Gwangju Institute of Health and Environment

Gwangju, South Korea

Gwangju Institute of Health and Environment

Gwangju, South Korea
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Rhee J.E.,National Institute of Health | Shin M.Y.,Jeollanam Do Institute of Health and Environment | Kim C.M.,Jeollanam Do Institute of Health and Environment | Kee H.Y.,Gwangju Institute of Health and Environment | And 6 more authors.
Virology Journal | Year: 2010

We investigated the prevalence of human papillomavirus (HPV) infection and the distribution of high-risk HPV genotypes among 2,308 high-risk Korean women to predict how much the current prophylactic HPV vaccines might affect the prevention of cervical cancer in Korea. HPV DNA was detected in 939 women (40.7%) but only one-third of women were positive for HPV-16 and/or HPV-18, the genotypes used for developing the HPV vaccines. Thus, the development of area-specific HPV vaccines based on dominant HPV genotypes in our country is needed for preventing HPV infection and the development of premalignant lesions in the cervix of Korean women. © 2010 Rhee et al; licensee BioMed Central Ltd.


Tamang M.D.,National Veterinary Research and Quarantine Service | Nam H.-M.,National Veterinary Research and Quarantine Service | Kim A.,National Veterinary Research and Quarantine Service | Lee H.-S.,National Veterinary Research and Quarantine Service | And 5 more authors.
Foodborne Pathogens and Disease | Year: 2011

The aim of this study was to investigate the prevalence and mechanism of quinolone resistance among selected nontyphoid Salmonella (NTS) isolates. A total of 1279 NTS isolated from food animals (n=692) and humans (n=587) between 1995 and 2009 were investigated by serotyping, antimicrobial susceptibility testing, screening for plasmid-mediated quinolone resistance (PMQR) genes qnr, aac(6′)-Ib-cr, and qepA and mutations in the quinolone resistance-determining region (QRDR) of gyrA and parC by PCR, and DNA sequencing. Three hundred thirty (47.7%) of 692 animal isolates and 177 (30.2%) of 587 human isolates were resistant to nalidixic acid. Most animal (94.8%, 313/330) and human (99.4%, 176/177) NTS exhibited decreased ciprofloxacin susceptibility (minimum inhibitory concentration [MIC]: 0.125-2mg/L). None of them carried qnr or qepA gene. However, aac(6′)-Ib was identified in six animal isolates, of which four carried aac(6′)-Ib-cr gene. Based on antimicrobial resistance profile, year of isolation, MIC for quinolones and fluoroquinolones, and isolation frequency of serotype, 114 animal and 83 human isolates were tested for QRDR mutations. All contained a single mutation within the QRDR of gyrA at either codon 87 or 83, and 41 of them contained an additional mutation in parC. The most prevalent mutation was Asp87-Tyr (n=107), followed by Asp87-Gly (n=28), Asp87-Asn (n=26), Ser83-Tyr (n=22), and Ser83-Phe (n=14). Point mutations in parC were observed outside the QRDR, which included 40 isolates with Thr57-Ser substitution and 1 Salmonella Typhimurium with a novel Glu51-Lys substitution. In conclusion, a point mutation within the QRDR of gyrA was primarily responsible for quinolone resistance and reduced susceptibility to fluoroquinolones in NTS in Korea. To our knowledge, this is the first report of occurrence of aac(6′)-Ib-cr gene among NTS in Korea. The spread of NTS carrying aac(6′)-Ib-cr is of serious concern and should be carefully monitored. © 2011, Mary Ann Liebert, Inc.


Tamang M.D.,National Veterinary Research and Quarantine Service | Nam H.-M.,National Veterinary Research and Quarantine Service | Kim T.-S.,Gwangju Institute of Health and Environment | Jang G.-C.,National Veterinary Research and Quarantine Service | And 2 more authors.
Journal of Clinical Microbiology | Year: 2011

Twenty of 1,279 nontyphoid Salmonella strains isolated from food animals and humans produced CTX-Mtype extended-spectrum β-lactamase. All expressed CTX-M-15, except two which coexpressed CTX-M-14 and TEM-1. Insertion sequence ISEcp1 was identified upstream of blaCTX-M genes. The bla CTX-M-15 and blaCTX-M-14 genes were disseminated by large conjugative IncFIIs and IncI1-Iβ plasmids, respectively. Copyright © 2011, American Society for Microbiology. All Rights Reserved.

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