Dresden, Germany
Dresden, Germany

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Olbricht K.,Hansabred GmbH and Co. KG | Ludwig A.,Hansabred GmbH and Co. KG | Ulrich D.,Julius Kuhn Institute | Spangenberg R.,TU Dresden | And 2 more authors.
Acta Horticulturae | Year: 2014

For the first time, the genus of Fragaria was investigated by electron microscopy with regard to epicuticular wax structures and trichome formation on adaxial and abaxial leaf surfaces. In addition, cross-sections and volatile patterns of leaves of several genotypes were analyzed. Distinct differences of epicuticular wax structures among the species were observed on the adaxial but not on the abaxial leaf side. Obviously, epicuticular wax structures are not essential for susceptibility reactions to fungal diseases. Epidermal wall thickness as a reason for resistance to leaf diseases can only be supported for individual examples. Resistance to spider mite (Tetranychus urticae Koch) can be traced back to trichome formation on the abaxial side of the leaf. Additionally, the volatile analysis revealed the release of VOCs as a response to the spider mite attack.


Olbricht K.,Hansabred GmbH and Co. KG | Ulrich D.,Julius Kuhn Institute | Weiss K.,Julius Kuhn Institute | Grafe C.,Julius Kuhn Institute
Journal of Agricultural and Food Chemistry | Year: 2011

Volatile metabolites are a basis for sensory and resistance traits of Fragaria × ananassa. Stability of expression is important for the selection of cultivars. For the first time, the stability of volatiles in a strawberry population after cross-combination of two distinct cultivars ('Mieze Schindler' × 'Elsanta') has been investigated. In this work, environmentally caused variations in the synthesis of 18 volatiles were studied over two years using a model population of 158 clones. The stability varied throughout the F1 seedling population between the two years, defining stable and unstable genotypes with respect to volatile synthesis. Most of the stable genotypes exhibited low values in relative volatile concentration. Merely 6 stable volatiles were detected in the parental cultivars, whereas about 40% of the F1 progeny had up to 11 stable volatiles. Consequently, a higher stability in volatile synthesis can be achieved by breeding. © 2011 American Chemical Society.


Ulrich D.,Julius Kuhn Institute | Olbricht K.,Hansabred GmbH and Co. KG | Thomas E.,Goethestr. 23
Acta Horticulturae | Year: 2014

Flavour is one of the key attributes of strawberry fruit quality. Out of the more than 300 volatile organic compounds (VOCs) identified so far around 20-30 character impact compounds dominate the typical aroma impression. Since the aroma composition is influenced by several parameters like cultivar, maturity, climate and cultivation system adequate methods for berry sampling, sample preparation, separation and data processing are required to determine the dynamic and diversity of aroma composition. The diversity of VOC patterns for 15 F. vesca accessions is discussed in comparison to F. xananassa. © 2014, International Society for Horticultural Science. All rights reserved.


Ulrich D.,Julius Kuhn Institute | Ulbricht K.,Hansabred GmbH and Co. KG
Journal of Applied Botany and Food Quality | Year: 2013

Fragaria vesca is the most distributed wild species in the genus Fragaria. Due to this biogeography, a high diversity is to expect. During two harvest seasons, sixteen accessions from different locations from the most eastern habitat at Lake Baikal in Siberia, from Middle and Southern Europe and Northern Europe with Scandinavia and Iceland were investigated as well as two of the three described North American subspecies and three F. vesca cultivars. Five very distinct European F. xananassa cultivars were chosen to serve as a comparison. Beside brix value and acid contents, the aroma patterns including 67 volatile compounds were quantified by stir bar sorptive extraction followed by gas chromatography with mass spectrometric detection. The diversity of important volatiles of Fragaria vesca and F. xananassa is discussed regarding biogeography, domestication, breeding and the so-called funnel effect.


Ulrich D.,Julius Kuhn Institute | Olbricht K.,Hansabred GmbH and Co. KG
Journal of Berry Research | Year: 2014

In breeding programs wild strawberry species are used increasingly to enhance the genetic diversity and to implement higher sensory quality. Next to Fragaria moschata L. and F. viridis Weston wild collections cultivars of Fragaria vesca L. were the standards for strawberry taste in Europe before the development of Fragaria × ananassa Duch. around 250 years ago. Therefore, the objective of this research was the evaluation of patterns of volatile organic compounds including around twenty strawberry character impact compounds evaluated for Fragaria vesca and in comparison for standard cultivars of Fragaria × ananassa. The metabolic patterns were measured using immersion-SBSE-GC-MS. Sixteen accessions of Fragaria vesca exhibit a high diversity in qualities and quantities of volatile organic compounds (VOCs). A specific fruit sampling strategy equalizes environmental caused variation and avoids misleading interpretations. The shown results are valuable for further breeding activities regarding flavor, transcriptomic analyses, studies of biochemical pathways and signaling compounds as well as marker development. © 2014 - IOS Press and the authors. All rights reserved.


Hofer M.,Julius Kuhn Institute | Drewes-Alwarez R.,FH Dresden | Scheewe P.,FH Dresden | Olbricht K.,Hansabred GmbH and Co. KG
Journal of Berry Research | Year: 2012

Strawberry cultivars in current commercial use have a very narrow genetic base and reduced morphological and genetic diversity that puts them at risk from disease and climate change. Germplasm collections hold examples of the agricultural biodiversity of small berries where they are conserved, characterized and made available to breeders to improve productivity. Phenological evaluations of existing strawberry cultivars provide information for use by breeders in developing cultivars with improved qualities and more diverse genetic background. Leaves, flowers and fruits are the most important morphological descriptors for identification of strawberry cultivars. A comprehensive catalogue of descriptors drawn up by UPOV and IPGRI served as the basis for evaluation of 108 cultivars in a field trial. Each plot consisted of 21 one-year-old plants per genotype and plants were evaluated once with a range of descriptors; 10 of plant and leaf morphology, 7 of the flower and 25 of the fruit. On the basis of this comprehensive evaluation and data analysis, eighteen primary descriptors (plant, leaf, flower and fruit characteristics) independent of cultivation effects were selected for a large screening. Further descriptors for yield, inner fruit quality and disease resistance should be used as secondary descriptors as they are more environmentally influenced. These descriptors were proposed as appropriate primary and secondary descriptors for the European GENBERRY project of the cultivated strawberry (Fragaria x ananassa Duch.). © 2012-IOS Press and the authors. All rights reserved.


Ludwig A.,Hansabred GmbH and Co. KG | Olbricht K.,Hansabred GmbH and Co. KG | Bartho M.,FH Dresden | Scheewe P.,FH Dresden | Boehme M.,Humboldt University of Berlin
Acta Horticulturae | Year: 2013

Fragaria virginiana Miller and Fragaria chiloensis (L.) Miller are the progenitors of Fragaria × ananassa Duch. Nowadays, innovative strawberry breeding programmes focus on back-crossing strawberry cultivars with their wild relatives. The introgression of accessions of wild species is valuable for cultivar improvement because funnel effects of aroma patterns and resistances are evident in the breeding history of Fragaria × ananassa. In this contribution, Fragaria virginiana seeds from five Canadian sources were sown. The resulting plants were cultivated and taxonomically described as F. virginiana ssp. platypetala, F. virginiana ssp. virginiana and the nothomorph form F. virginiana ssp. glauca × F. virginiana ssp. platypetala. The plants were described regarding growth habit, resistances, flower characteristics including sex, earliness of fruit ripening, fruit shape, size, colour, flavour, yield, Brix value and day neutrality. The selection of individuals resulted in a group of potential breeding partners with promising parameters, in particular, everbearing behaviour. These plants have been added to the Hansabred germplasm collection and they are already being used for breeding new cultivars.


Olbricht K.,Hansabred GmbH and Co. KG | Gerischer U.,Hansabred GmbH and Co. KG | Ludwig A.,Hansabred GmbH and Co. KG | Kraege S.,Hansabred GmbH and Co. KG | And 6 more authors.
Acta Horticulturae | Year: 2014

In 2008, Hansabred GmbH & Co. KG was founded as a European strawberry breeding and research company and has four widely known European companies as shareholders: Kraege Beerenpflanzen (Germany), Vissers (The Netherlands), R.W. Walpole Strawberry Plants Ltd. (UK) and Plantas de Navarra, S.A. (Spain). 250 years of strawberry breeding have led to numerous so-called funnel effects which resulted in susceptibilities to pathogens and in losses of flavour characterised by aroma patterns with low or even zero key volatile concentrations. In this contribution, important aspects of the Hansabred breeding programme are portrayed: high resistance to different pathogens and improved strawberry flavour. A large germplasm collection which originated from the "Professor Staudt Collection" is available with more than 260 accessions of wild species and more than 100 cultivars. This background allows innovative pathways in strawberry breeding. Today, nine vigorous clones with excellent flavour are in European trials already. Hansabred has initiated a project network together with fruit processing companies as well as national and international research institutes and universities.


Pinker I.,Humboldt University of Berlin | Olbricht K.,Humboldt University of Berlin | Olbricht K.,Hansabred GmbH and Co. KG | Pohlheim F.,Brodberg 38
Acta Horticulturae | Year: 2012

Tunnel effects in 250 years of strawberry breeding history resulted in high performance cultivars with reduction in quality traits connected with flavour and resistances. Genetic resources for important traits are found in wild species such as Fragaria vesca. Mutation breeding of F. vesca up to an octoploid chromosome set is necessary for cross-breeding on a balanced ploidy level with Fragaria ×ananassa Duch. Here, we report on the treatment of axillary seedling buds with amiprofosmethyl (APM) which resulted in sectorial, mericlinal and periclinal ploidy chimeras. Chimeral segregation was performed via callus culture as well as using the generative way (selfing). Callus development and shoot regeneration on selected leaf segments depended on the composition of the medium and varied among the repetitions. The regeneration of adventitious shoots started after six to eight weeks on the induction medium and lasted four to six months. Evaluation of ploidy level was done by e.g., phenotype evaluation, comparison of leaf morphology, stomata measurement, flow cytometry and chromosome counting. From the mutagen treatment to the first fruits of the homohistic regenerates, a period of 18 months was sufficient via callus culture. For generative segregation a time span of 30 months was needed. More tetraploid plants were gained from callus culture than from generative segregation. The auto-tetraploid mutants do not differ in fertility and were used in test crosses with F. ×ananassa resulting in hexaploid hybrids. A second mutagen treatment is being executed to obtain auto-octoploids via polyploidisation of the tetraploid F. vesca.

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