Hebei Province Engineering Laboratory for Plant Breeding

Shijiazhuang, China

Hebei Province Engineering Laboratory for Plant Breeding

Shijiazhuang, China
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Xu J.,CAS Institute of Genetics and Developmental Biology | Xu J.,Hebei Province Engineering Laboratory for Plant Breeding | Zhu Y.,Nanjing Agricultural University | Ge Q.,CAS Institute of Genetics and Developmental Biology | And 8 more authors.
New Phytologist | Year: 2012

• Under cadmium (Cd) stress, Solanum nigrum accumulated threefold more Cd in its leaves and was tolerant to Cd, whereas its low Cd-accumulating relative, Solanum torvum, suffered reduced growth and marked oxidative damage. However, the physiological mechanisms that are responsible for differential Cd accumulation and tolerance between the two Solanum species are largely unknown. • Here, the involvement of antioxidative capacity and the accumulation of organic and amino acids in response to Cd stress in the two Solanum species were assessed. • Solanum nigrum contains higher antioxidative capacity than does S. torvum under Cd toxicity. Metabolomics analysis indicated that Cd treatment also markedly increased the production of several organic and amino acids in S. nigrum. Pretreatment with proline and histidine increased Cd accumulation; moreover, pretreatment with citric acid increased Cd accumulation in leaves but decreased Cd accumulation in roots, which indicates that its biosynthesis could be linked to Cd long-distance transport and accumulation in leaves. • Our data provide novel metabolite evidence regarding the enhancement of citric acid and amino acid biosynthesis in Cd-treated S. nigrum, support the role of these metabolites in improving Cd tolerance and accumulation, and may help to provide a better understanding of stress adaptation in other Solanum species. © 2012 The Authors New Phytologist © 2012 New Phytologist Trust.


Xu J.,CAS Institute of Genetics and Developmental Biology | Xu J.,Hebei Province Engineering Laboratory for Plant Breeding | Sun J.,CAS Institute of Genetics and Developmental Biology | Du L.,CAS Institute of Genetics and Developmental Biology | And 2 more authors.
New Phytologist | Year: 2012

• Solanum nigrum is a cadmium (Cd) accumulator, whereas Solanum torvum is a low Cd-accumulating plant. The molecular mechanisms that are responsible for differential cadmium (Cd) accumulation in the two Solanum species are poorly understood. • Here, grafting experiments confirmed that increased Cd loading into the root xylem was responsible for the differential Cd accumulation in the two Solanum species. An iron (Fe) supply assay indicated that low Fe accumulation in S. torvum leaves is related to its Cd sensitivity. • Transcriptome analyses revealed higher expression of the genes that encode several metal transporters as well as antioxidant-related genes, and several organic and amino acid biosynthesis/metabolism-related genes in Cd-treated S. nigrum. Our data also indicated that the different responsive mechanisms of the transporter genes to Fe deficiency might be responsible for differential uptake and redistribution of metals in the two Solanum species • These results form a basis upon which to further explore the molecular mechanisms of Cd accumulation and tolerance, and provide an insight into novel strategies that can be used for phytoremediation and food safety. © 2012 The Authors New Phytologist © 2012 New Phytologist Trust.

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