Peroxynitrite-induced expression of inducible nitric oxide synthase and activated apoptosis via nuclear factor-kappa B pathway in retinal pigment epithelial cells and antagonism of cholecystokinin octapeptide-8 in vitro
Hao L.-N.,Peoples Hospital of Hebei Province |
Zhang X.-D.,Hebei Province Peoples Hospital |
Wang M.,Peoples Hospital of Hebei Province |
Yang T.,Hebei Medical University |
He S.-Z.,301 Hospital
International Journal of Ophthalmology | Year: 2011
AIM: To explore that if peroxy nitrite induced the expression of inducible nitric oxide synthase (iNOS) via nuclear factor-kappa B (NF-κB) pathway in retinal pigment epithelial (RPE) cells and the antagonism of cholecy stokinin octapeptide-8 (Melatonin, CCK-8) in vitro. METHODS: RPE cells were obtained from eyes of C57 BL/6 mouse and divided into control, peroxy nitrite and CCK-8 groups. Control group was treated with saline, peroxy nitrite group was treated with peroxy nitrite, and CCK-8 group was treated with CCK-8 after added with peroxy nitrite. All changes were observered at 6, 12 and 24 hours after treatment. Gene array analysis, Reverse Transcription Poly merase Chain Reaction (RT-PCR) were used to determ ine the expression of inducible nitric oxide synthase (iNOS) mRNA in RPE cells. Western blotting was used to test the apoptosis of RPE cells. Immunofluorescent staining was used to determ ine the NF-κB pathway signal transduction. RESULTS: Compared to the control group, the expression of iNOS mRNA was up-regulated in peroxy nitrite group and down-regulated in CCK-8 group with gene array analysis. Apoptosis was increased in peroxy nitrite group and decreased in CCK-8 group with western blotting. The NF-κB pathway signal transduction was more and more stronger in the peroxy nitrite group. But in CCK-8 group, little stronger could be observed at 12 hours, then weak at 24 hours with immunofluorescent staining (P<0.001). CONCLUSION: This study suggested that apoptosis of RPE cells was partly induced by peroxy nitrite, which may be the new way of oxidative damage to the RPE cells. The NF-κB signal transduction may affect and reinforce apoptosis mediated by peroxy nitrite. CCK-8 decreased apoptosis of RPE cells induced by peroxy nitrite and is a potential agent for therapy of retinopathy. The mechanism of CCK-8 dealing with RPE cells may be related to its direct inhibition of the form ation of iNOS to produce peroxy nitrite and antagnism of damage of peroxy nitrite to the RPE cells. Copyright International Journal of Ophthalmology Press.
Bai W.,Hebei Medical University |
Liu N.,Hebei Province Peoples Hospital |
Bai Y.,Hebei Medical University
Chinese Journal of Gastroenterology | Year: 2013
In recent years, both domestic and international basic and clinical studies have shown that there is a close relationship between gut microflora and liver disease. This article focused on the close relationship between gut microflora and liver disease, stating what is the impact on adjustment of gut microflora by liver disease, and what is the effect of adjustment of microflora imbalance on liver disease. Objective evaluation of basic microflora and clinical research in liver disease could draw our attention to the relationship between gut microflora and liver disease, guiding the clinical application of probiotics for prolonging the survival time of patients with liver disease and improving the prognosis.
Cheng Y.,Hebei Province Peoples Hospital
Wei sheng yan jiu = Journal of hygiene research | Year: 2012
To explore the relationship of Ghrelin, insulin-like growth factor-1 (IGF-1) and insulin with the growth and development of 2 -7 year-old children with small for gestational age (SGA) at birth. The levels of ghrelin, IGF-1, IGFBP-3, insulin and glucose were measured in the children with preterm SGA and term SGA and compared with the children with preterm appropriate for gestational age (AGA) and term AGA. The correlation of ghrelin with IGF-1, IGFBP-3 and insulin was analyzed. Plasma ghrelin in preterm SGA was higher than that in term SGA (P < 0.05), and there was no significant difference between preterm SGA and preterm AGA (P > 0.05). Plasma ghrelin in preterm AGA and term SGA was higher than that in term AGA (P < 0.05, P < 0.01 respectively). Serum IGF-1 and IGFBP-3 in preterm SGA were lower than those in term SGA (P < 0.05 for all) and serum IGF-1 and IGFBP-3 in preterm AGA were much lower than those in term AGA (P < 0.0001 for all). The level of serum insulin was the highest in term SGA. The trend of insulin resistance index (IRI) was similar to insulin. There were negative correlations of ghrelin with other indexes (weight SDS, IGF-1, IGFBP-3, insulin and IRI) in preterm SGA and term SGA (in preterm SGA r = -0.683, P < 0.002; r = -0.749, P < 0.001; r = -0.828, P < 0.001; r = -0.694, P < 0.005; r = -0.822, P < 0.001; in term SGA r = -0.792, P < 0.001; r = -0.707, P < 0.002; r = -0.615, P < 0.01; r = -0.648, P < 0.005; r = -0.679, P < 0.005). Ghrelin is involved in the regulation of growth and development of preterm and SGA children, regardless of the magnitude of their catch up growth. As a re-regulatory factor to insulin, ghrelin regulates the energy metabolism in a form of negative feedback.
Xiao L.,Cangzhou City Central Hospital |
Zhang Y.,Cangzhou City Central Hospital |
Yang M.,Cangzhou City Central Hospital |
Zhou J.,Cangzhou City Central Hospital |
And 3 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2016
Objective: To investigate the clinical effect of minimally invasive direct coronary artery bypass (MIDCAB) in left chest with percutaneous coronary intervention (PCI) in the treatment of coronary heart disease. Methods: 100 cases of patients with coronary heart disease were randomly divided into observation group (50 cases) and control group (50 cases). The patients in observation group firstly underwent MIDCAB, and then underwent selective PCI to treat right coronary artery and vascular disease; the control group underwent traditional CABG. Indicators such as preoperative basic condition, postoperative drainage volume in 24 h, mechanical ventilation time, ICU monitoring time, hospital days, re-exploration for mediastinal bleeding, peri-operative myocardiac infarction and fatality rate etc. were compared between two groups. The occurrence of cardiovascular adverse events during follow-up period was also observed. Results: There were no significant differences of age, sex, history of diabetes and hypertension, the proportion of dyslipdemia, smoking, drinking, angina pectoris, and cardiac functional (NHYA classification) in the two groups of patients (P>0.05). Patients were followed-up for 3 months after operation; we found that the LVEF in observation group was obviously higher than that in control group and the difference had statistical significance (P<0.05). Compared with control group, the postoperative drainage volume in 24 h, mechanical ventilation time, CICU monitoring time, postoperative hospital stay, operative time and the length of incision were significantly less in observation group, which had statistical difference (P<0.05). There was no re-exploration for mediastinal bleeding or death in two groups. There was no significant difference in pleural effusion and infection between the two groups (P>0.05). The results of one-year follow-up showed that there were 2 cases of recurrence of myocardial infarction in the control group; however, there was no cardiac death or recurrence of myocardial infarction, or second CABG/PCI in observation group. Conclusion: For suitable patients with coronary heart disease, MIDCAB with PCI surgery is safe and reliable, and has a significant short-term clinical effect. © 2016, E-Century Publishing Corporation. All rights reserved.
Chen W.,Hebei Medical University |
Li X.,Hebei Province Peoples Hospital |
Su Y.,Hebei Medical University |
Zhang Q.,Hebei Medical University |
And 3 more authors.
Foot and Ankle International | Year: 2011
Background: Lateral hindfoot pain after union of a calcaneal fracture remains a challenge to orthopaedic surgeons. This study aimed to investigate the relationship between lateral calcaneal pain, calcaneal width and peroneal tendon sheath impingement in patients with lateral hindfoot pain. Materials and Methods: Seventy-four consecutive patients with unilateral lateral hindfoot pain were identified from an institutional trauma registry. Regular followups were performed and the severity of lateral hindfoot pain was recorded. CT scans were conducted to measure calcaneal width at the level of sustentaculum tali. Bilateral peroneal tenography was performed to examine compression of the peroneal tendon sheath. The sheaths on the injured, painful side were compared to the contralateral sheath at the same level. The data was analyzed with bivariate correlation using SPSS 13.0 for Windows. Results: The injured calcanei were wider in all patients and the peroneus longus and brevis tendon sheaths were compressed on peroneal tenography in 68.9% (51 of 74). Statistics demonstrated significant correlations between compression of tendon sheaths and lateral hindfoot pain (Rs = 0.93, p < 0.001), and between increasing calcaneal width and lateral hindfoot pain(Rs = 0.665, p < 0.001). The severity of lateral hindfoot pain was directly correlated to tendon sheath impingement and indirectly related to calcaneal widening. Conclusion: Calcaneal widening following fracture union was the cause of compression of the peroneal tendons. Increasing compression correlated with increasing levels of lateral pain. Our study demonstrated the utility of peroneal tenography in identifying the presence of peroneal tendon impingement in patients with lateral pain following calcaneal fractures. Copyright © 2011 by the American Orthopaedic Foot & Ankle Society.