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Wei W.,Jilin University | Guo H.,Tianjin University | Li J.,Jilin University | Wei Z.,Jilin University | And 8 more authors.
PLoS ONE | Year: 2014

Human enteroviruses (HEV) have been linked to hand, foot, and mouth disease (HFMD) in the Pacific and Southeast Asia for decades. Many cases of HFMD have been attributed to coxsackievirus A16 (CV-A16, CA16), based on only partial viral genome determination. Viral phenotypes are also poorly defined. Herein, we have genetically and phenotypically characterized multiple circulating CV-A16 viruses from HFMD patients and determined multiple full-length sequences of these circulating viruses. We discovered that the circulating CV-A16 viruses from HFMD patients are genetically distinct from the proto-type CV-A16 G10. We have also isolated circulating CV-A16 viruses from hospitalized HFMD patients and compared their virological differences. Interestingly, circulating CV-A16 viruses are more pathogenic in a neonatal mouse model than is CV-A16 G10. Thus, we have found circulating recombinant forms of CV-A16 (CRF CV-A16) that are related to, but different from, the prototype CV-A16 G10 that have distinct biological phenotypes.©2014 Wei et al.


Wang D.-G.,Anhui Medical University | Tang X.-W.,HeFei Vocational and Technical College | Fan Y.,Shanxi Medical University | Leng R.-X.,Anhui Medical University | And 6 more authors.
Inflammation | Year: 2014

Premature atherosclerosis, the hallmark of cardiovascular diseases, has been found to be a significant cause of late deaths in systemic lupus erythematosus (SLE) patients. Therefore, early identification of atherosclerosis before the overt disease is curial for the management program of SLE. Flow-mediated dilatation (FMD%) is a reliable, noninvasive, easy to use, reproducible, and pathogenically relevant index for early atherosclerosis. In recent years, a number of studies have been performed to compare the mean FMD% difference between patients with SLE and healthy controls. However, these studies have shown inconclusive or even contradictory findings. In this study, to derive a more precise comparison of FMD% difference between SLE patients and healthy controls, a meta-analysis was performed. Databases were searched to identify all available studies comparing FMD% between SLE patients and healthy controls. The study eligibility criteria were cohort or case–control studies with data on both patients diagnosed with SLE and healthy controls, and use of high-resolution ultrasonography to detect FMD. Random effect meta-analysis was conducted to evaluate the overall mean FMD% difference between the two groups. Publication bias was detected by funnel plot and Egger’s test. Meta-regression analysis was performed to investigate the potential influencing factors on FMD% difference. Of the 434 articles initially identified, 22 were finally included in the meta-analysis. Compared to healthy controls, SLE patients had significantly lower FMD% (standardized mean difference, −1.19; 95 % CI, −1.63, −0.74; P < 0.001). There was significant heterogeneity among these studies (I2 = 94.3 %, P < 0.001), which was mainly due to variations in disease duration of SLE patients. The funnel plot showed a skewed shape, indicating a marked publication bias, which was further supported by the Egger’s test (P = 0.006). However, after the correction for potential publication bias by using the trim-and-fill method, the main results for all studies combined were still significant (P < 0.001). Taken together, these findings support the current evidence on a higher cardiovascular burden in SLE and support using FMD% as a surrogate for premature atherosclerosis in SLE patients. © 2014, Springer Science+Business Media New York.


Fan F.-T.,Nanjing University | Fan F.-T.,Hefei Vocational and Technical College | Shen C.-S.,Nanjing University | Tao L.,Nanjing University | And 11 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2014

Pyruvate kinase isozyme type M2 (PKM2) was first found in hepatocellular carcinoma (HCC), and its expression has been thought to correlate with prognosis. A large number of studies have demonstrated that epithelial-mesenchymal transition (EMT) is a crucial event in hepatocellular carcinoma (HCC) and associated metastasis, resulting in enhanced malignancy of HCC. However, the roles of PKM2 in HCC EMT and metastasis remain largely unknown. The present study aimed to determine the effects of PKM2 in EGF-induced HCC EMT and elucidate the molecular mechanisms in vitro. Our results showed that EGF promoted EMT in HCC cell lines as evidenced by altered morphology, expression of EMT-associated markers, and enhanced invasion capacity. Furthermore, the present study also revealed that nuclear translocation of PKM2, which is regulated by the ERK pathway, regulated β-catenin-TCF/LEF-1 transcriptional activity and associated EMT in HCC cell lines. These discoveries provide evidence of novel roles of PKM2 in the progression of HCC and potential therapeutic target for advanced cases.


Fan F.-T.,Nanjing University of Traditional Chinese Medicine | Fan F.-T.,Hefei Vocational and Technical College | Shen C.-S.,Nanjing University of Traditional Chinese Medicine | Pei C.-S.,Xuzhou Medical College | And 7 more authors.
Tumor | Year: 2012

HOX transcript antisense RNA (HOTAIR) is the first long non-coding RNA (lncRNA) which was found to have regulatory functions of reverse transcription. It can simultaneously bind to polycomb repressive complex 2 (PRC2) and histone demethylase complex [LSD1 (lysine specific demethylase 1)/ CoREST (Co-repressor of RE1-silencing transcription factor)/REST] and mediates the binding of these two complexes to the specific gene sites, resulting in histone H3 tri-methylated at lysine 27 (H3K27me3) and histone H3 dimethyl Lys4 (H3K4me2), and ultimately leading to gene silencing. Clinical studies have shown close relationships of the expression of HOTAIR with metastasis and recurrence of many cancers such as breast cancer, colorectal cancer and liver cancer, as well as the prognosis of the patients with these cancers. The high expression level of HOTAIR can inhibit the expression of cancer-related metastasis suppressor genes and improve the malignant transformation of tumors; conversely, HOTAIR silencing can decrease the metastatic capability of cancer cells. Copyright © 2012 by TUMOR.


Cai Y.-H.,Hefei Vocational and Technical College | Xu Z.-G.,Hefei Vocational and Technical College
Chinese Journal of Biologicals | Year: 2014

Objective To optimize the fermentation condition of nattokinase (NK) high-producing strain using Bacillus natto F3 as an original strain. Methods The effects of culture method (oscillation and stationary culture), inoculum size (0, 2, 4, 6, 8 and 10 ml), temperature for culture ( 18, 25. 30, 37, 45 °C), time for culture ( 12, 24, 36, 48, 60 and 72 h), original pH value (3. 5, 4. 5, 5. 5, 6. 5. 1.5 and 8. 5) on enzyme activity of NK were evaluated by a single factor test, based on which the major influencing factors were selected for orthogonal test so as to obtain an optimal portfolio. Results The optimal condition for culture in oscillation was 200 r / min, while the optimal inoculum size was 6%, the optimal temperature, time and pH value for culture were 37 °C, 48 h and 7. 5 respectively. Under the optimal condition. the enzyme activity of NK reached 751 U/ ml. Conclusion The fermentation condition of NK high-producing strain was optimized, which laid a foundation of increasing the yield of NK and decreasing the cost of production.


Yang L.-L.,Hefei Vocational and Technical College
Proceedings - 2013 10th International Conference on Fuzzy Systems and Knowledge Discovery, FSKD 2013 | Year: 2013

A fuzzy comprehensive evaluation model for mulberry wine quality was set up. The influencing factors of mulberry wine quality including color, flavor, taste, style and etc. were investigated comprehensively by the use of a weight distribution method and a multiply operator method. The methods used in this paper made up the defects in traditional sensory evaluation and diminished manmade subjective effects on the evaluation. The model set up in this paper, which had positive guidance in the mulberry wine scientific evaluation and significance in the development of quality mulberry wine, can get more accurate evaluation results. © 2013 IEEE.


Tang X.-W.,HeFei Vocational and Technical College | Wang J.,Anhui Medical University | Zou Y.-F.,Anhui Medical University
Nordic Journal of Psychiatry | Year: 2015

Background: In the past few decades, a number of studies have investigated the association of the wolframin (WFS1) gene H611R polymorphism with mood disorders, but the findings are not always consistent. Aims: The objective of the present study is to assess the association between WFS1 gene H611R polymorphism and mood disorders by using a meta-analysis. Methods: A comprehensive literature search of PubMed, Excerpta Medica Database, Elsevier Science Direct and China National Knowledge Infrastructure databases was conducted to identify relevant articles, with the last report up to April 15, 2014. Pooled odds ratio (OR) with 95% confidence interval (CI) was estimated. Results: Seven studies including 1318 cases and 1252 controls were selected from potentially relevant articles. This meta-analysis showed that there was no significant association between WFS1 gene H611R polymorphism and mood disorders (R vs. H: OR = 0.93, 95% CI = 0.82-1.05, P = 0.22; HR+ RR vs. HH: OR = 0.98, 95% CI = 0.82-1.17, P = 0.80; RR vs. HH+ HR: OR = 0.84, 95% CI = 0.67-1.04, P = 0.11; RR vs. HH: OR = 0.86, 95% CI = 0.67-1.10, P = 0.24; HR vs. HH: OR = 1.03, 95% CI = 0.78-1.36, P = 0.83). In subgroup analyses by ethnicity, we did not detect any significant association of this polymorphism with mood disorders in Caucasian and Asian populations (P > 0.05). In subgroup analyses by types of mood disorders, we also did not detect any significant association of this polymorphism with bipolar disorder or major depressive disorder (P > 0.05). Conclusions: The results of this meta-analysis suggest that there is no association between WFS1 gene H611R polymorphism and mood disorders. © 2014 Informa Healthcare.

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