Heilongjiang Academy of Forestry

Harbin, China

Heilongjiang Academy of Forestry

Harbin, China
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Yin D.,Northeast Forestry University | Deng X.,Heilongjiang Academy of Forestry | Chet I.,Hebrew University of Jerusalem | Song R.,Northeast Forestry University
Current Microbiology | Year: 2014

The effects of the interaction between Suillus luteus (L.) Roussel and Trichoderma virens (J.H. Mill., Giddens & A.A. Foster) Arx on Pinus sylvestris var. mongolica Litv. were studied using plant physiology, mycorrhizal science, forest pathology, and biochemistry. Seedling growth and physiological parameters were determined, including the colonization rate of mycorrhizal fungi, biomass, root activity, photosynthetic pigment content, soluble protein content, antioxidant enzyme activities, rhizosphere soil enzyme activities, and protective enzyme activities. In addition, an optimal resistance system involving T. virens, mycorrhizal fungus (S. luteus), and P. sylvestris var. mongolica seedlings was constructed. Synergies between S. luteus and T. virens were observed, and most of the parameters of P. sylvestris var. mongolica seedlings inoculated with S. luteus 30 days + T. virens were higher than other treatments. After three months, when compared the control, the S. luteus 30 days + T. virens treatment gave increases in height (42.3 %); collar diameter (66.7 %); fresh weight (54 %); dry weight (50 %); soluble protein content (69.86 %); root activity (150 %); chlorophyll a (77.6 %); chlorophyll b (70.5 %); carotenoids (144 %); CAT activity (876.9 %); POD activity (268.3 %); SOD activity (66.18 %); β-1,3-glucanase activity (125.8 %); chitinase activity (40 %); rhizosphere soil catalase activity (97.8 %); and phosphatase activity (266.7 %). These results indicate that there may be a stimulating factor between S. luteus and T. virens when they are inoculated together (S. luteus 30 days + T. virens). © 2014 Springer Science+Business Media.


Liu Y.,Beijing Normal University | Liu Y.,Heilongjiang Academy of Forestry | Chen H.,Beijing Normal University | Chen H.,University of Leipzig | And 2 more authors.
Journal of Forestry Research | Year: 2017

Ectomycorrhizal (EM) networks provide a variety of services to plants and ecosystems include nutrient uptake and transfer, seedling survival, internal cycling of nutrients, plant competition, and so on. To deeply their structure and function in ecosystems, we investigated the spatial patterns and nitrogen (N) transfer of EM networks using 15N labelling technique in a Mongolian scotch pine (Pinus sylvestris var. mongolica Litv.) plantation in Northeastern China. In August 2011, four plots (20 × 20 m) were set up in the plantation. 125 ml 5 at.% 0.15 mol/L 15NH4 15NO3 solution was injected into soil at the center of each plot. Before and 2, 6, 30 and 215 days after the 15N application, needles (current year) of each pine were sampled along four 12 m sampling lines. Needle total N and 15N concentrations were analyzed. We observed needle N and 15N concentrations increased significantly over time after 15N application, up to 31 and 0.42%, respectively. There was no correlation between needle N concentration and 15N/14N ratio (R2 = 0.40, n = 5, P = 0.156), while excess needle N concentration and excess needle 15N/14N ratio were positively correlated across different time intervals (R2 = 0.89, n = 4, P < 0.05), but deceased with time interval lengthening. Needle 15N/14N ratio increased with time, but it was not correlated with distance. Needle 15N/14N ratio was negative with distance before and 6th day and 30th day, positive with distance at 2nd day, but the trend was considerably weaker, their slop were close to zero. These results demonstrated that EM networks were ubiquitous and uniformly distributed in the Mongolian scotch pine plantation and a random network. We found N transfer efficiency was very high, absorbed N by EM network was transferred as wide as possible, we observed N uptake of plant had strong bias for 14N and 15N, namely N fractionation. Understanding the structure and function of EM networks in ecosystems may lead to a deeper understanding of ecological stability and evolution, and thus provide new theoretical approaches to improve conservation practices for the management of the Earth’s ecosystems. © 2017 Northeast Forestry University and Springer-Verlag GmbH Germany


Zhao X.,Heilongjiang Academy of Forestry | Lu J.,Chinese Academy of Forestry | Jiang J.,Chinese Academy of Forestry | Wang H.,Heilongjiang Academy of Forestry | Huang Z.,Heilongjiang Academy of Forestry
Jianzhu Cailiao Xuebao/Journal of Building Materials | Year: 2014

Flexural properties of larch(larix gmelini) dimension lumber(40mm×65mm×4000mm, 40mm×90mm×4000mm and 40mm×140mm×4000mm, Ic and IIIc grade) were tested by using the method of America society for testing and materials ASTM D4761-05. The purpose of the present study is to calculate the size effect parameters of modulus of rupture for the dimension lumber of larch when a span-to-depth ratio of 18:1 was adopted for bending specimens. The results indicated that the size effect parameters is different between grades. For Ic grade the size effect parameters is 0.43 and for IIIc grade lumber the relation between size effect parameters and strength level showed linear relationship.


Zhou Z.,Northeast Forestry University | Zhou Z.,Heilongjiang Academy of Forestry | Xu M.,Northeast Forestry University | Yang Z.,Northeast Forestry University | And 2 more authors.
Journal of Reinforced Plastics and Composites | Year: 2014

Chopped carbon fibers reinforced wood plastic composites were fabricated using a two-step extrusion process. As a coupling agent, maleic anhydride polyethylene was added for improving the interfacial adhesion between the chopped carbon fibers and plastic matrix. The results showed that the mechanical properties of the maleic anhydride polyethylene-added composites were significantly improved compared to that without maleic anhydride-grafted polyethylene. Tensile strength, flexure strength and impact strength were increased by 97%-133%, 113%-119% and 181%-251%, respectively, which were very close to the strengths of structural timber. The adding of maleic anhydride polyethylene also influenced the electrical property of chopped carbon fibers reinforced wood plastic composites. The volume electrical resistivity of the composites with maleic anhydride polyethylene was higher. The scanning electron microscopy was used to examine the morphology of brittle fracture cross-section paralleled to the extrusion direction. It was observed that the interfacial adhesion was improved with the incorporation of maleic anhydride polyethylene. Chopped carbon fibers were coated by high-density polyethylene and the bonding connections were formed on the fiber surfaces. The distribution direction of chopped carbon fibers was parallel to the extrusion direction and dispersed more uniformly in the maleic anhydride polyethylene-added composites. © The Author(s) 2014.


Yin D.,Harbin Institute of Technology | Deng X.,Heilongjiang Academy of Forestry | Song R.,Harbin Institute of Technology
Journal of Forestry Research | Year: 2015

Korean spruce (Picea koraiensis Sieb. El Zucc.) is one of the main afforestation species in northern China. Seedling quality is a critical factor at planting time. To test whether the synergistic growth enhancement of Scotch pine (P. sylvestris var. mongolica) seedlings brought by the plant beneficial fungus Trichoderma virens (J.H. Mill., Giddens and A.A. Foster) Arx and ectomycorrhizal fungus (Suillus luteus (L.) Roussel.) can also benefit Korean spruce seedlings, we examined the effects of S. luteus and T. virens on the growth of P. koraiensis seedlings and drought resistance of P. sylvestris var. mongolica in peat soils. The two fungi were added to sterilized peat soil in pots, and the plants were grown for 4 months. Seedling growth and physiological variables, including mycorrhizal colonization rate of roots, biomass, and chlorophyll content, were examined. The colonization rate of the mycorrhizal fungus on P. koraiensis exceeded 65 %, and the synergism between S. luteus and T. virens enhanced most of the variables for P. koraiensis seedlings after inoculation with S. luteus then 30 days later with T. virens as in our published results for seedlings of P. sylvestris var. mongolica. When seedlings of P. sylvestris var. mongolica were inoculated with this sequence, they became more drought tolerant. T. virens also induced S. luteus to produce -1,3-glucanase and chitinase. This inoculation sequence at planting can thus improve the quality of P. sylvestris var. mongolica and P. koraiensis seedlings and substantiates our previous results. © 2015 Northeast Forestry University and Springer-Verlag Berlin Heidelberg


Wang S.,Wildlife Institute of Heilongjiang Province | Xu F.-B.,Heilongjiang Academy of Forestry | Zhu L.-F.,Wildlife Institute of Heilongjiang Province | Yu Y.-Z.,Wildlife Institute of Heilongjiang Province | Na C.-Z.,Wildlife Institute of Heilongjiang Province
Advanced Materials Research | Year: 2013

The aim of this work was to determine the inhibitory effect of deer blood hydrolysates addition on lipid oxidation, color deterioration and microbial contamination of ground beef during during 9 days of refrigerated storage. The pH value, total plate count, thiobarbituric acid-reactive substances value (TBARS), total volatile basic nitrogen (TVB-N) and color were also determined. The results showed that the treatments added hydrolysates had significant inhibitory effects on lipid oxidation and kept the red color of ground beef in 9 days, when compared with controls (P < 0.05). Furthermore, the inhibitory effect enhanced as the adding level of the hydrolysates increased (P < 0.05). These results suggest that deer blood hydrolysates are good source of natural antioxidants. © (2013) Trans Tech Publications, Switzerland.


Hebblewhite M.,University of Montana | Zimmermann F.,KORA | Li Z.,Northeast Normal University | Miquelle D.G.,Wildlife Conservation Society | And 7 more authors.
Animal Conservation | Year: 2012

The future of wild tigers is dire, and the Global Tiger Initiative's (GTI) goal of doubling tiger population size by the next year of the tiger in 2022 will be challenging. The GTI has identified 20 tiger conservation landscapes (TCL) within which recovery actions will be needed to achieve these goals. The Amur tiger conservation landscape offers the best hope for tiger recovery in China where all other subspecies have most likely become extirpated. To prioritize recovery planning within this TCL, we used tiger occurrence data from adjacent areas of the Russian Far East to develop two empirical models of potential habitat that were then averaged with an expert-based habitat suitability model to identify potential tiger habitat in the Changbaishan ecosystem in Northeast China. We assessed the connectivity of tiger habitat patches using least-cost path analysis calibrated against known tiger movements in the Russian Far East to identify priority tiger conservation areas (TCAs). Using a habitat-based population estimation approach, we predicted that a potential of 98 (83-112) adult tigers could occupy all TCAs in the Changbaishan ecosystem. By combining information about habitat quality, connectivity and potential population size, we identified the three best TCAs totaling over 25000km2 of potential habitat that could hold 79 (63-82) adult tigers. Strong recovery actions are needed to restore potential tiger habitat to promote recovery of Amur tigers in China, including restoring ungulate populations, increasing tiger survival through improved anti-poaching activities, land-use planning that reduces human access and agricultural lands in and adjacent to key TCAs, and maintaining connectivity both within and across international boundaries. Our approach will be useful in other TCLs to prioritize recovery actions to restore worldwide tiger populations. Animal Conservation © 2012 The Zoological Society of London.


Wang Y.C.,Northeast Forestry University | Qu G.Z.,Northeast Forestry University | Li H.Y.,Heilongjiang Academy of Forestry | Wu Y.J.,Northeast Forestry University | And 3 more authors.
Molecular Biology Reports | Year: 2010

Superoxide dismutases (SODs) play important role in stress tolerance of plants. In this study, an MnSOD gene (TaMnSOD) from Tamarix androssowii, under the control of the CaMV35S promoter, was introduced into poplar (Populus davidiana 9 P. bolleana). The physiological parameters, including SOD activity, malondialdehyde (MDA) content, relative electrical conductivity (REC) and relative weight gain, of transgenic lines and wild type (WT) plants, were measured and compared. The results showed that SOD activity was enhanced in transgenic plants, and the MDA content and REC were significantly decreased compared to WT plants when exposed to NaCl stress. In addition, the relative weight gains of the transgenic plants were 8- to 23-fold of those observed for WT plants after NaCl stress for 30 days. The data showed that the SOD activities that increased in transgenic lines are 1.3-4-folds of that increased in the WT plant when exposed to NaCl stress. Our analysis showed that increases in SOD activities as low as 0.15-fold can also significantly enhance salt tolerance in transgenic plants, suggesting an important role of increased SOD activity in plant salt tolerance. © Springer Science+Business Media B.V. 2009.


Yin D.,Northeast Forestry University | Deng X.,Heilongjiang Academy of Forestry | Song R.,Northeast Forestry University
Journal of Forestry Research | Year: 2016

Korean spruce (Picea koraiensis Sieb. El Zucc.) is one of the main afforestation species in northern China. Seedling quality is a critical factor at planting time. To test whether the synergistic growth enhancement of Scotch pine (P. sylvestris var. mongolica) seedlings brought by the plant beneficial fungus Trichoderma virens (J.H. Mill., Giddens and A.A. Foster) Arx and ectomycorrhizal fungus (Suillus luteus (L.) Roussel.) can also benefit Korean spruce seedlings, we examined the effects of S. luteus and T. virens on the growth of P. koraiensis seedlings and drought resistance of P. sylvestris var. mongolica in peat soils. The two fungi were added to sterilized peat soil in pots, and the plants were grown for 4 months. Seedling growth and physiological variables, including mycorrhizal colonization rate of roots, biomass, and chlorophyll content, were examined. The colonization rate of the mycorrhizal fungus on P. koraiensis exceeded 65 %, and the synergism between S. luteus and T. virens enhanced most of the variables for P. koraiensis seedlings after inoculation with S. luteus then 30 days later with T. virens as in our published results for seedlings of P. sylvestris var. mongolica. When seedlings of P. sylvestris var. mongolica were inoculated with this sequence, they became more drought tolerant. T. virens also induced S. luteus to produce -1,3-glucanase and chitinase. This inoculation sequence at planting can thus improve the quality of P. sylvestris var. mongolica and P. koraiensis seedlings and substantiates our previous results. © 2015, Northeast Forestry University and Springer-Verlag Berlin Heidelberg.


Liu X.,University of Helsinki | Hui C.,Heilongjiang Academy of Forestry | Bi L.,Heilongjiang Academy of Forestry | Romantschuk M.,University of Helsinki | And 4 more authors.
Applied Soil Ecology | Year: 2016

The Grain for Green (GFG) Project in China is currently the largest environmental rehabilitation project aimed at turning low-yielding farm land to forests and pastures. Such conversion of land use type also promotes remediation of the polluted environment. Soil microbes reflect soil function and are therefore considered an essential component of ecosystem restoration. To evaluate the environmental effects of converting atrazine polluted farmland to secondary forest, we determined soil chemical properties, soil bacterial communities and their responses to three types of land use (primary forest, PF; secondary forest, SF; farm land, FL) in Wuying, China. Our results showed that soil organic matter significantly decreases in the order PF > SF > FL. Bacterial 16S rRNA gene 454 pyrosequencing revealed that the soil bacterial diversity level remained unchanged. However between FL and the two forested sites, we observed an increase of Actinobacteria, β-proteobacteria and Firmicutes; and a decrease of Acidobacteria and Verrucomicrobia, while in SF the bacterial community structure was similar to PF. We conclude that reforestation permits bacterial community, resetting from FL back to a state that resembles natural conditions. In addition, 20 years of natural attenuation degraded soil atrazine residues in SF but traces remained in the soil. Reforestation generally resulted in favorable ecological impacts on soil quality and the bacterial community compared with active farm fields. © 2015 Elsevier B.V.

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