Hebei Institute of Food Quality Supervision Inspection and Research

Shijiazhuang, China

Hebei Institute of Food Quality Supervision Inspection and Research

Shijiazhuang, China
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Ning Y.,State Key Laboratory of Food Science and Technology | Ning Y.,Jiangnan University | Li Q.,Hebei Institute of Food Quality Supervision Inspection and Research | Chen F.,Clemson University | And 4 more authors.
Bioresource Technology | Year: 2012

The effects of medium composition and culture conditions on the production of 6G-fructofuranosidase with value-added astaxanthin were investigated to reduce the capital cost of neo-fructooligosaccharides (neo-FOS) production by Xanthophyllomyces dendrorhous. The sucrose and corn steep liquor (CSL) were found to be the optimal carbon source and nitrogen source, respectively. CSL and initial pH were selected as the critical factors using Plackett-Burman design. Maximum 6G-fructofuranosidase 242.57U/mL with 5.23mg/L value-added astaxanthin was obtained at CSL 52.5mL/L and pH 7.89 by central composite design. Neo-FOS yield could reach 238.12g/L under the optimized medium conditions. Cost analysis suggested 66.3% of substrate cost was reduced compared with that before optimization. These results demonstrated that the optimized medium and culture conditions could significantly enhance the production of 6G-fructofuranosidase with value-added astaxanthin and remarkably decrease the substrate cost, which opened up possibilities to produce neo-FOS industrially. © 2011 Elsevier Ltd.


Hao X.-J.,Tsinghua University | Hao X.-J.,Inner Mongolia University of Technology | Zhou X.-H.,Tsinghua University | Zhou X.-H.,Collaborative Innovation Center for Advanced Water Pollution Control Technology and Equipment | And 6 more authors.
Sensors and Actuators, B: Chemical | Year: 2014

A sensitive, simple and rapid detection of trace amounts of melamine in milk products was achieved in a reusable, portable optical sensor system based on the principle of immunoassay and evanescent wave-excited fluorescence. The evanescent wave fiber-optic immunosensor (EWFI) employed a single-multi mode fiber optic coupler for light excitation and collection of fluorescence generated from the fiber optic probe. A reusable immunosurface of fiber probe was established to allow the performance of more than 300 assay cycles. Each assay cycle was less than 15 min. The typical calibration curves of the EWFI obtained for melamine (MA) had a detection limit of 5.14 μg/L with the detectable working range from 12.62 to 284.18 μg/L when the concentration of Cy5.5-labeled antibody was 0.2 μg/mL. The cross-reactivity against the organic compounds structurally similar to MA was negligible. The recoveries of MA in all sorts of dairy products ranged from 86.1% to 106.5% with relative standard deviation values of less than 4%, confirming the application potential in the measurement of MA in reality. The immunoassay performance of the EWFI was also validated with respect to conventional LC-MS/MS and the correlation between methods was in good agreement with the coefficient variation of less than 20% for majority samples. © 2014 Elsevier B.V.


Guo H.,Tsinghua University | Zhou X.,Tsinghua University | Zhou X.,Collaborative Innovation Center for Advanced Water Pollution Control Technology and Equipment | Zhang Y.,Hebei Institute of Food Quality Supervision Inspection and Research | And 5 more authors.
Sensors and Actuators, B: Chemical | Year: 2014

An indirect competitive immunoassay has been developed using the planar waveguide fluorescence immunosensor (PWFI) based on the principle of immunoreaction and total internal reflect fluorescent, offering simple, rapid and sensitive detection and quantification of melamine in buffer and milk products. The chip immobilized by BSA-Mel was reusable and highly resistive to non-specific binding of proteins. The regeneration of the sensor allows more than 100 assay cycles within 20 min for each assay cycle. For the standard curve, the limit of detection of 6.6 μg/L and the quantitative detection range of 26.6-517.5 μg/L were obtained when the concentration of antibody labeled by Cy5.5 was 2.4 μg/mL. The results meet the requirements for the maximum amount of melamine allowed in infant formula (1.0 mg/kg) and all the other foods (2.5 mg/kg) set by the WHO, which were better than most other standard methods. Few cross-reactivity effects were noted to occur among closely resembling pollutant molecules during the melamine determination process. Recoveries from the melamine-fortified blank samples were between 89.8 and 103.2% with relative standard deviation lower than 8.1%. The results were in good correlation (R2 = 0.9963) to those measured by LC-MS/MS. © 2013 Elsevier B.V.


Guo H.,Tsinghua University | Zhou X.,Tsinghua University | Zhang Y.,Hebei Institute of Food Quality Supervision Inspection and Research | Gu C.,Tsinghua University | And 2 more authors.
RSC Advances | Year: 2016

Methods based on optical biosensors for the investigation of biomolecular interactions between high-affinity antibodies and antigens has advanced over the last years. In this study, experimental and data analysis protocols were developed to determine the rate and equilibrium constants by using multiplexed planar waveguide fluorescence immunosensor. Four different antibodies were used as models in the test system. Antigen-BSAs were immobilized on the sensor surface, and the binding of specific antibodies labeled with Cy5.5 under certain conditions was measured. Sets of binding curves obtained with different antibody concentrations were analyzed by using the numerical integration of differential rate equations and global fitting applying by the one to one reaction model. As a result, the kinetic rate constants (ka and kd) and affinity (KD) can be determined for each antibody interaction under identical conditions. An indirect competitive immunoassay simulation model is also presented in the paper and shows the relationship between detection response, initial concentration of antibody and affinity constant. By analyzing the results and fitted in the indirect competitive immunoassay model, the optimized concentration of antibody and working ranges for detection can be estimated. Thus, the application of waveguide fluorescence immunosensors for protein interaction analysis is a promising and high throughput tool for obtaining data on the binding behavior between antibodies and antigens, and support the optimization in immunoassay. © The Royal Society of Chemistry 2016.


Guo H.,Tsinghua University | Zhou X.,Tsinghua University | Zhang Y.,Hebei Institute of Food Quality Supervision Inspection and Research | Song B.,Tsinghua University | And 2 more authors.
Food Chemistry | Year: 2016

Mycotoxins and industrial chemicals, such as aflatoxin M1 and melamine, now commonly exist in milk and cause potential health risks. This study presents an indirect competitive immunoassay through multiplex planar waveguide fluorescence immunosensor (MPWFI) for rapid, sensitive, and simultaneous detection and quantification of aflatoxin M1 and melamine by applying the principle of immunoreaction and total internal reflect fluorescent. Double-channel standard curves with appropriate logistic correlation (R2 > 0.99) were plotted, respectively. The working ranges (0.073-0.400 ng/mL and 26.38-270.00 ng/mL, respectively) were calculated, as well as the limit of detection (0.045 and 13.37 ng/mL, respectively), when two analytes were simultaneously detected. Both results satisfied the requirements for the maximum amount set by the WHO, which illustrated that the current method was better than some other standard methods. The recovery rates in the actual samples ranged from 85% to 103%, with relative standard deviations between 1.3% and 6.5%, which indicated high accuracy and repeatability. © 2015 Elsevier Ltd. All rights reserved.


PubMed | Tsinghua University, Renmin University of China, Inner Mongolia University of Technology and Hebei Institute of Food Quality Supervision Inspection and Research
Type: Journal Article | Journal: Sensors (Basel, Switzerland) | Year: 2015

Sulfadimidine (SM2) is a highly toxic and ubiquitous pollutant which requires rapid, sensitive and portable detection method for environmental and food monitoring. Herein, the use for the detection of SM2 of a portable optofluidics-based biosensing platform, which was used for the accurate detection of bisphenol A, atrazine and melamine, is reported for the first time. The proposed compact biosensing system combines the advantages of an evanescent wave immunosensor and microfluidic technology. Through the indirect competitive immunoassay, the detection limit of the proposed optofluidics-based biosensing platform for SM2 reaches 0.05 gL(-1) at the concentration of Cy5.5-labeled antibody of 0.1 gmL(-1). Linearity is obtained over a dynamic range from 0.17 gL(-1) to 10.73 gL(-1). The surface of the fiber probe can be regenerated more than 300 times by means of 0.5% sodium dodecyl sulfate solution (pH = 1.9) washes without losing sensitivity. This method, featuring high sensitivity, portability and acceptable reproducibility shows potential in the detection of SM2 in real milk and other dairy products.


Li H.,Hebei University | Li H.,Hebei Institute of Food Quality Supervision Inspection and Research | Sun H.,Hebei University | Zhang J.,Hebei Institute of Food Quality Supervision Inspection and Research | Pang K.,Hebei Institute of Food Quality Supervision Inspection and Research
Food Control | Year: 2013

A novel multiresidue analysis method is developed for highly sensitive and simultaneous determination of 16 sulphonamides (SAs), 4 acetyled metabolites, and trimethoprim in pork and mutton by rapid resolution liquid chromatography-tandem mass spectrometry (RRLC-MS/MS). The sample was extracted with acetonitrile under ultrasonication incubation, followed by solid phase extraction (SPE). The calibration curves showed good linearity with correlation coefficient (r) more than 0.998. The limit of quantification (LOQ) was 0.35-1.0 μg/kg, which can ensure to detect studied drugs at the maximum residue level (MRL) of 10 μg/kg. The mean recoveries at addition level of 1.0, 5.0 and 50 μg/kg were in the range of 68.3-104% with the relative standard deviation (RSD) of 3.5-9.2%. The intra-day precision (as RSD) for six determinations at 50 μg/kg spiked level within a day was in the range of 4.2-8.9%. The method is sensitive, accurate, convenient and rapid, and can be used for the qualitatively and quantitatively determination of multiresidue of the studied drugs in meat. © 2012 Elsevier Ltd.


Sun H.,Hebei University | Qi H.,Hebei University | Li H.,Hebei Institute of Food Quality Supervision Inspection and Research
Food Analytical Methods | Year: 2013

A rapid and effective method was developed in the first time for the determination of four sulfonamides (SAs) and their N4-acetylated metabolites in aquatic products using capillary electrophoresis with accelerated solvent extraction (ASE). The eight residues were extracted with acetonitrile at 70 °C under 10.3 MPa pressure and two static cycles with a static time of 5 min. The eight analytes can be baseline separated with 6 min. The standard curves for the determination of eight residues by the capillary electrophoretic method have good linearity (r 2 > 0.999). The method limits of quantification (LOQs) for N4-acetylsulfadiazine, sulfadiazine, sulfamerazine, sulfamethoxazole, sulfamethazine, N4-acetylsulfamethazine, N4-acetylsulfamerazine, and N4-acetylsulfamethoxazole in aquatic products were 26.4, 33.0, 33.0, 33.0, 39.6, 370, and 1,076 μg kg-1, respectively. Intra- and inter-day precision (RSD) were 0.4-2.2 % and 1.4-3.5 %, respectively. Their average recoveries with three spiked levels ranged from 83 % to 116 % with the RSDs less than 4 %. The proposed method provides a rapid and simple extraction procedure with high sensitivity and recoveries, and can permit routine detection of the studied SAs residues in aquatic products at the maximum residue level. © 2012 Springer Science+Business Media, LLC.


Sun H.-W.,Hebei University | Kang Z.-S.,Hebei University | Li H.,Hebei University | Li H.,Hebei Institute of Food Quality Supervision Inspection and Research
Fenxi Huaxue/ Chinese Journal of Analytical Chemistry | Year: 2010

A gel permeation chromatography-solid phase extraction-rapid resolution liquidchromatography-tandem mass spectrometry (GPC-SPE-RRLC-MS/MS) method was developed for the determination of 9 steroid hormone residues in beef tissue. The beef samples were enzymatically digested with β-glucuronidase/ arylsulfatase, and then extracted with tert-butyl methyl ether under ultrasonication incubation. Cleanup was carried out with GPC followed by a further HLB SPE. After C18 RRLC gradient elution separation withacetonitrile-0.1% aqueous formic acid as a mobile phase, the eluents were qualitatively and quantitatively determined under multireaction monitoring (MRM) scan type with tandemmass analyzer.The limit of quantification (LOQ) was 0.2-0.7 μg kg-1, andthe calibration curves showedgood linearity with correlation coefficients larger than 0.999. At the spiked levels of 0.2, 1.0 and 4.0 μg kg-1, the average recoveries were in the range of 81.4%-110%, and the relative standard deviations (RSDs) were in the range of 2.17%-9.82% (n = 7). The testresults of real sample showed this method is sensitive and accurate. The proposed methodwas used for the sensitive and accurate determination of 9 steroid hormone residues in high-fat and complex samples. Copyright © 2010.


Kou X.,Tianjin University | Wu M.,Tianjin University | Li L.,Tianjin University | Li L.,Tianjin Bohai Vocational Technology College | And 5 more authors.
Postharvest Biology and Technology | Year: 2015

Brown spot disorder is a disorder that limits the storage life of the 'Huangguan' pear (Pyrus pyrifolia, Nakai). This study aimed to evaluate the effects of postharvest dipping with 2% CaCl2 and coating with 1% pullulan on the development of brown spots during eight months of cold storage at 0°C. Analysis of calcium extracted using different solvents indicated that fruit dipped with CaCl2 had the highest concentrations of total calcium, water-soluble calcium, calcium pectin and calcium oxalate. Fruit coated with pullulan also had higher concentrations of water-soluble calcium, calcium pectin and calcium oxalate than control fruit, although the total calcium and calcium phosphate concentrations were similar to those of control fruit. Both treatments reduced the incidence of brown spots, inhibited the activities of PPO (polyphenol oxidase) and POD (peroxidase), increased the activities of CAT (catalase) and SOD (superoxide dismutase), and delayed the loss of phenolic compounds, compared with untreated controls. Concomitantly, concentrations of α-farnesene, CTs (conjugated trienes), and MDA (malondialdehyde) were maintained at lower levels in most tissues of treated fruit compared with those of control fruit. These results suggest that both CaCl2 and pullulan treatments inhibited the development of brown spots on 'Huangguan' pear by delaying the loss of the polyphenol substances and maintaining the structural integrity of cell membrane. © 2014 Elsevier B.V.

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