Key Laboratory of Pharmaceutical Quality Control of Hebei Province

Laboratory of, China

Key Laboratory of Pharmaceutical Quality Control of Hebei Province

Laboratory of, China

Time filter

Source Type

Yang G.,Key Laboratory of Pharmaceutical Quality Control of Hebei Province | Yang G.,Hebei University | Yan C.,Key Laboratory of Pharmaceutical Quality Control of Hebei Province | Yan C.,Hebei University | And 6 more authors.
Analytical Methods | Year: 2012

A strong cation exchange monolithic column was prepared by a single step in situ radical polymerization without further chemical modification for high performance liquid chromatography (HPLC). The novel monolithic column used vinyl ester resin as the monomer, ethylene glycol dimethacrylate (EDMA) as the cross linking agent, 2,2′-azobisisobutyronitrile (AIBN) as the initiating agent, NaHSO 3 both as inorganic adjunct and coadunate initiator (L. G. Bai, H. Y. Liu, Y. K. Liu, J. Chromatogr. A, 2010, 1218, 100-106, and F. A. Zhang, C. L. Yu, X. H. Zhu, C. Wei, Polym. Adv. Technol., 2006, 17, 608-611). Moreover, NaHSO 3 can supply sulfonic acid groups as cation exchangers for ion-exchange monolithic column. Polymerization conditions and separation conditions were optimized, and the structure properties of the monoliths were also investigated. In addition, the chromatographic performance of the monolith was evaluated through separating human serum albumin (HSA) from human plasma in conjunction with HPLC and the expected results were acquired. The effects of buffer concentration and pH on the separation of HSA were investigated. Furthermore, the monolith was used to separate the mixture of protein (hemoglobin and HSA) with good resolution. © 2012 The Royal Society of Chemistry.


Zhong J.,Key Laboratory of Pharmaceutical Quality Control of Hebei Province | Zhong J.,Hebei University | Hao M.,Key Laboratory of Pharmaceutical Quality Control of Hebei Province | Hao M.,Hebei University | And 6 more authors.
Journal of Chromatography A | Year: 2014

A new polymeric monolith was prepared in stainless-steel column and fused-silica capillary, respectively, by atom transfer radical polymerization technique. In the polymerization, triallyl isocyanurate (TAIC) was used as the functional monomer; trimethylolpropane triacrylate (TMPTA) as the crosslinking agent; polyethylene glycol 200 and 1,2-propanediol as the co-porogens; carbon tetrachloride as the initiator and ferrous chloride as the catalyst. The conditions of polymerization were optimized. Morphology of the prepared poly(TAIC-co-TMPTA) monolith was investigated by scanning electron microscopy; pore properties were assayed by mercury porosimetry and nitrogen adsorption. The characterization indicated that the prepared reversed-phase monolith possessed uniform structure, good permeability and mechanical stability. The column was used as the stationary phase of reversed phase high performance liquid chromatography (RP-HPLC) and capillary liquid chromatography (CLC) to separate the mixture of aromatic compounds. The new column performed around 125,000 theoretical plates per meter. The column showed good reproducibility: the relative standard deviation values of the retention factor values for aromatic compounds were less than 1.52% (n = 7, column-to-column). © 2014 Elsevier B.V.


Li R.,Key Laboratory of Pharmaceutical Quality Control of Hebei Province | Li R.,Hebei University | Zhong J.,Key Laboratory of Pharmaceutical Quality Control of Hebei Province | Zhong J.,Hebei University | And 6 more authors.
Analytical Methods | Year: 2014

A porous functional polymer was prepared by in situ polymerization reaction. In this process, azobisisobutyronitrile was used as the initiator; hexanediol diacrylate (HDDA) and butyl methacrylate (BMA) as co-monomers; dodecyl alcohol as the porogenic solvent. The conditions of polymerization were optimized. The morphology of the polymer was investigated by scanning electron microscopy. Chemical groups in the monolith were assayed by infrared spectrometry. The pore size distribution was determined by mercury intrusion porosimetry and nitrogen adsorption-desorption. Finally, the functional polymer, a poly(HDDA-co-BMA-co-EDMA) monolith, was used as the stationary phase of high performance liquid chromatography (HPLC) to separate small molecules. This journal is © 2014 The Royal Society of Chemistry.


Qin J.,Key Laboratory of Pharmaceutical Quality Control of Hebei Province | Qin J.,Hebei University | Bai L.,Key Laboratory of Pharmaceutical Quality Control of Hebei Province | Bai L.,Hebei University | And 9 more authors.
Analytical Methods | Year: 2015

A novel skeleton porous polymer-based monolith chromatography column has successfully been prepared using an in situ free radical polymerization technique. A 50 mm × 4.6 mm i.d. stainless steel chromatographic column used dodecanol as porogen and ionic liquid (IL), 1-dodecene (C12) and trimethylol propane triacrylate (TMPTA) as monomers, and ethylene dimethacrylate as crosslinker. The effect of variables such as temperature and porogen solvent content on the porous structure was studied in detail. The polymer-based monolith obtained was characterized by scanning electron microscopy, infrared spectroscopy, mercury intrusion porosimetry, and nitrogen adsorption. The results indicated that the monolithic column had a porous structure, good mechanical stability, high permeability (6.77 × 10-14 m2), and a high specific surface area (155.62 m2 g-1). The liquid chromatographic performance of the monolith was evaluated in the separation of lysozyme from egg white and in the separation of a variety of mixtures of small molecules, such as amines and benzene analogues. The column showed good repeatability and reproducibility, and the column-to-column (n = 7) and batch-to-batch (n = 5) reproducibility was 2.85 and 3.15%, respectively. © 2014 The Royal Society of Chemistry.


Guo H.,Hebei University | Guo H.,Key Laboratory of Pharmaceutical Quality Control of Hebei Province | Wu F.,Hebei University | Zhang W.,Hebei University | Liu F.,Hebei University
Chinese Journal of Chromatography (Se Pu) | Year: 2013

The structure of polysaccharide in traditional Chinese medicine (TCM) is complex and characteristic. A method of oligosaccharide electrophoresis analysis assisted with cluster analysis (CA) was established to simultaneously identify TCMs. Six TCMs from three families were selected as experimental subjects and their polysaccharides were extracted. The obtained oligosaccharides via incompletely degraded TCM polysaccharides were derivatized using 1 -phen-yl-3-methyl-5-pyrazolone (PMP). The PMP- oligosaccharide derivatives were separated and analyzed by capillary zone electrophoresis (CZE). The six TCMs were identified by the featured information of oligosaccharide electropherograms with CA. The electrophoresis conditions were as follows: uncoated fused silica capillary column (49 cm/40 cm (total length/effective length) x 50 jjim); running buffer solution, 50 mmol/L phosphate buffer solution (pH 2.5); detection wavelength, 245 nm; operating voltage, 15 kV; hydrodynamic pressure injection, 10 cm ×4 s. The results showed that the six TCMs from three families were effectively identified by the method of TCM oligosaccharide electropherograms combined with CA. This method is a promising tool to identify TCM with good reliability and reproducibility.


Guo H.,Hebei University | Guo H.,Key Laboratory of Pharmaceutical Quality Control of Hebei Province | Pang X.,Hebei University | Zhang W.,Hebei University | And 2 more authors.
Analytical Methods | Year: 2013

The dissolution determination of multiple components in herbal preparations is a crucial method for controlling the quality of oral solid dosage forms. In order to improve the low detection sensitivity of dissolution samples, an efficient method for the extraction and concentration of multiple components in dissolution samples of Huoxiang Zhengqi tablets was developed by partitioned dispersive liquid-liquid microextraction (PDLLME). In the PDLLME process, 1.6 mL of THF as the disperser solvent and 100 μL of 1,2-dichloroethane as the extraction solvent were injected into 8 mL of the dissolution sample solution. Based on the partition coefficient, a portion of THF will transfer into the extraction solvent, then the multiple components in the Huoxiang Zhengqi tablet can be extracted into the mixture extraction phase of 1,2-dichloroethane and partitioned THF. In combination with HPLC-UV analysis, the enrichment factors reached to 43 to 119-fold for five of the target components (three of the more polar components were unknown). For honokiol and magnolol, a linear range from 10 to 1000 μg L-1 was obtained, and the recoveries at three spiking levels ranged from 90.2 to 99.4% with RSD less than 7.2% (n = 3). The proposed method may be applied for the extraction and concentration of multiple components in the dissolution samples of other herbal preparations. © 2013 The Royal Society of Chemistry.


Bai L.,Key Laboratory of Pharmaceutical Quality Control of Hebei Province | Bai L.,Hebei University | Liu H.,Key Laboratory of Pharmaceutical Quality Control of Hebei Province | Liu H.,Hebei University | And 8 more authors.
Journal of Chromatography A | Year: 2011

A novel hybrid organic-inorganic monolith for high performance liquid chromatography (HPLC) was firstly developed by atom transfer radical polymerization (ATRP) by a simple and rapid method, in which vinyl ester resin was used as the monomer, natrium bisulfurosum was used both as organic adjunct and coadunate initiator to alter the activity of the free radical in the process of polymerization and then to control the molecular mass. The conditions of polymerization were optimized. The chemical group of the monolith was assayed by infrared spectra method, the morphology of monolithic material was studied by scanning electron microscopy (SEM) and the pore size distribution was determined by a mercury porosimeter. Finally, the monolith was used to separate lysozyme (Lys) from chicken egg white with good resolution and reproducibility that were obtained in a short time (10. min) by HPLC. In addition, the influences of buffer concentration and pH value on elution have been investigated and the hybrid monolith was used to separate benzene and its homologs from the mixture. © 2010 Elsevier B.V.


Yan H.,Hebei University | Wang F.,Hebei University | Wang H.,Hebei University | Yang G.,Key Laboratory of Pharmaceutical Quality Control of Hebei Province
Journal of Chromatography A | Year: 2012

A simple, convenient and high selective miniaturized molecularly imprinted matrix solid-phase dispersion (mini-MI-MSPD) technique using l-tryptophan-imprinted polymers as sorbents was proposed for simultaneous extraction of four auxins (indole-3-acetic acid (IAA), indole-3-propionic acid (IPA), indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA)) from orange samples coupled with high performance liquid chromatography-ultraviolet detection. The molecularly imprinted polymers (MIPs) synthesized using l-tryptophan as dummy template showed high affinity and selectivity to auxins, and so they were applied as the specific dispersant of MSPD to simultaneously eliminate the effect of template leakage on quantitative analysis. Under the optimized conditions, good linearity was obtained in a range of 0.02-2.00μgg-1 and the average recoveries of four auxins at three spiked levels ranged from 87.6% to 104.5% with the relative standard deviation (RSD) ≤4.4%. The presented mini-MI-MSPD method combined the advantages of MSPD for allowing the extraction, dispersion and homogenization in a single step and the advantages of MIPs for high affinity and selectivity towards four auxins, which could be applied to the determination of auxins in complicated vegetal samples. © 2012 Elsevier B.V.


Chen B.-H.,Hebei University | Chen B.-H.,Key Laboratory of Pharmaceutical Quality Control of Hebei Province | Li J.-T.,Hebei University | Chen G.-F.,Hebei University | And 2 more authors.
Letters in Organic Chemistry | Year: 2012

Ultrasound-promoted synthesis of β-acetamido ketones catalyzed by cobalt sulfate heptahydrate via one-pot multi-component reaction coupling of aromatic aldehyde, acetophenone and acetyl chloride in acetonitrile was carried out in excellent yields at 25-28 °C, providing an efficient synthesis of these compounds. The use of ultrasound increased the rate of reactions compared with reactions under reflux condition. © 2012 Bentham Science Publishers.


Zhang X.,Hebei University | Chen J.,Hebei University | Mao M.,Hebei University | Guo H.,Hebei University | And 2 more authors.
International Journal of Biological Macromolecules | Year: 2014

The central composite design (CCD) was applied to optimize the water extraction of the polysaccharide from Adenophorae Radix in the paper. The three variables of extraction temperature (X1), extraction time (X2) and ratio of water to raw material (X3) were investigated by single factor analysis first. Since the presence of active polysaccharides and the imperfect of its extraction, the purpose of the paper was to evaluate the effects of selected variables on the yield of polysaccharide, which was expected to obtain the maximum yield. By variance and regression analysis, the quadratic regression equation was established as a predicted model. The R2 of 0.9825 indicated that the equation was well-fitted. The optimal conditions were 72.5°C, 133min, 1:35 (g/mL) and the predicted maximum yield of the polysaccharide was 5.78%. The predicted value was verified in triplicates under the optimum conditions, which was 5.68% and well matched with the predictive yield. © 2014 Elsevier B.V.

Loading Key Laboratory of Pharmaceutical Quality Control of Hebei Province collaborators
Loading Key Laboratory of Pharmaceutical Quality Control of Hebei Province collaborators