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Zhang M.,Northeast Agricultural University | Huo N.,Northeast Agricultural University | Liu Y.,Northeast Agricultural University | Qiu Y.,Northeast Agricultural University | And 4 more authors.
European Food Research and Technology | Year: 2012

In this study, 3′-flanking sequence between the host plant DNA and the integrated gene construct of pHMW1Dx5 vector in transgenic wheat B73-6-1 was revealed by means of adaptor PCR; thus, the fragment with the length of 3. 1 kb was obtained, including a 190-bp wheat genomic DNA, which demonstrates that this HMW-GS gene was located on the wheat chromosome 3B. And the event-specific PCR primers were designed based upon the revealed 3′-flanking sequence; the conventional qualitative PCR and quantitative SYBR real-time PCR detection methods employing these primers were successfully developed. In conventional qualitative PCR assay, the limit of detection was 0. 1 % for B73-6-1 wheat genomic DNA for one reaction. In the quantitative SYBR real-time PCR assay, the limit of detection and limit of quantification were 10 and 100 haploid genome copies, respectively. In addition, three mixed blind wheat samples with known B73-6-1 contents were detected using the established real-time PCR systems, and the ideal results indicated that the established event-specific real-time PCR detection systems were reliable, sensitive and accurate. © 2012 Springer-Verlag Berlin Heidelberg. Source


Sheng P.-F.,Northeast Agricultural University | Sheng P.-F.,Liaoning Medical University | Jiang Y.,Northeast Agricultural University | Jiang Y.,Liaoning Medical University | And 5 more authors.
BioMetals | Year: 2014

Selenium (Se) plays an important role in the brain development, function, and degeneration, nutritional encephalomalacia is closely related with dietary Se in avian. However, there is little evidence on the relationship between inflammation and encephalomalacia in avian and the mechanism which Se regulates the inflammatory response in brain tissues remains to be unclear. The present paper describes the effects of Se-deficient granulated diet on one transcription factor-nuclear factor kappaB and four pro-inflammatory cytokines-tumor necrosis factor, cyclooxygenase2, inducible nitric oxide synthase and Prostaglandin E synthase mRNA expression in the chicken brain tissues associated encephalomalacia. One hundred male chickens (1 day old; Weiwei Co. Ltd., Harbin, China) were divided into two groups (50 chickens per group). The expression levels in the brain tissues (cerebral gray matter, cerebral white matter, marrowbrain, cerebellum, thalamus and brain stem) were determined by real-time PCR on days 15, 25, 35, 45, and 55, respectively. The results showed the productions of pro-inflammatory mediators were increased following Se-deficiency. These data indicate the correlations between nutritional encephalomalacia and inflammatory response and the activity of inflammatory response in chicken brain may be induced by Se-deficiency. © 2013 Springer Science+Business Media New York. Source


Yang X.,Harbin Institute of Technology | Yang X.,Chinese Academy of Agricultural Sciences | Zhang H.,Harbin Institute of Technology | Liu Y.,Heilongjiang Entry Exit Inspection and Quarantine Bureau | And 6 more authors.
Food Chemistry | Year: 2011

A method using solid phase extraction (SPE) cleanup followed by gas chromatography-mass spectrometry (GC-MS) has been established for quantitative determination of 88 pesticide residues in berry fruits including raspberry, strawberry, blueberry and grape. Based on an appraisal of the characteristics of GC-MS, validation experiments were conducted for 88 pesticides. In the method, solid-phase extraction was carried out using Envi-Carb cartridge coupled with NH2-LC cartridge with acetonitrile-toluene (3:1, v/v) as the eluted solvent. In the linear range of each pesticide, the correlation coefficient was R2 ≥ 0.99. At the low, medium and high three fortification levels of 0.05-0.5 mg kg-1, recoveries fell within 63-137%. The relative standard deviation was between 1% and 19% for all 88 pesticides. Low limits of detection (0.006-0.05 mg kg-1) and quantification (0.02-0.15 mg kg-1) were readily achieved with this method for all tested pesticides. © 2011 Elsevier Ltd. All rights reserved. Source


Zhang M.,Northeast Agricultural University | Yu Y.,Northeast Agricultural University | Gao X.,Northeast Agricultural University | Zhang K.,Northeast Agricultural University | And 3 more authors.
European Food Research and Technology | Year: 2015

Various PCR technologies have been developed for the execution of genetically modified organism (GMO) labeling policies of which an event-specific PCR detection method based on the flanking sequence of exogenous inserted DNA is the key trend in GMO detection due to its high specificity. In this study, 3′ flanking sequence between the host wheat DNA and the exogenous integrated gene construct of pHMW1Dx5 vector in transgenic wheat B72-8-11 was revealed by means of adaptor PCR, thus the fragment with the length of 210 bp was obtained, including a 144 bp unknown wheat genome DNA sequence. The event-specific quantitative PCR primers were designed based upon the revealed 3′ flanking sequence, and a SYBR Green I real-time PCR assay was subsequently applied. In the quantitative SYBR Green I real-time PCR assay, the LOD and LOQ were 10 and 20 haploid genome copies, respectively. In addition, three mixed wheat B72-8-11 samples with known contents were detected using this established quantitative PCR system, and the ideal results indicated that the developed event-specific quantitative PCR detection method could be used for identification and quantification of B72-8-11 wheat and its derivates. © 2014, Springer-Verlag Berlin Heidelberg. Source


Li D.-P.,Harbin Medical University | Du C.,Harbin Medical University | Zhang Z.-M.,Harbin Medical University | Li G.-X.,Harbin Medical University | And 6 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2014

The present systematic review and meta-analysis was conducted to assess any association between breastfeeding and the risk of ovarian cancer. A systematic search of published studies was performed in PUBMED and EMBASE and by reviewing reference lists from retrieved articles through March 2013. Data extraction was conducted independently by two authors. Pooled relative risk ratios were calculated using random-effect models. Totals of 5 cohort studies and 35 case-control studies including 17,139 women with ovarian cancer showed a 30% reduced risk of ovarian cancer when comparing the women who had breastfed with those who had never breastfed (pooled RR = 0.70, 95% CI: 0.64-0.76; p = 0.00), with significant heterogeneity in the studies (p = 0.00; I2 = 76.29%). A significant decreasd in risk of epithelial ovarian cancer was also observed (pooled RR = 0.68, 95% CI: 0.61-0.76). When the participants were restricted to only parous women, there was a slightly attenuated but still significant risk reduction of ovarian cancer (pooled RR = 0.76, 95% CI: 0.69-0.83). For total breastfeeding duration, the pooled RRs in the < 6 months, 6-12 months and > 12 months of breastfeeding subgroups were 0.85 (95% CI: 0.77-0.93), 0.73 (95% CI: 0.65-0.82) and 0.64 (95%CI: 0.56-0.73), respectively. Meta-regression of total breastfeeding duration indicated an increasing linear trend of risk reduction of ovarian cancer with the increasing total breastfeeding duration (p = 0.00). Breastfeeding was inversely associated with the risk of ovarian cancer, especially long-term breastfeeding duration that demonstrated a stronger protective effect. Source

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