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Zheng S.-Z.,Hebei Normal University | Liu Y.-L.,Hebei Normal University | Li B.,Hebei Normal University | Shang Z.-L.,Hebei Normal University | And 2 more authors.
Plant Journal | Year: 2012

Intracellular calcium (Ca2+) increases rapidly after heat shock (HS) in the Ca2+/calmodulin (Ca2+/CaM) HS signal transduction pathway: a hypothesis proposed based on our previous findings. However, evidence for the increase in Ca2+ after HS was obtained only through physiological and pharmacological experiments; thus, direct molecular genetic evidence is needed. The role of phosphoinositide-specific phospholipase C (PI-PLC) is poorly understood in the plant response to HS. In this work, atplc9 mutant plants displayed a serious thermosensitive phenotype compared with wild-type (WT) plants after HS. Complementation of atplc9 with AtPLC9 rescued both the basal and acquired thermotolerance phenotype of the WT plants. In addition, thermotolerance was even improved in overexpressed lines. The GUS staining of AtPLC9 promoter:GUS transgenic seedlings showed that AtPLC9 expression was ubiquitous. The fluorescence distribution of the fusion protein AtPLC9 promoter:AtPLC9:GFP revealed that the subcellular localization of AtPLC9 was restricted to the plasma membrane. The results of a PLC activity assay showed a reduction in the accumulation of inositol-1,4,5-trisphosphate (IP 3) in atplc9 during HS and improved IP3 generation in the overexpressed lines. Furthermore, the heat-induced increase in intracellular Ca2+ was decreased in atplc9. Accumulation of the small HS proteins HSP18.2 and HSP25.3 was downregulated in atplc9 and upregulated in the overexpressed lines after HS. Together, these results provide molecular genetic evidence showing that AtPLC9 plays a role in thermotolerance in Arabidopsis. © 2011 Blackwell Publishing Ltd.

Li B.H.,Hebei Academy of Agriculture and Forestry Science
Weed Science | Year: 2010

Research was conducted to establish a method to investigate the resistance level of flixweed to tribenuron-methyl and the evolved biochemical resistance mechanism. Four resistant biotypes were collected from wheat fields in Mazhuangcun, Jiacun, Dishangcun, and Bafangcun in the Hebei province of China where tribenuron-methyl had been continuously used for more than 10 yr. Two susceptible biotypes were collected from wheat fields where tribenuron-methyl was never applied. Different biotypes were assessed by petri-dish bioassay, whole-plant bioassay, and acetolactate synthase (ALS) assay. Comparisons of data indicated a similarity between methods and that experiments demonstrated that petri-dish bioassay was a feasible method to identify flixweed resistant to tribenuron-methyl. Data indicated differences among the flixweed biotypes when assessed by the petri-dish bioassay, whole-plant bioassay, or ALS enzyme assay, and a close association was obtained for the three bioassay methods. ALS resistance varied by biotypes with Mazhuangcun>Jiacun>Dishangcun> Bafangcun. Target-site enzyme assay data indicated that the resistant biotype's enhanced ALS activity was the biochemical mechanism that induced flixweed's evolved resistance to tribenuron-methyl. The concentrations of tribenuron-methyl causing 50 inhibition of ALS activity of the four resistant biotypes were 1,359, 513, 184, and 164 nM; in the susceptible biotypes these concentrations were 64 and 65 nM. Resistance indexes were 21, 8, 3, and 3 for Mazhuangcun, Jiacun, Dishangcun, and Bafangcun biotypes, respectively. © 2010 Weed Science Society of America.

Hebei Academy Of Agriculture And Forestry Science | Date: 2012-09-14

The present invention is a method of shoot apical meristem transformation for monocot plant via sufficient and micro wounding (SMW). The technical process includes: expose the apical meristem by removing the coleoptile away when the shoot grows to 0.2-2 cm after 1-2 days of seed germination; make sufficient and micro wounding transformation to the apical meristem by stabbing and brushing for 2-3 times using the SMW brush having 100-5000 bristles which is 4-20 m in diameter for each one and 0.5-3 mm in exposed length, and dipped with the

Hebei Academy Of Agriculture And Forestry Science | Date: 2014-11-20

The disclosure relates to a glutinous millet nutrition milk taking glutinous millet as raw material, and preparation process thereof, and particularly relates to a glutinous millet nutrition milk or compound glutinous millet nutrition milk taking the rust-proofing and glutinous foxtail millet variety of Ji Chuang 1 glutinous millet as raw material and preparation process thereof. The glutinous millet nutrition milk or compound glutinous millet nutrition milk prepared by the method has the characteristics that the emulsion is stable, it will neither become aged nor coagulate or precipitate after long-term storage, and it does not need to add any anticoagulant stabilizer, which is more environmental friendly and healthy; its products utilize the rich nutrition of the millet, digests and absorbs easily, and tastes delicately.

Gao F.,Hebei Normal University | Gao F.,Hebei Academy of Agriculture and Forestry Science | Gao F.,CAS Institute of Genetics and Developmental Biology | Han X.,Hebei Normal University | And 6 more authors.
Plant Journal | Year: 2012

An increased concentration of cytosolic calcium ions (Ca2+) is an early response by plant cells to heat shock. However, the molecular mechanism underlying the heat-induced initial Ca2+ response in plants is unclear. In this study, we identified and characterized a heat-activated Ca 2+-permeable channel in the plasma membrane of Arabidopsis thaliana root protoplasts using reverse genetic analysis and the whole-cell patch-clamp technique. The results indicated that A. thaliana cyclic nucleotide-gated ion channel 6 (CNGC6) mediates heat-induced Ca2+ influx and facilitates expression of heat shock protein (HSP) genes and the acquisition of thermotolerance. GUS and GFP reporter assays showed that CNGC6 expression is ubiquitous in A. thaliana, and the protein is localized to the plasma membrane of cells. Furthermore, it was found that the level of cytosolic cAMP was increased by a mild heat shock, that CNGC6 was activated by cytosolic cAMP, and that exogenous cAMP promoted the expression of HSP genes. The results reveal the role of cAMP in transduction of heat shock signals in plants. The correlation of an increased level of cytosolic cAMP in a heat-shocked plant with activation of the Ca2+ channels and downstream expression of HSP genes sheds some light on how plants transduce a heat stimulus into a signal cascade that leads to a heat shock response. © 2012 Blackwell Publishing Ltd.

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