Yuan J.-G.,251 Hospital of Chinese PLA |
Zhou Z.-L.,Hebei Northern College |
Liu Y.-F.,Hebei Northern College |
Zhu Z.-A.,Shanghai University
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010
BACKGROUND: The bone marrow stem cell (MSC) transplant treatment have the obvious superiority to tradition graft treatment for bone nonunion, but how to obtain the concentrated and highly effective bone marrow mesenchymal stem cell, as well as the dose-effect relations to fracture healing need further discussions. OBJECTIVE: To observe the curative effect of bone nonunion by using autologous MSC transplant treatment, and to compare with autologous iliac bone graft. DESIGN, TIME AND SETTING: Randomized controlled analysis was performed from January 1999 to June 2005 in the Affiliated Second Hospital of Hebei Northern College. PARTICIPANTS: The admitting 140 patients with humerus and tibia fracture were divided into 2 groups at random, autologous iliac bone graft group and autologous MSC transplant group, with 70 patients in each group. METHODS: Under aseptic condition, autologous MSC transplant group received puncture through posterior superior iliac spine, extracting bone marrow 10-20 mL from different spots, separating MSC using the density gradient centrifugation method, and counting as 4×109 nucleated cells/mL under the microscope for later use. In the autologous iliac bone graft group, bone fracture end was implanted with the suitable amount of iliac bone, while autologous MSC transplant group with the mixture of decalcified bone matrix and MSC, followed by suture. After the transplantation, external fixation may assist for 4-6 weeks according to the fixed degree of internal fixation. MAIN OUTCOME MEASURES: 1 Bone callus formation and pain conditions in 2 groups at different time points after transplantation. 2 Comparison of bone healing time between 2 groups. ? Adverse events and side effects. RESULTS: According to intention-treatment analysis, experimental adopted 140 patients of humerus and tibia fractures, who all entered the final analysis. 1 Bone callus formation and pain at different time points post-surgery: At 1 month after transplantation, bone callus formation in the fracture end was not obvious in autogenous iliac bone graft group, and could be seen in autologous MSC transplant group, both groups of fractures exhibited tenderness. At 2 months after transplantation, bone callus formation was observed in autogenous iliac bone graft group, fracture tenderness was relieved compared with the previous condition; in autologous MSC transplant group, a large number of bone callus formed, fracture tenderness was not obvious. At 3 months after transplantation, there were a large number of bone callus formations in autogenous iliac bone graft group, with slight fracture tenderness; in the autologous MSC transplant group, continuous bone callus formation appeared, without fracture tenderness. 2 Bone healing time: The average healing time of autologous MSC transplant group was significantly shorter than autogenous iliac bone graft group [(5.5±1.5), (8.0±2.0) months, P < 0.05]. 3 Adverse events and side effects: Except 4 patients had iliac bone pain, all patients during the treatment had no infection and other complications, there were no re-fracture occurred at the follow-up of 8 months. CONCLUSION: The autologous MSC transplant treatment of exhibits a short duration and good effect for bone non-union, has obvious advantages over traditional bone graft.
Sun M.,Hebei Northern College |
Yue C.,Hebei Northern College |
Ge J.,Hebei Northern College |
Mu X.,Hebei Zhangjiakou Great Wall Diary Corporation |
And 2 more authors.
Journal of Animal and Veterinary Advances | Year: 2012
The objective of the present study was to evaluate the effects of chitosan on serum GH, T3 and T4 in and to determine the appropriate supplemental dosage level of chitosan in dairy calves diet. A 30 days trial of dietary chitosan supplementation was reported. Twenty dairy calves were allotted at random to four groups at 10 days of age. Calves in the control group were fed the basal diet alone and calves in treatment groups I-III were fed the basal diet supplemented with 200, 400, 600 mg/day/h Chitosan (CTS), respectively for 30 days. The trial demonstrated that dietary chitosan supplementation significantly increased serum T3 level (p<0.05), when comparing chitosan groups I, II with the control group. Dietary supplementation of chitosan at 400 mg/day/h significantly increased serum T3 (p<0.05). Treatments with various levels of chitosan supplementation showed a tendency of increase in serum T4 and GH levels. So, the appropriate supplemental dosage of chitosan in the diets of dairy calves was 400 mg/day/h Chitosan (CTS). © Medwell Journals, 2011.
Feng P.,Hebei Northern College |
Zhang Z.-L.,Hebei Northern College |
Zhang Z.-H.,Hebei Northern College |
Zhang X.-L.,Hebei Northern College |
And 3 more authors.
Asian Pacific Journal of Tropical Medicine | Year: 2013
Objective: To evaluate the therapeutic effect of endostar (ED) combined with cisplatin(DDP) on model of C57BL/6 rats, and to further investigate the inhibiting mechanism of endostar from tumor angiogenesis. Methods: Lewis lung cancer cells were inoculated in C57BL/6 mouse, then the mouse were randomized into control group (group A), ED (group B), DDP (group C) and ED/DDP (group D). They were treated according to the plan. And the expressions of VEGF and Sema3A were evaluated by immunhistochemisty. Results: The weight of tumor increased in group A and B. It was decreased in group C and D. The tumor volume was increased in all the 4 groups. The VEGF expression of group D was obviously lower than the other group 3, but the Sema3A expressed of group D was significantly strengthener than the other group 3. The VEGF expression of group B and group D were obviously low especially in the 4th-8th days. Pearson correlated analysis showed that the expression VEGF and Sema3A were negatively correlated (. r=-0.72, P<0.05). Conclusions: ED combined with DDP could control the tumor growth effectively, and avoid weight loss. ED could reduce VEGF expression, and enhance Sema3A expression. Tumor vessel presents transient normalization. It is easy for DDP perfusion, and to kill tumor cells. © 2013 Hainan Medical College.