workplace violence that medical staff have encountered in Chinese hospitals and identified factors associated with those experiences of violence. A total of 1129 health workers participated. The specialist hospital had the highest prevalence of physical violence (35.4%), while the general hospitals had the highest prevalence of non-physical violence (76%). Inexperienced medical staff (p < 0.001) were more likely to suffer non-physical violence than physical violence in Chinese medicine hospitals compared to experienced staff. Medical units (p = 0.001) had a high risk of non-physical violence, while surgical units (p = 0.005) had a high risk of physical violence. In general hospitals, staff with higher levels of anxiety about workplace violence were more vulnerable to both physical violence (1.67, 95% CI 1.36-2.10) and non-physical violence (1.309, 95% CI 1.136-1.508) compared to those with lower levels of anxiety, while rotating shift workers had a higher odds of physical violence (2.2, 95% CI 1.21-4.17) and non-physical violence (1.65, 95% CI 1.13-2.41) compared to fixed day shift workers. Thus, prevention should focus not only on high-risk sections of hospitals, but also on the nature of the hospital itself.


Meng F.-Y.,Guangxi Medical University | Ning Y.-L.,Guangxi Medical University | Ning Y.-L.,Beihai Peoples Hospital | Qi J.,Heilongjiang Nursing College | And 6 more authors.
International Journal of Molecular Sciences | Year: 2014

A new water-soluble polysaccharide (longan polysaccharide 1 (LP1)) was extracted and successfully purified from Dimocarpus longan pulp via diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography. The chemical structure was determined using Infrared (IR), gas chromatography (GC) and nuclear magnetic resonance (NMR) analysis. The results indicated that the molecular weight of the sample was 1.1 × 105 Da. Monosaccharide composition analysis revealed that LP1 was composed of Glc, GalA, Ara and Gal in a molar ratio of 5.39:1.04:0.74:0.21. Structural analysis indicated that LP1 consisted of a backbone of →4)-α-D-Glcp-(1→4)-α-D-Galp A-(1→4)-α-D-Glcp -(1→4)-Β-D-Glcp -(1→ units with poly saccharide side chains composed of →2)-Β-D-Fruf-(1→2)-L-sorbose-(1→ attached to the O-6 position of the α-D-Glcp residues. In vitro experiments indicated that LP1 had significantly high antitumor activity against SKOV3 and HO8910 tumor cells, with inhibition percentages of 40% and 50%, respectively. In addition, LP1 significantly stimulated the production of the cytokine interferon-γ (IFN-γ), increased the activity of murine macrophages and enhanced B- and T-lymphocyte proliferation. The results of this study demonstrate that LP1 has potential applications as a natural antitumor agent with immunomodulatory activity. © 2014 by the authors; licensee MDPI, Basel, Switzerland.


Li J.,Jilin University | Yao L.,Harbin Medical University | Li G.,Harbin Medical University | Ma D.,Harbin Medical University | And 4 more authors.
PLoS ONE | Year: 2015

Extrahepatic cholangiocarcinoma (EHCC) is a refractory malignancy with poor prognosis due to its early invasion, metastasis and recurrence after operation. Therefore, understanding the mechanisms of invasion and metastasis is the key to the development of new and effective therapeutic strategies for EHCC. In the present study we demonstrated that miR-221 promoted EHCC invasion and metastasis through targeting PTEN and formed a positive feedback loop with β-catenin/c-Jun signaling pathway. We found miR-221 was upregulated in EHCC specimens and CC cell lines. Moreover, miR-221 was found strongly associated with the metastasis and prognosis of EHCC patients. The expression of PTEN was downregulated in EHCC patients and CC cell lines, and was further demonstrated as one of the downstream targets of miR-221. In addition, our data indicated that β-catenin activated miR-221 through c-jun, while miR-221 enhanced β-catenin signaling induced-epithelial-mesenchymal transition (EMT) by targeting PTEN, hence forming a positive feedback loop in EHCC cell lines. In conclusion, our results suggested that miR-221 promotes EMT through targeting PTEN and forms a positive feedback loop with β-catenin/c-Jun signaling pathway in EHCC. © 2015 Li et al.


PubMed | Heilongjiang Nursing College, Harbin Medical University, Jiamusi University and Qiqihar Medical University
Type: Journal Article | Journal: International journal of environmental research and public health | Year: 2016

Workplace violence in Chinese township hospitals is a major public health problem. We identified the risk factors of healthcare workers worry about experiencing workplace violence in 90 Chinese township hospitals and determined specific measures for differing stages of violence (based on crisis management theory). Participants were 440 general practitioners and 398 general nurses from Heilongjiang Province, China (response rate 84.6%). One hundred and six (12.6%) respondents reported being physically attacked in their workplace in the previous 12 months. Regarding psychological violence, the most common type reported was verbal abuse (46.0%). While most (85.2%) respondents had some degree of worry about suffering violence, 22.1% were worried or very worried. Ordinal regression analysis revealed that being 35 years of age, having a lower educational level, having less work experience, and working night shifts were all associated with worry about workplace violence. Furthermore, those without experience of such violence were more likely to worry about it. Respondents suggested measures for controlling violence included widening channels on medical dispute solutions, improving doctor-patient communication, and advocating for respect for medical workers via the media. Results suggest the target factors for reducing healthcare workers worry by according to the type of education and training and possible measures for limiting workplace violence in township hospitals.


Yao L.,Harbin Medical University | Li F.,Harbin Medical University | Tang Z.,Harbin Medical University | Gao S.,Heilongjiang Nursing College | Wu D.,Harbin Medical University
Asian Pacific Journal of Cancer Prevention | Year: 2012

Aims: Primary hepatocellular carcinoma (HCC) is a common malignancy often related to hepatitis viral infection. Smad4 is known to mediate the TGF-β pathway to suppress tumorigenesis. However, the function of Smad4 in HCC is still controversial. In this study we compared levels of Smad4 in HCC tissues with or without hepatitis virus infection and adjacent normal-appearing liver. Methods: Samples from HCC patients were analyzed for Smad4 protein and mRNA expression by immunohistochemistry (IHC), RT-PCR and Western blotting. Results: We found that tumor tissues expressed less Smad4 mRNA and protein than the adjacent tissues. Most HCC tumor tissues were negative for Smad4 in IHC staining, while the majority of adjacent tissues were positively stained. Interestingly, protein levels were higher in HCC tissues with viral hepatitis than those without virus infection. Suppression of expression appeared closely related to HCC, so that Smad4 appears to function as a tumor suppressor gene (TSG). Conclusion: Patients with hepatitis viral infection, at higher risk for HCC, exhibited increased Smad4 protein expression suggesting hepatitis virus may modulate Smad4 expression, which is functionally distinct from its putative role as a TSG. Smad4 expression may thus be an applicable marker for diagnosis and/or a target to develop therapeutic agents for HCC.


PubMed | Heilongjiang Nursing College and Harbin Medical University
Type: Journal Article | Journal: Cancer investigation | Year: 2016

To identify the prognostic value of c-jun, c-fos, and p53 in oral cancer, we examined the impact of immunohistochemical expression of these markers on tumor progression in 157 oral squamous cell carcinoma (OSCC). We found that c-jun or c-fos was significantly associated with lymph node metastasis, and coexpression of c-jun/c-fos, or c-jun/c-fos/p53 were significantly associated with lymph node metastasis, poor differentiation and clinical stage. The coexpression of c-jun/c-fos/p53 was identified as independent prognostic factors for overall survival. Simultaneous coexpression of these markers in OSCCs might prove to be a useful indicator for differentiation of low and high-risk patients.

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