Heilongjiang Institute of Veterinary Science

Qiqihar, China

Heilongjiang Institute of Veterinary Science

Qiqihar, China
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Gao J.-F.,Heilongjiang Institute of Veterinary Science | Gao J.-F.,Heilongjiang Bayi Agricultural University | Hou M.-R.,Heilongjiang Institute of Veterinary Science | Cui Y.-C.,Heilongjiang Institute of Veterinary Science | And 2 more authors.
Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis | Year: 2017

The complete mitochondrial genome of Drepanidotaenia lanceolata was sequenced. The complete mtDNA sequence is 13 573 bp long and contains 12 protein-coding genes (cox1–3, nad1–6, nad4L, atp6, and cytb), two ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and 2 non-coding regions (NCR). Phylogenetic analysis using three methods (Bayesian inference, maximum parsimony, and maximum likelihood) demonstrates with high statistical support that D. lanceolata is closely related to the genus Hymenolepis. This study reports the first complete mt genome sequence of a representative species of the genus Drepanidotaenia and should provide novel genetic data for further studies of the taxonomy, systematics, and population genetics of these and other related cestodes of socio-economic significance. © 2015 Informa UK Limited, trading as Taylor & Francis Group.


Xiao J.-Y.,Jiamusi University | Gao J.-F.,Heilongjiang Institute of Veterinary Science | Cai L.-S.,Jiamusi University | Dai Y.,Jiamusi University | And 3 more authors.
Mitochondrial DNA | Year: 2013

The present study examined genetic variability among Clonorchis sinensis isolates from four different geographical localities (Guangzhou, Nanning, Jiamusi and Daqing) and host species (cats, dogs, human and rabbits) in Mainland China by sequence analyses of two mitochondrial DNA (mtDNA) genes, namely NADH dehydrogenase subunits 2, 5 (nad2 and nad5) and ribosomal internal transcribed spacer 1 (ITS1). A portion of the ITS1, nad2 (pnad2) and nad5 (pnad5) was amplified by polymerase chain reaction separately from adult C. sinensis individuals and the amplicons were subjected to sequencing from both directions. The length of the sequences of ITS1, pnad2 and pnad5 was 643, 666 and 771 bp, respectively. The intraspecific sequence variations within C. sinensis were 0-1.7% for ITS1, 0-1.4% for pnad2 and 0-0.9% for pnad5. The interspecific sequence variations within other zoonotic trematodes, which were published previously, were 4.5-84.9% for ITS1, 21.9-43.6% for pnad2 and 19.2-48.9% for pnad5. The A+T contents of the sequences were 45.26-45.88% (ITS1), 62.91-63.51% (pnad2) and 58.24-58.63% (pnad5). Phylogenetic analyses using ribosomal and mitochondrial sequence data set, with three different computational algorithms (Bayesian inference, maximum parsimony and maximum likelihood), all revealed distinct groups with high statistical support. These findings demonstrated the existence of low-level intraspecific variations in ribosomal DNA (rDNA) and mtDNA sequences among C. sinensis isolates from four different regions and hosts in China and elucidated that mtDNA sequences and rDNA sequences provided reliable genetic markers for phylogenetic studies of zoonotic trematodes. © 2013 Informa UK, Ltd.


Gao J.-F.,Heilongjiang Bayi Agricultural University | Gao J.-F.,Heilongjiang Institute of Veterinary Science | Zhao Q.,Jilin Agricultural University | Liu G.-H.,Hunan Agricultural University | And 9 more authors.
Gene | Year: 2014

Bunostomum trigonocephalum and Bunostomum phlebotomum are blood-feeding hookworms of sheep and cattle, causing considerable economic losses to the live stock industries. Studying genetic variability within and among hookworm populations is critical to addressing epidemiological and ecological questions. Mitochondrial (mt) DNA is known to provide useful markers for investigations of population genetics of hookworms, but mt genome sequence data are scant. In the present study, the complete mitochondrial DNA (mtDNA) sequences of the sheep and goat hookworm B. trigonocephalum were determined for the first time, and the mt genome of B. phlebotomum from yak in China was also sequenced for comparative analyses of their gene contents and genome organizations. The lengths of mt DNA sequences of B. trigonocephalum sheep isolate, B. trigonocephalum goat isolate and B. phlebotomum China yak isolate were 13,764. bp, 13,771. bp and 13,803. bp in size, respectively. The identity of the mt genomes was 99.7% between B. trigonocephalum sheep isolate and B. trigonocephalum goat isolate. The identity of B. phlebotomum China yak isolate mt genomes was 85.3% with B. trigonocephalum sheep isolate, and 85.2% with B. trigonocephalum goat isolate. All the mt genes of the two hookworms were transcribed in the same direction and gene arrangements were consistent with those of the GA3 type, including 12 protein-coding genes, 2 rRNA genes and 22 tRNA genes, but lacking ATP synthetase subunit 8 gene. The mt genomes of B. trigonocephalum and B. phlebotomum were similar to prefer bases A and T, the contents of A. +. T are 76.5% (sheep isolate), 76.4% (goat isolate) and 76.9% (China yak isolate), respectively. Phylogenetic relationships reconstructed using concatenated amino acid sequences of 12 protein-coding genes with three methods (maximum likelihood, Bayesian inference and neighbor joining) revealed that the B. trigonocephalum and B. phlebotomum represent distinct but closely-related species. These data provide novel and useful genetic markers for studying the systematics, and population genetics of the two ruminant hookworms. © 2014 Elsevier B.V.


Wang C.R.,Jilin Agricultural University | Wang C.R.,Heilongjiang Bayi Agricultural University | Gao J.F.,Heilongjiang Bayi Agricultural University | Gao J.F.,Heilongjiang Institute of Veterinary Science | And 6 more authors.
Journal of Helminthology | Year: 2013

The present study examined sequence variability in four mitochondrial genes, namely cytochrome c oxidase subunit (cox1), cytochrome b (cytb) and NADH dehydrogenase subunits 1 and 5 (nad1 and nad5), among Bunostomum trigonocephalum isolates from four different geographic regions in China. Ten B. trigonocephalum samples were collected from each of the four provinces (Heilongjiang, Jilin, Shaanxi and Yunnan), China. A part of the cox1 (pcox1), cytb (pcytb), nad1 and nad5 genes (pnad1 and pnad5) were amplified separately from individual hookworms by polymerase chain reaction (PCR) and were subjected to direct sequencing in order to define sequence variations and their phylogenetic relationships. The intra-specific sequence variations within B. trigonocephalum were 0-1.9% for pcox1, 0-2.0% for pcytb, 0-1.6% for pnad1 and 0-1.7% for pnad5. The A+T contents of the sequences were 69.6-70.4% (pcox1), 71.9-72.7 (pcytb), 70.4-71.1% (pnad1) and 72.0-72.6% (pnad5). However, the inter-specific sequence differences among members of the family Ancylostomatidae were significantly higher, being 12.1-14.2% for pcox1, 13.7-16.0 for cytb, 17.6-19.4 for nad1 and 16.0-21.6 for nad5. Phylogenetic analyses based on the combined partial sequences of cox1, cytb, nad1 and nad5 using three inference methods, namely Bayesian inference (Bayes), maximum likelihood (ML) and maximum parsimony (MP), revealed that all the B. trigonocephalum samples form monophyletic groups, but samples from the same geographical origin did not always cluster together, suggesting that there was no obvious geographical distinction within B. trigonocephalum based on sequences of the four mtDNA genes. These results demonstrated the existence of low-level intra-specific variation in mitochondrial DNA (mtDNA) sequences among B. trigonocephalum isolates from different geographic regions. © Cambridge University Press 2012.


Lou Y.,Heilongjiang Bayi Agricultural University | Zhang Y.,Heilongjiang Bayi Agricultural University | Qiu J.-H.,Heilongjiang Bayi Agricultural University | Gao J.-F.,Heilongjiang Institute of Veterinary Science | And 4 more authors.
Mitochondrial DNA | Year: 2015

This research aimed at exploring sequence variability in four mitochondrial (mt) genes, namely, cytochrome c oxidase subunit 1 (cox1), cytochrome b (cytb) and NADH dehydrogenase subunits 1 and 5 (nad1 and nad5), among pinworm Aspicularis tetraptera isolates from laboratory mice in four different provinces, China. A part of the cox1 (pcox1), cytb (pcytb), nad1 and nad5 genes (pnad1 and pnad5) were amplified separately from individual pinworms by polymerase chain reaction (PCR) and sequenced to determine sequence variations and examine their phylogenetic relationships. Herein, the intra-specific sequence variations within A. tetraptera were 0-0.5% for pcox1, 0-1.4% for pcytb, 0-1.8% for pnad1 and 0-1.7% for pnad5, respectively. In contrast, the inter-specific sequence differences among members of the Oxyuridae were significantly higher, being 13.7-17.0% for pcox1, 24.5-34.7% for pcytb, 26.6-29.6% for pnad1 and 24.4-25.5% for pnad5, respectively. Three methods, namely, Bayesian inference (BI), maximum likelihood (ML) and maximum parsimony (MP), were used for phylogenetic analyses based on the combined sequences of the four mt gene sequences, and the results indicated that all A. tetraptera samples form monophyletic groups, but samples from the same geographical origin did not always cluster together. This study demonstrated the existence of low-level intra-specific variation in four mtDNA sequences among A. tetraptera isolates from laboratory mice in different geographic regions in China, indicating no obvious geographical distinction among A. tetraptera isolates in China. These findings have important implications for studying systematics, molecular epidemiology and population genetics of A. tetraptera. © 2014 Informa UK Ltd. All rights reserved: reproduction in whole or part not permitted.


Duan H.,Heilongjiang Bayi Agricultural University | Gao J.-F.,Heilongjiang Institute of Veterinary Science | Hou M.-R.,Heilongjiang Institute of Veterinary Science | Zhang Y.,Heilongjiang Bayi Agricultural University | And 7 more authors.
Parasitology International | Year: 2015

The complete mitochondrial (mt) genome sequence of Triodontophorus brevicauda, an intestinal equine nematode parasite was determined for the first time. The circular T. brevicauda mt genome is 14,305. bp in length and contains 36 genes, of which 12 code for protein, 22 for transfer RNA, and two for ribosomal RNA, and lacks atp8 mtDNA gene. Phylogenetic analysis based on the concatenated amino acid sequence of the 12 protein-coding genes was performed using three different tree-building methods. The Strongyloidea cluster divides into two large branches, and each nematode family included in our study forms an independent clade, though paraphyly confounds the issue at some nodes. T. brevicauda clusters together with Cylicocyclus insignis with high statistical support. The mtDNA data in this study not only provide a new mtDNA resource for phylogeny, but also become a novel and useful genetic marker for further studies on the identification, population genetics, and molecular epidemiology of the genus Triodontophorus in equine. © 2015 Elsevier Ireland Ltd.


PubMed | Heilongjiang Bayi Agricultural University and Heilongjiang Institute of Veterinary Science
Type: | Journal: Mitochondrial DNA | Year: 2015

The complete mitochondrial genome of Drepanidotaenia lanceolata was sequenced. The complete mtDNA sequence is 13573bp long and contains 12 protein-coding genes (cox1-3, nad1-6, nad4L, atp6, and cytb), two ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and 2 non-coding regions (NCR). Phylogenetic analysis using three methods (Bayesian inference, maximum parsimony, and maximum likelihood) demonstrates with high statistical support that D. lanceolata is closely related to the genus Hymenolepis. This study reports the first complete mt genome sequence of a representative species of the genus Drepanidotaenia and should provide novel genetic data for further studies of the taxonomy, systematics, and population genetics of these and other related cestodes of socio-economic significance.


PubMed | Heilongjiang Bayi Agricultural University, Heilongjiang Institute of Veterinary Science and Chinese Academy of Agricultural Sciences
Type: | Journal: Parasites & vectors | Year: 2016

Schistosoma turkestanicum is a parasite of considerable veterinary importance as an agent of animal schistosomiasis in many countries, including China. The S. turkestanicum cercariae can also infect humans, causing cercarial dermatitis in many countries and regions of the world. In spite of its significance as a pathogen of animals and humans, there is little transcriptomic and genomic data in the public databases.Herein, we performed the transcriptome Illumina RNA sequencing (RNA-seq) of adult males and females of S. turkestanicum and de novo transcriptome assembly.Approximately 81.1 (female) and 80.5 (male) million high-quality clean reads were obtained and then 29,526 (female) and 41,346 (male) unigenes were assembled. A total of 34,624 unigenes were produced from S. turkestanicum females and males, with an average length of 878 nucleotides (nt) and N50 of 1480 nt. Of these unigenes, 25,158 (72.7 %) were annotated by blast searches against the NCBI non-redundant protein database. Among these, 21,995 (63.5 %), 22,189 (64.1 %) and 13,754 (39.7 %) of the unigenes had significant similarity in the NCBI non-redundant protein (NR), non-redundant nucleotide (NT) and Swiss-Prot databases, respectively. In addition, 3150 unigenes were identified to be expressed specifically in females and 1014 unigenes were identified to be expressed specifically in males. Interestingly, several pathways associated with gonadal development and sex maintenance were found, including the Wnt signaling pathway (103; 2 %) and progesterone-mediated oocyte maturation (77; 1.5 %).The present study characterized and compared the transcriptomes of adult female and male blood fluke, S. turkestanicum. These results will not only serve as valuable resources for future functional genomics studies to understand the molecular aspects of S. turkestanicum, but also will provide essential information for ongoing whole genome sequencing efforts on this pathogenic blood fluke.


PubMed | Heilongjiang Bayi Agricultural University, Heilongjiang Institute of Veterinary Science and Chinese Academy of Agricultural Sciences
Type: Journal Article | Journal: Gene | Year: 2015

The trematode Eurytrema pancreaticum is a parasite of ruminant pancreatic and bile ducts, and also occasionally infects humans, causing eurytremiasis. In spite of it being a common fluke of cattle and sheep in endemic regions, little is known about the genomic resources of the parasite. We sequenced the complete mitochondrial (mt) genome of E. pancreaticum. It is 15,031 bp in size, and encodes 36 genes: 12 protein-coding genes, two ribosomal RNA genes, and 22 transfer RNA genes. The E. pancreaticum mt gene order is the same as that of Dicrocoelium chinensis and Dicrocoelium dendriticum, and all genes are transcribed in the same direction. Phylogenetic analysis based on the concatenated amino acid sequences of 12 protein-coding genes by Bayesian inference shows that E. pancreaticum is closely related to D. chinensis and other members of the family Dicrocoeliidae with strong posterior probability support. The E. pancreaticum mt genome should prove to be a useful resource for comparative mt genomic studies of digenetic trematodes, and will provide a rich source of DNA markers for studies into the systematics, epidemiology, and population genetics of this parasite and other digenean trematodes.


PubMed | Heilongjiang Bayi Agricultural University and Heilongjiang Institute of Veterinary Science
Type: Journal Article | Journal: Parasitology international | Year: 2015

The complete mitochondrial (mt) genome sequence of Triodontophorus brevicauda, an intestinal equine nematode parasite was determined for the first time. The circular T. brevicauda mt genome is 14,305 bp in length and contains 36 genes, of which 12 code for protein, 22 for transfer RNA, and two for ribosomal RNA, and lacks atp8 mtDNA gene. Phylogenetic analysis based on the concatenated amino acid sequence of the 12 protein-coding genes was performed using three different tree-building methods. The Strongyloidea cluster divides into two large branches, and each nematode family included in our study forms an independent clade, though paraphyly confounds the issue at some nodes. T. brevicauda clusters together with Cylicocyclus insignis with high statistical support. The mtDNA data in this study not only provide a new mtDNA resource for phylogeny, but also become a novel and useful genetic marker for further studies on the identification, population genetics, and molecular epidemiology of the genus Triodontophorus in equine.

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