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Li Y.,Hebei Medical University | Li Y.,Hebei Key Laboratory of Forensic Medicine | Wang Y.-L.,Hebei North University | Ni Z.-Y.,Hebei Medical University | And 12 more authors.
Zeitschrift fur Naturforschung - Section B Journal of Chemical Sciences | Year: 2011

Sinenxan H [2α,14β-diacetoxytaxa-4(20),11-diene-5α, 10β-diol,1] was isolated from the heartwood of Taxus cuspidata,for the first time from natural sources. Compound 1 was found to be readily converted into 2α-acetoxytaxa- 4(20),10,12(18),13-tetraen-5α-ol(2),thefirst example of a taxane with 10,11- and/or 13,14-double bond(s) and a conjugated triene system, in CDCl3.©2011 Verlag der Zeitschrift für Naturforschung, Tübing.


Ni Z.-Y.,Hebei Medical University | Wu Y.-B.,Hebei Medical University | Dong M.,Hebei Medical University | Zhang M.-L.,Hebei Medical University | And 10 more authors.
Zeitschrift fur Naturforschung - Section B Journal of Chemical Sciences | Year: 2011

Diabietane ether (1), a new abietane dimer connected by an ether linkage, and two known abietane diterpenoids (2 and 3) were isolated from the needles of Taxus cuspidata. © 2011 Verlag der Zeitschrift für Naturforschung, Tübingen.


Zhang P.,Hebei Medical University | Zhang P.,Hebei Key Laboratory of Forensic Medicine | Zhang F.,Air Force General Hospital | Wang Z.,Institute of Forensic Science of Supreme Peoples Procuratorate | And 3 more authors.
Chinese Journal of Chromatography (Se Pu) | Year: 2013

A rapid, specific and sensitive ultra performance liquid chromatography-mass spectrometry (UPLC-MS/MS) method was developed for the simultaneous determination of four trace alkaloids, including aeonitine (AC), hypaconitine (HA), mesaconitine (MA) and yunaconitine (YA) in human urine samples. UPLC-MS-MS system coupled with an electrospray ionization (ESI) source wasrperformed in multiple reaction monitoring (MRM) mode. The sample preparation was performed with hollow fiber microextraction (HF-LPME) prior to the analysis^ The enrichment factors of the four alkaloids were 102 - 301. The separation was applied on a Waters ACQUITY UPLC BEH C18 column (50 mm x2. 1 mm, 1. 7 μm) with a gradient elution of acetonitrile and 10 mmol/L NH4HCO3 as mobile phase. The retention times were less than 3 min. This method significantly improved the detection sensitivity, and the limits of quantitation were from 0. 01 to 0. 1 ng/L. The calibration curves were linear over the ranges of 0. 01 - 10 ng/L for AC, MA and YA, 0. 1 - 100 ng/L for HA in human urine samples, and the correlation coefficients were 0. 998 1, 0. 998 4, 0. 999 5 and 0. 998 6, respectively. The method was proved to be rapid and sensitive for aconitum alkaloid analysis in urine samples.


Feng T.,Hebei Key Laboratory of Forensic Medicine | Yang S.,Hebei Key Laboratory of Forensic Medicine | Yang S.,Hebei Medical University | Wen D.,Hebei Key Laboratory of Forensic Medicine | And 4 more authors.
NeuroReport | Year: 2014

Post-traumatic stress disorder (PTSD), a debilitating psychiatric disease characterized by invasive and persistent fear memories-induced stressful experience, is associated with numerous changes in neuroendocrine function. Here, we investigated whether PTSD-like symptoms are associated with changes in the cholecystokinin (CCK) system in the basolateral amygdala. We developed an animal model of PTSD using multiple foot shocks at 1.1 mA. The resulting conditioned fear response was severe (>80% freezing) and maintained for at least 28 days. The stress-associated neurotransmitters norepinephrine, dopamine, and corticotrophin-releasing hormone were elevated at 1 day after foot shock. CCK immunoreactivity and extracellular concentration as well as the expression of CCK receptors (CCK1R, CCK2R) increased progressively for 28 days following foot shock. Taken together, these results suggest that stress-induced activation of the CCK system in the BLA, which may contribute toward the development of PTSD-like symptoms.

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