Cai L.-F.,Zhejiang University |
Cai L.-F.,Hanshan Normal University |
Zhu Y.,Zhejiang University |
Du G.-S.,Zhejiang University |
Fang Q.,Zhejiang University
Analytical Chemistry | Year: 2012
We described a microfluidic chip-based system capable of generating droplet array with a large scale concentration gradient by coupling flow injection gradient technique with droplet-based microfluidics. Multiple modules including sample injection, sample dispersion, gradient generation, droplet formation, mixing of sample and reagents, and online reaction within the droplets were integrated into the microchip. In the system, nanoliter-scale sample solution was automatically injected into the chip under valveless flow injection analysis mode. The sample zone was first dispersed in the microchannel to form a concentration gradient along the axial direction of the microchannel and then segmented into a linear array of droplets by immiscible oil phase. With the segmentation and protection of the oil phase, the concentration gradient profile of the sample was preserved in the droplet array with high fidelity. With a single injection of 16 nL of sample solution, an array of droplets with concentration gradient spanning 3-4 orders of magnitude could be generated. The present system was applied in the enzyme inhibition assay of β-galactosidase to preliminarily demonstrate its potential in high throughput drug screening. With a single injection of 16 nL of inhibitor solution, more than 240 in-droplet enzyme inhibition reactions with different inhibitor concentrations could be performed with an analysis time of 2.5 min. Compared with multiwell plate-based screening systems, the inhibitor consumption was reduced 1000-fold. © 2011 American Chemical Society.
Li J.,Hanshan Normal University
Optics Express | Year: 2014
In this paper, a novel secure optimal image watermarking scheme using an encrypted gyrator transform computer generated hologram (CGH) in the contourlet domain is presented. A new encrypted CGH technique, which is based on the gyrator transform, the random phase mask, the three-step phase-shifting interferometry and the Fibonacci transform, has been proposed to produce a hologram of a watermark first. With the huge key space of the encrypted CGH, the security strength of the watermarking system is enhanced. To achieve better imperceptibility, an improved quantization embedding algorithm is proposed to embed the encrypted CGH into the low frequency sub-band of the contourlettransformed host image. In order to obtain the highest possible robustness without losing the imperceptibility, particle swarm optimization algorithm is employed to search the optimal embedding parameter of the watermarking system. In comparison with other method, the proposed watermarking scheme offers better performances for both imperceptibility and robustness. Experimental results demonstrate that the proposed image watermarking is not only secure and invisible, but also robust against a variety of attacks. © 2014 Optical Society of America.
Li L.,Hanshan Normal University
Physical Review A - Atomic, Molecular, and Optical Physics | Year: 2012
In this paper, we consider two related problems, unambiguous discrimination of quantum mixed states and unambiguously conveying classical messages using Einstein-Podolsky-Rosen (EPR) pairs. The main technical contributions are as follows. First, we present a general upper bound on the success probability for unambiguous discrimination among arbitrary N mixed quantum states with given a priori probability, which can be directly used to the unambiguous communication setting. Second, we propose a sufficient condition to show that a new upper bound can be achievable, and construct examples to clarify the relationship between the upper bound we derive and the existing upper bounds presented by other authors. Third, in the issue of conveying classical messages using EPR pairs, we also derive an upper bound on the success probability for unambiguously decoding, where Alice wants to convey n bit classical information to Bob by sending m quantum bits. © 2012 American Physical Society.
Li J.,Hanshan Normal University
Applied Optics | Year: 2010
Robustness against geometric attacks is one of the most important issues in digital watermarking. A novel geometric robust watermarking scheme that uses computer-generated holograms as the watermark is presented. To maintain imperceptibility and robustness, a quantization embedding algorithm is adopted to embed the mark hologram into the low-frequency subband of the wavelet-transformed host image. In the detection process, the geometric distorted watermarked images are recovered first by the proposed improved geometric correction method, which is based on the scale invariant feature transform, the invariant centroid, and the pulse coupled neural network. Then the mark holograms are extracted from the recovered images. In comparison with the traditional geometric estimation method, the suggested improved geometric correction method can estimate the geometric distortion parameters more accurately and needs less auxiliary information. Compared with other watermark schemes using digital holograms, the proposed method has the distinct advantage of robustness to geometric attacks. The experimental results demonstrate that the proposed method has good robustness to resist geometric attacks and common attacks including rotation, scaling, translation, image flipping, combined attacks, filtering, occlusion, cropping, and JPEG compression. © 2010 Optical Society of America.
Huang M.,Hanshan Normal University
Computers and Mathematics with Applications | Year: 2010
By using an impulsive inequality and the Riccati transformation technique on time scales, several oscillation criteria are established for the second order nonlinear dynamic equations on time scales with impulses. Examples are given to show that the impulses play a dominant part in the oscillations of dynamic equations on time scales. © 2009 Elsevier Ltd. All rights reserved.