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Sarker K.K.,China Agricultural University | Wang X.,CAU | Li H.,CAU | Xu C.,Harbin Academy of Agricultural science | And 2 more authors.
AMA, Agricultural Mechanization in Asia, Africa and Latin America | Year: 2015

Tillage, fertilizer placement and water are the most important for sustainable and economical agriculture. Therefore, a zone-till subsurface row fertilizer applicator was developed and evaluated at Harbin, China. The system of the applicator (T1) was compared with traditional rice practices (T2). The performance tests of the applicator were promising. The system (T1) saved water, reduces costs and desired transplanted seedling depth compared to T2. The new system can enrich soil temperature, crop growth rate, yields and increased the residual retention of nutrients (N, P, K) in soil. The applicator and the system could be offered as an alternative for future pattern of rice cultivation.


Xu G.,Northeast Agricultural University | Huang T.,Northeast Agricultural University | Jin X.,Harbin Academy of Agricultural Science | Cui C.,Harbin Academy of Agricultural Science | And 4 more authors.
Fish Physiology and Biochemistry | Year: 2015

In non-mammalian vertebrates, estrogens and expressions of cyp19a1 and foxl2 play critical roles in maintaining ovary differentiation and development, while dmrt1 and sox9 are male-specific genes in testicular differentiation and are highly conserved. In order to deeply understand the morphological change, sex steroids level and molecular mechanism of triploid female gonadal reversal in rainbow trout, we studied the ovary morphology, tendency of estradiol-17β (E2) and testosterone (T) levels and the relative expressions of dmrt1, cyp19a1, sox9 and foxl2 in juvenile and adult fish. Our results demonstrated that the development of triploid female gonads in rainbow trout went through arrested development, oocytes dedifferentiation, ovary reconstruction and sex reversal finally. During early gonadal development (154–334 days post-fertilization), the expressions of foxl2 and cyp19a1 increased linearly, while expressions of dmrt1 and sox9 were extremely suppressed, and E2 level was higher, while T level was lower. During the mid-to-late period of triploid female gonadal development (574–964 days post-fertilization), the expressions of dmrt1 and sox9 remained high and were very close to the quantity of diploid male genes, and T levels were even reaching diploid male plasma concentrations, while expressions of cyp19a1 and foxl2 were decreased, leading to decrease in E2 level. We realized that the development model of rainbow trout triploid female gonads was extremely rare, and the regulatory mechanism was very special. Genes involved in gonadal development and endogenous estrogens are pivotal factors in fish natural sex reversal. © 2015 Springer Science+Business Media Dordrecht


PubMed | Chinese Academy of Fishery Sciences, Northeast Agricultural University and Harbin Academy of Agricultural Science
Type: Journal Article | Journal: Fish physiology and biochemistry | Year: 2016

In non-mammalian vertebrates, estrogens and expressions of cyp19a1 and foxl2 play critical roles in maintaining ovary differentiation and development, while dmrt1 and sox9 are male-specific genes in testicular differentiation and are highly conserved. In order to deeply understand the morphological change, sex steroids level and molecular mechanism of triploid female gonadal reversal in rainbow trout, we studied the ovary morphology, tendency of estradiol-17 (E2) and testosterone (T) levels and the relative expressions of dmrt1, cyp19a1, sox9 and foxl2 in juvenile and adult fish. Our results demonstrated that the development of triploid female gonads in rainbow trout went through arrested development, oocytes dedifferentiation, ovary reconstruction and sex reversal finally. During early gonadal development (154-334 days post-fertilization), the expressions of foxl2 and cyp19a1 increased linearly, while expressions of dmrt1 and sox9 were extremely suppressed, and E2 level was higher, while T level was lower. During the mid-to-late period of triploid female gonadal development (574-964 days post-fertilization), the expressions of dmrt1 and sox9 remained high and were very close to the quantity of diploid male genes, and T levels were even reaching diploid male plasma concentrations, while expressions of cyp19a1 and foxl2 were decreased, leading to decrease in E2 level. We realized that the development model of rainbow trout triploid female gonads was extremely rare, and the regulatory mechanism was very special. Genes involved in gonadal development and endogenous estrogens are pivotal factors in fish natural sex reversal.


Huo T.B.,Heilongjiang River Fishery Research Institute | Yuan M.Y.,Harbin Academy of Agricultural science | Jiang Z.F.,Heilongjiang River Fishery Research Institute
Journal of Applied Ichthyology | Year: 2011

In this study the length-weight relationships of 23 fish species belonging to eight families and 21genera from the Ergis River in China were analyzed. A total of 1869 specimens were used to estimate the relationship parameters. Seven of these fish species and one maximum length are new to science against FishBase. The r2 value ranged from 0.975 to 0.999. Values of b varied from 2.630 to 3.494. © 2010 Blackwell Verlag, Berlin.


Li L.,Chinese Academy of Agricultural Sciences | Li L.,Harbin Academy of Agricultural science | Wang W.,Chinese Academy of Agricultural Sciences | Huang S.,Chinese Academy of Agricultural Sciences | And 3 more authors.
Australian Journal of Crop Science | Year: 2014

A full-length mRNA that encoded a plasma membrane intrinsic protein (PIP) was isolated by electronic elongation and RT-PCR, containing an open reading frame of 861bp (GenBank HM245628), which encodes a protein of 281 amino acid residues whit a termination codon. The GmPIP included an Asn-Pro-Ala (NPA) domain and major intrinsic protein (MIP) superfamily domain, protein sequence alignment studies and phylogenetic analysis strongly suggested that GmPIP belongs to the group of plant PIPs. The semi-quantitative RT-PCR results showed that GmPIP mRNA abundance was increased in roots under treatments with salt. Sense and antisense expression vectors of GmPIP gene were constructed. Transgenic soybean hair roots overexpressing sense GmPIP gene showed stronger viability than the hair roots overexpressing antisense GmPIP gene and control upon salt stress. The transgenic Lotus corniculatus line was transferred to MS medium (pH6.8) containing 150 mM NaCl. About 15 days later, the transgenic L. Corniculatus plants had better growth than the wild plants. Therefore GmPIP may be involved into salt tolerance, and the enhanced expression of GmPIP gene may have caused the increased salt tolerance of soybean.


Jiang X.,Northeast Agricultural University | Jiang X.,National and Local Joint Freshwater Fish Breeding Engineering Laboratory of China | Guan X.,Northeast Agricultural University | Yao L.,Northeast Agricultural University | And 4 more authors.
Biological Trace Element Research | Year: 2015

Copper (Cu) and cadmium (Cd) are the most common heavy metals that are easily detected in aquatic environments on a global scale. In this paper, we investigated the messenger RNA (mRNA) and protein levels of HSPs (HSP60, HSP70, and HSP90) in the liver of the common carp exposed to Cu, Cd, and a combination of both metals by real-time quantitative PCR and Western blot. The results indicated that in each exposure group, the mRNA levels of HSP60, HSP70, and HSP90 were increased significantly compared to the corresponding controls after 96 h of exposure (P < 0.05). A significant increase was observed in the HSP70 protein level in the high-dose Cu group and all of the Cd groups. Significant increases were also observed in the protein levels of HSP60 and HSP90 in the high combination group and the low combination group, respectively. These results indicated that the dynamics of HSP expression observed in the common carp support the role of HSPs as biochemical markers in response to environmental pollution and provided valuable insights into the adaptive mechanisms used by the common carp to adapt to the challenges of stressful environments. © 2015 Springer Science+Business Media New York


Wang J.,Northeast Agricultural University | Zhang C.,Northeast Agricultural University | Xu C.,Harbin Academy of Agricultural science | Kong Y.,Northeast Agricultural University | Zhao J.,Northeast Agricultural University
Nongye Jixie Xuebao/Transactions of the Chinese Society for Agricultural Machinery | Year: 2013

In order to determine the key parameters of the flattening component on combined cultivating implement, the field experiment was conducted by using the flattening device. The three factors and three levels orthogonal regressive tests were designed in the experiment with working velocity, diameter of the field-ditch filling disc, angle of the field-ditch filling disc as influence factors and the soil-breaking rate as response index. The regression mathematics models between response index and influence factors were established and the parameters were optimized through Design-Expert. The optimal combination was that working velocity of 10.63 km/h, diameter of the field-ditch filling disc of 450 mm, angle of the field-ditch filling disc of 17.71°. At that time the soil-breaking rate was 91.92%, which could meet the needs of agricultural demands.


PubMed | Northeast Agricultural University and Harbin Academy of Agricultural science
Type: Journal Article | Journal: Biological trace element research | Year: 2016

Copper (Cu) and cadmium (Cd) are the most common heavy metals that are easily detected in aquatic environments on a global scale. In this paper, we investigated the messenger RNA (mRNA) and protein levels of HSPs (HSP60, HSP70, and HSP90) in the liver of the common carp exposed to Cu, Cd, and a combination of both metals by real-time quantitative PCR and Western blot. The results indicated that in each exposure group, the mRNA levels of HSP60, HSP70, and HSP90 were increased significantly compared to the corresponding controls after 96h of exposure (P<0.05). A significant increase was observed in the HSP70 protein level in the high-dose Cu group and all of the Cd groups. Significant increases were also observed in the protein levels of HSP60 and HSP90 in the high combination group and the low combination group, respectively. These results indicated that the dynamics of HSP expression observed in the common carp support the role of HSPs as biochemical markers in response to environmental pollution and provided valuable insights into the adaptive mechanisms used by the common carp to adapt to the challenges of stressful environments.

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