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Zheng L.,Central Laboratory | Zheng L.,University of Sichuan | Tan W.,Central Laboratory | Tan W.,Shanghai JiaoTong University | And 4 more authors.
Cancer Immunology, Immunotherapy | Year: 2014

The anti-ErbB2 antibody trastuzumab has currently been approved for ErbB2-positive gastric cancer. Despite the effectiveness of trastuzumab, resistance is common. Thus, there is an urgent need to overcome trastuzumab resistance. Here, we obtain a trastuzumab-resistant cell line, which is derived from the human gastric cancer NCI-N87 cell line, by modeling the development of acquired resistance in patients. Our data show that combining trastuzumab and cetuximab leads to a significant decrease in EGFR/ErbB2 heterodimers and signaling compared with either antibody alone, and the combination results in greater antitumor activity against the trastuzumab-resistant NCI-N87 cell line, both in vitro and in vivo, suggesting that a combined EGFR/ErbB2 inhibition may overcome trastuzumab resistance. © 2014 Springer-Verlag Berlin Heidelberg.

Li P.,Harrison International Peace Hospital
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2013

To investigate the clinical significance of applicating posterior internal fixation for regulating spinal curvature in thoracolumbar compression fractures. Between May 2006 and May 2009, 63 patients with thoracolumbar compression fractures were treated, and the clinical data were retrospectively analyzed. Among them, 33 patients received traditional posterior internal fixation in control group; 30 patients underwent posterior internal fixation with spinal curvature correction under C-arm X-ray device in trial group. There was no significant difference in age, gender, cause of injury, injured segment, grade of fracture, and time from injury to operation between 2 groups (P > 0.05). The Cobb angle, height of injured vertebral body, and disc height were measured by X-ray examination; loosening and breakage of internal fixation were observed and compared between 2 groups. The recovery rate was calculated according to pre- and post-operative visual analogue scale (VAS) and Oswestry disability index (ODI) scores for each patient. All cases were followed up 20-45 months (mean, 31 months). The postoperative VAS score, ODI, Cobb angle, height of injured vertebral body, and disc height were improved significantly when compared with preoperative values in 2 groups (P < 0.05). At last follow-up, VAS and ODI scores of trial group were significantly better than those of control group (P < 0.05); loss of Cobb angle was (2.1 +/- 1.7) degrees in trial group and (4.2 +/- 3.2) degrees in control group, showing significant difference (t=1.457, P=0.000); loss of disc height was (1.4 +/- 1.2) mm in trial group and (3.4 +/- 2.3) mm in control group, showing significant difference (t=9.336, P= 0.000); loss of height of injured vertebral body was 1.8% +/- 0.6% in trial group and 5.4% +/- 2.1% in control group, showing significant difference (t=3.435, P=0.000). Broken screw and loosening screw occurred in 1 case of control group, respectively (6.1%), but no broken or loosening screw in trial group, showing significant difference (P=0.000). Application of posterior internal fixation for regulating spinal curvature has a good clinical effectiveness. The postoperative spinal curvature, the height of injured vertebral body, and disc height can be improved significantly and low back pain can be recovered satisfactorily. The modified technique is also effective in reducing broken and loosening incidence of the fixation system.

Yang B.,Harrison International Peace Hospital | Tan Z.,Jilin University | Song Y.,Jilin University
International Journal of Clinical and Experimental Medicine | Year: 2015

Objective: This study aims to identify the target gene of hsa-miR-195 and to research the molecular mechanism of hsa-miR-195 which is through its target genes in the colorectal cancer invasion and metastasis. Methods: We used biological informatics (RNAhybrid and Target Scan analysis database) to predict the target genes of hsa-miR-195. Collected colon cancer tissues from clinical colorectal cancer patients by surgical removal of the carcinoma and control tissues, and researched the expression of Bcl-2 in tissues by immunohistochemical. Next, Real-time PCR was used to research the expression of hsa-miR-195 in Caco-2 and NCM460 cell line. hsa -miR-195 Mimics was transient transfered to Caco-2 cells, western blot was used to analysis the expression changes of Bcl-2. To analysis the possibility that hsa-miR-195 can affect the invasive ability of tumor cells by Bcl-2, we transferred hsa-miR-195 Mimics and Bcl-2 expression plasmid, and used the cell invasion experiment to discusses hsa-miR-195 effect on the ability of tumor cell invasion. Results: the immunohistochemical results showed that, the semi-quantitative parameters for the Bcl-2: control by 0.89 ± 0.51, 6 colon cancer by 31 ± 0.79. The expression of has-miR-195 in Caco-2 is 0.39 ± 1.5 while the value in control is2.01 ± 0.2, **P < 0.01. Conclusion: In colorectal cancer, has-miR-195 can promote cell apoptosis and inhibit the invasion and metastasis by inhibiting the expression of Bcl-2. © 2015, Int J Clin Exp Med. All rights reserved.

Tang S.-Y.,University of South China | Zhong M.-Z.,Central South University | Yuan G.-J.,Chinese Peoples Liberation Army | Hou S.-P.,Harrison International Peace Hospital | And 3 more authors.
Oncology Reports | Year: 2013

We investigated the effect of casticin on apoptosis induced by tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL). We found that casticin potentiated TRAIL-induced apoptosis in human colon cancer cells. Casticin downregulated cell survival proteins including Bcl-xL, Bcl-2, survivin, XIAP and cFLIP, and induced death receptor 5 (DR5), but had no effect on DR4 and decoy receptors (DcR1 or DcR2). Deletion of DR5 by siRNA significantly reduced the apoptosis induced by TRAIL and casticin. In addition, casticin induced reactive oxygen species (ROS) generation in a dose-dependent manner. Collectively, the present study showed that casticin potentiates TRAIL-induced apoptosis through downregulation of cell survival proteins and induction of DR5 mediated by ROS.

Li M.,Harrison International Peace Hospital | Dong F.,Hebei Medical University | Zhang F.,Hebei Medical University
International Journal of Clinical and Experimental Medicine | Year: 2016

Physical exercise could exert neuroprotection in both human and animals. However, the exact mechanism of the neuroprotective effect is still not very clear. This study was to explore the possible signal pathway related to the neuroprotective effect of pre-ischemic exercise for ischemic stroke. Thirty-six rats were randomly divided into three groups (n = 12/group): middle cerebral artery occlusion (MCAO) group, exercise with MCAO group and sham surgery group. After treadmill training for three weeks, the MCA was occluded for 1.5 hours so as to induce ischemic stroke, followed by reperfusion. Forty-eight hours after reperfusion, six rats in each group were estimated for neurological deficits and then decapitated to calculate the infarct volume. The rest rats in each group were sacrificed to detect the expression level of phosphor-MEK1/2, MEK1/2 and PI3K (n = 6). The results in our study demonstrated that pre-ischemic treadmill training reduced brain infarct volume and neurological deficits, also alleviated the over expression of phosphor-MEK1/2 after ischemic stroke. The expression level of PI3K was up-regulated by exercise preconditioning. In summary, pre-ischemic exercise mitigated brain damage in the rat brain after ischemic stroke, involving in the regulation of MEK1/2 and PI3K. © 2016, E-Century Publishing Corporation. All rights reserved.

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