Hebei Northern College
Hebei Northern College
Li L.,Hebei Northern College |
Li L.,Chinese People's Liberation Army |
Li L.,Beijing Institute of Pharmacology and Toxicology |
Jiang X.-Y.,Chinese People's Liberation Army |
And 6 more authors.
Chinese Journal of Pharmacology and Toxicology | Year: 2017
OBJECTIVE: To investigate the effect of overexpession of 18 ku translocator protein (TSPO) on the hippocampal dentate gyrus. METHODS: Lentiviral (LV) vectors containing TSPO or the lentiviral sequence were infused into the hippocampus bilateral dentate gyri (2 × 108 TU • mL-1,1 μL per side) of mice. Behavioral tests were carried out. The anxiolytic-like behavior of mice was examined by such means as the elevated plus maze test, the staircase test, light dark box test for 12,14 and 16 d, two behavioral despair models, tail suspension test and the forced swimming test for 16 and 18 d, respectively. Western blotting and ELISA were used to evaluate the TSPO expression and the concentration of allopregnanolone in hippocampal tissue (3 mm in diameter around the injection site on both sides) at the end of tests. RESULTS: The results of behavioral experiments showed that TSPO overexpression group deneloped anxiolytic and antidepression-like behavior. LV-TSPO significantly increased the retention time in the central area [14 ± 4 vs (25 ± 12)s, P<0.05). LV-TSPO significantly increased the percentage of entry into open arms entries percentage and the percentage of time spent in open arms time without changing total entries and total time in the elevated plus-maze test [(13 ± 8)% vs (26 ± 18)%, P<0.05; (6±6)% vs (27±6)%, P<0.05)). LV-TSPO significantly decreased the number of rearings without changing the number of steps in staircase test (21 ±7 vs 12±5, P<0.05). LV-TSPO increased entries into the light area and retention time in light-dark transition test [(18±8)% vs (26±7)%, P<0.05;72±36 vs (191±90)s, P<0.05)) but significantly decreased immobility time in the tail suspension test and forced swimming test [94±33 vs (36±20)s, P<0.01; 137±36 vs (90±37)s, P<0.05)], without excitatory or inhibitory actions on the central nervous system. At the same time, the level of TSPO expression in hippocampal tissues (3 mm in diameter around the injection site on both sides) was significantly increased, so did the concentration of allopregnanolone (P<0.05). CONCLUSION: Overexpression of TSPO in the hippocamus dentate gyrus of mice can induce anxiolytic and antidepressant-like behavior, and the downstream allopregnanolone biosynthesis at least partially mediates the behavioral effects.
Feng P.,Hebei Northern College |
Zhang Z.-L.,First Affiliated Hospital |
Zhang Z.-H.,First Affiliated Hospital |
Zhang X.-L.,First Affiliated Hospital |
And 3 more authors.
Asian Pacific Journal of Tropical Medicine | Year: 2013
Objective: To evaluate the therapeutic effect of endostar (ED) combined with cisplatin(DDP) on model of C57BL/6 rats, and to further investigate the inhibiting mechanism of endostar from tumor angiogenesis. Methods: Lewis lung cancer cells were inoculated in C57BL/6 mouse, then the mouse were randomized into control group (group A), ED (group B), DDP (group C) and ED/DDP (group D). They were treated according to the plan. And the expressions of VEGF and Sema3A were evaluated by immunhistochemisty. Results: The weight of tumor increased in group A and B. It was decreased in group C and D. The tumor volume was increased in all the 4 groups. The VEGF expression of group D was obviously lower than the other group 3, but the Sema3A expressed of group D was significantly strengthener than the other group 3. The VEGF expression of group B and group D were obviously low especially in the 4th-8th days. Pearson correlated analysis showed that the expression VEGF and Sema3A were negatively correlated (. r=-0.72, P<0.05). Conclusions: ED combined with DDP could control the tumor growth effectively, and avoid weight loss. ED could reduce VEGF expression, and enhance Sema3A expression. Tumor vessel presents transient normalization. It is easy for DDP perfusion, and to kill tumor cells. © 2013 Hainan Medical College.
Sun M.,Hebei Northern College |
Yue C.,Hebei Northern College |
Ge J.,Hebei Northern College |
Mu X.,Hebei Zhangjiakou Great Wall Diary Corporation |
And 2 more authors.
Journal of Animal and Veterinary Advances | Year: 2012
The objective of the present study was to evaluate the effects of chitosan on serum GH, T3 and T4 in and to determine the appropriate supplemental dosage level of chitosan in dairy calves diet. A 30 days trial of dietary chitosan supplementation was reported. Twenty dairy calves were allotted at random to four groups at 10 days of age. Calves in the control group were fed the basal diet alone and calves in treatment groups I-III were fed the basal diet supplemented with 200, 400, 600 mg/day/h Chitosan (CTS), respectively for 30 days. The trial demonstrated that dietary chitosan supplementation significantly increased serum T3 level (p<0.05), when comparing chitosan groups I, II with the control group. Dietary supplementation of chitosan at 400 mg/day/h significantly increased serum T3 (p<0.05). Treatments with various levels of chitosan supplementation showed a tendency of increase in serum T4 and GH levels. So, the appropriate supplemental dosage of chitosan in the diets of dairy calves was 400 mg/day/h Chitosan (CTS). © Medwell Journals, 2011.