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Shijiazhuang, China

Yu H.,China Institute of Technology | Chen L.,Tianjin University | Geng H.,China Institute of Technology | Zhao Z.,Hebei Zhongrun Pharmaceutical Co.
Huagong Xuebao/CIESC Journal | Year: 2010

Coal granular activated carbon with particle size distribution from 0.27 mm to 1.70 mm was used as adsorbent in a fixed-bed to separate deacetyl cephalosporin C(DCPC) from the waste drainage. The adsorbed DCPC on the carbon was eluted with 8%(vol)ethanol aqueous solution, and the obtained eluate contained DCPC in a yield of 60%~65% with a purity higher than 85%. After being further condensed through nanofilter membrane, the eluate was allowed to pass through anion-exchange resin Amberlite IRA67 on which DCPC was adsorbed. Desorption with 0.5 mol·L-1 sodium acetate buffer solution (pH6.3), DCPC was obtained as sodium salt solution in a yield of 90%~95% with 96% purity and the concentration was higher than 30 g·L-1. DCPC sodium salt was isolated by crystallization through adding acetone to the eluate. The adsorption capacity of the used coal granular activated carbon could be restored by saturation with alkali solution containing isopropanol and then neutralization with acidic solution. © All Rights Reserved. © All Rights Reserved.

Zhang J.F.,Hebei University of Science and Technology | Liu X.,Hebei University of Science and Technology | Hou L.M.,Hebei University of Science and Technology | Liu Q.,Hebei Zhongrun Pharmaceutical Co.
Applied Mechanics and Materials | Year: 2014

7-amino-cephalsporanic acid (7-ACA) is the key intermediate of cephalosporin, which has a strong sensitization and explosion hazard. So it seriously affects the worker's health in the workshop, the environment safety out of the workshop and work safety. The drying workshop of 7-ACA at a pharmaceutical enterprise in Shijiazhuang is investigated and the production process is analyzed, the key places of dust leakage and the causes of dust generation are identified. Combined with the pollution and safety risk in drying workshop, the targeted control measures are taken from the process technology, ventilation system and dust collecting device, which effectively removed the adverse effects from 7-ACA leaking. The study provides a reference for the dust control in similar enterprises. © (2014) Trans Tech Publications, Switzerland.

He J.,Hebei Normal University | Xu R.,Hebei Normal University | Chen X.,Hebei Normal University | Jia K.,Hebei Normal University | And 2 more authors.
Acta Biochimica et Biophysica Sinica | Year: 2010

To reduce the immunogenicity of recombinant staphylokinase, structure-based mutagenesis of Glu80 residue in wild-type staphylokinase (wt-Sak) was rationally designed and carried out by a modified QuikChange site-directed mutagenesis. Sak mutants, including Sak(E80A) and Sak(E80S), were successfully expressed in E. coli DH5α as a soluble cytoplasmic proteins and accounted for more than 40 of the total cellular proteins. The expressed proteins were purified by a three-step chromatographic purification process. SDS-PAGE and HPLC analyses results indicated that the purified proteins were almost completely homogeneous and the purities of Sak mutants exceeded 97. Analysis of fibrinolytic activity revealed that substitution of E80 residue with serine and alanine resulted in slightly increased specific activities of Sak mutants. Investigation of the immunogenicity of Sak mutants showed that the amount of specific anti-Sak IgG antibodies elicited by Sak(E80A) and Sak(E80S) in BALB/c mice decreased ∼35 and 27, respectively compared with wt-Sak. The abilities of Sak mutants to stimulate proliferation of T cells from BALB/c mice and to bind mouse anti-Sak polyclonal serum were significantly lower than those of wt-Sak. These results suggested that substitution of Glu80 residue by alanine and serine successfully eliminated part of T- and B-cell epitope of Sak molecule. Our findings suggested that simultaneous elimination of T- and B-cell epitopes was a useful method to reduce the immunogenicity of wt-Sak molecule and provided a strategy for engineering safe Sak-based fibrinolytics for the clinical treatment of acute myocardial infarction.

Zhang J.,Hebei Zhongrun Pharmaceutical Co. | Zhang J.,Hebei Normal University | Wang J.-F.,Hebei Normal University | Zhang J.-J.,Hebei Normal University
Wuli Huaxue Xuebao/ Acta Physico - Chimica Sinica | Year: 2012

Three rare earth complexes of [Ln(5-Cl-2MOBA) 3phen] 2 (Ln=Nd(1), Eu(2), Ho(3); 5-Cl- 2MOBA: 5-chloro-2-methoxybenzoate; phen: 1,10-phenanthroline) were synthesized and characterized by elemental analysis, thermogravimetry-differential thermogravimetry-differential scanning calorimetry (TG-DTG-DSC), infrared (IR) spectra, ultraviolet (UV) spectra, and molar conductance techniques. The fluorescence spectra showed that complex (2) emitted a characteristic fluorescence of the Eu 3+ ions. The thermal properties are discussed by thermogravimetry - differential scanning calorimetry / Fourier transform infrared (TG-DSC/FTIR) techniques. And the three-dimensional IR accumulation spectra for the three complexes were also analyzed. © Editorial office of Acta Physico-Chimica Sinica.

Cheng J.-S.,Tianjin University | Zhao Y.,Tianjin University | Qiao B.,Tianjin University | Lu H.,Tianjin University | And 5 more authors.
Applied Biochemistry and Biotechnology | Year: 2016

The intracellular proteomes of the Penicillium chrysogenum throughout pilot and industrial processes were investigated by using 2-DE combined with MALDI-TOF-TOF MS, respectively. We detected a total of 223 spots corresponding to 154 proteins and 231 spots corresponding to 157 proteins throughout pilot and industrial processes, respectively. The levels of glyceraldehyde-3-phosphate dehydrogenase increased (5.1- and 2.5-fold) under the pilot process, while its levels were no significant changes under the industrial process at 140 and 170 h when compared with that at 2 h. The levels of isocitrate lyase and fumarate hydratase were increased significantly under the industrial process, while their levels had no obvious changes after 20 h of fermentation throughout the pilot process. These results indicate that there were remarkable differences in carbohydrate metabolism (including glycolysis, gluconeogenesis, pentose phosphate pathway, and tricarboxylic acid cycle) of P. chrysogenum during the pilot and industrial fermentations, which likely result in alterations of the primary metabolism and penicillin biosynthesis. Moreover, the differences in the levels of proteins involved in amino acid metabolisms (including valine, cysteine, and α-aminoadipic acid biosynthesis) indicated that the pilot and industrial processes influenced the supplies of penicillin precursors. Compared with that at 2 h, the maximum levels of superoxide (6.9-fold, at 32 h) and catalase (9-fold, at 80 h) during the industrial process and the maximum levels of superoxide (1.2-fold, at 20 h) and catalase (7.7-fold at 128 h) during the pilot process revealed the significant difference in cell redox homeostasis and stress responses during scale-up fermentation. Particularly, 10 spots corresponding to isopenicillin N synthetase and 4 spots corresponding to isopenicillin N (IPN) acyltransferase in pilot and industrial processes were identified, respectively. The levels of IPN acyltransferase (spots 197 and 198) and CoA ligase at 80 h during the industrial process were around 2-fold of that during the pilot process, indicating that the industrial process with a higher penicillin production per cell might provide available environments to induce over-expression of IPN acyltransferase and accelerate penicillin formation. These results provide new insights into the globally potential responses of P. chrysogenum to variations of environments in different fermentation scales so as to consequently regulate the penicillin production. © 2016 Springer Science+Business Media New York

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