Hefei, China
Hefei, China

Time filter

Source Type

Fang F.,Anhui Agricultural University | Zhou X.,Anhui Agricultural University | Li F.,Anhui Agricultural University | Zhang F.L.,Hefei Wild Animal Park | Jiang S.,Anhui Agricultural University
Journal of Animal and Veterinary Advances | Year: 2012

Glucagon is the hyperglycemic factor that opposes the action of insulin. It stimulates the breakdown of liver glycogen and increased glucogeogenesis. Glucagon is located in the tissues of the pancreas of many animals. However, the distribution of glucagon has not been investigated in the pancreas of the tiger. The present study was performed to describe the histological structure of the liver and pancreas and the location of glucagon of pancreas in the 8 days old Siberian tiger. The tissues of the liver and pancreas were collected from three 8 days old Siberian tigers that died of starvation. The weight was recorded and the histological sections were made and stained with HE. The localization of glucagon in tissue was detected by immunohistochemical two-step PV-9000. The results show that the average weight of the liver and pancreas was approximately 39.06 and 1.97 g, respectively. The tissue structure of the liver consisted of hepatic lobulation and portal area. Central vein, hepatocyte tube and hepatic sinusoid and lymphatic tissue were found in hepatic lobulation. Interlobular veins, interlobular artery and interlobular bile duct were presented in portal area. The pancreas was basically made up of exocrine portion and pancreas islet. The exocrine secretion of the pancreas included many pancreatic acinuses and centroacinar cell was observed in the pancreatic acinus. Glucagon-immunopositive cells were mainly located in the pancreas islet. The current study preliminarily revealed the histological features of the liver and pancreas and the distribution of glucagon in the pancreas of 8 days old Siberian tiger which requires further investigation. © Medwell Journals, 2012.


Zhang Y.,Anhui Agricultural University | Li Y.,Anhui Agricultural University | Zhang Z.,Hefei Wild Animal Park | Cao Z.,Anhui Agricultural University | And 4 more authors.
Journal of Animal and Veterinary Advances | Year: 2012

Substance P (SP) belongs to the tachykinin family and plays important roles in many physiological processes. The distribution of SP has not been investigated in the digestive tract of the tiger. The present study was performed to describe the histological structure and location of SP in the digestive tract which included the esophagus, stomach, small intestine and large intestine of the Siberian tiger at 8 days of age. The tissues of the digestive tract were collected from three 8 days old Siberian tigers that died of cold weather. The weight was recorded and the histological sections were made and stained with HE. The localization of SP in tissue was detected by lmmunohistochemical two-step PV-9000. The results show that the length of the digestive tract was approximately 215.6 cm with an average weight of 48.95 g. The wall of the digestive tract consisted of mucosa, submucosa, unica muscularis and tunica adventitia. The epithelium of the esophagus was a stratified squamous type without beginning surface cornification and the unica muscularis consisted of skeletal muscle. The lamina propria of the stomach was located by many glands but did not reach the muscularis mucosa. SP-immunoreactive cells were identified primarily in the epithelium and the cardiac gland of the stomach. SP-immunoreactive cells were also found in the intestinal gland of the lamina propria of the cecum and the colon. The current study preliminarily revealed the histological features and the distribution of SP in the digestive tract of an 8 days old Siberian tiger which requires further investigation. © Medwell Journals, 2012.


Li F.-Y.,Anhui Agricultural University | Tao Y.,Anhui Agricultural University | Fang F.-G.,Anhui Agricultural University | Li Y.-S.,Anhui Agricultural University | And 3 more authors.
Animal Biology | Year: 2010

The present study was carried out to describe the reproductive system of a single adult female wolf, including the uterine horns, cervix, ovaries and follicles. The cumulus oocytes complexes (COCs) and oocytes were also examined. The results showed that the size of each ovary was about 9 × 6 mm with an average of weight of 461.3 mg. The uterus was Y-shaped, and the length of each uterine horn was 14 cm. The distance from the cervix to the bifurcation of the uterine horns was also 14 cm. The left ovary had two large follicles on the surface with a diameter more than 4 mm, while the right ovary had no protuberant follicles. The ovaries were covered with a lot of fat, and were well developed. The COCs derived from the antral follicles were dark, and the nuded oocytes had a dark cytoplasm. The diameter of the oocytes removed from the antral follicles was 116.8 μm on average. The ovaries had a smooth surface and all the follicles were under the surface except for two big follicles on the left ovary. Histological examination of the ovaries by haematoxylin and eosin staining demonstrated that the primordial, primary, preantral and antral follicles were scattered in the cortex, the medulla was abundant with blood vessels. This study preliminarily reveals the features of the wolf reproductive system and the structure of its oocytes and ovaries, which might be indicative for further study and the protection of the species. © 2010 Koninklijke Brill NV.

Loading Hefei Wild Animal Park collaborators
Loading Hefei Wild Animal Park collaborators