Health Research Institute Of Santiago Of Compostela Idis Isciii

Santiago de Compostela, Spain

Health Research Institute Of Santiago Of Compostela Idis Isciii

Santiago de Compostela, Spain
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Fernandez-Ferreiro A.,University of Santiago de Compostela | Fernandez-Ferreiro A.,Health Research Institute Of Santiago Of Compostela Idis Isciii | Santiago-Varela M.,Hospital Of Conxo | Gil-Martinez M.,Hospital Of Conxo | And 12 more authors.
Journal of Ocular Pharmacology and Therapeutics | Year: 2017

Purpose: Acanthamoeba keratitis causes frequent epithelial lesions that fully expose the corneal stroma. The aim of this study was to determine the toxic profile of chlorhexidine and propamidine eye drops. Methods: We used primary human keratocytes in cell culture in combination with a novel technology that evaluates dynamic real-time cytotoxicity through impedance analysis. Additional studies such as a classic cell viability test (WST-1®), a bovine corneal opacity and permeability assay, and an irritation eye study (Hen's Egg Test [HET]) have been made. Results: Both eye drop formulations showed a time-and concentration-dependent toxicity profile, in which long periods and high concentrations were more detrimental to cells. In prolonged times of exposure, propamidine is more harmful to cells than chlorhexidine. On the contrary, no irritation has been detected in using the HET-chorioallantoic membrane test and no alterations in the corneal transparency nor permeability was produced by the treatment with both eye drops. Conclusions: In culture assay, chlorhexidine eye drops have proven to be less cytotoxic than Brolene® for a long contact period of time, but no signs of irritation or alterations in transparency or permeability have been observed in the cornea after both treatments. © 2017, Mary Ann Liebert, Inc.


Fernandez-Ferreiro A.,University of Santiago de Compostela | Fernandez-Ferreiro A.,Health Research Institute Of Santiago Of Compostela Idis Isciii | Santiago-Varela M.,Rua Ramon Baltar S n | Gil-Martinez M.,Rua Ramon Baltar S n | And 10 more authors.
International Journal of Pharmaceutics | Year: 2015

Non-steroidal anti-inflammatory drug (NSAID) eye drops are widely used to treat ocular inflammatory conditions related to ophthalmic surgical procedures, such as pseudophakic cystoid macular edema, and they have been used for off-label treatments. The most commonly used NSAIDs are diclofenac and ketorolac and the new molecules bromfenac and nepafenac have also been used. We used primary human keratocytes in cell culture in combination with a novel technology that evaluates dynamic real-time cytotoxicity through impedance analysis. This study also included classic cell viability tests (WST-1® and AlamarBlue®), wound healing assay, Hen's Egg Test and an ex vivo histopathological assay. NSAIDs were shown to have important cytotoxicities and to retard the healing response. Furthermore, the new eye drops containing bromfenac and nepafenac were more cytotoxic than the more classical eye drops. Nevertheless, no immuno-histochemical changes or acute irritation processes were observed after the administration of any eye drops tested. Due to cytotoxicity and the total absence of discomfort and observable injuries after the administration of these drugs, significant corneal alterations, such as corneal melts, can develop without any previous warning signs of toxicity. © 2015 Elsevier B.V. All rights reserved.


PubMed | Obesidomic Group Institute Investigacion Sanitaria IDIS ISCIII, University of Santiago de Compostela, Health Research Institute Of Santiago Of Compostela Idis Isciii and Xerencia de Xestion Integrada de Santiago de Compostela
Type: Comparative Study | Journal: International journal of pharmaceutics | Year: 2015

Non-steroidal anti-inflammatory drug (NSAID) eye drops are widely used to treat ocular inflammatory conditions related to ophthalmic surgical procedures, such as pseudophakic cystoid macular edema, and they have been used for off-label treatments. The most commonly used NSAIDs are diclofenac and ketorolac and the new molecules bromfenac and nepafenac have also been used. We used primary human keratocytes in cell culture in combination with a novel technology that evaluates dynamic real-time cytotoxicity through impedance analysis. This study also included classic cell viability tests (WST-1() and AlamarBlue()), wound healing assay, Hens Egg Test and an ex vivo histopathological assay. NSAIDs were shown to have important cytotoxicities and to retard the healing response. Furthermore, the new eye drops containing bromfenac and nepafenac were more cytotoxic than the more classical eye drops. Nevertheless, no immuno-histochemical changes or acute irritation processes were observed after the administration of any eye drops tested. Due to cytotoxicity and the total absence of discomfort and observable injuries after the administration of these drugs, significant corneal alterations, such as corneal melts, can develop without any previous warning signs of toxicity.

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