Hebei North College

Zhangjiakou, China

Hebei North College

Zhangjiakou, China
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Liu Z.-H.,Hebei North College | Liu Y.-X.,First Affiliated Hospital of PLA General Hospital | Li J.-F.,First Affiliated Hospital of PLA General Hospital | Zhao Y.,Air Force General Hospital | And 3 more authors.
Chinese Journal of Pharmacology and Toxicology | Year: 2013

OBJECTIVE: To study the effect of suberoylanilide hydroxamic acid (SAHA) on survival and apoptosis of human paclitaxel-resistant ovarian cancer OC3/P cells. METHODS: OC3/P cells were treated by SAHA with the concentrations of 4, 8, 16, 32 and 64 μmol·L-1 for 6, 12, 24 or 48 h. Morphological changes of OC3/P cells were observed under inverted microscope, and the changes of cell nucleus were assessed by Giemsa-Wright staining. The viability of OC3/P cells was detected with MTT assay. The apoptosis rates of OC3/P cells were analyzed by AnnexinV-FITC/PI double staining assay with flow cytometry. Transmission electron microscopy was used for assessing the cells ultrastructure. RESULTS: After incubation with SAHA 4, 16 and 64 μmol·L-1 for 24 h, OC3/P cells became shrinkage, poor adherence, less refractive, which were more obvious with the increase in SAHA concentration. Giemsa-Wright staining showed that OC3/P cells became cytoplasmic agglutination and karyopyknosis after treated with SAHA 64 μmol-L -1 for 24 h. MTT assay revealed that SAHA could inhibit OC3/P cell survival in a concentration-dependent manner (P<0.05) and time-dependent manner (P<0.05). The inhibitory ratios of SAHA at concentration 64 μmol·L-1 for 6, 12 ,24 and 48 h were(7.1±1.9)%, (14.6±2.0)%, (36.8±2.7)% and (83.3±3.0)%, respectively(r=0.891, P<0.05), and SAHA 4-64 μmol·L-1 for 48 h were(28.8±1.2)%, (34.8±4.3)%, (52.3±2.8)%, (61.3±4.9)% and (83.3±3.0)%, respectively(r=0.966, P<0.05). The results of flow cytometry assay revealed that SAHA 4, 16 and 64 μmol·L-1 for 48 h could induce apoptosis of OC3/P cells (P<0.05, P<0.01). The apoptosis characteristics in OC3/P cells were also confirmed by transmission electron microscopy. CONCLUSION: SAHA can inhibit the survival and induce apoptosis of human paclitaxel-resistant ovarian cancer OC3/P cells.


Li T.-T.,Hebei North College | Xiu B.-S.,Chinese Institute of Basic Medical Sciences
Journal of International Pharmaceutical Research | Year: 2016

Objective To select the immunodominant epitope of human serum albumin (HSA) and provide the basis for setting up a special and rapid detecting method of HSA. Methods Bioinformatic method was used to compare protein sequences of human, pig, horse, ox, and ovine, and the immunodominant epitopes of HSA were predicted. The E.coli preferred codons were used todesign the DNA sequences of the selected epitopes. The genes of the epitopes were expressed after they were inserted into the PGEX-4T-2 vector. The recombinant antigens were identified and valued by HSA antibody with indirect ELISA. Results The length of the selected epitopes was H1 [126-162 amino acid (aa)], H2 (314-355aa), and H3 (373-424aa) and the relative molecular weight was 3.01×104, 3.06×104 and 3.17×104, respectively. The epitope of 373-424 aa were more active and its cross-reactivity IC50 of enzymelabeled antibody was 1.635 mg/L, which was higher than HSA (P<0.05). Conlusion The immunodominant epitope of HSA is obtained, which is significant for developing a rapid and special reagent of HSA. © 2016, Editorial office of Journal of International Pharmaceutical Research. All rights reserved.


Song X.,Zhejiang Agriculture And forestry University | Peng C.,University of Quebec at Montréal | Peng C.,Northwest Agriculture and Forestry University | Zhao Z.,University of Quebec at Montréal | And 5 more authors.
Atmospheric Environment | Year: 2014

We collected 139 estimates of the annual forest soil CO2 flux and 173 estimates of the Q10 value (the temperature sensitivity) assembled from 90 published studies across Chinese forest ecosystems. We analyzed the annual soil respiration (Rs) rates and the temperature sensitivities of seven forest ecosystems, including evergreen broadleaf forests (EBF), deciduous broadleaf forests (DBF), broadleaf and needleleaf mixed forests (BNMF), evergreen needleleaf forests (ENF), deciduous needleleaf forests (DNF), bamboo forests (BF) and shrubs (SF). The results showed that the mean annual Rs rate was 33.65t CO2ha-1year-1 across Chinese forest ecosystems. Rs rates were significantly different (P<0.001) among the seven forest types, and were significantly and positively influenced by mean annual temperature (MAT), mean annual precipitation (MAP), and actual evapotranspiration (AET); but negatively affected by latitude and elevation. The mean Q10 value of 1.28 was lower than the world average (1.4-2.0). The Q10 values derived from the soil temperature at a depth of 5cm varied among forest ecosystems by an average of 2.46 and significantly decreased with the MAT but increased with elevation and latitude. Moreover, our results suggested that an artificial neural network (ANN) model can effectively predict Rs across Chinese forest ecosystems. This study contributes to better understanding of Rs across Chinese forest ecosystems and their possible responses to global warming. © 2014 Elsevier Ltd.


Lu Y.,Hebei North College | Liu B.,Hebei North College
Chinese Journal of Cancer Prevention and Treatment | Year: 2012

OBJECTIVE: To investigate the expression of metastasis-associated gene 2(MTA2) in esophageal squamous cell carcinoma(ESCC), and relationship between expression and lymph node metastasis of ESCC. METHODS: Expressions of MTA2 was detected by immunohistochemical staining (SP) and flow cytometry (FCM) in tissues from 102 patients with ESCC and 12 normal esophageal mucosa. Analysis was performed in relationship with lymph node metastasis. RESULTS: The expression rate of MTA2 in ESCC was 68.6% (70/102). MTA2 was negative expressed in normal esophageal mucosa. The expression rate of MTA2 in esophageal esophageal with or without metastasis of lymph nodes were 68.2% (15/22) and 27.1% (13/48), and there was a significant deference (P=0.003). The expression of MTA2 was related to the lymphatic metastasis of ESCC, and its expression level was higher in patients with lymphatic metastasis. The protein relative level detected by FCM: the protein content of MTA2 were 252.11±30.22 in positive lymph node metastasis and 161.42±22.89 in negative group, There was a significant deference (t=15.11, P=0.000). CONCLUSION: The expression of MTA2 in ESCC is related with lymph node metastasis and MTA2 may act as a new symbol and target in the treatment of ESCC.


Li H.-J.,Hebei North College | Wang J.-M.,Hebei North College | Tian Y.-T.,Hebei North College | Bai M.-L.,Hebei North College | And 2 more authors.
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2013

OBJECTIVE: To study the effect of matrine on Fas, VEGF, and activities of telomerase of MCF-7 cells.METHODS: In vitro cultured human breast cancer MCF-7 cells were randomly divided into the experimental group and the control group. The matrine solution was added in cells of the experimental group. Equal volume of culture medium was added in cells of the control group or the negative control group. Zedoary Turmeric Oil, the telomerase inhibitor was added in cells of the positive control group. Morphological changes were observed under an inverted microscope. The telomerase activity was detected by TRAP-ELISA. Expressions of Fas and VEGF protein were detected by immunocytochemical assay.RESULTS: Matrine obviously inhibited the growth and induced apoptosis of breast cancer cells. MCF-7 cells were treated by matrine of different concentrations at 24, 48, and 72 h, the telomerase activity gradually decreased along with increased matrine concentration and prolonged action time, showing dose-effect and time-effect positive relations. Matrine could up-regulate Fas protein expression and downregulate VEGF protein expression of MCF-7 cells.CONCLUSION: Matrine showed obvious effect in inhibiting the growth of MCF-7 cells and promoting the apoptosis, which might be achieved by up-regulating the expression of Fas protein, inhibiting telomerase activity induced apoptosis of breast cancer cells, down-regulating the expression of VEGF protein, and inhibiting the tumor vascular formation.


Song X.,Zhejiang Agriculture And forestry University | Jiang H.,Zhejiang Agriculture And forestry University | Zhang Z.,Hebei North College | Zhou G.,Zhejiang Agriculture And forestry University | And 2 more authors.
Soil Biology and Biochemistry | Year: 2014

Being two important agents of global environmental change, elevated ultraviolet-B (UV-B) radiation derived from anthropogenic driven ozone depletion and enhanced nitrogen (N) deposition may strongly affect litter decomposition, a crucial factor in biogeochemical cycling. However, the interactive effects of both agents together on litter decomposition are still unclear even though each has been well-documented independently. We conducted a field-based experiment in subtropical China to investigate the combined effects UV-B radiation and N deposition on the decomposition of Moso bamboo (Phyllostachys pubescens) leaf litter over a 20 months period. It was found that the combined effect significantly accelerated litter decomposition, C loss, and lignin degradation as well as facilitating phosphorous (P) release, although it had no measurable effect on N release. Moreover, the interactive effects of both agents together far exceeded the effects of each separately. Results indicated that the positive combined effect of UV-B radiation and N deposition on litter decomposition and C loss could potentially impact Moso bamboo forest ecosystem C cycling. These findings provide a new perspective to further understand the interactive effects of global environmental changes on terrestrial ecosystem processes. © 2013 Elsevier Ltd.


Zhang H.-L.,Zhejiang Agriculture And forestry University | Song X.-Z.,Zhejiang Agriculture And forestry University | Zhang Z.-T.,Hebei North College | Jiang H.,Zhejiang Agriculture And forestry University | And 2 more authors.
Chinese Journal of Applied Ecology | Year: 2011

A litterbag experiment was conducted to study the decomposition of Cunninghamia lan-ceolata leaf litter under ambient and reduced UV-B radiation (22.1% below ambient). Comparing with ambient treatment, the reduced treatment decreased the decomposition rate of C. lanceolata leaf litter by 69.6% (P<0.001), making the relative contents of nitrogen (N), phosphorus (P), and lignin in the litter increased by 150%, 83.3%, and 13.8%, respectively, and the release of potassium (K) and carbon (C) slowed down. In the process of litter decomposition, photo-degradation of lignin didn't play crucial role. The results suggested that UV-B radiation could accelerate the decomposition rate of C. lanceolata leaf litter, promote the release of N, P, K, and C from it, and increase the nutrients turnover rate in litter layer as well as the carbon flux on the ground, giving potential effects on the function of C. lanceolata forest as a carbon source or sink in humid subtropical China.


Bai M.L.,Hebei North College
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2013

To observe the effects of deguelin on the apoptosis and proliferation of human esophageal cancer cell Ec-109, and to explore its possible mechanisms. Human esophageal cancer cells Ec-109 were in vitro cultured. They were divided into the blank control group, and 5, 10, 20, and 40 nmol/L deguelin groups. The inhibition on the proliferation was detected at 24, 48, and 72 h using CCK-8 assay. The early apoptosis rate at 24 h was detected by flow cytometry. The expressions of apoptosis-related proteins Bcl-2 and Bax were detected at 24 and 48 h respectively. Compared with the blank control group at the same point, the growth inhibition rate in all deguelin groups increased at 24, 48, and 72 h, showing statistical difference (P <0.05). The early apoptosis rate was 4.37% +/- 0.35%, 6.71% +/-0.14%, 15.62% +/- 0.21%, and 19.78% +/- 0.15% in 5, 10, 20, and 40 nmol/L deguelin groups, respectively, showing statistical difference when compared with that of the blank control group (1.10% +/- 0.08%, P < 0.05). Compared with the blank control group, Bcl-2 protein expression obviously decreased, and Bax protein expression obviously increased in 10, 20, and 40 nmol/L deguelin groups, showing statistical difference (P <0.05). The aforesaid indices were in time- and dose-dependent manners. Deguelin showed obvious effects on inhibiting the proliferation of Ec-109 cells and promoting their apoptosis, which was correlated with up-regulating Bax protein expression and down-regulating Bcl-2 protein expression.


Li X.-J.,Hebei North College | Zhai L.-P.,First Affiliated Hospital of Hebei North College | Liu J.-C.,First Affiliated Hospital of Hebei North College
Chinese Journal of Cancer Prevention and Treatment | Year: 2013

OBJECTIVE: To study the expression of Oct4 in esophageal squamous cell carcinoma (ESCC) and analyze the relationship between the expression of Oct4 and clinical prognosis. METHODS: Immunohistochemistry staining SP was used to detect the expression level of Oct4 in 58 cases of ESCC and 21 normal mucous memebrane. Subsequently the relationship between postoperative pathological features with the expression of Oct4 was analyzed. These 58 patients were followed for 8 years at least. Kaplan-Meier method and Log-Rank test were employed to explore the correlation between Oct4 expression and survival, and Cox regression analysis was performed to evaluate the connection between expression of Oct4 and prognosis. RESULTS: The positive expression rate of Oct4 in ESCC was 60.3%(35/58), obviously higher than that of normal esophageal (19.0%, 4/21, χ2=10.519, P=0.001). There was no significant difference in the expression of Oct4 among patients with age (χ2=0.023, P=0.879), gender (χ2=0.043, P=0.836), lymph mode metastasis (χ2=0.551, P=0.458), invasion of adventitia (χ2=0.364, P=0.546) and UICC stage (χ2=1.038, P=0.308). The expression of Oct4 in low differentiation group was higher than that in well or moderate differentiated(χ2=6.061, P=0.014). Oct4 expression was higher in deaths than that in survival cases (χ2=7.958, P=0.005). Patients with higher expression of Oct4 had significantly shorter survival time (χ2=7.703, P=0.006). The expression of Oct4 was obviously correlated with the prognosis of ESCC (RR=2.243, P=0.001). CONCLUSION: The expression of Oct4 is related to the differentiation and survival time of patients with ESCC, and it is an independent prognostic factor for ESCC.


PubMed | Hebei North College
Type: Journal Article | Journal: Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine | Year: 2013

To study the effect of matrine on Fas, VEGF, and activities of telomerase of MCF-7 cells.In vitro cultured human breast cancer MCF-7 cells were randomly divided into the experimental group and the control group. The matrine solution was added in cells of the experimental group. Equal volume of culture medium was added in cells of the control group or the negative control group. Zedoary Turmeric Oil, the telomerase inhibitor was added in cells of the positive control group. Morphological changes were observed under an inverted microscope. The telomerase activity was detected by TRAP-ELISA. Expressions of Fas and VEGF protein were detected by immunocytochemical assay.Matrine obviously inhibited the growth and induced apoptosis of breast cancer cells. MCF-7 cells were treated by matrine of different concentrations at 24, 48, and 72 h, the telomerase activity gradually decreased along with increased matrine concentration and prolonged action time, showing dose-effect and time-effect positive relations. Matrine could up-regulate Fas protein expression and downregulate VEGF protein expression of MCF-7 cells.Matrine showed obvious effect in inhibiting the growth of MCF-7 cells and promoting the apoptosis, which might be achieved by up-regulating the expression of Fas protein, inhibiting telomerase activity induced apoptosis of breast cancer cells, down-regulating the expression of VEGF protein, and inhibiting the tumor vascular formation.

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