Kallianpur K.J.,Hawaii Center for AIDS |
Umaki T.M.,Hawaii Center for AIDS |
Ndhlovu L.C.,Hawaii Center for AIDS |
Nakamoto B.K.,Hawaii Center for AIDS |
And 3 more authors.
Mitochondrion | Year: 2016
Cross-sectional relationships were examined between regional brain volumes and mitochondrial DNA (mtDNA) 8-hydroxy-2-deoxyguanosine (8-oxo-dG) in peripheral blood mononuclear cells (PBMCs) of 47 HIV patients [mean age 51 years; 81% with HIV RNA ≤. 50 copies/mL] on combination antiretroviral therapy. The gene-specific DNA damage and repair assay measured mtDNA 8-oxo-dG break frequency. Magnetic resonance imaging was performed at 3 T. Higher mtDNA 8-oxo-dG was associated with lateral ventricular enlargement and with decreased volumes of hippocampus, pallidum, and total subcortical gray matter, suggesting the involvement of systemic mitochondrial-specific oxidative stress in chronic HIV-related structural brain changes and cognitive difficulties. Clarification of the mechanism may provide potential therapeutic targets. © 2016 Elsevier B.V. and Mitochondria Research Society.
Shiramizu B.,University of Hawaii at Manoa |
Shiramizu B.,Hawaii Center for AIDS |
Ananworanich J.,University of Hawaii at Manoa |
Ananworanich J.,Red Cross |
And 12 more authors.
Journal of NeuroVirology | Year: 2012
HIV-associated neurocognitive disorders (HAND) persist despite plasma HIV RNA suppression with antiretrovirals (ARV). Sequestered reservoirs in the central nervous system and circulating monocytes are theorized to contribute to persistent brain injury. We previously demonstrated that elevated intracellular HIV DNA from circulating cells was associated with HAND in ARV-treated and ARV-naive subjects. We now report that failure to suppress intra-monocyte HIV DNA 3.5 years after initiating ARV is linked to persistent HAND and subjects with dementia are least likely to suppress intra-monocyte HIV DNA at 3.5 years. These findings suggest that antiviral strategies may need to target intra-monocyte HIV DNA. © 2011 Journal of NeuroVirology, Inc.