Li Z.,Agricultural University of Hebei |
Zhang Y.,Agricultural University of Hebei |
Ge H.,Hebei Academy of science
Food Chemistry | Year: 2017
Surface browning is an important cause of deterioration of fresh-cut fruit during postharvest handling. In this paper, four pear cultivars with different extents of natural browning were selected to analyse the factors involved in browning. The main results are as follows: the lipoxygenase (LOX) activity of ‘Mantianhong’ and ‘Yali’ pears was higher accompanied by a stronger degree of browning, while the LOX activity in ‘Xueqing’ and ‘Xinli 7’ pears was lower, with less browning. A higher unsaturated fatty acid ratio of pear resulted in reduced browning. The cell membranes disappeared 30 min after being cut in ‘Mantianhong’ pear, which browns easily; however, the cell membranes were still intact 30 min after being cut in ‘Xueqing’ pear, which does not brown easily. Therefore, it can be assumed that the stability of the cell membrane plays an important role in inhibiting browning of fresh-cut pears. © 2017
Zou B.,Nanjing Agricultural University |
Zou B.,Cornell University |
Jia Z.,Hebei Academy of Science |
Tian S.,Nanjing Agricultural University |
And 4 more authors.
Functional Plant Biology | Year: 2013
Plant MYB transcription factors are implicated in resistance to biotic and abiotic stresses. Here, we demonstrate that an R2-R3 MYB transcription factor, AtMYB44, plays a role in the plant defence response to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (PstDC3000). The expression of AtMYB44 was upregulated upon pathogen infection and treatments with defence-related phytohormones. Transgenic plants overexpressing AtMYB44 (35S-Ms) exhibited greater levels of PR1 gene expression, cell death, callose deposition and hydrogen peroxide (H2O2) accumulation in leaves infected with PstDC3000. Consequently, 35S-M lines displayed enhanced resistance to PstDC3000. In contrast, the atmyb44 T-DNA insertion mutant was more susceptible to PstDC3000 and exhibited decreased PR1 gene expression upon infection. Using double mutants constructed via crosses of 35S-M lines with NahG transgenic plants and nonexpressor of pathogenesis-related genes1 mutant (npr1-1), we demonstrated that the enhanced PR1 gene expression and PstDC3000 resistance in 35S-M plants occur mainly through the salicylic acid signalling pathway. © 2013 CSIRO.
Xu J.,University of Florida |
Jia Z.,Hebei Academy of Science |
Knutson M.D.,University of Florida |
Leeuwenburgh C.,University of Florida
International Journal of Molecular Sciences | Year: 2012
Aging is associated with disturbances in iron metabolism and storage. During the last decade, remarkable progress has been made toward understanding their cellular and molecular mechanisms in aging and age-associated diseases using both cultured cells and animal models. The field has moved beyond descriptive studies to potential intervention studies focusing on iron chelation and removal. However, some findings remain controversial and inconsistent. This review summarizes important features of iron dyshomeostasis in aging research with a particular emphasis on current knowledge of the mechanisms underlying age-associated disorders in rodent models. © 2012 by the authors; licensee MDPI, Basel, Switzerland.
Wang X.-Z.,Hebei University |
He Y.-L.,Hebei University |
Dong L.-C.,Hebei University |
Zhao H.-Y.,Hebei Academy of science
Information Sciences | Year: 2011
Fuzzy measures and fuzzy integrals have been successfully used in many real applications. How to determine fuzzy measures is the most difficult problem in these applications. Though there have existed some methodologies for solving this problem, such as genetic algorithms, gradient descent algorithms and neural networks, it is hard to say which one is more appropriate and more feasible. Each method has its advantages and limitations. Therefore it is necessary to develop new methods or techniques to learn distinct fuzzy measures. In this paper, we make the first attempt to design a special particle swarm algorithm to determine a type of general fuzzy measures from data, and demonstrate that the algorithm is effective and efficient. Furthermore we extend this algorithm to identify and revise other types of fuzzy measures. To test our algorithms, we compare them with the basic particle swarm algorithms, gradient descent algorithms and genetic algorithms in literatures. In addition, for verifying whether our algorithms are robust in noisy-situations, a number of numerical experiments are conducted. Theoretical analysis and experimental results show that, for determining fuzzy measures, the particle swarm optimization is feasible and has a better performance than the existing genetic algorithms and gradient descent algorithms. © 2011 Elsevier Inc. All rights reserved.
Bai H.,CAS Institute of Geographical Sciences and Natural Resources Research |
Bai H.,University of Chinese Academy of Sciences |
Tao F.,CAS Institute of Geographical Sciences and Natural Resources Research |
Tao F.,Natural Resources Institute Finland Luke |
And 4 more authors.
Climatic Change | Year: 2016
Using the detailed field experiment data from 1981 to 2009 at four representative agro-meteorological experiment stations in China, along with the Agricultural Production System Simulator (APSIM) rice-wheat model, we evaluated the impact of sowing/transplanting date on phenology and yield of rice-wheat rotation system (RWRS). We also disentangled the contributions of climate change, modern cultivars, sowing/transplanting density and fertilization management, as well as changes in each climate variables, to yield change in RWRS, in the past three decades. We found that change in sowing/transplanting date did not significantly affect rice and wheat yield in RWRS, although alleviated the negative impact of climate change to some extent. From 1981 to 2009, climate change jointly caused rice and wheat yield change by −17.4 to 1.5 %, of which increase in temperature reduced yield by 0.0–5.8 % and decrease in solar radiation reduced it by 1.5–8.7 %. Cultivars renewal, modern sowing/transplanting density and fertilization management contributed to yield change by 14.4–27.2, −4.7– −0.1 and 2.3–22.2 %, respectively. Our findings highlight that modern cultivars and agronomic management compensated the negative impacts of climate change and played key roles in yield increase in the past three decades. © 2015, Springer Science+Business Media Dordrecht.
Liu R.,Nanjing Agricultural University |
Liu R.,Nanjing Normal University |
Chen L.,Nanjing Agricultural University |
Chen L.,Zhejiang Academy of Agricultural Sciences |
And 6 more authors.
Molecular Plant-Microbe Interactions | Year: 2011
AtMYB44 is a transcription factor that functions in association with the ethylene-signaling pathway in Arabidopsis thaliana. The pathway depends on ETHYLENE INSENSITIVE2 (EIN2), an essential component of ethylene signaling, to regulate defense responses in the plant following treatment with HrpNEa, a harpin protein from a bacterial plant pathogen. Here, we show that AtMYB44 regulates induced expression of the EIN2 gene in HrpNEa-treated Arabidopsis plants. A HrpNEa and ethylene-responsive fragment of the AtMYB44 promoter is sufficient to support coordinate expression of AtMYB44 and EIN2 in specific transgenic Arabidopsis. In the plant, the AtMYB44 protein localizes to nuclei and binds the EIN2 promoter; the HrpNEa treatment promotes AtMYB44 production, binding activity, and transcription of AtMYB44 and EIN2. AtMYB44 overexpression results in increased production of the AtMYB44 protein and the occurrence of AtMYB44-EIN2 interaction under all genetic backgrounds of wild-type Arabidopsis and the etr1-1, ein2-1, ein3-1, and ein5-1 mutants, which have defects in the ethylene receptor ETR1 and the signal regulators EIN2, EIN3, and EIN5. However, AtMYB44 overexpression leads to enhanced EIN2 expression only under backgrounds of wild type, ein3-1, and ein5-1 but not etr1-1 and ein2-1, suggesting that ethylene perception is necessary to the regulation of EIN2 transcription by AtMYB44. © 2011 The American Phytopathological Society.
Xi C.,Hong Kong Polytechnic University |
Hongxing Y.,Hong Kong Polytechnic University |
Lin L.,Hong Kong Polytechnic University |
Jinggang W.,Hebei University of Engineering |
Wei L.,Hebei Academy of science
Energy | Year: 2011
This paper presents experimental studies on a solar-assisted ground coupled heat pump (SAGCHP) system for space heating. The system was installed at the Hebei Academy of Sciences in Shijiazhuang (lat. N38°03', long. E114°26'), China. Solar collectors are in series connection with the borehole array through plate heat exchangers. Four operation modes of the system were investigated throughout the coldest period in winter (Dec 5th to Dec 27th). The heat pump performance, borehole temperature distributions and solar colleting characteristics of the SAGCHP system are analyzed and compared when the system worked in continuous or intermittent modes with or without solar-assisted heating. The SAGCHP system is proved to perform space heating with high energy efficiency and satisfactory solar fraction, which is a promising substitute for the conventional heating systems. It is also recommended to use the collected solar thermal energy as an alternative source for the heat pump instead of recharging boreholes for heat storage because of the enormous heat capacity of the earth. © 2011 Elsevier Ltd.
Zhang X.,Agricultural University of Hebei |
Zhang X.,Hebei Academy of Science |
Guo W.,Agricultural University of Hebei
International Journal of Biological Sciences | Year: 2011
There are many more glycoproteins in Helicoverpa armigera peritrophic membrane than midgut separated by SDS-PAGE analysis after Periodic acid-Schiff (PAS) and coomassie staining. The peritrophic membrane (PM) of H. armigera larvae contains about forty associated proteins. A cDNA library was constructed from H. armigera midgut mRNA to study the new target for pest biocontrol. An antiserum against Spodoptera exigua integral/total PM proteins cross reacted with several H. armigera PM proteins and was used to isolate a complete cDNA encoding an insect intestinal mucin (HaIIM86). The deduced protein sequence of the cDNA contained one potentially glycosylated, mucin-like domain, five cysteine-rich chitin-binding domains (CBDs) and two D-G rich regions. Mucin domain was lined between the first and second CBDs; the two additional D-G rich regions were proposed to internal reside at the amino terminus of the protein flanked by three cysteine-rich CBDs. HaIIM86 contains two D-G-rich tandem repeat domains flanked by cysteine-rich sequences in peritrophic membrane proteins which is not present in all the currently known PM proteins. Howerer the functions of D-G rich domains require further investigation. HaIIM86 was shown as 200kDa protein by SDS-PAGE analysis and appeared to be associated with the PM. HaIIM86 has chitin-binding activity and can be degraded into 90 and 70 kDa by HaGV Enhancin in vivo. The finding has shown that HaIIM86 is the target substrate for enhancin and the potential target for pest control. © Ivyspring International Publisher.
Xu C.,Hebei Academy of science
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2011
Quorum-sensing (QS) is a regulatory mechanism with which bacteria regulate the gene expression according to their population density. Pseudomonas aeruginosa regulates the expression of multiple genes via a hierarchical quorum-sensing cascade through LasR and RhlR and their cognate signal molecules N-(3-oxododecanoyl)-L-homoserine lactone (30-C12-HSL) and N-(butanoyl)-L-homoserine lactone (C4-HSL). It aims to explore the regulation of QS on biosynthesis of polyhydroxyalkanoates (PHA) in P. aeruginosa. Wild-type P. aeruginosa PA01 and its QS mutants were used to investigate the effects of quorum-sensing on biosynthesis of PHA by GC and real-time PCR at physiological and molecular level. After treated with QS signal molecule synthesis inhibitor azithromycin, the accumulation of PHA significantly decreased in P. aeruginosa PA01 and its QS mutant strains. The content of PHA in C4-HSL synthase gene rhlI mutant strain PA0210 had no significant difference compared with that of the wild type. However, the PHA contents were significantly affected in 30C12-HSL synthase gene lasl mutant strain PA055, 30C12-HSL transcriptional regulator gene lasR mutant strain PA056 and lasI/lasR double mutant strain PA057. PHA synthase gene phaC1 expression exhibited a significant reduction in lasI mutant and lasR mutant strains. 30C12-HSL signal molecules complementary experiment shows that the expression of phaC1 can be recovered to the level of the wild type, but the synthesis of PHA is only partially restored in lasI mutant strain. The results implicates that lasI/lasR system might be involved in the regulation of intracellular PHA biosynthesis in P. aeruginosa PA01.
Zhao Q.,Hebei Academy of science |
Song S.-S.,Hebei Academy of science
Plant Physiology Communications | Year: 2010
There is a complex interaction between plant and bacteria. Bacterial quorum sensing signals, N-acylhomoserine lactones (AHLs), are also important signals mediating plant and bacterial interaction. AHLs play an important role on plant growth and development. In this review, we would discuss recent findings in the regulation of plant growth and development by AHLs. It would be benefit for us to understand plant communication mechanism with bacteria completely, and to guide on promoting plant growth and enhancing crops production in practice.