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Park D.W.,Health and Environment Research Institute of Gwangju | Park D.W.,Chonnam National University | Kim S.-H.,Health and Environment Research Institute of Gwangju | Park J.W.,Health and Environment Research Institute of Gwangju | And 13 more authors.
Emerging Infectious Diseases | Year: 2017

The incidence rate for scarlet fever in South Korea is ris­ing. During 2008–2015, we collected group A Streptococ­cus isolates and performed emm and exotoxin genotyping and disk-diffusion antimicrobial tests. Scarlet fever in South Korea was most closely associated with emm types emm4, emm28, emm1, and emm3. In 2015, tetracycline resistance started increasing. © 2017, Centers for Disease Control and Prevention (CDC). All Rights Reserved.


Kim D.,Chonnam National University | Lee H.-M.,Chonnam National University | Lee H.-M.,Korea Basic Science Institute | Oh K.-S.,Chonnam National University | And 12 more authors.
Biomaterials | Year: 2017

Rapid methods for the detection and clinical treatment of human norovirus (HuNoV) are needed to control foodborne disease outbreaks, but reliable techniques that are fast and sensitive enough to detect small amounts of HuNoV in food and aquatic environments are not yet available. We explore the interactions between HuNoV and concanavalin A (Con A), which could facilitate the development of a sensitive detection tool for HuNoV. Biophysical studies including hydrogen/deuterium exchange (HDX) mass spectrometry and surface plasmon resonance (SPR) revealed that when the metal coordinated region of Con A, which spans Asp16 to His24, is converted to nine alanine residues (mCon AMCR), the affinity for HuNoV (GII.4) diminishes, demonstrating that this Ca2+ and Mn2+ coordinated region is responsible for the observed virus-protein interaction. The mutated carbohydrate binding region of Con A (mCon ACBR) does not affect binding affinity significantly, indicating that MCR of Con A is a major region of interaction to HuNoV (GII.4). The results further contribute to the development of a HuNoV concentration tool, Con A-immobilized polyacrylate beads (Con A-PAB), for rapid detection of genotypes from genogroups I and II (GI and GII). This method offers many advantages over currently available methods, including a short concentration time. HuNov (GI and GII) can be detected in just 15 min with 90% recovery through Con A-PAB application. In addition, this method can be used over a wide range of pH values (pH 3.0 – 10.0). Overall, this rapid and sensitive detection of HuNoV (GI and GII) will aid in the prevention of virus transmission pathways, and the method developed here may have applicability for other foodborne viral infections. © 2017 Elsevier Ltd


Kim J.-P.,Health and Environment Research Institute of Gwangju | Yang Y.-S.,Health and Environment Research Institute of Gwangju | Kim J.-H.,Health and Environment Research Institute of Gwangju | Lee H.-H.,Health and Environment Research Institute of Gwangju | And 4 more authors.
Korean Journal of Food Science and Technology | Year: 2012

We investigated the chemical properties and antioxidant activities of sword bean (SWB) and compared it to soybean (SB) and black soybean (seoritae, BSB). The value of vitamin C, vitamin A, crude fat, and crude protein in SWB was 25.5, 0.37 mg/kg, 1.2, and 25.6%, respectively. The crude fat content (1.2%) in SWB was very low in comparison with those of SB (16.5%) and BSB (16.1%). In 16 free amino acids investigated, the histidine content (9.2%) was high in SWB, followed by SB (3.0%) and BSB (2.9%). Total flavonoid content of SWB (493.2 mg/100 g) was significantly higher than those of SB (71.8 mg/100 g) and BSB (97.5 mg/100 g). Total polyphenol content of SWB (1,152.0 mg/100 g) was not significantly different from that of SB (1,165.7 mg/100 g) but lower than that of BSB (1,298.6 mg/100 g). DPPH radical scavenging activity (SC50, 50% scavenging concentration) of SWB was 13.1 μg/mL, whereas that of positive control (α-tocopherol) was 8.3 μg/mL. © The Korean Society of Food Science and Technology.


Kim T.Y.,Chonnam National University | An S.S.,Health and Environment Research Institute of Gwangju | Shim W.G.,Chonnam National University | Lee J.W.,Chosun University | And 2 more authors.
Journal of Industrial and Engineering Chemistry | Year: 2015

The adsorption and energetic heterogeneity properties of cesium ion on ion exchange resin were investigated. The adsorption equilibrium isotherm of cesium ion on ion exchange resin could be represented by the Lamgmuir equation. Thermodynamic parameters indicated that adsorption of cesium ion on ion exchange resin was spontaneous and endothermic. Adsorption heterogeneity for the adsorption system of cesium ions on Amberlite IR 120 increased with increasing temperature and decreasing pH. A model based on linear driving force approximation was used for simulating the adsorption behavior of cesium ions in a fixed bed adsorber. © 2015 The Korean Society of Industrial and Engineering Chemistry.


Kim M.J.,Health and Environment Research Institute of Gwangju | Jeong H.S.,National Health Research Institute | Kim S.G.,Health and Environment Research Institute of Gwangju | Lee S.M.,Health and Environment Research Institute of Gwangju | And 8 more authors.
Osong Public Health and Research Perspectives | Year: 2014

Objectives: The introduction of new rotavirus vaccines into the public sphere makes it necessary to maintain constant surveillance and to heighten public awareness of the appearance of new rotavirus strains. We describe the molecular epidemiology of circulating rotavirus strains after vaccine introduction. Methods: We collected a total of 1070 stool samples from children with gastroenteritis from January 2013 to June 2013. The antigenic prevalence of rotavirus group A was distinguished using enzyme immunoassay. The G and P genotypes of enzyme immunoassay-positive samples were determined with reverse transcription-polymerase chain reaction and nucleotide sequencing analysis. Results: Of the 1070 samples collected, 277 (25.9%) tested positive for rotaviruses by enzyme-linked immunoabsorbent assay. The most prevalent circulating genotype G was G1 (51.3%), followed by G2 (34.7%) and G9 (10.8%). The predominant type of genotype P was P[8] (66.1%), followed by P[4] (31.4%). In this study, nine genotypes were found. G1P[8] was the most prevalent (51.8%), followed by G2P[4] (30.5%), G9P[8] (9.9%), and G2P[8] (4.0%). Several unusual combinations (G1P[4], G3P[9], G3P[8], G4P[6], and G9P[4]) were also identified. Conclusion: Molecular epidemiological knowledge of rotaviruses is critical for the development of effective preventive measures, including vaccines. These data will help us monitor the effectiveness of current rotavirus vaccines. © 2014.


Lim M.S.,Chosun University | Kim S.H.,Health and Environment Research Institute of Gwangju | Chun Y.N.,Chosun University
Applied Chemistry for Engineering | Year: 2015

CCU (Carbon Capture & Utilization) has a potential technology for the reduction and usage of carbon dioxide which is greenhouse gas emitting from a fossil fuel buring. To decompose the carbon dioxide, a three phase gliding arc plasma-catalytic reactor was designed and manufactured. Experiments of carbon dioxide reduction was performed by varying the gas flow rate with feeding the CO2 only as well as the input power, the catalyst type and steam supply with respect to the injection of the mixture of CO2 and CH4. The CO2 decomposition rate was 7.9% and the energy efficiency was 0.0013 L/min⋅W at a CO2 flow rate of 12 L/min only. Carbon monoxide and oxygen was generated in accordance with the destruction of carbon dioxide. When the injection ratio of CH4/CO2 reached 1.29, the CO2 destruction and CH4 conversion rates were 37.8% and 56.6% respectively at a power supply of 0.76 kW. During the installation of NiO/Al2O3 catalyst bed, the CO2 destruction and CH4 conversion rates were 11.5% and 9.9% respectively. The steam supply parameter do not have any significant effects on the carbon dioxide decomposition. © Korean Society of Industrial Engineering Chemistry. All rights reserved.


Kim K.-G.,Health and Environment Research Institute of Gwangju | Park D.-W.,Health and Environment Research Institute of Gwangju | Kang G.-R.,Health and Environment Research Institute of Gwangju | Kim T.-S.,Health and Environment Research Institute of Gwangju | And 6 more authors.
Food Chemistry | Year: 2016

A simple and sensitive analytical method based on QuEChERS approach using liquid chromatography tandem mass spectrometry was developed and validated for the determination of 6-benzylaminopurine, carbendazim and thiabendazole in bean sprouts. Sodium chloride and sodium acetate were used for salting-out step and magnesium sulfate for clean-up. The validation of optimized method was satisfactory with recoveries, between 89.5% and 103.2% for the three compounds, and relative standard deviation (RSD) values less than 3.3% at 20 and 40 ng/g fortification levels (n = 5). Limit of detection (LOD) and limit of quantification (LOQ) was 2.1-3.7 ng/g and 6.3-11.1 ng/g, respectively. Monitoring of 126 bean sprout samples collected from local markets was performed to verify the adaptability in real samples. No pesticides were detected but 6-benzylaminopurine was found in 3 samples at the level of 15-20 ng/g. The optimized method should be applicable for monitoring of 6-benzylaminopurine, carbendazim and thiabendazole in bean sprouts in short time. © 2016 Elsevier Ltd. All rights reserved.


Park D.W.,Health and Environment Research Institute of Gwangju | Kim K.G.,Health and Environment Research Institute of Gwangju | Choi E.A.,Health and Environment Research Institute of Gwangju | Kang G.R.,Health and Environment Research Institute of Gwangju | And 6 more authors.
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2015

South Korea has a unique food culture. South Koreans enjoy wrapping meat and eating or making kimchi (traditionally fermented Korean food) and eating using raw leafy vegetables, stalk and stem vegetables. Therefore, there is a high chance of being exposed to pesticide residues of vegetables. The objective of this study was to investigate pesticide residues in leafy vegetables, stalk and stem vegetables from South Korea. A total of 8496 samples were mainly collected from Gwangju and Jeonnam area (the largest production region of leafy vegetables, stalk and stem vegetables) in South Korea from 2010 to 2014. A total of 230 pesticides were used for multi-residue analysis of pesticides. Among 8496 samples, 61 different pesticides (1029 times) were detected in 890 samples, of which 118 samples (1.4%) exceeded the Korea maximum residue limits (MRLs). Samples exceeding the MRLs were mostly found in leafy vegetables (brassica lee ssp. namai, leafy lettuce, spinach, perilla leaves, crown daisy, marsh mallow, aster scaber, pimpinella brachycarpa) and Chinese chive. Procymidone, dimethomorph and azoxystrobin were the most frequently found pesticides. A risk assessment of pesticides exceeding the MRLs was evaluated by calculating the estimated daily intake (EDI) and the acceptable daily intake (ADI). The ratio of EDI to ADI was 0.003–30.4%. © 2015 Health and Environment research Institute of Gwangju, Korea. Published by Taylor & Francis.


PubMed | Health and Environment Research Institute of Gwangju
Type: Journal Article | Journal: Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment | Year: 2015

South Korea has a unique food culture. South Koreans enjoy wrapping meat and eating or making kimchi (traditionally fermented Korean food) and eating using raw leafy vegetables, stalk and stem vegetables. Therefore, there is a high chance of being exposed to pesticide residues of vegetables. The objective of this study was to investigate pesticide residues in leafy vegetables, stalk and stem vegetables from South Korea. A total of 8496 samples were mainly collected from Gwangju and Jeonnam area (the largest production region of leafy vegetables, stalk and stem vegetables) in South Korea from 2010 to 2014. A total of 230 pesticides were used for multi-residue analysis of pesticides. Among 8496 samples, 61 different pesticides (1029 times) were detected in 890 samples, of which 118 samples (1.4%) exceeded the Korea maximum residue limits (MRLs). Samples exceeding the MRLs were mostly found in leafy vegetables (brassica lee ssp. namai, leafy lettuce, spinach, perilla leaves, crown daisy, marsh mallow, aster scaber, pimpinella brachycarpa) and Chinese chive. Procymidone, dimethomorph and azoxystrobin were the most frequently found pesticides. A risk assessment of pesticides exceeding the MRLs was evaluated by calculating the estimated daily intake (EDI) and the acceptable daily intake (ADI). The ratio of EDI to ADI was 0.003-30.4%.


PubMed | Health and Environment Research Institute of Gwangju
Type: | Journal: Food chemistry | Year: 2016

A simple and sensitive analytical method based on QuEChERS approach using liquid chromatography tandem mass spectrometry was developed and validated for the determination of 6-benzylaminopurine, carbendazim and thiabendazole in bean sprouts. Sodium chloride and sodium acetate were used for salting-out step and magnesium sulfate for clean-up. The validation of optimized method was satisfactory with recoveries, between 89.5% and 103.2% for the three compounds, and relative standard deviation (RSD) values less than 3.3% at 20 and 40ng/g fortification levels (n=5). Limit of detection (LOD) and limit of quantification (LOQ) was 2.1-3.7ng/g and 6.3-11.1ng/g, respectively. Monitoring of 126 bean sprout samples collected from local markets was performed to verify the adaptability in real samples. No pesticides were detected but 6-benzylaminopurine was found in 3 samples at the level of 15-20ng/g. The optimized method should be applicable for monitoring of 6-benzylaminopurine, carbendazim and thiabendazole in bean sprouts in short time.

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