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Liang J.,Hebei Medical University | Dong Z.-M.,Hebei Medical University | Guo Y.-L.,Hebei Medical University | Han L.-J.,Hebei Cangzhou Central Hospital | And 2 more authors.
Tumor | Year: 2014

Objective: To detect the expression and methylation status of microRNA-203b (miR-203b) gene in esophageal squamous cell carcinoma (ESCC), and to investigate the role of miR-203b in the occurrence and development of ESCC. Methods: The expressions of miR-203b gene in esophageal cancer cell lines including TE1, TE13, Yes-2, Ec109 and T.TN treated or untreated with DNA methyltransferase inhibitor 5-aza-2′-detoxycytidine (5-aza-dC) and 54 ESCC tissue specimens and the corresponding non-cancerous tissue specimens were detected by real-time fuorescence quantitative-PCR (RFQ-PCR). The methylation status of miR-203b gene in five esophageal cancer cell lines treated or untreated with 5-aza-dC and 83 paired ESCC and the corresponding non-cancerous tissue specimens was detected by methylation-specific PCR (MSP). The relationship between the expression level and methylation status of miR-203b gene in ESCC tissues and the clinicopathologic features of ESCC patients and the correlation of expression and methylation status of miR-203b gene were analyzed by SPSS 13.0 software package. Results: The relatively low expression and the hypermethylation of miR-203b gene were detectable in the five esophageal cancer cell lines untreated with 5-aza-dC. After treatment with 5-aza-dC, the expression of miR-203b was significantly enhanced (P < 0.05), and the methylation level of miR-203b gene was decreased (P < 0.05) in esophageal cancer cell lines. The expression of miR-203b in ESCC tissues was significantly reduced as compared with that in the corresponding non-cancerous tissues (P < 0.05), and it was closely associated with the pathological differentiation of ESCC (P < 0.05). The methylation frequency of miR-203b gene promoter in ESCC tissues was significantly higher than that in the corresponding normal tissues (P < 0.05), and it was also associated with the pathological differentiation of ESCC (P < 0.05). The expression of miR-203b in ESCC tissues with miR-203b gene methylation was significantly lower than that in ESCC tissues with unmethylation of miR-203b gene (P < 0.05). Conclusion: Aberrant low-expression of miR-203b is closely related to the occurrence and development of ESCC. The methylation of miR-203b promoter may be one of the mechanisms for inactivation of miR-203b gene in ESCC. Copyright © 2014 by TUMOR. Source


Li Y.,Hebei Cangzhou Central Hospital | Xu Z.,Hebei Cangzhou Central Hospital | Liu Y.,Hebei Cangzhou Central Hospital | Peng W.,Hebei Cangzhou Central Hospital
Life Science Journal | Year: 2013

Objective: To investigate the changes in the vascular endothelial growth factor (VEGF) and C-reactive protein (CRP) levels before and after percutaneous coronary intervention treatment. Methods: 87 cases of coronary heart disease were treated with percutaneous transluminal coronary angioplasty (PTCA) and stent implantation in The Central Hospital of Cangzhou, Hebei Province from March 2011 to November 2012. CRP and VEGF changes were determined during the interventional Therapy. Results: Among 102 patients, the group of 87 patients with a total placement stent, two cases of double vessel disease in patients with descending artery stenosis, no-reflow phenomenon in 2 patients which abandoned PTCA guidewire, the surgical success rate was 95.4% (83 / 87). CRP and VEGF levels stent implantation done immediately after than before surgery (P <0.05), and 24 hours at peak below preoperative levels after one month, the difference was statistically significant (P <0.05). Conclusion: PTCA compared with preoperative CRP and VEGF levels increased, its variation may reflect the severity of coronary lesions and the degree of myocardial ischemia, monitoring of serum CRP and VEGF can understand the extent of the clinical therapeutic effects and inflammatory lesions. Source


Yang B.,Hebei Cangzhou Central Hospital | Zhao F.,Hebei Cangzhou Central Hospital | Zong Z.,Hebei Cangzhou Central Hospital | Yuan J.,Hebei Cangzhou Central Hospital | And 8 more authors.
Patient Preference and Adherence | Year: 2014

Background: This study was designed to investigate the preferences for treatment of lobectomy in Chinese lung cancer patients and differences in the psychological and social factors that influence treatment decision-making.Methods: One hundred and forty patients with stage I lung cancer were recruited from Hebei Cangzhou Central Hospital. Before surgery, the patients completed a questionnaire that surveyed their preferences for treatment and the relevant influencing factors. Differences in psychological and social characteristics were compared between lung cancer patients who chose video-assisted thoracoscopic surgery (VATS) and those who opted for open thoracotomy.Results: Among the 135 valid questionnaires, 79 patients preferred VATS and 56 patients chose open thoracotomy. Potential side effects, doctors’ recommendation, the prognosticated chance for cure, cosmesis, and financial burden influenced the patients’ decisions.Conclusion: The minimally invasive advantages of VATS, including lesser trauma to the chest wall, earlier remission of postoperative pain, faster recovery, less bleeding, and improved cardiopulmonary function made VATS more attractive to patients needing lobectomy for lung cancer. However, the choice of VATS over open thoracotomy is still influenced by the degree of prognosticated cure and the feasibility of surgery. © 2014 Yang et al. Source


Xu J.,Hebei Cangzhou Central Hospital | Wang G.,Hebei Cangzhou Central Hospital | Fu D.,Hebei Cangzhou Central Hospital | Su N.,Hebei Cangzhou Central Hospital | And 3 more authors.
Genetics and Molecular Research | Year: 2015

This study aims to investigate the value of high-resolution color Doppler ultrasonography (HR-CDU) in the evaluation of lower-extremity vasculopathy (LEV) and its related risk factors in type 2 diabetes mellitus (T2DM) patients. Two hundred forty T2DM patients were selected, who underwent lower-extremity arterial HR-CDU. The patients were divided into the LEV group (V group) and the nonvasculopathy group (A group). The V group was then divided into the mild (B group), moderate (C group), and severe (D group) subgroups. The relevant clinical parameters were simultaneously recorded. The results showed that the lesion-positive detection rate of HR-CDU was signifcantly higher than that of clinical examination. The age, disease duration, smoking history, blood pressure, blood sugar, fbrinogen (FIB), C-reactive protein (CRP), uric acid (UA), and urinary albumin excretion (UAE) in the V group were higher than in the N group. In the logistic regression analysis, smoking history, age, disease duration, FIB, UA, and fasting blood glucose were independent risk factors of T2DM LEV. The incidence of LEV in T2DM patients increased signifcantly with increasing age, UA, FIB, CRP, UAE, disease duration, and smoking history, and the vasculopathy level became more severe. In conclusion, age, disease duration, smoking history, blood pressure, blood sugar, FIB, CRP, UA, and UAE are the related risk factors of LEV in T2DM patients. © FUNPEC-RP. Source

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