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Xu N.,Zhejiang University | Xu N.,Zhejiang University of Technology | Wei X.,Zhejiang University | Ren B.,Hangzhou Zhongmei Huadong Pharmaceutical Co. | And 2 more authors.
Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis | Year: 2012

Based on near-infrared spectroscopy, four characteristic wavebands 4277.63~43166.20, 4887.06~4941.07, 5056.78~5172.50 and 5218.78~5303.64 cm -1, and two characteristic wavebands 4902.49~4817.64 and 4740.49~4 107.91 cm -1 were chosen to establish the partial least squares (PLS) regression model of water and adenosine in fermentation cordyceps powder, respectively. The prediction results of water and adenosine contents of the whole spectra PLS model were as follows: correlation coefficients (r) were 0.8683 and 0.7882, RMS error predictions (RMSEP) were 0.001999 and 0.000134, the remaining prediction deviations (RPD) were 1.9744 and 1.6407, respectively. However, using characteristic wavebands modeling can achieve a better performance with r of 0.8691 and 0.8290, RMSEP of 0.001934 and 0.001250, and RPD of 2.0407 and 1.8476 for water and adenosine respectively, and can largely improve calibration speed, providing the theoretical basis for the development of the testing instruments. So choosing the characteristic wavebands in this work to determine the water and adenosine in fermentation cordyceps powder is more effective.


Chen H.,China Pharmaceutical University | Chi X.,China Pharmaceutical University | Chi X.,Hangzhou Zhongmei Huadong Pharmaceutical Co. | Li B.,University of Washington | And 4 more authors.
Biomaterials Science | Year: 2014

In this study, gold nanorods (AuNRs) were first stabilized by hexadecyltrimethylammonium bromide (CTAB) and then coated with two kinds of polyelectrolytes (PE) and BSA to obtain multi-layered AuNRs (AuNRs-PE-BSA). Furthermore, the anti-cancer drug doxorubicin (DOX) was encapsulated into AuNRs-PE-BSA by the electrostatic force and the nanocomposites formed were named AuNRs/DOX-PE-BSA. The success of coating was verified by transmission electron microscopy (TEM), zeta potential, gel-electrophoresis and thermogravimetric analysis (TGA). The MTT assay indicated that the cytotoxicity of AuNRs decreased dramatically after multi-layer capping. The time-dependent nucleus-targeting capability of AuNRs/DOX-PE-BSA was confirmed in cell affinity evaluations. The in vitro and in vivo experiments demonstrated that AuNRs/DOX-PE-BSA, which combined photothermal and chemotherapy for tumor therapy, bears a markedly improved curative effect and holds promising prospects in the field of nanomedicine. © 2014 the Partner Organisations.


Chen H.,China Pharmaceutical University | Chen Y.,China Pharmaceutical University | Chen Y.,Hangzhou Zhongmei Huadong Pharmaceutical Co. | Yang H.,China Pharmaceutical University | And 5 more authors.
Polymer Chemistry | Year: 2014

Conventional chemotherapy suffers from non-specificity, lack of aqueous solubility and multidrug resistance. Tumor-targeting nanotherapeutics exhibit unique advantages in the delivery of drugs specifically into tumors. Here, folic acid (FA), methionine (Met) and a near infrared (NIR) fluorescence probe (cypate, ICG derivative) were all bio-conjugated to succinyl-chitosan (SC) micelles. An anti-cancer drug (paclitaxel, PTX) was loaded into the hydrophobic cores of the formed FA-Met-SC-ICG derivative (FMSCI) micelles, which were under 200 nm in size. In comparison with micelles containing a single targeting moiety (FA or Met), FA and Met co-mediated micelles presented excellent biocompatibility, much higher affinity for cancer cells and excellent tumor-specific distribution in tumor-bearing mice. In vivo anti-tumor activity demonstrated that PTX-loaded FMSCI provided favourable therapeutic efficacy for tumors. In this research, novel nanotherapeutics based on FMSCI loaded with an anti-cancer drug provide a promising nanocomposite for combined tumor-targeting imaging and therapy. © the Partner Organisations 2014.


Fu L.-F.,Hangzhou Zhongmei Huadong Pharmaceutical Co. | Tao Y.,Zhejiang University | Jin M.-Y.,Hangzhou Zhongmei Huadong Pharmaceutical Co. | Jiang H.,Zhejiang University
Biotechnology Letters | Year: 2016

Synthetic biology has been applied to direct improvement of valuable metabolite productions. Tacrolimus (FK506), a clinically used immunosuppressive agent isolated from many Streptomyces, is produced by fermentation in industry. Here we chose FK506 as an example to review recent progress in improving FK506 production, including enhancing transcription levels of biosynthetic genes, accelerating post-translational modification levels of biosynthetic enzymes, increasing activities of rate limiting enzymes, and rational supplement of limited precursors. FK506 production was increased from 25 % to sevenfold by these synthetic biology approaches. © 2016 Springer Science+Business Media Dordrecht


Patent
Huazhong University of Science, Technology and Hangzhou Zhongmei Huadong Pharmaceutical Co. | Date: 2014-01-08

Provided in the present invention is a pharmaceutical use of aminothiazole MyD88 specificity inhibitor, for use in preparing an immunosuppressant medicament, an anti-inflammatory medicament, a protection agent for post-ischemia reperfusion injury, and a preventative medicament for endotoxemia and sepsis.


Patent
Hangzhou Zhongmei Huadong Pharmaceutical Co., Huazhong University of Science and Technology | Date: 2013-09-01

2-aminothiazole derivatives represented by formula (I), where R_(1 )and R_(2 )represent cycloalkyls, respectively; or R_(1 )represents a substituted aromatic group, and R_(2 )represents H, a C_(1)-C_(11 )alkyl, CH_(2)Ph (benzyl), or a methyl ether including a C_(1)-C_(11 )alkyl. R_(3 )is a substituent including an amino group. X represents a carbonyl or a methylene and n is an integer from 0 to 5.


PubMed | Hangzhou Zhongmei Huadong Pharmaceutical Co. and Zhejiang University
Type: Review | Journal: Biotechnology letters | Year: 2016

Synthetic biology has been applied to direct improvement of valuable metabolite productions. Tacrolimus (FK506), a clinically used immunosuppressive agent isolated from many Streptomyces, is produced by fermentation in industry. Here we chose FK506 as an example to review recent progress in improving FK506 production, including enhancing transcription levels of biosynthetic genes, accelerating post-translational modification levels of biosynthetic enzymes, increasing activities of rate limiting enzymes, and rational supplement of limited precursors. FK506 production was increased from 25% to sevenfold by these synthetic biology approaches.


Patent
Huazhong University of Science, Technology and Hangzhou Zhongmei Huadong Pharmaceutical Co. | Date: 2014-01-08

The present invention relates to the preparation of a 2-aminothiazole derivative having a structure as formula (I) and a therapeutic effect thereof for Alzheimers disease (AD), and a therapeutic effect thereof against transplant rejection, autoimmune diseases, ischemia-reperfusion injury, chronic inflammation response, endotoxemia, and other diseases.


Patent
Hangzhou Zhongmei Huadong Pharmaceutical Co., Huazhong University of Science and Technology | Date: 2013-09-01

A method of treating an immunologic disease. The method includes administering an aminothiazole-based inhibitor of myeloid differentiation protein 88 (MyD88) to a patient in need thereof. The inhibitor of MyD88 acts as an immunomodulator for treatment of the immunologic disease including but not limited to rejections after organ transplantation, chronic inflammatory diseases, autoimmune diseases, ischemia-reperfusion injuries, and endotoxemia and sepsis.


PubMed | Hangzhou Zhongmei Huadong Pharmaceutical Co. and Zhejiang University
Type: Journal Article | Journal: Protein and peptide letters | Year: 2016

Many polyketides show biological activities and have thus been applied in clinics, as food additives, and in agriculture. Type II thioesterases (TEIIs) play an important role in polyketide biosynthesis. Most TEIIs belong to /-hydrolase family and usually contain a catalytic triad Ser-His-Asp. In polyketide biosynthesis, TEIIs can play an editing role by removal of aberrant non-extendable acyl units in elongation steps, a starter unit selection role by removal of unfavored starter acyl units in initiation steps, and a releasing role by removal of final product in termination steps. Complementation of TEIIs has been observed and applied.

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