Shijiazhuang, China
Shijiazhuang, China

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Chen F.Q.,Hebei Provincial Peoples Hospital | Yang Y.Z.,Hebei Provincial Peoples Hospital | Yu L.L.,Hebei Provincial Peoples Hospital | Bi C.B.,Hebei Provincial Peoples Hospital
Nigerian Journal of Clinical Practice | Year: 2015

Background: Atypical pneumonia caused by Mycoplasma pneumoniae is a leading cause of mortality among the pediatric age group. Objectives: Our study was designed to know the prevalence of M. pneumoniae in children with community-acquired pneumonia and the involvement in the cytoadherence to the respiratory epithelium by M. pneumoniae using electron microscopy and immuno-gold labeling technique. Materials and Methods: A total of 152 children of 1 month to 12 years of age of both sexes attending Hebei Provincial People's Hospital, Shijiazhuang, Hebei with diagnosed pneumonia were included in the study. Results: Out of 152 children 84 (55.3%) were males, and 68 (44.7%) were females. The mean age of the patients in the control group (50 patients) was 18.5 ± 3 months with 31 (62%) males and 19 (38%) females. IgM antibodies against M. pneumoniae were positive in 84 (55.3%) males and 68 (44.7%) females. Out of 50 patients 9 (18%) were found to positive for IgM M. pneumoniae antibodies of which four (44.4%) males and 5 (55.5%) females were positive. Our study observed that the gold particles were clustered on the filamentous extension of the tip of the cells. Out of 152 serum samples subjected to particle agglutination assay 138 (90.7%) were positive 1:320 titer, 9 were >1:80 and 3 showed titer was >1:40. Conclusion: We suggest that clinicians should consider empirical therapy of broad spectrum antibiotics therapy to cover these atypical pathogens to reduce the severity before obtaining the serological results. From our study, we also suggest electron microscopic and biochemical studies for better diagnosis of these pathogens.


Yaping Z.,Hebei Medical University | Ying W.,Hebei Provincial Peoples Hospital | Luqin D.,Hebei Medical University | Ning T.,Hebei Medical University | And 2 more authors.
APMIS | Year: 2014

Hepatic stellate cells (HSCs) are the major producers of collagen in the liver. Their conversion from resting cells to proliferative, contractile, and activated cells is a critical step leading to liver fibrosis that is characterized by the deposition of excessive extracellular matrix. Interleukin-1 (IL-1) may play a role in maintaining HSC in a proliferative state that is responsible for hepatic fibrogenesis. The aim of this study was to study the roles of the IL-1 type I receptor (IL-1R1), c-Jun N-terminal kinase (JNK), and activation protein-1 (AP-1) in IL-1β-mediated proliferation in rat HSCs. We showed that IL-1β can upregulate proliferation in rat HSCs; however, inhibition of the JNK pathway could inhibit HSCs proliferation. Furthermore, IL-1β activated IL-1R1 expression, the JNK signaling pathway, and AP-1 activity in a time-dependent manner in rat HSCs. These data demonstrate that IL-1β could promote the proliferation of rat HSCs and that the IL-1R1, JNK, and AP-1 pathways were involved in this process. In summary, IL-1β-induced proliferation is possibly mediated by the IL-1R1, JNK, and AP-1 pathways in rat HSCs. Therefore, drugs that block these pathways may inhibit the proliferation of HSCs and suppress liver fibrosis. © 2013 APMIS.


Wang D.,Hebei University of Engineering | Sun L.,Hebei University of Engineering | Song G.,Hebei Provincial Peoples Hospital | Chen S.,Hebei Provincial Peoples Hospital
International Journal of Clinical and Experimental Medicine | Year: 2015

Objective: This study was designed to evaluate the clinical efficacy of intensive insulin therapy for patients newly diagnosed with type 2 diabetes. Methods: A total of 219 patients newly diagnosed with type 2 diabetes were randomly assigned into insulin group (n = 55), gliclazide group (n = 52), metformin group (n = 55) and pioglitazone group (n = 57). On the basis of diet and physical interventions, patients in the insulin group received intensive insulin therapy. Those in other three groups were given oral intake of medication. All treatment schemes endured for 12 weeks. A variety of indexes including fasting blood-glucose (FPG), FPG at 2 h after diet (FPG 2 h), hemoglobin A1 c (HbAlc), area under the curve (AUC) for insulin (insulin AUC) after glucose load, C-peptide AUC after glucose load (C-peptide AUC), changes in insulin secretion index (Homa-β) and insulin resistance index(Homa-IR) were accurately measured and statistically among different groups. Results: The insulin AUC at 0-30 min, C-peptide AUC at 0-30 min and Homa-β in the insulin group were equally significantly higher compared with those levels in the other three groups. In addition, the level of Homa-IR in the insulin, metformin and pioglitazone groups were all significantly reduced compared with the values prior to respective treatment (all P < 0.05). Conclusion: Compared with oral administration of hypoglycemic drugs, intensive insulin therapy is able to better improve pancreatic β cell function and insulin resistance for newly-diagnosed type 2 diabetes patients. © 2015, E-Century Publishing Corporation. All rights reserved.


Zhou S.-H.,Hebei Provincial Peoples Hospital | Song Y.-B.,Hebei Provincial Peoples Hospital | Liu L.-J.,Hebei Provincial Peoples Hospital | Cui H.-S.,Hebei Provincial Peoples Hospital
World Journal of Surgical Oncology | Year: 2016

Background: Total thoracoscopic and laparoscopic esophagectomy (TLE) has attracted attention with the advantage of better operative field and minimal wound for the esophageal cancer. However, various severe complications are also reported during the TLE such as cervical anastomotic leakage, chylothorax, and tracheal injury. The aim of this study was to introduce a new optimized TLE procedure for the esophageal cancer and assess its safety and clinical effects. Methods: We retrospectively collected the clinical data of 30 patients with esophageal cancer who underwent optimized TLE procedures between January 2014 and December 2014. The optimized TLE procedures mainly include as follows: (1) 50 ml of sesame oil-milk mixture (1:1) is injected via gastric tube after endotracheal intubation; (2) patients are intubated with a single lumen endotracheal tube; (3) patients were positioned at 150° in the left prone position rather than lateral decubitus position; and (4) duodenal feeding tube was not placed intraoperatively and however triple lumen nasojejunal feeding tube was placed on the second postoperative day under imaging guidance. Operation time, amount of blood loss, number of dissected nodes, length of hospital stays, and complications were recorded. Results: The mean operation time of the optimized TLE group was 202.13 ± 13.74 min. The mean visible blood loss of the optimized TLE group was 300.00 ± 120.12 ml. The postoperative hospital stays in the optimized TLE group were 16.27 ± 4.51 days. The number of dissected nodes in the optimized TLE group was 13.57 ± 2.76. The postoperative complications for the optimized TLE procedure were seen in one case (3.3 %). Conclusions: The method of optimized TLE is an effective, reliable, and safe procedure for the treatment of esophageal cancer, which provide favorable outcomes in terms of operation time, blood loss, length of hospital stays, the number the dissected nodes, and reduced incidence of postoperative complications compared to previous literatures. Further studies with a large number of samples are warranted. © 2016 Zhou et al.


Li S.R.,Hebei Provincial Peoples Hospital
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2012

To study the effects of Naoxintong Capsule (NC) on the inflammation and long-term prognosis in the borderline lesion coronary heart disease patients. A total of 240 coronary heart disease patients with angina symptoms and accompanied with borderline lesion coronary heart disease (with the diameter stenosis in critical 50% -70%) by means of coronary angiography or multislice computed tomography coronary angiography were recruited. These patients were randomly assigned to the conventional treatment group (including nitrate, beta blockers, anti-platelet, anticoagulation, angiotensin converting enzyme inhibitors, and so on) and the NC treatment group (treated the same way as those for the conventional treatment group and NC). All patients were treated for 12 months. The occurrence of cardiovascular events was observed after treatment. The inflammatory factors in serum [interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-alpha), and high sensitive C reaction protein (hs-CRP)], matrix metalloproteinases-9 (MMP-9), blood lipids and blood sugar, liver and kidney functions were measured before and after treatment. After 12 months of treatment, the incidence of angina pectoris patients (6.67% vs 15.83%, P < 0.05) and hospitalization due to acute coronary syndrome (ACS) attacks (4.17% vs 10.83%) was significantly lower in the NC treatment group than in the conventional treatment group. There was no statistical difference in the serum levels of IL-6, TNF-alpha, hs-CRP, and MMP-9 between the two groups before treatment (P > 0.05). After 12 months of treatment, serum levels of IL-6, TNF-alpha, hs-CRP, and MMP-9 were significantly lower when compared with before treatment in the same group (P < 0.05). Besides, the serum levels of IL-6, TNF-alpha, hs-CRP, and MMP-9 were significantly lower in the NC group than in the conventional treatment group (P < 0.05). By means of Logistic regression analysis we found that the post-treatment MMP-9 level and IL-6 level were independent risk factors influencing the recurrence of angina pectoris. NC could alleviate the inflammation. Long-term administration of NC could reduce the recurrence of angina pectoris and decrease the incidence of ACS attack in borderline lesion coronary heart disease patients. The post-treatment MMP-9 level and IL-6 level were independent risk factors influencing the recurrence of angina pectoris.


Zhou J.,Hebei Provincial Peoples Hospital
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology | Year: 2013

This study was aimed to explore the killing effect of PBMNC induced by IL-23 alone or combined with IL-2 on K562 cells and its mechanism. The PBMNC were induced in vitro by IL-23 (50 ng/ml) alone or IL-23 combined with IL-2 (100 U/ml) for 72 h, and then were co-cultured with leukemia cell line K562. The CCK-8 method was used to detect the effect of PBMNC induced at different times on K562 cells, the ELISA was performed for detecting IFN-γ level in culture supernatant, and the perforin and granzymes B were detected by RQ-PCR. The results showed that the killing effect of PBMNC induced by IL-23 alone or IL-23 combined with IL-2 on K562 cells was observed, and obviously enhanced with prolonging of time, moreover, there was statistical difference among different time points (P < 0.05). The IFN-γ level in supernatant of PBMNC cultured with cytokines significantly increased, and the IFN-γ levels in group of IL-23 combined with IL-2 were higher than that in other groups (P < 0.05). The mRNA expressions level of perforin and granzymes B of the expanded PBMNC in groups cultured with cytokines were higher than that in control group (P < 0.05), and the mRNA expressions of perforin and granzymes B in group of IL-23 combined with IL-2 were significantly higher than that in others (P < 0.05). It is concluded that IL-23 can promote the killing effect of PBMNC on K562 cells. The combination of IL-2 with IL-23 displays synergic effect and a time-dependent manner. IL-23 also enhances the expression of IFN-γ, perforin and granzyme B in PBMNC. Its combination with IL-2 displays synergistic effect, suggesting that the anti-leukemic activity of IL-23 may be realized through inducing PBMNC to express IFN-γ, perforin and granzyme B.


Liu N.,Hebei Medical University | Li Y.,Hebei Provincial Peoples Hospital | Su S.,Hebei Medical University | Wang N.,Hebei Medical University | And 2 more authors.
Oncology Letters | Year: 2013

The Na+ /K+ -ATPase α subunit is highly expressed in malignant cells. Ouabain, a cardioactive glycoside, binds to the Na+ /K+ -ATPase α subunit and inhibits the activity of Na+ /K+ -ATPase. In the present study, the effect of ouabain on the migration of A549 cells was analyzed using the wound healing and transwell chamber migration assays. The impact of ouabain on the expression of E-cadherin, N-cadherin, vimentin, matrix metalloprotease (MMP)-2 and MMP-9 was also evaluated. Ouabain treatment not only inhibited the epidermal growth factor (EGF)-enhanced migration of A549 cells, but also inhibited the basal migration of A549 cells in the absence of EGF. Ouabain decreased the overexpression of N-cadherin and vimentin induced by EGF, and decreased the expression of MMP-2 and -9 in the presence or absence of EGF. Na+ /K+ -ATPase is a potent therapeutic target in lung cancer and these observations indicated that the Na+ /K+ -ATPase inhibitor, ouabain, retards the invasion of lung cancer cells.


Tang N.,Hebei Medical University | Zhang Y.-P.,Hebei Medical University | Ying W.,Hebei Provincial Peoples Hospital | Yao X.-X.,Hebei Medical University
Molecular Medicine Reports | Year: 2013

Matrix metalloproteinase-13 (MMP-13) is crucial in the cleavage and remodeling of the extracellular matrix (ECM), and its expression levels are decreased following the induction of liver fibrosis. The aim of the present study was to investigate the role of c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) in interleukin (IL)-1β-mediated MMP-13 gene expression in rat hepatic stellate cells (HSCs). In the present study, we demonstrated that IL-1β is capable of activating JNK and p38 in a time-dependent manner and the inhibition of the JNK pathway is able to increase MMP-13 mRNA expression; however, the inhibition of the p38 MAPK pathway is capable of inhibiting MMP-13 gene expression. These data demonstrate that IL-1β is able to promote MMP-13 mRNA expression in rat HSCs and the JNK and p38 MAPK pathways were involved in this process. In summary, IL-1β-induced MMP-13 mRNA expression is possibly mediated by cytoplasmic JNK and p38 MAPK pathways, and they play a distinct role in this process. Thus, the JNK and p38 MAPK pathway co-operatively mediate MMP-13 mRNA expression in rat HSCs.


PubMed | Hebei Provincial Peoples Hospital
Type: Journal Article | Journal: Nigerian journal of clinical practice | Year: 2015

Atypical pneumonia caused by Mycoplasma pneumoniae is a leading cause of mortality among the pediatric age group.Our study was designed to know the prevalence of M. pneumoniae in children with community-acquired pneumonia and the involvement in the cytoadherence to the respiratory epithelium by M. pneumoniae using electron microscopy and immuno-gold labeling technique.A total of 152 children of 1 month to 12 years of age of both sexes attending Hebei Provincial Peoples Hospital, Shijiazhuang, Hebei with diagnosed pneumonia were included in the study.Out of 152 children 84 (55.3%) were males, and 68 (44.7%) were females. The mean age of the patients in the control group (50 patients) was 18.5 3 months with 31 (62%) males and 19 (38%) females. IgM antibodies against M. pneumoniae were positive in 84 (55.3%) males and 68 (44.7%) females. Out of 50 patients 9 (18%) were found to positive for IgM M. pneumoniae antibodies of which four (44.4%) males and 5 (55.5%) females were positive. Our study observed that the gold particles were clustered on the filamentous extension of the tip of the cells. Out of 152 serum samples subjected to particle agglutination assay 138 (90.7%) were positive 1:320 titer, 9 were >1:80 and 3 showed titer was >1:40.We suggest that clinicians should consider empirical therapy of broad spectrum antibiotics therapy to cover these atypical pathogens to reduce the severity before obtaining the serological results. From our study, we also suggest electron microscopic and biochemical studies for better diagnosis of these pathogens.


PubMed | Hebei Provincial Peoples Hospital
Type: | Journal: World journal of surgical oncology | Year: 2016

Total thoracoscopic and laparoscopic esophagectomy (TLE) has attracted attention with the advantage of better operative field and minimal wound for the esophageal cancer. However, various severe complications are also reported during the TLE such as cervical anastomotic leakage, chylothorax, and tracheal injury. The aim of this study was to introduce a new optimized TLE procedure for the esophageal cancer and assess its safety and clinical effects.We retrospectively collected the clinical data of 30 patients with esophageal cancer who underwent optimized TLE procedures between January 2014 and December 2014. The optimized TLE procedures mainly include as follows: (1) 50 ml of sesame oil-milk mixture (1:1) is injected via gastric tube after endotracheal intubation; (2) patients are intubated with a single lumen endotracheal tube; (3) patients were positioned at 150 in the left prone position rather than lateral decubitus position; and (4) duodenal feeding tube was not placed intraoperatively and however triple lumen nasojejunal feeding tube was placed on the second postoperative day under imaging guidance. Operation time, amount of blood loss, number of dissected nodes, length of hospital stays, and complications were recorded.The mean operation time of the optimized TLE group was 202.1313.74 min. The mean visible blood loss of the optimized TLE group was 300.00120.12 ml. The postoperative hospital stays in the optimized TLE group were 16.274.51 days. The number of dissected nodes in the optimized TLE group was 13.572.76. The postoperative complications for the optimized TLE procedure were seen in one case (3.3%).The method of optimized TLE is an effective, reliable, and safe procedure for the treatment of esophageal cancer, which provide favorable outcomes in terms of operation time, blood loss, length of hospital stays, the number the dissected nodes, and reduced incidence of postoperative complications compared to previous literatures. Further studies with a large number of samples are warranted.

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