Heilongjiang Academy of Agriculture science

Harbin, China

Heilongjiang Academy of Agriculture science

Harbin, China
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Li X.,Northeast Forestry University | Pang H.,Northeast Forestry University | Zhao Y.,Northeast Forestry University | Sun M.,Heilongjiang Academy of Agriculture science | And 5 more authors.
Scandinavian Journal of Forest Research | Year: 2017

The aim of this study was to compare soil bacterial communities in the Great Xing’an Mountains that represent three dominant vegetation types (Quercus mongolica forest, shrub mixed with herb and grassland). Soil bacterial communities were analyzed by both culture-dependent physiological profiling (Biolog) and culture-independent DNA-based approaches. The Q. mongolica forest and shrub mixed with herb had higher average well color development than the grassland, and the Q. mongolica forest and shrub mixed with herb soil bacterial communities easily utilized miscellaneous and amines/amides. The bacterial community structure was distinct across the three sites. Most of Acidobacteria, Proteobacteria and Bacteroidetes were found in grassland soil, while Firmicutes was present at a higher percentage in the Q. mongolica soil. Extracellular enzyme assays indicated that the soil ecosystem in the grassland experienced altered N and P nutrient cycling dynamics. pH, available phosphorus, potassium and nitrogen were important in shaping bacterial community structure. These results suggest that vegetation type was a strong determinant of the structure and function of bacterial communities, which may subsequently lead to significant changes in ecosystem functioning. © 2017 Informa UK Limited, trading as Taylor & Francis Group


Wu Y.,Harbin Normal University | Gao H.,Harbin Normal University | Zhang B.,Heilongjiang Academy of Agricultural science | Zhang H.,Heilongjiang Academy of Agriculture science | And 4 more authors.
Scientia Agricultura Sinica | Year: 2017

[Objective]The aim of this experiment was to study the effects of exogenous EBR (24-epibrassinolide) on the growth indices, physiological characteristics and cellular ultrastructure of soybean under saline-alkali stress. [Method]Soybean variety Heinong 44 was employed as the test material, and cultivated in 110 mmolL-1 nutrient solution saline-alkali stress treatments for 3 d and 7 d to investigate the effects of 1.2 mg·L-1 exogenous EBR on the height and root growth, SOD, POD, and APX three kinds of antioxidant enzyme activities, relative electrical conductivity, superoxide anion (O2 -) production rate, contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA), free proline concentration content, chlorophyll content of soybean leaves and the responses of cellular ultrastructure of soybean leaves and root tips under saline-alkali stress. [Result] Under the condition of saline-alkali stress dealing with 3 d and 7 d, compared to the control, the three kinds of antioxidant enzyme activities of SOD, POD, and APX, free proline content, relative electrical conductivity, O2 - production rate, contents of H2O2 and MDA all increased, the growth indices and chlorophyll content all decreased; the chloroplast and mitochondria cellular ultrastructure of soybean leaves suffered severe damages. The mitochondria and endoplasmic reticulum cellular ultrastructure of soybean root tips suffered severe damages, and vacuole was ruptured. Application of EBR under saline-alkali stress increased soybean plant height, root length, fresh mass of root by 6.45%, 9.60% and 19.85%, respectively. Application of EBR the activities of SOD, POD, and APX of soybean leaves in 3 d and 7 d treatments increased by 16.92% and 9.68%, 48.85% and 61.44%, 19.05% and 20.36%, respectively, relative electrical conductivity, O2 - production rate, H2O2 and MDA contents significantly decreased by 19.58% and 28.26%, 28.06% and 40.92%, 28.62% and 31.21%, 31.03% and 37.17%, respectively, free proline content and chlorophyll content significantly increased by 3.67% and 15.96%, 13.34% and 16.87%, respectively. At the same time, the stability of soybean leaves and root tip cell ultrastructure were maintained and the aging of cells and disintegration were delayed.[Conclusion]The results suggested that application of exogenous EBR under saline-alkali stress could increase the antioxidant enzyme activities, free proline concentration and chlorophyll content, reduce the accumulation of reactive oxygen species(ROS), maintain the cell structure integrity, promote seedling growth and resulting in improving the tolerance of soybean seedlings to saline-alkali stress.


Qin J.,Chinese Academy of Agricultural Sciences | Qin J.,Hebei Academy of Agricultural and Forestry Science | Yang R.Q.,Shanghai JiaoTong University | Jiang C.X.,Heilongjiang Academy of Agriculture Science | And 5 more authors.
Plant Breeding | Year: 2010

With 3 figures and 5 tablesIn this study, we extended in silico mapping for single trait to analyse data from multiple environments by calculating intraclass correlations and to mapping pleiotropic QTL for multiple traits by defining new statistic to measure the correlation between multiple traits and the marker. Data sets include phenotypes of eight agronomic traits obtained from six different ecologic environments and years, and genotypic information from 477 polymorphic markers on 14 ancestral lines in the pedigree of 'Suinong14'. With in silico mapping, a total of 39 markers distributed on 14 linkage groups are detected as QTL responsible for eight agronomic traits and 10 QTL are identified as having pleiotropic effects. Tracing transmission of functional QTL in the pedigree indicated that certain QTL, such as Sat_036 on linkage group D1a, Satt182 on linkage group L, and Satt726 on linkage group B2 may be responsible for the contribution of exotic germplasm to the improved cultivars. © 2010 Blackwell Verlag GmbH.


Jin L.,China Agricultural University | Jin L.,Heilongjiang Academy of Agriculture science | Hu K.,China Agricultural University | Deelstra J.,Institute for Agricultural and Environmental Research Bioforsk As | And 3 more authors.
Acta Agriculturae Scandinavica Section B: Soil and Plant Science | Year: 2014

Water scarcity and nitrate contamination in groundwater are serious problems in the North China Plain (NCP). The objective of this study was to compare the effects of farmer's practice and optimal water and nitrogen management on nitrate leaching, and water and nitrogen use efficiencies (WUE, NUE) during winter wheat season in the NCP. A winter wheat experiment with four treatments (traditional irrigation plus traditional fertilizer, W1N1; traditional irrigation plus optimal fertilizer, W1N2; optimal irrigation plus traditional fertilizer, W2N1; optimal irrigation plus optimal fertilizer, W2N2) was conducted from October 1999 to June 2001 in Beijing suburban. The soil-plant system, water and solute transport model was calibrated and validated based on the data collected from the experimental field, then the model was used to simulate the water movement, nitrogen (N) transport, and crop growth process. The results showed that the simulated soil water content, nitrate concentration in the soil profile, leaf area index, crop N uptake, and grain yield were all in good agreement with the measured data. The simulated results indicated that the improved management of water and fertilizer practice could increase crop yield, and reduce water drainage, nitrate leaching, and gaseous N loss compared to farmers' practice. WUE and NUE under improved practice were 1.36 kg m-3 and 34.9 kg kg-1N-1 in 2000, 1.07 kg m-3 and 31.5 kg kg-1N-1 in 2001, respectively. Compared to farmers' practice, the optimal management practice can save water about 4-19%, water drainage decreased 50-65%, N leaching reduced over 90% and gaseous N loss decreased about 70%, at same time leading to an increase in WUE and NUE by 10.6% and 10.3% in 2000, 64.5% and 98.3% in 2001, respectively. © 2014 Taylor & Francis.


Fu B.,Harbin University | Liu D.,Heilongjiang Academy of Agricultural Science | Liu D.,Harbin University | Fang Q.-C.,Harbin University | And 5 more authors.
Zygote | Year: 2011

Transgenes integrated into mammalian cells are silenced rapidly. This phenomenon correlates with repressed chromatin structure marked by histone hypoacetylation. This study investigated the effect of trichostatin A (TSA; a histone-deacetylase inhibitor) on EGFP expression in transfected cells and embryonic development after somatic cell nuclear transfer (SCNT). Porcine adult fibroblasts were transfected with a pEGFP-C1 vector. Then transfected cells, donor cells for SCNT, were pretreated with TSA, with the untreated cells being used as the control. Expression of EGFP in donor cells and reconstructed embryos was detected when exposed to blue light. Results showed that the percentage of EGFP-positive cells significantly increased when the transfected cells were treated with TSA and the increased expression of EGFP was sustained to at least the morula stage. In addition, the cytotoxic effect of TSA on the transfected cells was dose dependent. In conclusion, TSA can rescue the silenced EGFP gene. Even after transferring the TSA-treated cells to enucleated recipient oocytes, TSA retained the ability to rescue a silenced EGFP gene. In addition, TSA had an impact on cell proliferation. © Copyright Cambridge University Press 2010.


Jin J.,Chinese Academy of Sciences | Liu X.,Chinese Academy of Sciences | Wang G.,Chinese Academy of Sciences | Mi L.,Chinese Academy of Sciences | And 3 more authors.
Field Crops Research | Year: 2010

Increasing yield is a high priority in most breeding programs. Approximately 600 soybean cultivars had been released by the end of the last century in Northeast China. Understanding the agronomic and physiological changes is essential for planning further plant breeding strategies in soybean. In this study, 45 representative soybean cultivars, from maturity groups 00 and 0, released from 1950 to 2006 in Northeast China were compared in field conditions for 3 consecutive years. A positive correlation between seed yield and year of cultivar release was indicated with a 0.58% average annual increase. Seed number per plant was the most important contributor to yield gain, with a 0.41% increase per year. Pod number per plant and seed size varied slightly with the year of cultivar release. Although variation in protein was from 37.0% to 45.5%, and oil concentration was from 16.7% to 22.0%, their concentrations were not consistently related to year of cultivar release. A 33% increase in the photosynthetic rate, 10.6% increase in plant dry weight and 19.0% increase in harvest index (HI) were found, while leaf area index (LAI) decreased by 17.3%. Modern cultivars have higher photosynthetic rates than their predecessors. The reduced plant height gave increased resistance to lodging, with the lodging score dropping from 3.2 in 1951 to 1.0 in 2006. Seed resistances to disease and pest infestation were also improved. Yield stability was enhanced over years, which could be attributed to the stable pod production across different environments. A flow diagram to explain the contributors to genetic improvement of soybeans in Northeast China was developed. © 2009 Elsevier B.V. All rights reserved.


Zhang D.J.,Heilongjiang Academy of Agriculture Science | Liu D.,Heilongjiang Academy of Agriculture Science | Yang G.W.,Heilongjiang Academy of Agriculture Science | FU X.K.,Heilongjiang Academy of Agriculture Science | HE X.M.,Heilongjiang Academy of Agriculture Science
Acta Agriculturae Scandinavica A: Animal Sciences | Year: 2010

The effects of the polymorphism of the nuclear receptor coactivator 1 gene (NCOA1), osteopontin (OPN), and retinolbinding protein 4 (RBP4) genes on individual weight at birth (WTB) and individual weight at 30 days (WT30) were analyzed by Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in hybrid pig (Landrace ♀ Wild-boar ♂). The relationship between different genotypes and WTB and WT30 were analyzed. Results showed that: AA genotype of NCOA1 had higher WT30 than BB individuals (PB<0.05). AA and AB genotypes of OPN had a higher WTB and WT30 than BB (P>0.05). BB and AB genotypes of RBP4 had a higher WTB and WT30 than AA (P>0.05). The results presented here demonstrate that the NCOA1 gene may be considered as a marker for WT30 trait in pig. © 2010 Taylor & Francis.


Zhang D.-J.,Heilongjiang Academy of Agriculture Science | Liu D.,Heilongjiang Academy of Agriculture Science
Journal of Applied Animal Research | Year: 2010

Polymorphism in Estrogen Receptor a (ESRα), Prolactin Receptor (PRLR), Luteinizing Hormone β (LHβ) and the Ryanodine Receptor I (RYRI) genes, which play important roles in litter size of pigs, were analysed in four pig populations (Min, Landrace, York-shine, Duroc) using PCR-SSCP. There was one SNP in ESRα (Arg/His 488), two SNPs in PRLR (one silent mutation and Val/Ala 555) and two silent mutations each in LHβ and RYRI. PRLR was highly polymorphic locus (0.59≥PIC≥0.46), LBβ and RYRI were moderately polymorphic loci (0.36≥PIC≥0.15) and ESRβ was very low polymorphic locus (0.07≥PIC≥0.00). © GSP, India.


Dong-jie Z.,Heilongjiang Academy of Agriculture Science | Di L.,Heilongjiang Academy of Agriculture Science | Liang W.,Heilongjiang Academy of Agriculture Science | Wen-tao W.,Heilongjiang Academy of Agriculture Science | And 2 more authors.
Journal of Applied Animal Research | Year: 2016

Low-temperature injury is one of the most significant causes of livestock damage worldwide. To identify genes that respond to cold stress, the transcriptome profile of the skeletal muscle of the Min pig (Sus scrofa) after cold stress was analysed using the deep sequencing technique. One group was raised in the normal environment (18 ± 2°C, n = 3) as the thermos-neutral group; another group was raised in the low-temperature environment (4 ± 2°C, n = 3) as the cold stress group. After 24 h, the gene expression profile of skeletal muscle was analysed. The results showed that the expression levels of 662 genes were significantly up-regulated, and those of 1058 genes were significantly down-regulated in the thermos-neutral and the cold stress groups (P < 0.01). It was found through Gene Ontology analysis that the significantly differently expressed genes were mainly distributed in the cytoplasm, mitochondrion, organelle envelope and ribosomal subunit. They enriched ribosome, fatty acid metabolism, RNA transport and metabolic pathways. It means that cold stress could significantly change the gene expression profile of pig muscle. © 2016 The Author(s) Published by Taylor & Francis


PubMed | Heilongjiang Academy of Agriculture Science
Type: Journal Article | Journal: Sheng wu gong cheng xue bao = Chinese journal of biotechnology | Year: 2011

To screen the interactive proteins with IBDV Gt VP2 protein from cDNA library of B Lymphoid cells of the bursa of Fabricius. The expression cDNA library plasmids was transformed to the yeast competent cells, which have the bait plasmid-Gt VP2. After testing for growth in synthetic complete medium lacking histidine and uracil and for production of beta-galactosidase (X-gal), we obtained 16 positive clones. We searched the gene sequences of positive clones in the NCBI website. The blast results showed that five positive clones were the gallus sequences. They were Gallus gallus breed mitochondrial DNA, O_G1cNAc transferase, Tumor protein p53 binding protein, Stathmin and Chondroitin sulfate Ga1NAcT-2, respectively. This study is helpful for the further identifying the receptors of IBDV in B Lymphoid cells of the bursa of Fabricius.

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