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Wang F.,Qiqihar University | Li W.,Heilongjiang University | Li W.,Heilongjiang Animal Husbandry Research Institute | Qu Y.,Heilongjiang University | And 2 more authors.
Journal of Animal and Veterinary Advances | Year: 2014

Proteomics is an important supplement for genomics research, every process of life is controlled by interactions between proteins. Proteomics is an objective, complicated, interlaced and precision controlled response network and is widely used in the study of animal development, physiological ecology and diseases, etc. Systems biology is used to sort and integrate these research results, furthermore explain the phenomenon of life at the protein level. This requires researchers of genetics, chemistry, biology, cell biology, engineering, mathematics, informatics and multi-disciplinary to make efforts collectively. In this study, researchers review the current status of proteomic technologies, discuss the research on proteomics in bovine and the development prospect of proteomics. © Medwell Journals, 2014.

Yang X.,Centers for Disease Control and Prevention | Liu C.,Chinese Academy of Agricultural Sciences | Liu C.,Northeast Agricultural University | Liu F.,Chinese Academy of Agricultural Sciences | And 7 more authors.
Virus Genes | Year: 2014

A total of 158 serum samples and 510 nasal swab specimens were collected between September 2010 and May 2012, from dogs exhibiting respiratory symptoms, in order to investigate the epidemiology of H3N2 canine influenza viruses (CIVs) in the Liaoning province of China. Serological surveillance demonstrated that 10.8 % (17/158) of serum samples were positive for H3N2 canine influenza. Two H3N2 influenza viruses, A/canine/Liaoning/27/2012 and A/canine/Liaoning/H6/2012, were isolated from pet dogs in 2012. Phylogenetic analysis indicated that the genes from these two viruses were closely related to those of avian-origin, H3N2 subtype CIVs from China and Thailand. Genetic analysis of eight genes revealed that these two H3N2 canine influenza isolates were highly similar (99.2–99.8 %) to the current common strains in Asia. Analysis of the genotype demonstrated that each gene of the two strains in this study had the same genotype (K, G, E, 3B, F, 2D, F, 1E) as those prevalent in H3N2 CIVs. Our findings further confirm that avian-origin H3N2 canine influenza has become established in China. Conducting extensive serological and epidemiological surveillance is necessary to develop an effective vaccine against this disease. © 2014, Springer Science+Business Media New York.

Li W.,Heilongjiang Bayi Agricultural University | Li W.,Heilongjiang Animal Husbandry Research Institute | Qu Y.,Heilongjiang Bayi Agricultural University | Bao J.,Northeast Agricultural University | And 6 more authors.
Journal of Animal and Veterinary Advances | Year: 2013

In the world wide, it is very common that calves feed Total Mixed Rations (TMR). In this study, researchers want to evaluate TMR feed plus early weaned whether it is one kind of burden to the calves' digestive function. Thirty nine Holsteinbull calves were randomly assigned to 3 groups: Early-Weaned (EW), Low Quantity Milk (LQM) and High Quantity Milk (HQM) to evaluate the effect of diets on the calves' grow and rumen function. The calves were slaughtered at the 0, 7, 30, 60 and 90 days in each group. The activity of protease among the three groups increased with calves' growth and activity in EW and LQW did not differ was lower than that in HQM. pH of ruminal fluid in HQW was lower than that in EW and LQM at the 30 and 60 days but did not differ at 7 and 90 days. The calves that were fed without solid feed before 60 days had stunted growth of ruminal papillae length (white colour). This study suggests that it could prevent the ruminal function from formation that calves only intake liquid food before the 60 days. Calves feed TMR and early weaned may stimulated the development of rumen digestion function. © Medwell Journals, 2013.

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