Heilongjiang Higher Education Institutions

Harbin, China

Heilongjiang Higher Education Institutions

Harbin, China

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Shang Q.,Harbin Medical University | Shang Q.,Heilongjiang Key Laboratory of Infection and Immunity | Shang Q.,Heilongjiang Higher Education Institutions | Wang H.,Harbin Medical University | And 12 more authors.
Obesity | Year: 2014

Objective Serological studies on the relationship between adenovirus 36 (Ad36) and an increased risk of obesity development have shown conflicting results. We reviewed the published studies and carried out a meta-analysis to explore this relationship. Methods PubMed was searched until December 2012 for the relative references with sufficient information to estimate odds ratios (ORs) and 95% confidence intervals (CIs). A total of 11 case-control studies, including 2508 obese subjects and 3005 controls, were selected. Results Compared with nonobese controls, Ad36 infection significantly increased the obesity risk by a pooled OR of 1.60 (95% CI = 1.14-2.25; P < 0.01). Meta-regression showed that the types of subject and obesity assessments were potential risk factors. In the subgroup analysis, a significantly increased risk was found in children (OR = 1.95; 95% CI = 1.34-2.85; z = 3.45; P < 0.01) and those with an obesity assessment of BMI ≥ 30 kg/cm2 (OR = 1.89; 95% CI = 1.15-3.10; P < 0.05). Conclusions Ad36 infection is associated with an increased risk of obesity development. To our knowledge, this is the first report to reveal the significant relationship in children with a serological data analysis. Copyright © 2013 The Obesity Society.


Xiao Y.,Harbin Medical University | Ping Y.,Harbin Medical University | Fan H.,Harbin Medical University | Xu C.,Harbin Medical University | And 8 more authors.
Neuro-Oncology | Year: 2013

Background: Accumulating evidence demonstrates that complex diseases may arise from cooperative effects of multiple dysfunctional miRNAs. Thus, identifying abnormal functions cooperatively regulated by multiple miRNAs is useful for understanding the pathogenesis of complex diseases. Methods: In this study, we proposed a multistep method to identify dysfunctional miRNA-mRNA regulatory modules (dMiMRMs) in a specific disease, in which a group of miRNAs cooperatively regulate a group of target genes involved in a specific function. We identified dysfunctional miRNAs, which were differentially expressed and inversely regulated most of their target genes, by integrating paired miRNA and mRNA expression profiles and miRNA target information. Then, we identified cooperative functional units, in each of which a pair of miRNAs cooperatively repressed function-enriched and highly interconnected target genes. Finally, the cooperative functional units were assembled into dMiMRMs. Results: We applied our method to glioblastoma (GBM) and identified GBM-associated dMiMRMs at the population, subtype, and individual levels. We identified 5 common dMiMRMs using all GBM samples, 3 of which were associated with the prognosis in patients with GBM and were better predictors of prognosis than were miRNAs or mRNAs alone. By applying our approach to GBM subtypes, we found consistent dMiMRMs across GBM subtypes, and some subtype-specific dMiMRMs were observed. Furthermore, personalized dMiMRMs were identified, suggesting significant individual differences in different patients with GBM. Conclusions: Our method provides the capability to identify miRNA-mediated dysfunctional mechanisms underlying complex diseases. © 2013 The Author(s).


Shang Q.,Harbin Medical University | Shang Q.,Heilongjiang Higher Education Institutions | Wang Y.,Harbin Medical University | Wang Y.,Heilongjiang Higher Education Institutions | And 12 more authors.
Journal of Clinical Microbiology | Year: 2011

Human papillomavirus type 16 (HPV 16) plays a cardinal role in the pathogenesis of cervical cancer. HPV 16 has intratypic variants which show different geographical distributions and different oncogenic potentials. To analyze the presence of sequence variations of HPV 16 variants in northeast China, 71 cervical carcinomas were identified by HPV typing. HPV 16-positive specimens were analyzed by PCR-directed sequencing in the E6, E7, and L1 genes and the LCR (long control region). The variation data were compared with those of neighboring districts. In this hospital-based study, HPV 16 was the most common type (73.24%). In HPV 16-positive specimens, 67.31% belonged to the European (E) lineage, while 32.69% were Asian (As) variants. The Asian-American (AA), African-1 (Af-1), African-2 (Af-2), and northern American (NA) lineages were not detected. The most frequently observed variation sites were T178G (32.69%) in E6; A647G (34.62%), G666A (38.46%), and T846C (32.69%) in E7; C6826T (36.17%) and G7060A (61.70%) in L1; and G7521A (98.08%) in the LCR. The most prevalent amino acid variations were D25E in E6 and N29S in E7. In addition, 28 novel variations of HPV 16 were reported. Some covariations between different genes were obtained. In this study, HPV 16 variants belonged to the European lineage and the Asian lineage. Compared with neighboring districts, the distribution of HPV 16 variants in northeast China had a typical pattern. As the first report on HPV 16 variants in northeast China, it should be helpful for designing a HPV vaccine and HPV vaccination program in China. Copyright © 2011, American Society for Microbiology. All Rights Reserved.


Wang B.,Harbin Medical University | Qi X.,Harbin Medical University | Li D.,Harbin Medical University | Feng M.,Harbin Medical University | And 3 more authors.
Oncology Letters | Year: 2016

Focal adhesion kinase (FAK) expression has been identified as associated with cancer development and metastasis. Autophosphorylation of FAK at tyrosine (Y) 397 (pY397) performs a critical role in tumor cell signaling. However, few studies have evaluated the expression of pY397 FAK in non-small cell lung cancer (NSCLC). In the present study, pY397 FAK expression in NSCLC was investigated using immunohistochemistry. pY397 FAK staining scores were compared between various groups of specimens and the associations between clinical and pathological characteristics were investigated. A Kaplan-Meier survival curve was used to determine the association between pY397 FAK expression and the prognosis of NSCLC patients. The results of the present study revealed that pY397 FAK expression was localized to the cytoplasm of lung cells, and that pY397 FAK was overexpressed in NSCLC tissues, as well as associated metastatic tissues, when compared with the corresponding non-tumor tissues. However, no significant difference was identified between the pY397 FAK expression in primary lesions and lymph node metastases. Furthermore, pY397 FAK staining scores were not found to be associated with the tumor size, gender, degree of differentiation, histotypes, presence of lymph node metastases or survival rate of NSCLC patients. These results indicate that pY397 FAK is involved with the development of NSCLC, but is not a prognostic marker for the disease. © 2016, Spandidos Publications. All rights reserved.

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