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Feng J.,Anhui Medical University | Liu H.,Hefei Second Peoples Hospital | Chen J.,Anhui Medical University | Wang J.,Anhui Medical University | And 2 more authors.
Korean Circulation Journal | Year: 2016

Background and Objectives: To explore the lung-protective effect of levosimendan (LS) during cardiopulmonary bypass in a canine model by determining the wet/dry weight (W/D) ratio of lung tissue, malonaldehyde (MDA) and superoxide dismutase (SOD) concentrations, and performing a histological evaluation. Materials and Methods: Thirty-two canines were divided randomly into four groups and underwent a routine aortic cross-clamping cardiopulmonary bypass procedure for 1 h, followed by recovery for 2 h. Animals were handled as follows: group C (means control group), no special treatment after aortic cross clamping; group P (means pulmonary artery perfusion group), pulmonary artery perfusion with cold oxygenated blood after aortic cross clamping; group LSIV (means intravenous injection of LS group), intravenous injection of LS (65 μg/kg) before thoracotomy, and the rest of the procedure was identical to the control group; group LPS (means pulmonary perfusion with LS group), pulmonary perfusion with cold oxygenated blood combined with LS (65 μg/kg) after aortic cross clamping. Lung tissues were removed and subjected to evaluation of pathological alterations, W/D ratio and MDA and SOD concentrations. Results: In group C, the W/D ratio and MDA concentration were higher, while the SOD concentrations were lower (p<0.05). Compared with groups P and LSIV, the MDA concentration was lower in group LPS, while that of SOD was higher (p<0.05); Light and electron microscopy indicated that LS intervention reduced impairment of lung tissues. Conclusion: Our findings suggest that LS plays an important role in protecting lung tissues. Copyright © 2016 The Korean Society of Cardiology. Source

Mao C.,Southern Medical University | Qiu L.-X.,Fudan University | Zhan P.,Nanjing Chest Hospital | Xue K.,Fudan University | And 4 more authors.
Journal of Cancer Research and Clinical Oncology | Year: 2010

Purpose: Published data on the association between manganese superoxide dismutase (MnSOD) Val16Ala polymorphism and prostate cancer (PCA) risk are inconclusive. To derive a more precise estimate of the association between them, a meta-analysis was performed. Methods: PubMed and Embase were searched. All eligible studies were retrieved. The pooled odds ratio (OR) with 95% confidence interval (CI) for PCA risk associated with Val/Ala versus Val/Val, Ala/Ala versus Val/Val, dominant model (Ala/Ala + Val/Ala vs. Val/Val), and recessive model (Ala/Ala vs. Val/Ala + Val/Val) were estimated, respectively. Results: A total of 12 studies including 8,962 subjects were involved in this meta-analysis. Overall, the meta-analysis indicated that significantly elevated cancer risk was associated with Ala variant genotype when all the eligible studies were pooled into the meta-analysis (for Val/Ala vs. Val/Val: OR = 1.11, 95% CI = 1.00-1.24; for Ala/Ala vs. Val/Val: OR = 1.22, 95% CI = 1.00-1.49; for dominant model: OR = 1.14, 95% CI = 1.03-1.26). In the subgroup analysis by ethnicity, statistically significant increased risks were found among Caucasians with Ala allele (for Val/Ala vs. Val/Val: OR = 1.12, 95% CI = 1.00-1.25; for dominant model: OR = 1.14, 95% CI = 1.02-1.26). However, no significant associations were found in Africans. Conclusions: This meta-analysis suggests that the Ala allele of the MnSOD gene was a low-penetrance susceptible gene in PCA development, especially in Caucasians. © 2010 Springer-Verlag. Source

Mao C.,Southern Medical University | Qiu L.-X.,Fudan University | Liao R.-Y.,Southern Medical University | Du F.-B.,Hefei Second Peoples Hospital | And 4 more authors.
Lung Cancer | Year: 2010

Epidemiologic studies have evaluated the association between KRAS mutations and resistance to the treatment of epidermal growth factor receptor (EGFR) tyrosine-kinase inhibitors (TKIs) in patients with non-small cell lung cancer (NSCLC). However, results were inconclusive. To derive a more precise estimation of the relationship, we performed this meta-analysis. Systematic computerized searches of the PubMed and Medline databases (up to Jun 30, 2009) were performed. A total of 22 studies were included in the final meta-analysis, consisting of 1470 NSCLC patients, of whom 231 had KRAS mutations (16%). Current or former smokers had a higher frequency of KRAS mutations than never smokers (25% versus 6%; OR = 4.36; P< 0.01). Mutations were more common among adenocarcinoma than other histologies (26% versus 16%; OR = 1.98; P< 0.01). The objective response rate (ORR) of NSCLC patients with mutant KRAS was 3% (6/210), whereas the ORR of NSCLC patients with wild-type KRAS was 26% (287/1125). The overall pooled RR for ORR was 0.29 (95% CI: 0.18-0.47; P< 0.01). Subgroup analyses were conducted on the basis of ethnicity and study treatment, all the results were not materially altered and did not draw different conclusions, indicating that our results were robust. In summary, this meta-analysis suggests that KRAS mutations may represent negative predictive biomarkers for tumor response in NSCLC patients treated with EGFR-TKIs. However, due to a mutually exclusive relationship between KRAS and EGFR mutation and no difference in survival between KRAS mutant/. EGFR wild-type and KRAS wild-type/. EGFR wild-type NSCLC, the clinical usefulness of KRAS mutation as a selection marker for EGFR-TKIs sensitivity in NSCLC is limited. © 2009 Elsevier Ireland Ltd. Source

Shi Y.,Anhui Medical University | Shen G.,Anhui Province Key Laboratory of Molecular Medicine | Fang H.,Hefei Second Peoples Hospital | Xu C.,Hefei Second Peoples Hospital | Hu S.,Anhui Medical University
Biomedical Research (India) | Year: 2015

To explore the method for quantitative determination of active constituent in Sophora alopecuroides L. and its anti-cancer activity. Method for quantitative determination of matrine in Sophora alopecuroides L. is established using HPLC with CLC-phenyl column, mobile phase of acetonitrile-anhydrous ethanol-3% phosphoric acid solution (80:10:10), detection wavelength of 220 nm and flow rate of 1.0 mL·min-1. Breast cancer MCF-7 cells are cultured by routine method. Inhibitory effect of matrine on breast cancer MCF-7 cell proliferation is determined by MTT assay. Flow cytometry is used to analyze the changes in cell cycle after treatment, and record percentages of Bax and Bcl-2 positive cells. 48 h after treatment with test concentrations of matrine, cell cycle of MCF-7 cells are evidently altered. With the addition of matrine, S phase MCF-7 cells are markedly reduced, and G0/G1 phase cells markedly increase, while G2/M phase cells do not change much. Flow cytometry results show that the test concentrations of matrine can effectively inhibit the viability of MCF-7 cells, and promote their apoptosis. Different concentrations of matrine can all somewhat increase the positive rate of Bax expression, and the effect exhibits an increasing trend with increasing concentration. Bcl-2 expressions of treatment groups are all evidently lower than the control group, showing a negative correlation. HPLC method is reliable and accurate in determining alkaloids in Sophora alopecuroides L., and matrine in Sophora alopecuroides L. can effectively inhibit the proliferation of breast cancer MCF-7 cells. © 2015 Scientific Publishers of India. All rights reserved. Source

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