Qui Nhon, Vietnam

Hanoi University of Agriculture is an education and research university specializing for agricultural sector. The university is located in Trau Quy town, Gia Lam district, a Hanoi suburban area, about 12 km far from Hanoi city centre. Wikipedia.

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Thien Thu C.T.,Hanoi University of Agriculture | Cuong P.H.,National Institute of Animal science | Hang L.T.,National Institute of Animal science | Chao N.V.,Hue University | And 3 more authors.
Journal of Cleaner Production | Year: 2012

This survey was carried out to study animal manure management on livestock farms with biogas technology (biogas farms) and without (non-biogas farms) in the areas surrounding the Vietnamese cities Hanoi and Hue. The objective of the study was to assess the contribution of biogas production to a better environment as well as to recognize the problems with livestock manure management on small-scale farms. On all the farms included in the study more than one manure management technology was used, i.e. composting, separation of manure, biogas production and discharge of liquid manure to recipients such as public sewers or ponds. On biogas farms, most of the manure collected was used for bio-digestion. The farmers used the fermented manure (digestate) as a source of nutrients for crops, but on more than 50% of the interviewed biogas farms digestate was discharged to the environment. On non-biogas farms, manure was in the form of slurry or it was separated into a liquid and a dry-matter-rich solid fraction. The solid fraction from separation was used for composting and the liquid fraction usually discharged to the environment. The survey revealed that there is a need to improve methods for transporting the manure to the field, as transportation is the main barrier to recycling the liquid manure fraction. Farmers in developing countries need financial and technical support to install biogas digesters and to overcome the problems involved in utilizing the manure. Information about how to pre-treat manure before adding it to the digester is urgently needed. At present too much water is used, and the high volume of slurry reduces the retention time and is a disincentive for transporting and applying the digestate to fields. The users need to be informed about the risk of loss of methane to the environment, how to prevent cooker corrosion and the discharge to recipients. In addition, the study reveals that in developing countries manure management legislation needs to be tightened to control environmental pollution. © 2011 Elsevier Ltd. All rights reserved.

Kararigas G.,Charite University Hospital | Nguyen B.T.,Goettingen University Hospital | Nguyen B.T.,Hanoi University of Agriculture | Jarry H.,Goettingen University Hospital
Molecular and Cellular Endocrinology | Year: 2014

The modulation of cardiac growth by estrogen in healthy mice is not completely understood. The aim was to investigate the effects of estrogen on cardiac growth in healthy mice lacking either estrogen receptor (ER) α or β. Wild-type (WT), ERα knockout (ERKO) and ERβ knockout (BERKO) 2-month-old mice were ovariectomized and randomly assigned to groups receiving an estradiol (E2)-containing or soy-free (control, CON) diet ( n= 5-7/group). After three months of E2 administration, WT and BERKO mice had significantly lower body weight, higher relative uterus and heart weight than CON mice, while there was no major E2 effect in ERKO mice. Furthermore, there was a higher concentration of E2-responsive genes Igf1 and Myocd in WT and BERKO but not in ERKO mice. Together, these findings indicate that the estrogenic regulation of cardiac growth in healthy mice is primarily mediated through ERα and not ERβ. © 2013 Elsevier Ireland Ltd.

Nguyen D.T.,University of Science | Le T.H.,University of Science | Bui T.T.T.,Hanoi University of Agriculture
European Journal of Medicinal Chemistry | Year: 2013

Reaction of N-(2,3,4,6-tetra-O-acetyl-β-d-galactopyranosyl) thiosemicarbazide and different substituted benzaldehydes gave some new substituted benzaldehyde N-(2,3,4,6-tetra-O-acetyl-β-d-galactopyranosyl) thiosemicarbazones. The reaction was performed using conventional and microwave-assisted heating methods. The structures of thiosemicarbazones were confirmed by spectroscopic (IR, 1H NMR, 13C NMR and ESI-MS) method. The antioxidant activity of these thiosemicarbazones was evaluated in vitro and in vivo, and it's shown that some of these compounds had significant antioxidant activity. Amongst the compounds screened for antioxidant activity, thiosemicarbazones 4a, 4b and 4c showed good antioxidant activity on DPPH. The compounds 4g, 4i, 4l caused significant elevation of SOD activity and 4e, 4g, 4i, 4l had higher catalase activity, and only compounds 4c and 4f expressed the GSH-Px activity.

Huy G.D.,East China University of Science and Technology | Huy G.D.,Hanoi University of Agriculture | Zhang M.,East China University of Science and Technology | Zuo P.,East China University of Science and Technology | Ye B.-C.,East China University of Science and Technology
Analyst | Year: 2011

A colorimetric assay has been developed for the simultaneous selective detection of silver(i) and mercury(ii) ions utilizing metal nanoparticles (NPs) as sensing element based on their unique surface plasmon resonance properties. In this method, sulfhydryl group modified cytosine-(C)-rich ssDNA (SH-C-ssDNA) was self-assembled on gold nanoparticles (AuNPs) to produce the AuNPs-C-ssDNA complex, and sulfhydryl group modified thymine-(T)-rich ssDNA (SH-T-ssDNA) was self-assembled on silver nanoparticles (AgNPs) to produce the AgNPs-T-ssDNA complex. Oligonucleotides (SH-C-ssDNA or SH-T-ssDNA) could enhance the AuNPs or AgNPs against salt-induced aggregation. However, the presence of silver(i) ions (Ag +) in the complex of ssDNA-AuNPs would reduce the stability of AuNPs due to the formation of Ag + mediated C-Ag +-C base pairs accompanied with the AuNPs color change from red to purple or even to dark blue. Moreover, the presence of mercury(ii) ions (Hg 2+) would also reduce the stability of AgNPs due to the formation of Hg 2+ mediated T-Hg 2+-T base pairs accompanied with the AgNPs color change from yellow to brown, then to dark purple. The presence of both Ag + and Hg 2+ will reduce the stability of both AuNPs and AgNPs and cause the visible color change. As a result, Ag + and Hg 2+ could be detected qualitatively and quantitatively by the naked eye or by UV-vis spectral measurement. The lowest detectable concentration of a 5 nM mixture of Ag + and Hg 2+ in the river water was gotten by the UV-vis spectral measurement. © 2011 The Royal Society of Chemistry.

Kuklik V.,Czech University of Life Sciences | Hoang T.D.,Hanoi University of Agriculture
Agricultural Water Management | Year: 2014

The main objective of this paper is determination of principal relationships influencing the distribution of moisture content in a soil profile under an emitter in point irrigation. Research was carried out by conducting field experiments. They determined geometry of wetted soil volume in a soil profile under point irrigation. Infiltration of water from a single emitter and the resulting spatial distribution is a typical feature of localized irrigation technology. The field experiments using different emitter discharges and various periods of irrigation on haplic Luvisol (ha LV) (ALFISOLS Udalfs) on loess were conducted in a soil profile 1. m deep.Admissible duration of irrigation in relation to discharge rates has been calculated and illustrated in a graph. Incorrect irrigation practices can cause serious environmental damage. A method for determining the width and depth of the wetted soil volume under the point irrigation was developed. This method is rapid and simple, and it gives consistent results. © 2013 Elsevier B.V.

Nguyen D.T.,Ghent University | Nguyen D.T.,Hanoi University of Agriculture | Vangansbeke D.,Ghent University | Lu X.,Guangdong Entomological Institute | de Clercq P.,Ghent University
BioControl | Year: 2013

Development, survival and reproduction of the predatory mite Amblyseius swirskii (Athias-Henriot) (Acari: Phytoseiidae) were assessed when fed on cattail pollen (Typha latifolia L.), dried fruit mite (Carpoglyphus lactis L.), or on two artificial diets. The basic artificial diet (AD1) was composed of honey, sucrose, tryptone, yeast extract, and egg yolk. This diet was enriched (AD2) by adding hemolymph from oak silkworm pupae (Antheraea pernyi (Guérin-Méneville)). Mites fed on C. lactis and AD2 had shorter immature and preoviposition periods than those fed on the other diets. The total number of deposited eggs was significantly higher for females fed on AD2 than for those maintained on the other diets. The intrinsic rate of increase (rm) of A. swirskii was highest on AD2 and C. lactis, followed by T. latifolia pollen, and AD1. In conclusion, the artificial diet AD2 supported development and reproduction of A. swirskii to the same extent as a factitious prey which is routinely used in the mass rearing of the phytoseiid. Our findings indicate the potential of artificial diets for the mass production of this economically important predatory mite. © 2012 International Organization for Biological Control (IOBC).

Tran D.T.,Catholic University of Leuven | Tran D.T.,Hanoi University of Agriculture | Knez K.,Catholic University of Leuven | Janssen K.P.,Catholic University of Leuven | And 4 more authors.
Biosensors and Bioelectronics | Year: 2013

The rising prevalence to food allergies in the past two decades, together with the fact that the only existing therapy is avoidance of allergen-containing food next to the implementation of anti-allergic drugs, urges the need for improved performance of current assays to detect potential allergens in food products. Therein, the focus has been on aptamer-based biosensors in recent years. In this paper we report for the first time the selection of aptamers against one of the most important peanut allergens, Ara h 1. Several Ara h1 DNA aptamers were selected after eight selection rounds using capillary electrophoresis (CE)-SELEX. The selected aptamers specifically recognized Ara h 1 and did not significantly bind with other proteins, including another peanut allergen Ara h 2. The dissociation constant of a best performing aptamer was in the nanomolar range as determined independently by three different approaches, which are surface plasmon resonance, fluorescence anisotropy, and capillary electrophoresis (353±82. nM, 419±63. nM, and 450±60. nM, respectively). Furthermore, the selected aptamer was used for bioassay development on a home-built fiber optic surface plasmon resonance (FO-SPR) biosensor platform for detecting Ara h 1 protein in both buffer and food matrix samples demonstrating its real potential for the development of novel, more accurate aptamer-based biosensors. In conclusion, the reported aptamer holds a great potential for the detection of Ara h 1 in both the medical field and the food sector due to its high affinity and specificity for the target protein. © 2012 Elsevier B.V.

Nguyen D.T.,Ghent University | Nguyen D.T.,Hanoi University of Agriculture | Vangansbeke D.,Ghent University | De Clercq P.,Ghent University
Experimental and Applied Acarology | Year: 2014

The generalist predatory mite Amblyseius swirskii Athias-Henriot (Acari: Phytoseiidae) was reared on Ephestia kuehniella Zeller eggs (Lepidoptera: Pyralidae), decapsulated dry cysts of the brine shrimp Artemia franciscana Kellogg (Anostraca: Artemiidae), and on meridic artificial diets (composed of honey, sucrose, tryptone, yeast extract, and egg yolk) supplemented with pupal hemolymph of the Chinese oak silkworm Antheraea pernyi (Guérin-Méneville) (Lepidoptera: Saturniidae) (AD1), with E. kuehniella eggs (AD2) or with A. franciscana cysts (AD3). Development, reproduction and predation capacity of the predatory mites were assessed in the first (G1) and sixth generation (G6) of rearing on the different diets. Immature survival rates in G1 were similar on all diets (96.8-100 %). After six generations, however, survival of A. swirskii was significantly reduced on all diets except on A. franciscana cysts. Oviposition rates did not differ between generations when females were fed on E. kuehniella, AD2 or AD3. The total number of deposited eggs was similar among diets except in G6 where the females fed on A. franciscana cysts produced more eggs than those maintained on E. kuehniella eggs. On most diets the intrinsic rates of increase in G1 were superior to those in G6, except for predators supplied with A. franciscana cysts where no differences were observed among generations. Female mites did not lose their capacity to kill first instar Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) after six generations on the different diets, but predation rates in G6 on E. kuehniella were lower than in G1. In conclusion, the different factitious and artificial diets tested in the present study supported the development and reproduction of A. swirskii for a single generation but fitness losses occurred to a varying degree after several generations on E. kuehniella eggs or the artificial diets. Artificial diet enriched with A. franciscana cysts yielded better results than the other artificial diets. Amblyseius swirskii performed best on decapsulated Artemia cysts indicating their potential for use in the mass production of the predator or to sustain its populations in the crop after release. © 2013 Springer Science+Business Media Dordrecht.

Thai T.H.,University of Miyazaki | Thai T.H.,Hanoi University of Agriculture | Yamaguchi R.,University of Miyazaki
Journal of Food Protection | Year: 2012

A total of 118 Salmonella isolates were detected from 283 retail meat samples (135 pork and 148 chicken meat) purchased at retail markets in Northern Vietnam. Thirteen serovars, including Infantis, Anatum, Rissen, Reading, Emek, Typhimurium, Blockley, London, Newport, Derby, Weltevreden, Albany, and Hadar, were determined. Resistance to tetracycline (54.2%), sulfonamides (52.5%), streptomycin (41.5%), trimethoprim (36.4%), chloramphenicol (35.6%), and ampicillin (33.1%) was commonly seen in the Salmonella isolates. Fourteen [blaTEM, blaOXA-1, blaPSE-1, aadA1, sul1, tetA, tetB, tetG, cmlA1, floR, dfrA1, dfrA12, aac(3)-IV, and aphA1-1AB] of 17 resistance genes were detected from the isolates demonstrating resistance. Genes for plasmid-mediated quinolone resistance, such as qnrA, qnrB, qnrS, qepA, and acc(6′)-1b-cr, were not detected in 23 quinoloneresistant isolates. The substitution TCC to TTC at codon 83 of gyrA was found in the 18 quinolone-resistant isolates. The data revealed that resistant Salmonella strains were widely distributed in Northern Vietnam via the food chain and that they might contain multiple genes specifying identical resistance phenotypes. Thus, further studies are necessary to clarify the mechanisms of antibiotic resistance in Salmonella strains and their spread in the livestock market. Copyright © International Association for Food Protection.

Thai T.H.,University of Miyazaki | Thai T.H.,Hanoi University of Agriculture | Hirai T.,University of Miyazaki | Lan N.T.,Hanoi University of Agriculture | Yamaguchi R.,University of Miyazaki
International Journal of Food Microbiology | Year: 2012

The spread of antibiotic resistance via meat poses a serious public health concerns. During 2007-2009, a total of 586 retail meat samples (318 pork and 268 chicken meats) were collected from three provinces (Bac Ninh, Ha Noi and Ha Tay) of North Vietnam to determine the prevalence of Salmonella. Isolates were characterized by serotyping and antibiotic susceptibility testing. Approximately 39.6% (n = 126) of pork and 42.9% (n = 115) of chicken samples were Salmonella-positive, and 14 Salmonella serovars were identified. Anatum (15.8%) was the most common serovar, followed by Infantis (13.3%), Emek (10.4%), Derby and Rissen (9.5%), Typhimurium (9.1%), Reading (7.5%) and London (6.2%). The isolation frequency of serovars Enteritidis, Albany, Hadar, Weltevreden, Newport and Blockey ranged from 1.2%-5.8%. Resistance to at least one antibiotic agent was detected in 78.4% of isolates (n = 189) and the most frequent resistance were to tetracycline (58.5%), sulphonamides (58.1%), streptomycin (47.3%), ampicillin (39.8%), chloramphenicol (37.3%), trimethoprim (34.0%) and nalidixic acid (27.8%). No Salmonella isolates were resistant to ceftazidime. Chicken isolates had higher resistance to antibiotic agents than pork isolates (P< 0.05). It showed that 159 Salmonella isolates belong to the 14 serovars were multidrug resistant (MDR) and 50 MDR patterns were found. This study indicated that Salmonella serovars isolated from retail meat samples were resistant to multiple antibiotics and this resistance was widespread among different serovars. The widespread resistance may have arisen from misuse or overuse of antibiotics during animal husbandry in North Vietnam. © 2012 Elsevier B.V.

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