Heilongjiang Agricultural economics Vocational College

Mudanjiang, China

Heilongjiang Agricultural economics Vocational College

Mudanjiang, China
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Du P.,Beijing Forestry University | Du P.,Heilongjiang Agricultural economics Vocational College | Cui B.-K.,Beijing Forestry University | Dai Y.-C.,Beijing Forestry University | Dai Y.-C.,CAS Shenyang Institute of Applied Ecology
International Journal of Medicinal Mushrooms | Year: 2011

The genetic diversity of 40 wild Auricularia polytricha strains within ten natural populations in tropical China and five cultivated strains were analyzed by using twelve inter-simple sequence repeat (ISSR) primers. At the species level, the percentage of polymorphic loci (P) = 99.8%, the effective number of alleles (N e) = 1.3569, Nei's gene diversity (H) = 0.2398, Shannon information index (I) = 0.3896, and total genetic diversity (H t) = 0.2346 indicate a high level of genetic diversity in wild A. polytricha. At the population level of the wild fungus, P = 43.51%, N e = 1.2675, H = 0.1702, I = 0.2504. However, the genetic diversity of cultivated strains was the lowest of all populations (N e = 1.1584, H = 0.0940, I = 0.1440). A moderate degree of genetic differentiation (G st = 0.347) among the sampled wild populations was detected based on Nei's gene diversity analysis, suggesting that 65.3% of variation existed within this population. The high genetic variation level within wild populations may mostly result from a low level of gene flow (N m = 0.9408) and random genetic drift. © 2011 Begell House, Inc.


Du P.,Heilongjiang Agricultural economics Vocational College | Cao T.-X.,Heilongjiang Agricultural economics Vocational College | Cui B.-K.,Beijing Forestry University | Yuan Y.,Beijing Forestry University | Dai Y.-C.,Beijing Forestry University
International Journal of Medicinal Mushrooms | Year: 2016

Auricularia species are well known for their traditional edible and medicinal importance. In this study, sequence-related amplified polymorphism (SRAP) markers were used to evaluate the genetic diversity and relationships of 24 Auricularia strains obtained from different ecological regions of China. A total of 402 polymorphic loci were detected using 11 SRAP primer combinations with 100% polymorphic loci. The similarity coefficient ranged from 0.662 to 0.903, with an average of 0.73. Cluster analysis using the pair-group method using arithmetic average, based on the data from SRAP loci amplified by 11 primer pairs, showed that 24 Auricularia strains were distinguished into 4 groups with 73% similarity. The results of principal coordinate analysis were in accordance with pair-group method using arithmetic average clustering, and the first, most informative coordinate accounted for 74.38% of all the variations, which indicated a high level of genetic diversity among Auricularia strains. In general, SRAP markers provide a powerful tool to discriminate Auricularia strains and to evaluate their genetic relationships. © 2016 Begell House, Inc. www.begellhouse.com.


Wu F.,CAS Institute of Microbiology | Yuan Y.,CAS Institute of Microbiology | Malysheva V.F.,RAS Komarov Botanical Institute | Du P.,Heilongjiang Agricultural economics Vocational College | Dai Y.-C.,CAS Institute of Microbiology
Phytotaxa | Year: 2014

Phylogenetic analysis of the Auricularia auricula-judae complex was carried out using ITS and nLSU ribosomal RNA gene regions, and morphology of the A. auricula-judae complex and related species is examined based on 33 wild collections and 10 cultivated samples worldwide. The phylogenetic analysis presented here showed that the wild and cultivated samples previously identified as A. auricula-judae in China are different from those from Europe (the type locality). So far no exist name for the most important Chinese Auricularia species is available, and thus a new species, Auricularia heimuer, is described and illustrated. The new species is characterized by effused-reflexed or substipitate basidiomata with fawn to reddish brown color when fresh and vinaceous gray to dark gray when dry, pilose upper surface usually with a few folds when dry, short abhymenial hairs without branching and measured as 50–150 × 4–6.5 μm, usually presence of medulla, long clavate basidia with oil guttules and measured as 40–67 × 3.0–6.5 μm, and allantoid spores measured as 11–13 × 4–5 μm. A. auricula-judae is not found in China, and most probably has a distribution in Europe only. In addition, A. americana and A. villosula are the first time reported in China. Both A. heimuer and A. villosula grow on angiosperm wood, while A. americana is found on gymnosperm wood exclusively. © 2014 Magnolia Press.


Zhang J.,Heilongjiang Agricultural economics Vocational College | Zhang J.,Northeast Forestry University | Ma L.,Northeast Forestry University | Ding X.H.,Northeast Forestry University | And 3 more authors.
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2014

Soil microbe activity is the most important decomposer in forest ecosystems and is a sensitive indicator of environmental changes. It plays a crucial role in wetland carbon and nitrogen cycles. However, few studies on soil microbial biomass carbon and nitrogen have been conducted in the Zhalong wetland, especially studies of the seasonal dynamics of soil microbial biomass carbon and nitrogen in different habitats. In this study, in order to investigate the soil fertility of different habitats based on the soil microbial biomass, the soil microbial biomass C and N were observed in three soil layers (0-10 cm, 10-20 cm, 20-30 cm) of a reed habitat and meadow habitat in the Zhalong wetland. Meanwhile, the relationships between the soil microbial biomass and soil environmental factors were also analyzed. The experimental design included two habitat types with three 20 m-20 m plots in each habitat type. In each plot, we took random soil samples in three soil layers (0-10 cm, 10-20 cm, and 20-30 cm) from five spots every month from May to September, 2010. The samples in each plot were mixed to produce a combined sample for soil microbial biomass carbon and nitrogen determination. All soil samples were stored at 2 °C before analysis within one week of sampling. The results showed that the microbial biomass C and N for different habitats displayed a vertical distribution in decreasing order of 0-10 cm > 10-20 cm > 20-30 cm. In addition, the microbial biomass C and N in the meadow habitat was greater than that of the reed habitat. During the whole growing season, the microbial biomass C and N in the two habitats showed a "W" shaped variation pattern. The variation profiles of the two habitats decreased with soil depth, but the variation in meadow habitat was significantly larger than that in the reed habitat. However, the correlation analysis showed the soil microbial biomass C in the reed habitat had an extremely significant positive correlation with the soil water content (P < 0.01) and had an extremely significant negative correlation with soil pH value (P < 0.01). The soil microbial biomass N in the reed habitat had an extremely significant positive correlation with soil water content, organic carbon and hydrolysable nitrogen (P < 0. 01) and had an extremely significant negative correlation with soil pH value (P < 0.01). The soil microbial biomass C in the meadow habitat had an extremely significant positive correlation with soil water content, organic carbon, available phosphorus, hydrolysable nitrogen (P < 0.01) and had a significant positive correlation with soil pH value (P < 0.05). The soil microbial biomass N in the meadow habitat had an extremely significant positive correlation with soil water content and available phosphorus (P< 0.01) and had a significant positive correlation with organic carbon (P < 0.05). We concluded that the variations in litterfall amount and composition, and the soil physical and chemical properties among the different habitat types mainly contributed to the differences in the seasonal dynamics of the soil microbial biomass C and N in the Zhalong Wetland.


Du P.,Beijing Forestry University | Du P.,Heilongjiang Agricultural economics Vocational College | Cui B.K.,Beijing Forestry University | Dai Y.C.,Beijing Forestry University
Journal of Medicinal Plants Research | Year: 2011

As an economically and medically important fungus, Auricaularia polytricha has been collected and cultivated widely in China, however, its diversity has not yet investigated previously. The genetic diversity of 20 wild A. polytricha strains from Yunnan Province and four cultivated strains from Henan and Sichuan Province were profiled by using 10 pairs of sequence-related amplified polymorphism (SRAP) primers. A dendrogram tree was constructed by unweighted pair-group method with arithmetic means (UPGMA) method according to the similarity coefficient among strains. A total of 425 (P=99.8%) and 37 (P=40.7%) polymorphic loci were detected in the wild and cultivated strains respectively, and the 24 strains were classified into six groups with the similarity coefficient of 0.74. The genetic diversity of the wild strains was much higher than that of the cultivated strains, and SRAP marker was an effective method to evaluate the genetic diversity of A. polytricha. © 2011 Academic Journals.


Du P.,Beijing Forestry University | Du P.,Heilongjiang Agricultural economics Vocational College | Cui B.-K.,Beijing Forestry University | Zhang C.-F.,Heilongjiang Agricultural economics Vocational College | Dai Y.-C.,Beijing Forestry University
Biochemical Systematics and Ecology | Year: 2013

Auricularia auricula-judae is an edible and medicinal jelly mushroom, and it is widely cultivated in China. In the present study, inter-simple sequence repeat (ISSR) molecular marker was employed to assess the genetic diversity of 22 wild strains and two cultivars of A. auricula-judae obtained from many ecological regions of China. With the use of 11 ISSR primers, a total of 368 (99.7%) and 81 (71.7%) polymorphic loci in wild and cultivated strains were detected, respectively. The mean genetic similarity of A. auricula-judae was 0.75, based on the dendrogram generated via similarity coefficient, all the tested strains were classified into six groups at a similarity level of 76%, and the two cultivated strains consisted of one group alone. The results of principal coordinate analysis were in accordance with UPGMA clustering, and the first most informative coordinate accounted for 75.9% of the variations, indicated a high level of genetic diversity among wild A. auricula-judae strains. In general, ISSR marker was an effective method to discriminate the A. auricula-judae stains and to evaluate their genetic diversity. © 2012 Elsevier Ltd.


Du P.,Beijing Forestry University | Du P.,Heilongjiang Agricultural economics Vocational College | Cui B.K.,Beijing Forestry University | Dai Y.C.,Beijing Forestry University | Dai Y.C.,CAS Shenyang Institute of Applied Ecology
Cryptogamie, Mycologie | Year: 2012

The genetic diversity of Auricularia polytricha among 27 wild populations in China was analyzed by using inter-simple sequence repeat (ISSR) markers. At the species level a total of 509 loci were amplified using eleven ISSR primers with the percentage of polymorphic loci (P) = 100%, Nei's gene diversity (H) = 0.1935, Shannon information index (I) = 0.3274, and total genetic diversity (Ht) = 0.1918. At the population level, P = 35.3%, H = 0.1277 and I = 0.1907 whereas diversity within populations (HS) is 0.1135. A high degree of genetic differentiation (Gst = 0.4081) among populations was detected. This genetic structure is likely due to the combined effects of the limited gene flow (Nm = 0.7251), geographical isolation, and random genetic drift. Clustering analysis revealed that a remarkable geographical relatedness exists among strains within population, whereas no distinct geographical pattern occurs in the genetic variation among populations. Furthermore, clustering analysis did not suggest genetic variation to be correlated with the host association. © 2012 Adac. Tous droits réservés.


Liu Z.-Q.,Northeast Forestry University | Ma L.,Northeast Forestry University | Jiao Y.,Northeast Forestry University | Zhang J.,Heilongjiang Agricultural economics Vocational College | And 4 more authors.
Chinese Journal of Applied Ecology | Year: 2015

In order to study the insect community structure of typical artificial and primeval forests during different restoring stages in Xiaoxing'an Mountains, we had systematically investigated the insect communities of four typical forests in Liangshui Natural Reserve. A total of 11712 specimens of 293 species were collected, which belonged to 81 families of 10 orders, with Hymenoptera, Diptera, and Coleoptera being the dominant groups. The community stability analysis on insect communities showed that the insect communities of Dahurian larch plantation and original broadleaved Korean pine forest were more stable than other forest types. The principal components analysis indicated that predatory and neutral insects played important roles in the community stability which increased with the increasing predatory insect species and quantities. Polar ordination analysis showed that there was little difference between the environmental factors of Korean pine plantation and the original Korean pine forest. ©, 2015, Editorial Board of Chinese Journal of Applied Ecology. All right reserved.


Man L.-L.,Northeast Agricultural University | Man L.-L.,Heilongjiang Agricultural economics Vocational College | Meng X.-C.,Northeast Agricultural University | Zhao R.-H.,Northeast Agricultural University | Xiang D.-J.,Heilongjiang Agricultural economics Vocational College
International Dairy Journal | Year: 2014

The function of plNC8HK gene and plnD gene on the bacteriocin production of Lactobacillus plantarum KLDS1.0391 was studied. The results of bioinformatics analysis showed that PlNC8HK protein was a histidine protein kinase and a cytoplasmic membrane protein with six transmembrane helices. PlnD protein was a response regulator of the LytR/AlgR family. Quantitative real-time PCR revealed that plNC8HK and plnD genes of L.plantarum KLDS1.0391 were up-regulated significantly (P<0.01) when L.plantarum KLDS1.0391 co-cultured with Lactobacillus helveticus KLDS1.9207. The plNC8HK- plnD mutant was also constructed. The change of antibacterial activity, AI-2 activity and cell number of plNC8HK- plnD mutant in co-culture and mono-culture demonstrated that plNC8HK and plnD genes were essential for bacteriocin production in L.plantarum KLDS1.0391, and the increase of bacteriocin production correlated closely with plNC8HK and plnD genes in co-culture. These results are beneficial to understand the role of two components system in the bacteriocin production of L.plantarum. © 2013 Elsevier Ltd.


PubMed | Beijing Forestry University and Heilongjiang Agricultural economics Vocational College
Type: Journal Article | Journal: International journal of medicinal mushrooms | Year: 2016

Auricularia species are well known for their traditional edible and medicinal importance. In this study, sequence-related amplified polymorphism (SRAP) markers were used to evaluate the genetic diversity and relationships of 24 Auricularia strains obtained from different ecological regions of China. A total of 402 polymorphic loci were detected using 11 SRAP primer combinations with 100% polymorphic loci. The similarity coefficient ranged from 0.662 to 0.903, with an average of 0.73. Cluster analysis using the pair-group method using arithmetic average, based on the data from SRAP loci amplified by 11 primer pairs, showed that 24 Auricularia strains were distinguished into 4 groups with 73% similarity. The results of principal coordinate analysis were in accordance with pair-group method using arithmetic average clustering, and the first, most informative coordinate accounted for 74.38% of all the variations, which indicated a high level of genetic diversity among Auricularia strains. In general, SRAP markers provide a powerful tool to discriminate Auricularia strains and to evaluate their genetic relationships.

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