Hebei Provincial Key Laboratory of Tree Species Germplasm Resources and Forest Protection

Baoding, China

Hebei Provincial Key Laboratory of Tree Species Germplasm Resources and Forest Protection

Baoding, China
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Zhao X.,Agricultural University of Hebei | Zhao X.,Hebei Provincial Key Laboratory of Tree Species Germplasm Resources and Forest Protection | Liu M.,Agricultural University of Hebei | Liu M.,Hebei Provincial Key Laboratory of Tree Species Germplasm Resources and Forest Protection | And 8 more authors.
Polish Journal of Environmental Studies | Year: 2017

Three strains, designated A1, B2, and C3, capable of aerobic degradation of 4-monobrominated diphenyl ether (4-BDE) as sole carbon and energy source were originally isolated from Populus tomentosa phyllosphere. The three strains were identified as Sphingomonas, Enterobacteria, and Curtobacterium based on their morphology and molecular properties. The 4-BDE concentration gradient was set up and showed that the three strains could still survive at 500 mg L-1 4-BDE. Their removal efficiencies were detected by utilizing GC-μECD and the analysis showed that A1, B2, and C3 were able to transform 33%, 20%, and 17% of 80 mg L-1 4-BDE in 2d incubation, respectively. This is the first report of aerobic degradation of 4-BDE by three bacterial strains isolated from phyllosphere. © 2017, HARD Publishing Company. All rights reserved.


Cai M.,Agricultural University of Hebei | Cai M.,Hebei Provincial Key Laboratory of Tree Species Germplasm Resources and Forest Protection | Li Y.,Agricultural University of Hebei | Li Y.,Hebei Provincial Key Laboratory of Tree Species Germplasm Resources and Forest Protection | And 2 more authors.
Environmental Science and Pollution Research | Year: 2015

Populus species are very effective in remediation of contaminants. Polybrominated diphenyl ethers (PBDEs) are commonly used as flame retardants and are known to be persistent environmental pollutants. Numerous studies have shown that PBDEs are rising in human tissues and biota. 4-Monobrominated diphenyl ether (4-BDE), one of the less brominated PBDEs, was served as a model compound for biodegradation of lower brominated congeners. The present study was designed to clarify the effects of 4-BDE stress on morphological, physiological, and biochemical impacts of Populus tomentosa Carr in a tissue culture condition. Different concentrations of 4-BDE (3 and 30 mg L−1) were supplied alone or together with 0.5 mg L−1 IBA in tissue culture media. With the concentration increased, 4-BDE caused negative effects on the microscopic structure of roots, stem, and leaves. The leaf color became shallow in low concentration of 4-BDE treatments and appeared albinism with 4-BDE concentration increased. The chlorophyll content and the leaf mass per area of albino leaves reduced significantly. 4-BDE also caused positive effects on the adventitious root differentiation and the biomass below 30 mg L−1. With the 4-BDE treatment time increased (23, 47, and 58 days), the peroxidase (POD) activity displayed the decreasing trend. The proline content decreased first and then increased. Exposure to 4-BDE induced the malondialdehyde (MDA) to increase in leaves. Application of 4-BDE affected the endogenous hormone levels of cuttings in their adventitious roots inducing media. Below 0.3 mg L−1, 4-BDE caused the faint expression of auxin-sensitive DR5::GUS reporter gene in Arabidopsis thaliana. Additionally, P. tomentosa Carr exhibited the better tolerance against 4-BDE in the range of less than 30 mg L−1. © 2015, Springer-Verlag Berlin Heidelberg.


Song G.,Agricultural University of Hebei | Song G.,Hebei Provincial Key Laboratory of Tree Species Germplasm Resources and Forest Protection | Cai M.,Agricultural University of Hebei | Cai M.,Hebei Provincial Key Laboratory of Tree Species Germplasm Resources and Forest Protection | And 2 more authors.
Chinese Journal of Ecology | Year: 2016

A total of 12 different endophytic bacterial strains were isolated from Salix matsudana f. pendula in garden and its tissue culture seedlings in this study. Endophytic bacterial strain CGL-1 was identified by using Aroclor 1242 as the only carbon source to grow well. The characteristics of 16S ribosomal DNA sequences and morphology of colony and cell suggested that this isolate was most closely related to the genus Enterobacter. The optimal conditions of Aroclor 1242 degradation were obtained according to the changes of inoculation amount of the bacterium, pH and the concentration of NaCl in liquid minimal media. The inoculation amount of 10% of CGL-1 at pH 7.0 appeared to be the optimum conditions for removing Aroclor 1242. Under these conditions, GC-ECD was used to measure removal effects of Aroclor 1242 by domesticated strain CGL-1. The results indicated that Aroclor 1242 congeners were obviously removed by strain CGL-1, with a total removal rate of 43.2% after 7 d. © 2016, Editorial Board of Chinese Journal of Ecology. All Rights Reserved.

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