Health Innovation Institute

Chiang Mai, Thailand

Health Innovation Institute

Chiang Mai, Thailand
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Pengkumsri N.,Chiang Mai University | Sivamaruthi B.S.,Chiang Mai University | Sirilun S.,Chiang Mai University | Peerajan S.,Health Innovation Institute | And 3 more authors.
Food Science and Technology | Year: 2017

Beta-glucan (BG) is a conserved cell wall components of bacteria, fungi, and yeast. BG is an immunomodulator and stimulates the host immune system. This study was performed to screen Saccharomyces cerevisiae strain with high BG, extraction of BG using different chemical extraction methods, composition analysis of BG, and evaluation of the immunomodulatory effect of high-quality BG using mice model. Ten yeast strains were screened for high BG content using total glucan extraction kit and were subjected to FT-IR analysis. The kit based extraction revealed that HII31 showed a high content of total glucan and BG. HII31 cells were subjected to four different acid/base extractions, which indicated that combination of a strong base (NaOH) and weak acid (CH3COOOOH) extraction recovered high BG and a high ratio of polysaccharide, protein, and lipid. Further, the immunomodulatory effect of the selected BG was evaluated using mice, which suggested that low dose of HII31-BG induces the expression of selected pro-inflammatory (IL-17, IFN-γ) and anti-inflammatory cytokine (IL-10) significantly, whereas relatively high dose was required to alter the IL-6 and TGF-β expression. Overall, the present study revealed that BG extracted from HII31 cells alters the expression of studied cytokines, which can be used as a potent immunomodulator in pharmaceutical products. © 2017, Sociedade Brasileira de Ciencia e Tecnologia de Alimentos, SBCTA. All rights reserved.


Chaiyasut C.,Chiang Mai University | Sivamaruthi B.S.,Chiang Mai University | Peerajan S.,Health Innovation Institute | Sirilun S.,Chiang Mai University | And 2 more authors.
International Food Research Journal | Year: 2017

Fermented plant beverages (FPB) are produced from cereals, fruits, vegetables and other plant sources. This study aimed to assess the heavy metals, minerals, alcohol, and fusel oil content of seventy FPBs sampled from several parts of Thailand. The results indicated that tested FPBs products were harmless for consumption except some of the products that are made from Morinda citrifolia Linn. The iron content of few products was found higher owing to the use of underground water for the production, which can be nullified by controlled consumption of FPBs. About 21 products are not suitable for consumption as per Thai community product standards (TCPS). All the samples were scored based on Australia New Zealand Food Authority standards (ANZFA), TCPS and Thai industrial standards-2089. The outcome of current study has revealed the importance of selection of raw materials for FPBs and also determined the quality of the selected FPB in Thailand. © All Rights Reserved.


Pengkumsri N.,Chiang Mai University | Suwannalert P.,Mahidol University | Sivamaruthi B.S.,Chiang Mai University | Wongpoomchai R.,Chiang Mai University | And 6 more authors.
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2015

Objective: The current study was conceived and performed to assess the pathophysiological, histological, and molecular manipulations of dextran sodium sulphate (DSS; MW: 5,000 Da) intervention in the rat and determined the changes in the antioxidant capacity of host and representative antioxidant enzymes. Methods: Wistar rats were fed with two different concentrations (3 and 5%) of DSS for seven days and caged for another seven days. Then colon and serum samples were collected, and colitis induction was assessed by histochemical examination. The level of antioxidant enzymes were determined by spectroscopy methods, and gene regulations were evaluated by qPCR. Results: The body mass of rat was gradually reduced to DSS intervention compared to naive control. The statistically significant level of reduction in the colon length has been recorded in DSS-treated rats (3% DSS-treated: 14.33±0.53 cm; 5% DSS-treated: 13.73±0.53 cm) compared to control (Control: 17.41±0.54 cm). The total histological scores of different study groups suggested that DSS causes the significant level of damages in rat colon. The antioxidant capacity of the host was significantly reduced in terms of trolox equivalence. About three-fold higher the amount of malondialdehyde was recorded in 5% DSS-treated group compared to control. The content of antioxidant enzymes were drastically reduced (1.4-2.7 fold) upon DSS exposure than naïve control. The expression of selected inflammatory markers (IL-6, TNF-α, and iNOS) was up-regulated in DSS-exposed groups. Conclusion: The current study clearly indicated that DSS altered the expression of selected inflammatory genes, antioxidant capacity, and scavenging enzymes in such a way that it facilitates the development of colitis in Wistar rat and the study provides the necessary information the experimental designing to explore the ability of any active principle against colitis using DSS (5 KDa) induced colitis rat model. © 2015, International Journal of Pharmacy and Pharmaceutical Science. All rights reserved.


Sirilun S.,Chiang Mai University | Chaiyasut C.,Chiang Mai University | Sivamaruthi B.S.,Chiang Mai University | Peerajan S.,Health Innovation Institute | And 2 more authors.
Asian Journal of Pharmaceutical and Clinical Research | Year: 2017

Objective: Preservatives are used in the cosmetic products to protect the potential growth of microbes, therefore, to prolong the shelf life of products, and to protect the consumer from infections. However, several preservatives can cause various health problems, and the safety profiles of those preservatives are still unclear. Many natural substances are used in the cosmetic products to substitute the traditional preservatives. The present study deals with the evaluation of conservative nature of phenethyl alcohol (PEA) in three cosmetic formulations (emulsion, cleansing, and conditioner). Methods: Three different concentrations of PEA (0.3%, 1%, and 2.5%) were used in cosmetic formulations. The physical appearance of the formulas was assessed manually, and the antimicrobial nature of PEA and PEA-containing cosmetic formulations was evaluated by agar well plate assay. Results: The use of PEA has not affected the physical appearance and quality of the formulations, except the high concentration of PEA in the cleansing solution, which reduced the foam formation. The minimal required concentration of PEA in emulsions and cleansings was 1.0% and 2.5% in the conditioners. All cosmetic preparations were subjected to antibacterial and antifungal evaluation. The alkaline pH (>8) affected the antimicrobial activity of PEA in a cosmetic product. Conclusion: The results suggested that PEA is a potent non-traditional preservative for the cosmetic formulations, especially, in the emulsion, cleansing solutions, and conditioners. © 2017 The Authors.


Chaiyasut C.,Chiang Mai University | Pattananandecha T.,Chiang Mai University | Sirilun S.,Chiang Mai University | Suwannalert P.,Mahidol University | And 2 more authors.
Food Science and Technology | Year: 2017

Host microbiome and metabolome are associated with the incidence of colorectal cancer (CC), one of the major health problems in developed countries. The pro and prebiotic supplementation helps to improve the host health. Inulin is one such prebiotic used for the enhancement of naïve probiotic bacterial population. This paper explains the impact of inulin (PRE) extracted from Jerusalem artichoke (JA), Lactobacillus plantarum HII11 (PRO), and synbiotic (SYN; inulin + L. plantarum HII11) preparation on Azoxymethane mediated CC induced rat model with respect to changes in microbial load, microbial enzymes, and preneoplastic aberrant crypt foci. The results suggested that the PRE and SYN supplementation effectively reduced the selected pathogenic bacteria (Salmonella spp., and Escherichia coli), microbial enzymes and increased the probiotic load. The intervention of SYN significantly reduced the colonic ACF in CC model. The study results revealed that the supplementation of SYN diet (inulin and L. plantarum HII11) protects the AOM-mediated colon cancer induced host. © 2017, Sociedade Brasileira de Ciencia e Tecnologia de Alimentos, SBCTA. All rights reserved.


Chaiyasut C.,Chiang Mai University | Pengkumsri N.,Chiang Mai University | Sirilun S.,Chiang Mai University | Peerajan S.,Health Innovation Institute | And 2 more authors.
AMB Express | Year: 2017

Studies on phytochemical properties and bioactivities of rice bran revealed the wealth of natural complex antioxidant compounds. The composition and the properties of the rice bran get altered after fermentation by several microbes. This study was designed to optimize the black rice bran fermentation conditions for the total anthocyanin (ACN) content, total antioxidant properties, and relative activity of β-glucosidase (BGS) by Saccharomyces cerevisiae. The Box–Behnken design and response surface methodology was employed to achieve the maximum response in fermentation. The kinetic analysis of HPLC based phytochemical determination and bioconversion of ACN, and in vitro antioxidant assays were performed during fermentation. The optimum pH, temperature and NaCl concentration to achieve maximum ACN content, antioxidant capacity, and BGS activity were pH 4.0, 40 °C, and 0.5%, respectively. Bioconversion of cyanidin-3-glucoside and peonidin-3-glucoside to cyanidin and peonidin was recorded at a significant level, respectively. The maximum activity of BGS on rice bran was noticed at 24 h of fermentation. The results suggested that phytochemical content was not changed significantly, whereas the antioxidant properties of rice bran were slightly enhanced after 24 h of fermentation. Additional detailed in vivo evaluation is required to explain the impact of submerged fermentation on the bioactivity of rice bran. © 2017, The Author(s).


Duangjitcharoen Y.,Chiang Mai University | Kantachote D.,Prince of Songkla University | Prasitpuripreecha C.,Ubon Ratchathani University | Peerajan S.,Health Innovation Institute | Chaiyasut C.,Chiang Mai University
Journal of Applied Pharmaceutical Science | Year: 2014

Potential probiotic strain for being health protectant especially intestinal illness is strain specific. This study investigated the selection of a new strain of probiotic of non-human origin and of human origin with the properties of intestinal protection against cancer. From the primary screening results, the human feces origin strains showed more bile salt tolerance than the fermented food origin strains. Whereas none of the human feces origin isolates could grow well in the acid condition. Lactobacillus plantarum CM4 was the new probiotic of non-human origin strain for this study. CM4 cells is said to tolerate and grow in 0.3% bile salt after 5 hours of incubation, at pH3 after 6 hours of incubation. This is in agreement with in vivo study for intestinal adherence ability of probiotic, a live CM4 cells was able to persist in mice small intestine and colon for 5 days. Live CM4 cells showed most effectiveness to bind 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) mutagen after 24 hours of incubation with 46.32% of binding ability while 144 hours of incubation with 85.34% of binding ability was the most effective for 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) mutagen. The significant difference (p<0.05) was found at all those time points. Moreover, the CM4 strain could degrade diphenylnitrosamine (DPN) better than 1-nitrosopyrrolidine (NPR) with dose response relationship activity. These imply that the CM4 strain could be the value added for the consuming pharmaceutical probiotic product based on scientific proof of its role in intestinal survival properties and cancer prevention through binding PhIP and IQ mutagen as well as degrading nitrosamine. © 2014 Yodsawee Duangjitcharoen et al.


Woraharn S.,Chiang Mai University | Lailerd N.,Chiang Mai University | Sivamaruthi B.S.,Chiang Mai University | Wangcharoen W.,Maejo University | And 3 more authors.
International Journal of Food Science and Technology | Year: 2015

Fermented plant beverages (FPB) with a high content of desirable principle components are served as functional foods from several years. Hericium erinaceus is famous for its antimicrobial, antioxidant, antihypertensive and antidiabetic nature. Accordingly, the current study was aimed to produce fermented H. erinaceus juice with a high content of L-glutamine (Gln) and L-glutamic acid (GA) through lactic acid bacteria (LAB) isolated from fermented Thai foods. LAB isolates were screened and identified the potent protease-producing bacteria Enterococcus faecalis (G414/1) that facilitate the production of Gln and GA through protein hydrolysis. Box-Behnken design (BBD) and response surface methodology (RSM) were adapted for the optimisation of conditions for the increased production of Gln and GA during fermentation of H. erinaceus. We succeeded with an optimum concentration of cofactor (CaCl2), pH and temperature for improved protease activity and subsequent Gln and GA production. The ability of isolated E. faecalis strain to produce Gln and GA was demonstrated in this study. Further, upstream processes like strain improvement and media optimisation will direct the way to produce enriched H. erinaceus based FPB. © 2015 Institute of Food Science and Technology.


Pattananandecha T.,Chiang Mai University | Sirilun S.,Chiang Mai University | Duangjitcharoen Y.,Chiang Mai University | Sivamaruthi B.S.,Chiang Mai University | And 3 more authors.
Pharmaceutical Biology | Year: 2016

Context Inulin, a non-digestible carbohydrate isolated from Helianthus tuberosus L. (Asteraceae), has been shown to alter the gut beneficial bacteria including Lactobacillus spp. and Bifidobacteria. Inulin also influences the activities of intestinal microbiota that could prevent the colon cancer development. Objective This study determines the effect of hydrolysed inulin with different degrees of polymerisation on alteration of intestinal microbiota and their activities on azoxymethane (AOM)-induced preneoplastic aberrant crypt foci (ACF) in rats. Materials and methods Seventy-two male Sprague–Dawley rats were randomly divided into six groups (three control and three AOM-treated groups) and the animal were fed with either a normal diet or diet containing 10% of long-chain inulin (InuL) or short-chain inulin (InuS), respectively, for 17 weeks. Colon cancer was induced in rats by injecting AOM subcutaneously at the 8th and 9th week of the study period. At the end of the experiment, cecal contents of rats were examined for selected microbiota, organic acids, putrefactive compounds and microbial enzymes. ACF formation was microscopically examined. Results The inulin diets significantly increased the weight and decreased the pH of the caecal content. The rats fed with InuL-supplemented diet showed approximately 2.9- and 6.8-fold increases in the biomass of Lactobacillus spp. and Bifidobacteria, respectively. Naive and AOM-treated rats fed with inulin-supplemented diet showed ∼1.3- and ∼2.2-fold decreases in the biomass of Escherichia coli and Salmonella enterica serovar Typhi, respectively. Inulins significantly decreased the colonic concentration of phenol, p-cresol and indole. Reduction in the activity of microbial enzymes such as β-glucuronidase, azoreductase and nitroreductase were observed in inulin-treated animals. Reduction in the ACF formation has been observed in inulin-treated groups. Discussion and conclusion The present study demonstrates that dietary administration of inulin reduces the formation of preneoplastic lesions in the colon, possibly by altering the microecology and microbial activities on carcinogenesis. © 2016 Taylor & Francis


Yamamoto S.,Yamaguchi University | Pattananandecha T.,Chiang Mai University | Sirilun S.,Chiang Mai University | Sivamaruthi B.S.,Chiang Mai University | And 2 more authors.
Journal of Pure and Applied Microbiology | Year: 2016

The preservation of synbiotic preparations is the most important task than the formulation, due to the stability issues. Many advanced techniques are employed to preserve and stabilize the viability of synbiotic formulas. The current study deals with the development of freeze-dried (FD) Lactobacillus paracasei HII01 with selected commercial inulins (inulin GR, inulin SC, inulin FF), inulin extracted from Jerusalem artichoke (inulin JA), skim milk, maltodextrin #2, trehalose, and sorbitol as cryoprotectants and assessment of viability during intestinal transit and storage. The trehalose, inulin F F, and inulin JA retain the survivability of L. paracasei HII01 up to 46.1-79.5%, 19.0-51.0%, and 10.3-33.1%, respectively, whereas, the sorbitol showed least survival rate (1.4-12.7%) of L. paracasei HII01 and the FD-L. paracasei HII01 without any cryoprotectants had no viable cells. The FD-powders with the high survival of L. paracasei HII01 were selected to study the viability during gastrointestinal transit (Simulated gastric fluid, and simulated intestinal fluid of human). The FD-L. paracasei HII01 with inulins protects the survivability of L. paracasei HII01 during gastrointestinal transit more than other studied protectants. The results suggested that the storage of FD-L. paracasei HII01 with inulin JA at 4°C can retain ∼59.2% of the existence of live L. paracasei HII01 cells for 60 days than other tested commercially available inulins, while the samples stored at room temperature showed the drastic reduction in the viability. Collectively, the results suggested that the inulin JA can be used in freeze drying process of L. paracasei HII01 as a cryoprotectant, which effectively diminishes the degradation of probiotic cells during gastrointestinal transit and storage.

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