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Bucharest, Romania

Stoica P.,Science ICPE Bistrita S.A | Stoica P.,University of Bucharest | Chifiriuc M.C.,University of Bucharest | Rapa M.,Science ICPE Bistrita S.A | And 9 more authors.
Romanian Biotechnological Letters

The continuously spreading use of implantable medical devices (IMDs) in all fields of medicine increased the risk of microbial colonization leading to difficult to treat infections. The objective of this study was to obtain and characterize by physico-chemical and biological methods, new biopolymeric composites with anti-adherence properties based on polycaprolactone (PCL) dopped with chitosan (CS) and essential oils (EOs) as antimicrobial agents, for further use in the manufacture or coating of IMDs. The EOs were extracted from cinnamon (CIN), coriander (COR) and tea tree (TEA), and were characterized by GC-MS. Biopolymeric composite films (PCL/CS, PCL/CS/EOs) were obtained by solvent evaporation in two variants and further characterized by DSC and FTIR techniques. The in vitro antimicrobial activity of EOs was determined by the minimum inhibitory concentration (MIC) assay in liquid medium, towards Gram-positive and Gram-negative bacterial strains. The anti-adherence activity of films was analyzed against Staphylococcus aureus ATCC 29213 by viable cell counts assay. The cytotoxicity of the obtained films was assessed using the HT29 cell line. The results of this study suggest that CIN EO strongly inhibited the growth of all tested bacterial species, followed by TEA and COR EOs. From all tested composites, only PCL/CS/CIN1 exhibited a significant anti-adherence effect. © 2015 university of bucharest. Source

Stoica P.,Science ICPE Bistrita S.A | Stoica P.,University of Bucharest | Rapa M.,Science ICPE Bistrita S.A | Chifiriuc M.-C.,Science ICPE Bistrita S.A | And 9 more authors.
Romanian Biotechnological Letters

Novel biocompatible and antimicrobial composites based on poly(lactic acid) (PLA), hydrolyzed collagen (HC) and silver nanoparticles (AgNPs) were prepared by melt processing. Tributyl o-acetyl citrate (ATBC) was used as plasticizer, for improving the processability of PLA. The influence of HC and AgNPs on the PLA bionanocomposites was investigated in terms of biocompatibility, antifungal activity and water contact angle measurements. Surface morphology by SEM and the identification of AgNPs by UV-Vis were also presented. The investigated bionanocomposites exhibited the characteristic plasmonic effect of silver nanoparticles. All composites showed a high degree of biocompatibility. Sample containing HC 5 wt.% and AgNPs showed a significant antifungal property, inhibiting fungal adhesion and mature biofilm development. The increased hydrophilicity determined by contact angle analysis for the PLA/HC10/AgNPs composite did no contributed significantly to improving of antiadherence effect. 10 wt.% HC promoted fungal colonization and mature biofilm development. Source

Coculescu B.-I.,Titu Maiorescu University | Palade A.-M.,National Institute Of Res Dev For Microbiology And Immunology Cantacuzino Nirdmic | Delcaru C.,National Institute Of Res Dev For Microbiology And Immunology Cantacuzino Nirdmic | Pircalobioru G.,University College Dublin | And 2 more authors.
Romanian Biotechnological Letters

The purpose of this study was to screen the genotype of antibiotic resistance markers of a collection of 18 Salmonella Typhimurium strains isolated from children with gastrointestinal disorders admitted to a county hospital pediatrics. 16 of the 18 clinical isolates of S. Typhimurium (88.89%) showed the phenomenon of multiresistance to antibiotics (MDR), at the same time being resistant to betalactams, aminoglycosides, nalidixic acid, tetracycline and sulfonamides. Extended-spectrum beta-lactamases (ESBLs) production was detected by the double disk synergy test (DDST). The 16 strains showed ESBLs phenotype associated with phenotypes of resistance to aminoglycosides, nalidixic acid, cotrimoxazole (trimethoprim/sulfamethoxazole) and tetracycline, and of these, seven showed ESBLs phenotype associated with chloramphenicol resistance phenotype. Molecular analysis of the 18 strains of S. Typhimurium with multiple resistance to antibiotics revealed three PCR resistance profiles: blaSHV+, blaSHV+ blaTEM+, and blaSHV+ blaTEM+ blaCTX-M+. Out of the 18 strains of S. Typhimurium, 88.89% were blaTEM gene carriers, 100% blaSHV gene, and the 16 strains were positive for both genes (88.89%). A single strain introduced blaCTX-M gene in combination with the other two genes (5.56%). © 2015 university of bucharest. Source

Stetiu A.A.,Lucian Blaga University | Oleksik V.,Lucian Blaga University | Stetiu M.,Lucian Blaga University | Burlibasa M.,Carol Davila University of Medicine and Pharmacy | And 5 more authors.
Romanian Biotechnological Letters

Our aim is to provide to the researchers with a method for applying finite element analysis in dentistry. It is well known that dentistry has evolved and more prosthetic reconstructions are made using CAD-CAM technology. Thus, is necessary to investigate how the stress and deformations in this works are acting and if there are some possibilities to improve the shapes of the preparations. Starting point was 3D scanning of the dental casts and natural teeth as well. 3D modelling stage for teeth reconstruction is presented. As conclusion a finite element analyse of natural teeth is presented. The FEM provide us information about stress and strains, principal stress and principal strains, nodal displacements and safety factor. Source

Chatziavramidis A.,Head and Neck Surgery Clinic | Kondylidou-Sidira A.,Aristotle University of Thessaloniki | Stefanidis A.,Theagenion Cancer Hospital | Soldatou S.,Theagenion Cancer Hospital
BMJ Case Reports

Rhinoliths consist of a central nidus with calcification resulting in calcareous concretions within the nasal cavity. They are uncommon in the literature despite a propensity particularly in children to insert foreign bodies into their nose and ears. We present the case of a 62-year-old woman with a longstanding undetected rhinolith with mild uncharacteristic symptoms. Radiographic examination revealed anatomical alteration of the inferior turbinate that was attributed to the long presence of the rhinolith in the nasal cavity. The management of the rhinolith and a review of the literature are presented. Source

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