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Peng J.,Hangzhou Third Peoples Hospital | Feng W.,Hangzhou Third Peoples Hospital | Luo X.,Hangzhou Third Peoples Hospital | Wang T.,Hangzhou Third Peoples Hospital | And 4 more authors.
Medical Science Monitor | Year: 2017

Background: Squamous cell carcinoma (SCC) is the second most common type of skin cancer, for which non- or mini-invasive treatment is of critical importance. 5-aminolevulinic acids based photodynamic therapy (ALA-PDT) is a mini-invasive approach that causes focal tumor cell injury, apoptosis, and necrosis through light sensitivity. The efficacy of combining ALA-PDT and surgery in treating SCC, however, has not been demonstrated. Material/Methods: A total of 60 SCC patients were randomly assigned into attrition plus ALA-PDT group (experimental group) and single ALA-PDT treatment group (control group). Clinical efficacy, recurrence rate, and adverse effects were analyzed in conjunction with H&E staining and immunohistochemistry (IHC) staining for p53 expression. Results: The overall effective rate of the experimental group was 73.3%, which was significantly higher than that of the control group (46.7%). The experimental group also had a lower recurrence rate (16.6% versus 30.0%, p<0.05). Similar rates of adverse effects existed between the two groups. After treatment, abnormal cells disappeared, while the p53 positive rate after treatment was elevated in the two groups (p<0.05 comparison of before and after treatment). The experimental group had a higher p53 positive rate compared to the control group (p<0.05). Conclusions: Combined therapy of attrition with ALA-PDT significantly elevated the effective treatment rate and can decrease the recurrence rate with reliable safety in treating SCC, thus ALA-PDT can be used as an optimal plan for SCC treatment. © Med Sci Monit.


Zhong H.,Chongqing Medical University | Song Z.,Chongqing Medical University | Chen W.,TU Munich | Li H.,Chongqing Medical University | And 18 more authors.
Allergy: European Journal of Allergy and Clinical Immunology | Year: 2014

Background: The epidemiologic profiles of chronic urticaria (CU) vary considerably among regions, and few such data are available from China. Methods: We performed a multicenter open questionnaire investigation about the clinical and laboratory features of CU, defined as recurrent wheals with/without angioedema lasting for ≥6 weeks, among 3027 patients. Results: Female preponderance was observed (female/male ratio, 1.46: 1). The mean age at diagnosis was 34.7 ± 13.8 years, and the mean disease duration was 18.5 ± 46.1 months (range, 1.5-127 months). Patients were classified as having chronic spontaneous urticaria (CSU, 61.0% of patients), physical urticaria (PU, 26.2%), or other urticaria types (OU, 2.3%). Nocturnal attacks were reported by 60% of cases. The Urticaria Activity Score (UAS) in patients with CSU was 3.8 ± 1.4. The mean Dermatology Life Quality Index was 7.3 ± 3.4 (range 0-30). Induction or exacerbation of wheals with alcohol drinking was reported by 55.7% of patients. Chronic hepatitis B was less prevalent in our CU patients compared with the general Chinese population (2.7% vs 7%). Positive autologous serum skin tests (ASSTs) were observed in 66.9% of patients. Patients with positive ASST had higher UAS, greater angioedema frequencies, longer disease durations, and poorer QoL compared with patients with negative ASST (P < 0.05). Conclusion: In this Chinese population, CU usually affected youth, and CSU was the most common subtype. Autoreactivity and alcohol consumption were the top two triggers for CU, whereas latent infectious and chronic inflammatory diseases were not as common as in previous reports. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.


PubMed | Yuhang District First Peoples Hospital, Origin Biosciences Inc., Anticancer, Inc., P-Com and 3 more.
Type: Journal Article | Journal: In vivo (Athens, Greece) | Year: 2016

The traditional Chinese medicine Baishaoqiwu (BSQW) has been previously used to clinically treat inflammatory bowel diseases. However, the mechanisms of its therapeutic efficacy remain unclear. The aim of this study was to examine the efficacy of BSQW on ulcerative colitis (UC) and the TLR4/MyD88/NF-B signaling pathway in a rat model of colitis.The colitis rat model was induced by anal instillation of 2,4,6-trinitrobenzene sulfonic acid (TNBS). The animals with induced colitis were treated with BSQW at a dose of 13.2 mg/kg daily, p.o. Mesalazin was used as a positive control and was given to the animals with induced colitis at a dose of 420 mg/kg daily, p.o. The untreated animals with induced colitis and normal animals served as model controls and normal controls, respectively. Macroscopic and histological assessments were performed after treatment. The expression of MyD88, NF-B P65 and TLR4 were determined by immunohistochemical analysis.Administration of BSQW or Mesalazin ameliorated TNBS-induced macroscopic and histological damage in the rats with induced colitis. The macroscopic score and total colitis index were significantly reduced in the BSQW- and Mesalazin-treated groups compared to the model control group (p<0.05). BSQW or Mesalazin significantly inhibited TNBS-induced expression of the TLR4, MyD88 and NF-B P65 genes. No treatment-related toxicity was found in either the BSQW- or the Mesalazin-treated groups.Suppression of the TLR4/MyD88/NF-B signaling pathway may be one of the mechanisms involved in the therapeutic efficacy of BSQW against UC.


Wang Y.-Q.,Hangzhou Third Peoples Hospital | Liu Y.-X.,Hangzhou Third Peoples Hospital | Yang J.-W.,Hangzhou Third Peoples Hospital
Journal of Practical Oncology | Year: 2015

Objective: To evaluate the efficacy and safety of high-dose methotrexate (HD-MTX) combined with multi-drug chemotherapy for patients with ostetmarcoma. Methods: Clinical data of 63 ostetmarcoma patients treated by HD-MTX combined with multi-drug chemotherapy were retrospectively reviewed. Factors related to the survival and prognosis of patients were analyzed. Results: Three-year overall survival rate was 67.7% among 63 patients, while that for patients who completed 4 cycles of postoperative chemotherapy was 81.4% (P<0.05). Three-year overall survival rate was significantly related to tumor size, serum AKP level, surgery, complete chemotherapy course, chemotherapy efficacy, and distant metastasis (all P<0.05). Cox-model showed that surgery and complete chemotherapy course were independent prognostic factors (P<0.05). Conclusion: HD-MTX combined with multi-drug chemotherapy in treatment of osteosareoma is effective, which should be given based on surgery and complete therapeutic courses. ©, 2015, Journal of Practical Oncology, Editorial Board. All right reserved.


Lan X.-X.,Hangzhou Third Peoples Hospital
International Eye Science | Year: 2015

AIM: To understand the hospital infection and explore its risk factors in hospitalized patients with cataract. METHODS: The medical record information of 68 cases with hospital infection between January 2009 and December 2014 in our hospital were retrospectively analyzed. The risk factors of hospital infection in hospitalized patients were analyzed using screening cataract multiariable Logistic regression analysis. RESULTS: The incidence of hospital infection in hospitalized patients with cataract was 1.37%, respiratory tract infections had the highest incidence in 39 cases, accounting for 57.35%. The results of multi-factor Logistic regression analysis showed that: sex examination, invasive operation, hospitalization days were risk factors for hospital infection in hospitalized patients with cataract. CONCLUSION: Hospitalized cataract patients have risk of combined hospital infection, we should strengthen the hospital infection management, strict aseptic operation, for reducing the occurrence of hospital infection. Copyright 2015 by the IJO Press.


Hu Y.-P.,Hangzhou Third Peoples Hospital | Zhou H.-Y.,Zhejiang University | Shen S.,Jiangsu University
Yaoxue Xuebao | Year: 2016

Magnetic iron oxide (Fe3O4) nanoparticles were sythesized using solvothermal reaction and then coated with titanium oxide (TiO2) via sol-gel process of hydrolysis and condensation of tetrabutyl titanate (TBOT). The obtained Fe3O4-TiO2 particles were characterized with transmission electron microscope (TEM) and dynamic light scattering (DLS). The loading and release of doxorubicin (DOX) were evaluated. Methyl thiazolyl tetrazolium (MTT) method was used to study the cytotoxicity and effect of chemo-photodynamic therapy. The obtained Fe3O4-TiO2 particles were uniform and well dispersed. The loading capacity of DOX was 43%. A pH-sensitive release property of Fe3O4-TiO2-DOX was observed. In the cytotoxicity experiment, cytotoxicity was found upon combination of Fe3O4-TiO2-DOX and ultraviolet (UV), while no obvious cytotoxicity was found in the blank Fe3O4-TiO2 particles. In conclusion, the fabricated Fe3O4-TiO2 nanoparticles exhibited a high loading capacity and excellent photodynamic therapeutic effect, suggesting that it may be used as a novel carrier for chemo-photodynamic therapy of cancer.


Yan Y.,Hangzhou Third Peoples Hospital | Liang L.,Hangzhou Third Peoples Hospital | Chen T.,Hangzhou Third Peoples Hospital | Zhong C.,Hangzhou Third Peoples Hospital | Li P.,Hangzhou Third Peoples Hospital
Revista da Associacao Medica Brasileira | Year: 2012

Objective: To study the efficacy, safety, and feasibility of stent-assistant angioplasty (SAA) in the treatment of symptomatic vertebrobasilar artery stenosis in the elderly. Methods: SAA was performed in 26 elderly patients with symptomatic vertebrobasilar artery stenosis. The success rate, perioperative complications, and long-term effectiveness were evaluated. Results: A total of 29 balloon expandable stents were implanted in these patients. The success ratio was 100%. The degree of stenosis decreased from 81.3 ± 8.8% to 3.7 ± 3.6% (p < 0.01). Complications were absent during the perioperative period. Follow-up was performed for seven to 36 months (median: 21.9 months). Two patients developed the recurrent symptoms of vertebrobasilar artery stenosis, and no cerebral ischemic events were noted in the remaining patients, suggesting a favorable outcome. Conclusion: SAA is a safe and effective strategy for the treatment of symptomatic vertebrobasilar artery stenosis in the elderly. © 2012 Elsevier Editiora Ltda. All right reserved.


PubMed | Hangzhou Third Peoples Hospital
Type: Journal Article | Journal: Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery | Year: 2016

To investigate the function of repair gene LIG4 in radiosensitivity enhancement of rectal cancer cells by curcumin.Human rectal cancer cells HT-29 were cultured in normal. LIG4-overexpression HT-29 cells and blank control plasmid HT-29 cells were established by gene transfection. Both kind of HF-29 cells were further randomly divided into curcumin group, radiotherapy group, curcumin plus radiotherapy group (combined group) and control group. The growth inhibition and apoptosis of cells were detected by MTT and Annexin V/PI respectively. Change of tumor volume was observed in nude mouse xenograft model, and the apoptosis of tumor cells was analyzed by TUNEL.Regarding blank control plasmid HT-29 cells, the growth inhibition rate and apoptosis rate in combined group were significantly higher than those in radiotherapy group(all P<0.05); tumor volume of nude mouse in combined group was significantly smaller than that in radiotherapy group, and the apoptotic index in combined group was significantly higher than that in radiotherapy group (all P<0.05). However, regarding LIG4-overexpression HT-29 cells, the growth inhibition rate and apoptosis rate were not significantly different between combined group and radiotherapy group(all P>0.05); the tumor volume of nude mouse and the apoptotic index were also not significantly different between combined group and radiotherapy group (all P>0.05).Down-regulation of LIG4 is an important mechanism of radiosensitivity enhancement of rectal cancer cells by curcumin.


PubMed | Hangzhou Third Peoples Hospital
Type: Journal Article | Journal: Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery | Year: 2014

To evaluate the impact of transanal endoscopic microsurgery (TEM) on postoperative anal function and quality of life in patients with benign rectal tumor and early rectal cancer.Clinical data of 50 patients with rectal adenoma and early rectal cancer undergoing transanal endoscopic microsurgery in our hospital from October 2008 to June 2013 were retrospectively analyzed. Anorectal manometry, endorectal ultrasonography (ERUS), the fecal incontinence severity index (FISI), and the physical and mental health status scores (SF-36) were used to evaluate preoperative and postoperative anorectal function and quality of life.Anorectal manometry indicated anal resting pressure (ARP), maximum squeeze pressure (MSP), rectal volume at sensory threshold(RVST), maximum tolerable volume(MTV) decreased significantly at the first month after surgery (P<0.05). MSP returned to preoperative level at the 3rd month (P>0.05). ARP and MTV returned to normal values at the 6th month (P>0.05). RVST returned to normal values at the 9th month (P>0.05). Recto-anal inhibitory reflex(RAIR) was absent in 1 (2%) patient preoperatively and in 30(60%), 18(36%), 7(14%), 2(4%) at the 1st, 3rd, 6th, 9th months after surgery respectively. ERUS showed similar width and thickness of internal sphincter at 1st and 6th month after surgery compared with preoperative measures (P>0.05). Six months after surgery, the mean FISI score decreased(preoperative vs postoperative:8.5 vs 5.8, P<0.05), suggesting an improvement in fecal continence. However, the overall quality of life did not danger significantly after surgery(P>0.05).TEM has little impact on anorectal anatomic structure. Anal function may be compromised in the short-term, however the vast majority of patients recover completely after 6-9 months. TEM is a safe, effective and minimally invasive surgery.


PubMed | Hangzhou Third Peoples Hospital
Type: Evaluation Studies | Journal: Revista da Associacao Medica Brasileira (1992) | Year: 2012

To study the efficacy, safety, and feasibility of stent-assistant angioplasty (SAA) in the treatment of symptomatic vertebrobasilar artery stenosis in the elderly.SAA was performed in 26 elderly patients with symptomatic vertebrobasilar artery stenosis. The success rate, perioperative complications, and long-term effectiveness were evaluated.A total of 29 balloon expandable stents were implanted in these patients. The success ratio was 100%. The degree of stenosis decreased from 81.3 8.8% to 3.7 3.6% (p < 0.01). Complications were absent during the perioperative period. Follow-up was performed for seven to 36 months (median: 21.9 months). Two patients developed the recurrent symptoms of vertebrobasilar artery stenosis, and no cerebral ischemic events were noted in the remaining patients, suggesting a favorable outcome.SAA is a safe and effective strategy for the treatment of symptomatic vertebrobasilar artery stenosis in the elderly.

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