Mao C.,Southern Medical University |
Qiu L.-X.,Fudan University |
Zhan P.,Nanjing Chest Hospital |
Xue K.,Fudan University |
And 4 more authors.
Journal of Cancer Research and Clinical Oncology | Year: 2010
Purpose: Published data on the association between manganese superoxide dismutase (MnSOD) Val16Ala polymorphism and prostate cancer (PCA) risk are inconclusive. To derive a more precise estimate of the association between them, a meta-analysis was performed. Methods: PubMed and Embase were searched. All eligible studies were retrieved. The pooled odds ratio (OR) with 95% confidence interval (CI) for PCA risk associated with Val/Ala versus Val/Val, Ala/Ala versus Val/Val, dominant model (Ala/Ala + Val/Ala vs. Val/Val), and recessive model (Ala/Ala vs. Val/Ala + Val/Val) were estimated, respectively. Results: A total of 12 studies including 8,962 subjects were involved in this meta-analysis. Overall, the meta-analysis indicated that significantly elevated cancer risk was associated with Ala variant genotype when all the eligible studies were pooled into the meta-analysis (for Val/Ala vs. Val/Val: OR = 1.11, 95% CI = 1.00-1.24; for Ala/Ala vs. Val/Val: OR = 1.22, 95% CI = 1.00-1.49; for dominant model: OR = 1.14, 95% CI = 1.03-1.26). In the subgroup analysis by ethnicity, statistically significant increased risks were found among Caucasians with Ala allele (for Val/Ala vs. Val/Val: OR = 1.12, 95% CI = 1.00-1.25; for dominant model: OR = 1.14, 95% CI = 1.02-1.26). However, no significant associations were found in Africans. Conclusions: This meta-analysis suggests that the Ala allele of the MnSOD gene was a low-penetrance susceptible gene in PCA development, especially in Caucasians. © 2010 Springer-Verlag.
PubMed | Anhui Medical University, Hefei Second Peoples Hospital and Anhui Provincial Hospital
Type: | Journal: Clinics and research in hepatology and gastroenterology | Year: 2017
Tripartite Motif Containing 11 (TRIM11), a member of TRIM proteins is overexpressed in gliomas and lung cancer. However, the role of TRIM11 in hepatocellular carcinoma (HCC) is unknown.Herein, we aimed to investigate the expression and clinical significance role of TRIM11 in HCC.In this study, our data showed significant higher TRIM11 in HCC tissues (n=117) than in the matched non-tumor liver (NTL) tissues (P<0.01). In consistent with above data, we also found TRIM11 protein expression was significantly increased compared with the matched NTL (P<0.01) by immunohistochemistry analysis. Additionally, our results showed that TRIM11 protein expression in HCC tissues was significantly associated with pathological grade (P<0.01), tumor postoperative metastasis (P=0.031), recurrence (P=0.022), and serum a-fetoprotein (AFP) (P<0.01). Moreover, patients survival was negatively correlated with TRIM11 protein expression. Furthermore, we found that TRIM11 protein was an independent prognostic factor for disease-free (P<0.01) and overall survival (P<0.01) in HCC patients.Our data showed that TRIM11 expression was significantly elevated in HCC tissues. The overexpression of TRIM11 is closely associated with HCC progression and poor survival of the patients, indicating TRIM11 is a potential therapeutic target for HCC patients.
Mao C.,Southern Medical University |
Qiu L.-X.,Fudan University |
Liao R.-Y.,Southern Medical University |
Du F.-B.,Hefei Second Peoples Hospital |
And 4 more authors.
Lung Cancer | Year: 2010
Epidemiologic studies have evaluated the association between KRAS mutations and resistance to the treatment of epidermal growth factor receptor (EGFR) tyrosine-kinase inhibitors (TKIs) in patients with non-small cell lung cancer (NSCLC). However, results were inconclusive. To derive a more precise estimation of the relationship, we performed this meta-analysis. Systematic computerized searches of the PubMed and Medline databases (up to Jun 30, 2009) were performed. A total of 22 studies were included in the final meta-analysis, consisting of 1470 NSCLC patients, of whom 231 had KRAS mutations (16%). Current or former smokers had a higher frequency of KRAS mutations than never smokers (25% versus 6%; OR = 4.36; P< 0.01). Mutations were more common among adenocarcinoma than other histologies (26% versus 16%; OR = 1.98; P< 0.01). The objective response rate (ORR) of NSCLC patients with mutant KRAS was 3% (6/210), whereas the ORR of NSCLC patients with wild-type KRAS was 26% (287/1125). The overall pooled RR for ORR was 0.29 (95% CI: 0.18-0.47; P< 0.01). Subgroup analyses were conducted on the basis of ethnicity and study treatment, all the results were not materially altered and did not draw different conclusions, indicating that our results were robust. In summary, this meta-analysis suggests that KRAS mutations may represent negative predictive biomarkers for tumor response in NSCLC patients treated with EGFR-TKIs. However, due to a mutually exclusive relationship between KRAS and EGFR mutation and no difference in survival between KRAS mutant/. EGFR wild-type and KRAS wild-type/. EGFR wild-type NSCLC, the clinical usefulness of KRAS mutation as a selection marker for EGFR-TKIs sensitivity in NSCLC is limited. © 2009 Elsevier Ireland Ltd.
PubMed | Hefei Second Peoples Hospital and Shenzhen Seventh Peoples Hospital
Type: Journal Article | Journal: Biomedical reports | Year: 2014
Chorioamnionitis is common in females with prematurely ruptured fetal membranes (PROM). The current diagnosis of PROM and preterm PROM (PPROM) is based on vaginal fluid analysis. The present study investigated the value of serum -human chorionic gonadotropin (-hCG) and interleukin-1 (IL-1) levels in diagnosing chorioamnionitis. In total, 150 term-pregnancy patients were included in the prospective study. A total of 50 females had normal pregnancies (control group) and 100 had PROM. One hour before delivery, 3 ml venous blood was collected and analyzed. Fetal membrane and placental tissue underwent histopathological analyses. Of the 100 term-pregnancy females, 56 had PROM and 44 had PROM combined with chorioamnionitis (PROM + C). The serum -hCG levels for the control, PROM and PROM + C groups were 7,557.862,922.06, 636.9614,379.10 and 50,310.3422,874.82 IU/l, respectively. The receiver operating characteristic (ROC) for PROM and PROM + C groups (-hCG 23,900.50 IU/l) had a sensitivity of 77.5% and a specificity of 78.6%. The level of IL-1 in the PROM + C group was higher compared to the control and PROM groups (0.580.05, 0.120.04 and 0.130.03 ng/ml, respectively). In conclusion, ROC for the PROM and PROM + C groups (IL-1 0.38 ng/ml) had a sensitivity of 76.5% and a specificity of 72.6%. Therefore, serum -hCG and IL-1 are potential biomarkers for diagnosing PROM and PROM + C, respectively.
Liu M.,Anhui Medical University |
Wu Q.,Anhui Medical University |
Chen P.,Anhui Medical University |
Buchele B.,University of Ulm |
And 8 more authors.
PLoS ONE | Year: 2014
Boswellic acid (BA)-containing extracts such as BSE have anti-inflammatory and immunomodulatory activity. In chronic schistosomiasis, the hepatic granuloma and fibrosis induced by egg deposition in the liver is the most serious pathological manifestations. However, little is known regarding the role of BAs in Schistosoma japonicum (S. japonicum) egg-induced liver granuloma and fibrosis. In order to investigate the effect of a water-soluble complex preparation of BSE, BSE-CD, on S. japonicum egg-induced liver pathology, liver granuloma and fibrosis were induced by infecting C57BL/6 mice with 18-22 cercariae of S. japonicum. S. japonicum cercariae infected mice were injected with BSE-CD at the onset of egg granuloma formation (early phase BSE-CD treatment after 4 weeks infection) or after the formation of liver fibrosis (late phase BSE-CD treatment after 7 weeks infection). Our data show that treatment of infected mice with BSE-CD significantly reduced both the extent of hepatic granuloma and fibrosis. Consistent with an inhibition of NF-κB signaling as evidenced by reduced IκB kinase (IKK) activation, the mRNA expression of VEGF (vascular endothelial growth factor, VEGF), TNF-α (tumor necrosis factor-alpha TNF-α) and MCP-1 (monocyte chemotactic protein 1, MCP-1) was decreased. Moreover, immunohistochemical analysis (IHC) revealed that the content of α-SMA in liver tissue of BSE-CD treated mice was dramatically decreased. Our findings suggest that BSE-CD treatment attenuates S. japonicum egg-induced hepatic granulomas and fibrosis, at least partly due to reduced NF-κB signaling and the subsequently decreased expression of VEGF, TNF-α, and MCP-1. Suppression of the activation of hepatic stellate cells (HSC) may also be involved in the therapeutic efficacy of BSE-CD. © 2014 Liu et al.
PubMed | Anhui Medical University, Hefei Second Peoples Hospital and University of Ulm
Type: Journal Article | Journal: PloS one | Year: 2014
Boswellic acid (BA)-containing extracts such as BSE have anti-inflammatory and immunomodulatory activity. In chronic schistosomiasis, the hepatic granuloma and fibrosis induced by egg deposition in the liver is the most serious pathological manifestations. However, little is known regarding the role of BAs in Schistosoma japonicum (S. japonicum) egg-induced liver granuloma and fibrosis. In order to investigate the effect of a water-soluble complex preparation of BSE, BSE-CD, on S. japonicum egg-induced liver pathology, liver granuloma and fibrosis were induced by infecting C57BL/6 mice with 18-22 cercariae of S. japonicum. S. japonicum cercariae infected mice were injected with BSE-CD at the onset of egg granuloma formation (early phase BSE-CD treatment after 4 weeks infection) or after the formation of liver fibrosis (late phase BSE-CD treatment after 7 weeks infection). Our data show that treatment of infected mice with BSE-CD significantly reduced both the extent of hepatic granuloma and fibrosis. Consistent with an inhibition of NF-B signaling as evidenced by reduced IB kinase (IKK) activation, the mRNA expression of VEGF (vascular endothelial growth factor, VEGF), TNF- (tumor necrosis factor-alpha TNF-) and MCP-1 (monocyte chemotactic protein 1, MCP-1) was decreased. Moreover, immunohistochemical analysis (IHC) revealed that the content of -SMA in liver tissue of BSE-CD treated mice was dramatically decreased. Our findings suggest that BSE-CD treatment attenuates S. japonicum egg-induced hepatic granulomas and fibrosis, at least partly due to reduced NF-B signaling and the subsequently decreased expression of VEGF, TNF-, and MCP-1. Suppression of the activation of hepatic stellate cells (HSC) may also be involved in the therapeutic efficacy of BSE-CD.
Feng J.,Anhui Medical University |
Liu H.,Hefei Second Peoples Hospital |
Chen J.,Anhui Medical University |
Wang J.,Anhui Medical University |
And 2 more authors.
Korean Circulation Journal | Year: 2016
Background and Objectives: To explore the lung-protective effect of levosimendan (LS) during cardiopulmonary bypass in a canine model by determining the wet/dry weight (W/D) ratio of lung tissue, malonaldehyde (MDA) and superoxide dismutase (SOD) concentrations, and performing a histological evaluation. Materials and Methods: Thirty-two canines were divided randomly into four groups and underwent a routine aortic cross-clamping cardiopulmonary bypass procedure for 1 h, followed by recovery for 2 h. Animals were handled as follows: group C (means control group), no special treatment after aortic cross clamping; group P (means pulmonary artery perfusion group), pulmonary artery perfusion with cold oxygenated blood after aortic cross clamping; group LSIV (means intravenous injection of LS group), intravenous injection of LS (65 μg/kg) before thoracotomy, and the rest of the procedure was identical to the control group; group LPS (means pulmonary perfusion with LS group), pulmonary perfusion with cold oxygenated blood combined with LS (65 μg/kg) after aortic cross clamping. Lung tissues were removed and subjected to evaluation of pathological alterations, W/D ratio and MDA and SOD concentrations. Results: In group C, the W/D ratio and MDA concentration were higher, while the SOD concentrations were lower (p<0.05). Compared with groups P and LSIV, the MDA concentration was lower in group LPS, while that of SOD was higher (p<0.05); Light and electron microscopy indicated that LS intervention reduced impairment of lung tissues. Conclusion: Our findings suggest that LS plays an important role in protecting lung tissues. Copyright © 2016 The Korean Society of Cardiology.
Wang Q.,Anhui Medical University |
Wang Q.,Hefei Second Peoples Hospital |
Liang L.,Anhui Medical University |
Liu Y.,Anhui Medical University |
Zhang M.,University of Otago
Journal of Voice | Year: 2016
Objectives The cricoarytenoid joint has a loose capsule and large cavity and may allow the arytenoid distanced from the cricoid cartilage. The objective was to quantify vertical motion of the arytenoid cartilage in the living subject. Study Design This is a prospective study. Methods Axial computed tomography images from 35 healthy subjects and seven patients with unilateral vocal fold paralysis were collected at inspiration and phonation. The perpendicular distance from the arytenoid vocal process (VP) or muscular process (MP) to the cricoid plane was measured and analyzed. Results During phonation, the range of the vertical movement of the VP was significantly wider than that of the MP. The vertical motion varies in sides, sexes, and ages. The vertical gaps of the VP and MP between the paralyzed and contralateral sides were about 0.8 mm and 1.5 mm, respectively. Conclusions This study confirms a visor-like downward vertical motion of the arytenoid cartilage during phonation. © 2016 The Voice Foundation.
Shi Y.,Anhui Medical University |
Shi Y.,Hefei second peoples Hospital |
Shen G.,Anhui Province Key Laboratory of Molecular Medicine |
Fang H.,Hefei second peoples Hospital |
And 2 more authors.
Biomedical Research (India) | Year: 2015
To explore the method for quantitative determination of active constituent in Sophora alopecuroides L. and its anti-cancer activity. Method for quantitative determination of matrine in Sophora alopecuroides L. is established using HPLC with CLC-phenyl column, mobile phase of acetonitrile-anhydrous ethanol-3% phosphoric acid solution (80:10:10), detection wavelength of 220 nm and flow rate of 1.0 mL·min-1. Breast cancer MCF-7 cells are cultured by routine method. Inhibitory effect of matrine on breast cancer MCF-7 cell proliferation is determined by MTT assay. Flow cytometry is used to analyze the changes in cell cycle after treatment, and record percentages of Bax and Bcl-2 positive cells. 48 h after treatment with test concentrations of matrine, cell cycle of MCF-7 cells are evidently altered. With the addition of matrine, S phase MCF-7 cells are markedly reduced, and G0/G1 phase cells markedly increase, while G2/M phase cells do not change much. Flow cytometry results show that the test concentrations of matrine can effectively inhibit the viability of MCF-7 cells, and promote their apoptosis. Different concentrations of matrine can all somewhat increase the positive rate of Bax expression, and the effect exhibits an increasing trend with increasing concentration. Bcl-2 expressions of treatment groups are all evidently lower than the control group, showing a negative correlation. HPLC method is reliable and accurate in determining alkaloids in Sophora alopecuroides L., and matrine in Sophora alopecuroides L. can effectively inhibit the proliferation of breast cancer MCF-7 cells. © 2015 Scientific Publishers of India. All rights reserved.
PubMed | Hefei Second Peoples Hospital and Anhui Medical University
Type: | Journal: BMC public health | Year: 2015
To investigate the association between social capital and quality of life among type 2 diabetes patients in Anhui province, China.In a cross-sectional study, 436 adults with type 2 diabetes were interviewed. The two domains of Quality of life, physical component summary (PCS) and mental component summary (MCS), were measured using the Short-Form Health Survey (SF-36). A modified instrument scale was used to measure cognitive and structural social capital. Multiple logistic regression models were used to assess the associations between social capital and quality of life, adjusting for social economic status and risk factors for health.24.3 % of participants (106) were in poor PCS and 25.0 % (109) in poor MCS. The proportions of participants who had low cognitive and structural social capital were 47.0 % (205) and 64.4 % (281), respectively. Results of logistic regression models showed that cognitive social capital was positively associated with PCS (OR=1.84; 95 % CI: 1.12, 3.02) and MCS (OR=1.65; 95 % CI: 1.03, 2.66). However, the associations between structural social capital and PCS (OR=0.80, 95 % CI: 0.48, 1.34) and MCS (OR=0.62; 95 % CI: 0.38, 1.01) were not statistically significant.It is the first study in China to investigate associations between quality of life and social capital in type 2 diabetes. Findings document that cognitive social capital is associated with the quality of life of type 2 diabetes patients. Our study suggests that the social capital theory may provide a new approach to increase physical resources in diabetes prevention and control, especially in Low and Middle Income countries (LMICs).