Harbin Pharmaceutical Group Co.

Harbin, China

Harbin Pharmaceutical Group Co.

Harbin, China
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Liu J.,China Pharmaceutical University | Liu J.,Harbin Pharmaceutical Group Co Ltd | Zhu Z.,China Pharmaceutical University | Tang J.,China Pharmaceutical University | And 3 more authors.
Anti-Cancer Agents in Medicinal Chemistry | Year: 2017

A total of 16 targeted NO-releasing betulinic acid (BA) derivatives were designed and synthesized as potential anticancer agents. Most IC50 values were under 1.0 μM in vitro test against HepG2 and B16. The result suggested that derivatives of BA with α,β-unsaturated ketone skeleton possessed significant cytotoxic activities than the others, among which derivatives with three carbons in diol linker (15b and 15c) exhibited the highest anti-cancer activity. NO-releasing amount detection of partial target compounds suggested that NO-releasing amount of this series of BA derivatives positively correlates with their cytotoxic activities. The anti-angiogenic activity of partial target compounds on zebrafish embryos in our experiment did not show any effects on the SIVs, however, they exhibited different influence on ISVs, with only 15a and 15d better than the negative control. © 2017 Bentham Science Publishers.

Li W.-C.,Harbin Pharmaceutical Group Co. | Sun Y.-H.,Heilongjiang University of Chinese Medicine | Xie L.-W.,Harbin Pharmaceutical Group Co. | Fu R.,Harbin Pharmaceutical Group Co. | And 3 more authors.
Chinese Journal of New Drugs | Year: 2014

Objective: To establish an HPLC method for simultaneous determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, forsythoside A, isochlorogenic acid A, rutin, luteoloside, isochlorogenic acid C, forsythin, baicalin, oroxyloside, wogonoside, baicalein and wogonin in Shuanghuanglian powder for injection.Methods: HPLC assay was carried out using a CosmosilⓇ C18-MS-Ⅱcolumn (250 mm×4.6 mm, 5 μm). The mobile phase was methanol-0.2% phosphoric acid solution for gradient elution. The flow rate was 1.0 mL·min-1, and the detection wavelength was set as multi-wavelength detection.Results: The linear ranges of the fifteen components were 0.025~0.153, 0.116~0.696, 0.026~0.156, 0.003~0.020, 0.158~0.946, 0.011~0.067, 0.002~0.012, 0.009~0.056, 0.033~0.195, 0.022~0.134, 1.48~8.87, 0.069~0.412, 0.009~0.054, 0.006~0.035 and 0.002~0.012 μg, respectively. The fifteen components showed a good linear correlation (r≥0.999 5). The average recoveries of fifteen components were in accordance with the determination requirements.Conclusion: The method is simple, quick, reliable and accurate. It is very suitable to control the quality of Shuanghuanglian powder for injection. ©, 2014, Chinese Journal of New Drugs Co. Ltd. All right reserved.

Huang X.-X.,Shenyang Pharmaceutical University | Huang X.-X.,Peking Union Medical College | Guo D.-D.,Shenyang Pharmaceutical University | Guo D.-D.,Peking Union Medical College | And 10 more authors.
Biochemical Systematics and Ecology | Year: 2013

Detailed chemical investigation of the secondary metabolites from the extract of the leaves of Crataegus pinnatifida led to the isolation of 12 monoterpene and sesquilignan compounds, including a new monoterpene, pinnatifidanoid A (1), a new sesquilignan, pinnatifidanin A (12). To our knowledge, this is the first report of the presence of compounds 1-12 in this genus. © 2012 Elsevier Ltd.

Huang X.-X.,Shenyang Pharmaceutical University | Zhou C.-C.,Shenyang Pharmaceutical University | Li L.-Z.,Shenyang Pharmaceutical University | Li F.-F.,Shenyang Pharmaceutical University | And 6 more authors.
Bioorganic and Medicinal Chemistry Letters | Year: 2013

Nine new 8-O-4′ neolignans, named pinnatifidanin B I-IX (1-9), together with 9 known analogs (10-18) were isolated from the seeds of Crataegus pinnatifida. The structures of 1-18 were determined by spectroscopic methods, including 1D, 2D NMR, CD and HRESIMS analysis. Compounds 8-11, 17 and 18 displayed potent cytotoxic activities against human cancer cell lines, and most interestingly, none of the 6 compounds displayed inhibitory activity against human lung cell line (Mrc5). The 6 cytotoxic compounds are considered to be potential as antitumor agents, which could significantly inhibit the cancer cell growth in a dose-dependent manner and are probably safer than positive control drug. © 2013 Elsevier Ltd. All rights reserved.

Meng X.,CAS Changchun Institute of Applied Chemistry | Meng X.,Harbin Pharmaceutical Group CO. | Yang S.,Aerospace 731 Hospital | Pi Z.,CAS Changchun Institute of Applied Chemistry | And 3 more authors.
Journal of Liquid Chromatography and Related Technologies | Year: 2012

The aim of the present research is to find the influence of honey in the metabolism of liquiritin by human intestinal flora to validate the folkloric use of honey-treated licorice (Glycyrrhiza uralensis). The metabolites of liquiritin were analyzed by HPLC-MS/MS and FT-ICR-MS/MS. The immunological effects were investigated by using an in vitro splenocyte proliferation assay. In the presence of honey, liquiritin was metabolized slowly into the main metabolite (liquiritigenin), and the other metabolite (davidigenin) was only found in low abundance. But, in the absence of honey, liquiritin was rapidly transformed into liquiritigenin, and liquiritigenin was further metabolized into davidigenin, making davidigenin the main metabolite. The splenocyte proliferation assay revealed that liquiritigenin shows remarkable immuno-enhancing effects, whereas liquiritin and davidigenin exhibit little or adverse effect on the proliferation of ConA-induced murine splenocytes. The presence of honey can alter the metabolism of liquiritin and, thus, influence the pharmacological effects of the licorice, rendering the honey-treated licorice as a medicine to invigorate the spleen. © 2012 Copyright Taylor and Francis Group, LLC.

Zhou C.-C.,Shenyang Pharmaceutical University | Huang X.-X.,Shenyang Pharmaceutical University | Gao P.-Y.,Shenyang University of Chemical Technology | Li F.-F.,Shenyang Pharmaceutical University | And 3 more authors.
Journal of Asian Natural Products Research | Year: 2014

One new sesquiterpene, (1,4aβ,8a)-1-isopropanol-4a-methyl-8- methylenedecahydronaphthalene (1), with one new phenylpropanoid, threo-2-(4-hydroxy-3,5-dimethoxyphenyl)-3-(4-hydroxy-3-methoxyphenyl) -3-ethoxypropan-1-ol (2), along with four known phenylpropanoids were isolated from Crataegus pinnatifida. The structures of compounds 1 and 2 were elucidated on the basis of 1D, 2D NMR analyses, and HR-ESI-MS. The antithrombotic activity in vitro of all isolates was assayed, and only compound 1 exhibited potent antithrombotic activity by inhibiting platelet aggregation in rat plasma by 81.4% at 1 mg/ml. © 2013 Taylor & Francis.

Tang J.-Y.,Shenyang Pharmaceutical University | Wang W.,Shenyang Pharmaceutical University | Li L.,Harbin Pharmaceutical Group Co. | Zhang C.-G.,Shenyang Pharmaceutical University | And 2 more authors.
Yaoxue Xuebao | Year: 2012

Ranolazine and metabolites in dog urine were identified by LC-MS n. Dog urine samples were collected after ig 30 mg·kg -1 ranolazine, then the samples were enriched and purified through solid-phase extraction cartridge. The purified samples were analyzed by LC-MSn. The possible metabolites were discovered by comparing the full scan and SIM chromatograms of the test samples with the corresponding blanks. Seventeen phase I metabolites and fourteen phase II metabolites were identified in dog urine. Three metabolites were identified by comparing with the control article. The metabolites were formed via the following metabolic pathways: O-demethylation, O-dearylation, hydroxylation, N-dealkylation, amide hydrolysis, glucuronidation and sulfation. The LC-MSn method is suitable for the rapid identification of drug and its metabolites in biologic samples.

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