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Ashton L.,University of Manchester | Hogwood C.E.M.,University of Kent | Tait A.S.,University College London | Kuligowski J.,University of Valencia | And 5 more authors.
Journal of Chemical Technology and Biotechnology | Year: 2015

BACKGROUND: Recent advances in Raman spectroscopy have resulted in the development of rapid, in situ Raman probes that can identify and allow the assessment of the quality of complex constituents in mammalian cell culture. One specific Raman technique, UV resonance Raman (UVRR) spectroscopy, has potential as a probe for residual cellular DNA and RNA in mammalian cell culture medium. RESULTS: Variations in DNA and RNA UVRR spectral profiles of medium-cellular footprint samples were identified and related to time of harvest and increased cell lysis that is associated with a loss in cell viability. Increased DNA and RNA were also observed in the cell culture supernatant in response to sodium butyrate treatment. Variation in DNA and RNA profiles as a result of both primary and secondary clarification methods during downstream bioprocessing could also be determined with UVRR spectroscopy. CONCLUSIONS: This study has demonstrated the utility of UVRR spectroscopy as a tool to monitor variations in residual DNA and RNA that may contaminate cell culture medium. Application of this technique has the potential for both improvement of recovery techniques and assurance of reliable clearance of DNA/RNA to acceptable safety levels. © 2014 Society of Chemical Industry.

Beskers T.F.,Karlsruhe Institute of Technology | Beskers T.F.,PSS Polymer Standards Service GmbH | Brandstetter M.,Vienna University of Technology | Kuligowski J.,Health Research Institute Hospital la Fe | And 3 more authors.
Analyst | Year: 2014

This work introduces a tunable mid-infrared (mid-IR) external cavity quantum cascade laser (EC-QCL) as a new molecular specific detector in liquid chromatography. An EC-QCL with a maximum tunability of 200 cm-1 (1030-1230 cm-1) was coupled to isocratic high performance liquid chromatography (HPLC) for the separation of sugars with a cation exchange column (counter ion: Ca2+) and distilled water as the mobile phase. Transmission measurements in a 165 μm thick flow cell allowed for on-line coupling and independent quantification of glucose, fructose and sucrose in the concentration range from 5 mg mL-1 to 100 mg mL-1 in several beverages. The results obtained with the EC-QCL detector were found to be in good agreement with those obtained using a differential refractive index detector as a reference. The standard deviation of the method for the linear calibration was better than 5 mg mL-1 for all sugars and reached a minimum of 1.9 mg mL-1, while the DRI detector reached a minimum of 1 mg mL-1. Besides the quantification of sugars for which a calibration was performed, also chromatographic peaks of other components could be identified on the basis of their IR absorption spectra. This includes taurine, ethanol, and sorbitol. This journal is © the Partner Organisations 2014.

Perez-Guaita D.,University of Valencia | Kuligowski J.,Health Research Institute Hospital la Fe | Quintas G.,Leitat Technological Center | Garrigues S.,University of Valencia | De La Guardia M.,University of Valencia
Applied Spectroscopy | Year: 2013

A new method is proposed for the elimination of the spectral contribution of two atmospheric gases (CO2 and H2O) in Fourier transform infrared (FT-IR) spectra of clinical samples. The algorithm is based on the measurement of reference spectra of H2O followed by an automatic calculation of the spectral contribution of the above-mentioned gases to the sample spectra. Then this contribution is compensated by spectral subtraction. Attenuated total reflectance FT-IR spectra of serum and urine samples in the presence of atmospheric gases were corrected and compared with spectra obtained with an N2 purge. Visual inspection of the spectra as well as calculated noise levels confirmed that the method compensated for the contribution of CO2 and H2O to serum and urine spectra to a great extent. As compared with the correction performed by a commercial software package, the proposed method clearly improved the correction of CO 2 and provided similar results for H2O compensation. Correlation values between corrected spectra and those obtained under N 2-purged conditions in the fingerprint region were higher than 0.995, and noise values in regions where samples did not absorb confirmed the suitability of the correction. The proposed method is fast, easy, and extensible to other gaseous interferences, devices, and acquisition conditions. © 2013 Society for Applied Spectroscopy.

Vento M.,Polytechnic University of Valencia | Vento M.,Health Research Institute Hospital la Fe | Escobar J.,Health Research Institute Hospital la Fe | Cernada M.,Health Research Institute Hospital la Fe | And 2 more authors.
Clinics in Perinatology | Year: 2012

This article describes aerobic metabolism, oxygen free radicals, antioxidant defenses, oxidative stress, inflammatory response and redox signaling, the fetal to neonatal transition, arterial oxygen saturation, oxygen administration in the delivery room, oxygen during neonatal care in the NICU, evolving oxygen needs in the first few weeks of life, and complications that can occur when infants go home from the hospital on oxygen. © 2012 Elsevier Inc.

Jimenez-Serrano S.,Polytechnic University of Valencia | Tortajada S.,Polytechnic University of Valencia | Tortajada S.,Health Research Institute Hospital la Fe | Garcia-Gomez J.M.,Polytechnic University of Valencia | Garcia-Gomez J.M.,Health Research Institute Hospital la Fe
Telemedicine and e-Health | Year: 2015

Background: Postpartum depression (PPD) is a disorder that often goes undiagnosed. The development of a screening program requires considerable and careful effort, where evidence-based decisions have to be taken in order to obtain an effective test with a high level of sensitivity and an acceptable specificity that is quick to perform, easy to interpret, culturally sensitive, and cost-effective. The purpose of this article is twofold: first, to develop classification models for detecting the risk of PPD during the first week after childbirth, thus enabling early intervention; and second, to develop a mobile health (m-health) application (app) for the Android® (Google, Mountain View, CA) platform based on the model with best performance for both mothers who have just given birth and clinicians who want to monitor their patient's test. Materials and Methods: A set of predictive models for estimating the risk of PPD was trained using machine learning techniques and data about postpartum women collected from seven Spanish hospitals. An internal evaluation was carried out using a hold-out strategy. An easy flowchart and architecture for designing the graphical user interface of the m-health app was followed. Results: Naive Bayes showed the best balance between sensitivity and specificity as a predictive model for PPD during the first week after delivery. It was integrated into the clinical decision support system for Android mobile apps. Conclusions: This approach can enable the early prediction and detection of PPD because it fulfills the conditions of an effective screening test with a high level of sensitivity and specificity that is quick to perform, easy to interpret, culturally sensitive, and cost-effective. © 2015, Mary Ann Liebert, Inc.

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