Harbin Pharmaceutical Group R and nter

China

Harbin Pharmaceutical Group R and nter

China
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Sun Z.,Northeast Forestry University | Sun Z.,Harbin Institute of Technology | Yuan S.,Harbin Institute of Technology | Zhao H.,Harbin Pharmaceutical Group R and nter | And 2 more authors.
Journal of Microencapsulation | Year: 2017

1-Deoxynojirimycin sustained-release pellets, which exhibit known release and absorption profiles, are used for the treatment of diabetes mellitus. In this study, a fluidised bed coater was employed to prepare new, drug-loaded pellets. In the dissolution test, it was found that 1-DNJ pellets exhibited a sustained release effect after being coated with hydroxypropyl methyl cellulose phthalate-55 S. For sustained-release pellets and immediate-release pellets, there was significant difference in the mean cumulative drug concentration profile in different media evaluation. In the bioavailability study, the ratio of mean relative bioavailability of the SR pellets to the IR tablets was calculated by the DAS from the AUC0-24 h of 1-DNJ and was found to be 117.3%. This suggested that the behaviour in vivo of the 1-DNJ SR pellets was superior to the IR tablets, which indicated the designed preparation method of the 1-DNJ SR pellets was acceptable for achieving sustained release of 1-DNJ with enhanced bioavailability. © 2017 Informa UK Limited, trading as Taylor & Francis Group


Jia Q.,R and nter | Wu H.,R and nter | Zhou X.,R and nter | Gao J.,R and nter | And 39 more authors.
Science China Life Sciences | Year: 2010

High mammalian gene expression was obtained for more than twenty different proteins in different cell types by just a few laboratory scale stable gene transfections for each protein. The stable expression vectors were constructed by inserting a naturally-occurring 1.006 kb or a synthetic 0.733 kb DNA fragment (including intron) of extremely GC-rich at the 5′ or/and 3′ flanking regions of these protein genes or their gene promoters. This experiment is the first experimental evidence showing that a non-coding extremely GC-rich DNA fragment is a super "chromatin opening element" and plays an important role in mammalian gene expression. This experiment has further indicated that chromatin-based regulation of mammalian gene expression is at least partially embedded in DNA primary structure, namely DNA GC-content. © Science China Press and Springer-Verlag Berlin Heidelberg 2010.

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