Heilongjiang Entry Exit Inspection and Quarantine Bureau

Harbin, China

Heilongjiang Entry Exit Inspection and Quarantine Bureau

Harbin, China

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Yang X.,Harbin Institute of Technology | Yang X.,Chinese Academy of Agricultural Sciences | Zhang H.,Harbin Institute of Technology | Liu Y.,Heilongjiang Entry Exit Inspection and Quarantine Bureau | And 6 more authors.
Food Chemistry | Year: 2011

A method using solid phase extraction (SPE) cleanup followed by gas chromatography-mass spectrometry (GC-MS) has been established for quantitative determination of 88 pesticide residues in berry fruits including raspberry, strawberry, blueberry and grape. Based on an appraisal of the characteristics of GC-MS, validation experiments were conducted for 88 pesticides. In the method, solid-phase extraction was carried out using Envi-Carb cartridge coupled with NH2-LC cartridge with acetonitrile-toluene (3:1, v/v) as the eluted solvent. In the linear range of each pesticide, the correlation coefficient was R2 ≥ 0.99. At the low, medium and high three fortification levels of 0.05-0.5 mg kg-1, recoveries fell within 63-137%. The relative standard deviation was between 1% and 19% for all 88 pesticides. Low limits of detection (0.006-0.05 mg kg-1) and quantification (0.02-0.15 mg kg-1) were readily achieved with this method for all tested pesticides. © 2011 Elsevier Ltd. All rights reserved.


Cheng Y.,Heilongjiang Entry Exit Inspection and Quarantine Bureau | Zhang M.,Northeast Agricultural University | Hu K.,Heilongjiang Entry Exit Inspection and Quarantine Bureau | Sun F.,Northeast Agricultural University | And 3 more authors.
Food Analytical Methods | Year: 2014

To develop effective alternatives for detecting genetically modified organisms, we constructed one loop-mediated isothermal amplification (LAMP) event-specific detection method for wheat B73-6-1 in this study. This event-specific LAMP assay can be performed within 38 min without any PCR equipment and the LAMP products can be directly observed by the naked eye instead of conventional gel electrophoresis analysis. The limits of detection of these established visual LAMP assays were about six copies of haploid wheat-genomic DNA. Furthermore, the high specificity of LAMP assays was determined. High specificity and sensitivity, cost-effectiveness, and low requirement of equipment of the developed event-specific LAMP assay of B73-6-1 allow this method to be useful in transgenic wheat samples analysis, especially in highly processed products. © 2013 Springer Science+Business Media New York.


Cao J.,Liaoning Entry Exit Inspection and Quarantine Bureau | Xu J.,Liaoning Entry Exit Inspection and Quarantine Bureau | Zhao T.,Liaoning Entry Exit Inspection and Quarantine Bureau | Cao D.,Liaoning Entry Exit Inspection and Quarantine Bureau | And 4 more authors.
Preparative Biochemistry and Biotechnology | Year: 2014

The exogenous fragment sequence and flanking sequence between the exogenous fragment and recombinant chromosome of transgenic wheat B102-1-2 were successfully acquired using genome walking technology. The newly acquired exogenous fragment encoded the full-length sequence of transformed genes with transformed plasmid and corresponding functional genes including ubi, vector pBANF-bar, vector pUbiGUSPlus, vector HSP, reporter vector pUbiGUSPlus, promoter ubiquitin, and coli DH1. A specific polymerase chain reaction (PCR) identification method for transgenic wheat B102-1-2 was established on the basis of designed primers according to flanking sequence. This established specific PCR strategy was validated by using transgenic wheat, transgenic corn, transgenic soybean, transgenic rice, and non-transgenic wheat. A specifically amplified target band was observed only in transgenic wheat B102-1-2. Therefore, this method is characterized by high specificity, high reproducibility, rapid identification, and excellent accuracy for the identification of transgenic wheat B102-1-2. © 2014 Copyright Taylor and Francis Group, LLC.


Xu Y.-G.,Northeast Agricultural University | Liu Z.-M.,Heilongjiang Entry Exit Inspection and Quarantine Bureau | Zhang B.-Q.,Liaoning Entry Exit Inspection and Quarantine Bureau | Qu M.,Harbin University of Commerce | And 3 more authors.
Food Control | Year: 2016

In the present study, a novel target-enriched multiplex PCR (Tem-PCR) assay was developed for simultaneous detection of Salmonella spp., Listeria (L.) monocytogenes, Staphylococcus (S.) aureus, Escherichia (E.) coli O157:H7 and Shigella spp.. DNA primers including universal primer and composite primer pairs were used in this Tem-PCR assay. Of which, the universal primer was linked to the 5'-end of each specific primer designed targeting Salmonella invA gene, Staphylococcus aureus femA gene, Shigella ipaH gene, Listeria monocytogenes hly gene and Escherichia coli O157:H7 rfbE gene, respectively, generating the composite primers. During PCR amplification, the composite primer pairs were employed to enrich target genes of different pathogens in initial cycles and the universal primer was employed to enrich the amplicons produced with the composite primers priming in later PCR cycles. Significantly, the Tem-PCR assay overcomes the amplification disparity resulting from primers competition observed in conventional multiplex PCR assay. With the evaluation of the Tem-PCR assay for the detection of these five foodborne pathogens, the assay showed high specificity to the target bacteria with an analytical detection limit of <2.0 × 102 CFU/mL for each, and practical detection capability, suggesting a novel multiplex PCR method for detecting foodborne pathogens. © 2015 Elsevier Ltd.


Zhang P.,Liaoning Normal University | Xu J.,Liaoning Entry Exit Inspection and Quarantine Bureau | Zheng Q.,Liaoning Entry Exit Inspection and Quarantine Bureau | Luan F.,Heilongjiang Entry Exit Inspection and Quarantine Bureau | And 2 more authors.
Applied Biochemistry and Biotechnology | Year: 2013

Exogenous fragment sequence and flanking sequence between exogenous fragment and recombinant chromosome of transgenic wheat B72-8-11b were successfully acquired through PCR amplification with cross-matched primers from exogenous genes. Newly acquired exogenous fragment covered the full-length sequence of transformed genes such as transformed plasmid and corresponding functional genes including marker uidA, promoter ubiquitin, lacZ, 1Dx5, and part of sequence of the wheat genome. A specific PCR detection method for transgenic wheat B72-8-11b strain was established on the basis of primers designed according to flanking sequence. The designed primers revealed specific amplification of 132 bp product of transgenic wheat B72-8-11b strain. This method is characteristics of high specificity, high reproducibility, rapid identification, and excellent accuracy for the identification of transgenic wheat B72-8-11b strain. © Springer Science+Business Media New York 2013.


Qu H.,Harbin Dege Biotechnology Co. | Zhang W.,Harbin Dege Biotechnology Co. | Zhang X.,Harbin Dege Biotechnology Co. | Wang X.,Heilongjiang Academy of Agricultural science | Li S.,Heilongjiang Entry Exit Inspection and Quarantine Bureau
Biological Chemistry | Year: 2014

Existent nucleic acid isothermal detection techniques for clinical diseases are difficult to promote greatly due to limitations in such aspects as methodology, costs of detection, amplification efficiency and conditions for operation. There is therefore an urgent need for a new isothermal amplification method with the characteristics of high accuracy, easy operation, short time of detection and low costs. We have devised a new method of nucleic acid isothermal amplification using Bst DNA polymerase under isothermal conditions (60-65°C). We call this method of amplification by shortening the distance between forward and reverse primers for nucleic acid isothermal amplification SDAMP. The results demonstrated that this technique is highly sensitive, specific and has short reaction times (40-60 min). Results of sequencing show that the products of SDAMP amplification are mainly polymers formed by series connection of monomers formed through linkage of forward primer and complementary sequences in reverse primer via a few bases. The method is different from current methods of nucleic acid amplification. Our study shows, however, that it is a specific method of nucleic acid isothermal amplification depending on interactions between primers and DNA template. © 2014 by Walter de Gruyter Berlin/Boston.


PubMed | Heilongjiang Entry Exit Inspection and Quarantine Bureau and Northeast Forestry University
Type: Journal Article | Journal: Journal of agricultural and food chemistry | Year: 2015

In the present work, the salidroside metabolite profile in rat urine was investigated, and subsequently the metabolic pathways of salidroside were proposed. After administrations of salidroside at an oral dose of 100 or 500 mg/kg, rat urine samples were collected and pretreated with methanol to precipitate the proteins. The pretreated samples were analyzed by an Acquity ultraperformance liquid chromatography (UPLC) coupled with an HSS T3 column and detected by quadrupole time-of-flight mass spectrometry (Q-TOF-MS) or high-performance liquid chromatography coupled with hybrid triple-quadrupole linear ion trap mass spectrometry (HPLC/Q-trap-MS). A total of eight metabolites were detected and identified on the basis of the characteristics of their protonated ions in the urine samples. The results elucidated that salidroside was metabolized via glucuronidation, sulfation, deglycosylation, hydroxylation, methylation, and dehydroxylation pathways in vivo.


Zhang M.,Northeast Agricultural University | Huo N.,Northeast Agricultural University | Liu Y.,Northeast Agricultural University | Qiu Y.,Northeast Agricultural University | And 4 more authors.
European Food Research and Technology | Year: 2012

In this study, 3′-flanking sequence between the host plant DNA and the integrated gene construct of pHMW1Dx5 vector in transgenic wheat B73-6-1 was revealed by means of adaptor PCR; thus, the fragment with the length of 3. 1 kb was obtained, including a 190-bp wheat genomic DNA, which demonstrates that this HMW-GS gene was located on the wheat chromosome 3B. And the event-specific PCR primers were designed based upon the revealed 3′-flanking sequence; the conventional qualitative PCR and quantitative SYBR real-time PCR detection methods employing these primers were successfully developed. In conventional qualitative PCR assay, the limit of detection was 0. 1 % for B73-6-1 wheat genomic DNA for one reaction. In the quantitative SYBR real-time PCR assay, the limit of detection and limit of quantification were 10 and 100 haploid genome copies, respectively. In addition, three mixed blind wheat samples with known B73-6-1 contents were detected using the established real-time PCR systems, and the ideal results indicated that the established event-specific real-time PCR detection systems were reliable, sensitive and accurate. © 2012 Springer-Verlag Berlin Heidelberg.


Sheng P.-F.,Northeast Agricultural University | Sheng P.-F.,Liaoning Medical University | Jiang Y.,Northeast Agricultural University | Jiang Y.,Liaoning Medical University | And 5 more authors.
BioMetals | Year: 2014

Selenium (Se) plays an important role in the brain development, function, and degeneration, nutritional encephalomalacia is closely related with dietary Se in avian. However, there is little evidence on the relationship between inflammation and encephalomalacia in avian and the mechanism which Se regulates the inflammatory response in brain tissues remains to be unclear. The present paper describes the effects of Se-deficient granulated diet on one transcription factor-nuclear factor kappaB and four pro-inflammatory cytokines-tumor necrosis factor, cyclooxygenase2, inducible nitric oxide synthase and Prostaglandin E synthase mRNA expression in the chicken brain tissues associated encephalomalacia. One hundred male chickens (1 day old; Weiwei Co. Ltd., Harbin, China) were divided into two groups (50 chickens per group). The expression levels in the brain tissues (cerebral gray matter, cerebral white matter, marrowbrain, cerebellum, thalamus and brain stem) were determined by real-time PCR on days 15, 25, 35, 45, and 55, respectively. The results showed the productions of pro-inflammatory mediators were increased following Se-deficiency. These data indicate the correlations between nutritional encephalomalacia and inflammatory response and the activity of inflammatory response in chicken brain may be induced by Se-deficiency. © 2013 Springer Science+Business Media New York.


Zhang M.,Northeast Agricultural University | Yu Y.,Northeast Agricultural University | Gao X.,Northeast Agricultural University | Zhang K.,Northeast Agricultural University | And 3 more authors.
European Food Research and Technology | Year: 2015

Various PCR technologies have been developed for the execution of genetically modified organism (GMO) labeling policies of which an event-specific PCR detection method based on the flanking sequence of exogenous inserted DNA is the key trend in GMO detection due to its high specificity. In this study, 3′ flanking sequence between the host wheat DNA and the exogenous integrated gene construct of pHMW1Dx5 vector in transgenic wheat B72-8-11 was revealed by means of adaptor PCR, thus the fragment with the length of 210 bp was obtained, including a 144 bp unknown wheat genome DNA sequence. The event-specific quantitative PCR primers were designed based upon the revealed 3′ flanking sequence, and a SYBR Green I real-time PCR assay was subsequently applied. In the quantitative SYBR Green I real-time PCR assay, the LOD and LOQ were 10 and 20 haploid genome copies, respectively. In addition, three mixed wheat B72-8-11 samples with known contents were detected using this established quantitative PCR system, and the ideal results indicated that the developed event-specific quantitative PCR detection method could be used for identification and quantification of B72-8-11 wheat and its derivates. © 2014, Springer-Verlag Berlin Heidelberg.

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