Bae J.E.,Hannam University |
Kim J.,Hans Biomedical Corporation |
Ahn J.,Hans Biomedical Corporation |
Choi D.M.,Hans Biomedical Corporation |
And 3 more authors.
Korean Journal of Microbiology and Biotechnology | Year: 2010
Acellular dermal matrix (ADM), produced by decellularization from human cadaveric skin, has been used for various biomedical applications. A manufacturing process for ADM (SureDerm™) using tri-n-butyl phospahate (TnBP) and deoxycholic acids as the decellularization solution has been developed. The manufacturing process for SureDerm™ has 70% ethanol treatment and ethylene oxide gas sterilization for inactivating infectious microorganisms. The purpose of this study was to examine the efficacy of the 70% ethanol treatment, decellularization process using 0.1% TnBP and 2% deoxycholic acids, and EO gas sterilization process in the inactivation of viruses. A variety of experimental model viruses for human pathogens, including the human immunodeficiency virus type 1 (HIV-1), bovine herpes virus (BHV), bovine viral diarrhoea virus (BVDV), hepatitis A virus (HAV), and porcine parvovirus (PPV) were all selected for this study. Enveloped viruses such as HIV-1, BHV, and BVDV were effectively inactivated to undetectable levels by 70% ethanol treatment. However HAV and PPV showed high resistance to 70% ethanol treatment with the log reduction factors of 1.85 and 1.15, respectively. HIV-1, BHV, and BVDV were effectively inactivated to undetectable levels by decellularization process. All the viruses tested were completely inactivated to undetectable levels by EO gas treatment. The cumulative log reduction factors of HIV-1, BHV, BVDV, HAV, and PPV were ≥12.71, ≥18.08, ≥14.92, ≥6.57, and ≥7.18, respectively. These results indicate that the production process for SureDerm™ has a sufficient virus-reducing capacity to achieve a high margin of the virus safety.
Lee J.H.,Korea Institute of Ceramic Engineering And Technology |
Ko I.H.,Korea Institute of Ceramic Engineering And Technology |
Jeon S.-H.,Hans Biomedical Corporation |
Chae J.H.,Hans Biomedical Corporation |
And 2 more authors.
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2011
This study describes the preparation of hydroxyapatite microspheres for local drugs delivery. The formation of the hydroxyapatite microspheres was initiated by enzymatic decomposition of urea and accomplished by emulsification process (water-in-oil). The microspheres obtained were sintered at 500°C. Scanning electron microscope (SEM) indicated that the microspheres have various porous with random size, which maximizes the surface area. Cytotoxicity was not observed after sintering. Osteoporosis drugs, alendronate and BMP-2, were loaded into HAp microspheres and the releases of both molecules showed sustained releasing profiles. © 2011 SPIE.
Koo A.N.,Kyung Hee University |
Kwon I.K.,Kyung Hee University |
Lee S.C.,Kyung Hee University |
Lee S.-K.,Kyung Hee University |
And 5 more authors.
Macromolecular Research | Year: 2010
Novel porous poly(L-lactide) (PLLA) scaffolds with surface-immobilized nano-hydroxyapatite (N-HAp) were synthesized for effective bone regeneration. N-Hap-bearing surface phosphate functionalities were immobilized chemically on the pore surface of amine-treated PLLA scaffolds. Thermogravimetric analysis (TGA) showed that 24 wt% of N-HAp had been immobilized on the scaffold pore surface. Field-emission scanning electron microscopy (FE-SEM) and X-ray photoelectron spectroscopy (XPS) confirmed that N-HAp was exposed markedly on the pore surfaces of scaffolds compared to the conventional scaffold fabricated by a bulk-mixing process of PLLA and N-HAp. The PLLA scaffold with surface-immobilized N-HAp had a higher surface Ca atomic ratio (4.6%) than that on the pore surface of the bulk-mixed PLLA/N-HAp scaffold (Ca = 0.4%). The porous PLLA scaffolds with surface-immobilized N-HAp provided favorable environments for enhanced in vivo bone tissue growth, which was estimated by hematoxylin and eosin (H&E) staining for organic bone tissues and alizarin red S staining for inorganic bone minerals. These results suggest that the N-HAp immobilization approach may produce many useful scaffolds with pore surfaces featuring excellent bone tissue-regenerative properties. [Figure not available: see fulltext.] © 2010 The Polymer Society of Korea and Springer Netherlands.
Hans Biomedical Corporation | Date: 2015-07-28
Bone implants consisting of biological bone tissue for use by orthopedic surgeons, neurosurgeons and oral surgeons.