Erfatpour M.,Islamic Azad University at Tehran |
Hamidogli Y.,Guilan University |
Kaviani B.,Islamic Azad University at Rasht |
Fatahi R.,University of Tehran |
And 3 more authors.
Australian Journal of Crop Science | Year: 2011
Studying genetic diversity is a preliminary step for conservation and utilization of germplasm. Talesh hills, located in Guilan province in northern part of Iran, are one of the main areas for natural distribution of hazelnut (Corylus avellana L.). Hazelnut trees in Talesh Mountains are a wild population (land race) and obtained from open-pollinated seedling. In this research, a population including 90 hazelnut samples was studied using 15 microsatellite markers. Totally, 98 polymorphic alleles were detected with an average of 6.53 alleles per locus. The lowest numbers of 3 alleles were observed in CaC-B005 and CaC-C114 and the highest number of 12 alleles in CaC-C101 locus. The average effective number of alleles (3.75), mean expected heterozygosity of (He=0.70) and average observed heterozygosity of (Ho=0.62) were estimated for studied loci. All loci showed high PIC with an average of 0.72 that the highest amount detected in CaC-B011 and CaC-B101 loci with values of 0.85 and 0.84, respectively. A dendrogram was generated by UPGMA clustering method that placed the 90 accessions into 7 main loose groups. According to the results a high genetic diversity was found in the studied population that mainly related to the hazelnut pollination nature that shows sporophytic incompatibility system enhancing out-crossing in natural populations.