Feng P.,Hebei Northern College |
Zhang Z.-L.,First Affiliated Hospital |
Zhang Z.-H.,First Affiliated Hospital |
Zhang X.-L.,First Affiliated Hospital |
And 3 more authors.
Asian Pacific Journal of Tropical Medicine | Year: 2013
Objective: To evaluate the therapeutic effect of endostar (ED) combined with cisplatin(DDP) on model of C57BL/6 rats, and to further investigate the inhibiting mechanism of endostar from tumor angiogenesis. Methods: Lewis lung cancer cells were inoculated in C57BL/6 mouse, then the mouse were randomized into control group (group A), ED (group B), DDP (group C) and ED/DDP (group D). They were treated according to the plan. And the expressions of VEGF and Sema3A were evaluated by immunhistochemisty. Results: The weight of tumor increased in group A and B. It was decreased in group C and D. The tumor volume was increased in all the 4 groups. The VEGF expression of group D was obviously lower than the other group 3, but the Sema3A expressed of group D was significantly strengthener than the other group 3. The VEGF expression of group B and group D were obviously low especially in the 4th-8th days. Pearson correlated analysis showed that the expression VEGF and Sema3A were negatively correlated (. r=-0.72, P<0.05). Conclusions: ED combined with DDP could control the tumor growth effectively, and avoid weight loss. ED could reduce VEGF expression, and enhance Sema3A expression. Tumor vessel presents transient normalization. It is easy for DDP perfusion, and to kill tumor cells. © 2013 Hainan Medical College.
Sun M.,Hebei Northern College |
Yue C.,Hebei Northern College |
Ge J.,Hebei Northern College |
Mu X.,Hebei Zhangjiakou Great Wall Diary Corporation |
And 2 more authors.
Journal of Animal and Veterinary Advances | Year: 2012
The objective of the present study was to evaluate the effects of chitosan on serum GH, T3 and T4 in and to determine the appropriate supplemental dosage level of chitosan in dairy calves diet. A 30 days trial of dietary chitosan supplementation was reported. Twenty dairy calves were allotted at random to four groups at 10 days of age. Calves in the control group were fed the basal diet alone and calves in treatment groups I-III were fed the basal diet supplemented with 200, 400, 600 mg/day/h Chitosan (CTS), respectively for 30 days. The trial demonstrated that dietary chitosan supplementation significantly increased serum T3 level (p<0.05), when comparing chitosan groups I, II with the control group. Dietary supplementation of chitosan at 400 mg/day/h significantly increased serum T3 (p<0.05). Treatments with various levels of chitosan supplementation showed a tendency of increase in serum T4 and GH levels. So, the appropriate supplemental dosage of chitosan in the diets of dairy calves was 400 mg/day/h Chitosan (CTS). © Medwell Journals, 2011.