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Song Y.,Zhejiang University of Technology | Xiao Y.,Zhejiang University of Technology | Chen Q.,Third hospital of Hangzhou
Critical Reviews in Eukaryotic Gene Expression | Year: 2014

The mitochondrion is the site of energy metabolism inside the cell; which produces ATP for vital activities. The accumulation of damaged mitochondria affects cell survival, these organelles are removed via mitophagy to maintain cell vitality. Currently, mitophagy is a hot topic in life science research. Understanding the role of mitophagy in pathogenesis and its association with the genesis of disease is important for developing treatments for many human diseases. Here, we summarize current progress in the study of mitophagy and describe the different molecular mechanisms of mitophagy between yeast and mammals, as well as related diseases. © 2014 Begell House, Inc.


PubMed | Third hospital of Hangzhou and Zhejiang University of Technology
Type: Journal Article | Journal: Critical reviews in eukaryotic gene expression | Year: 2014

The mitochondrion is the site of energy metabolism inside the cell; which produces ATP for vital activities. The accumulation of damaged mitochondria affects cell survival, these organelles are removed via mitophagy to maintain cell vitality. Currently, mitophagy is a hot topic in life science research. Understanding the role of mitophagy in pathogenesis and its association with the genesis of disease is important for developing treatments for many human diseases. Here, we summarize current progress in the study of mitophagy and describe the different molecular mechanisms of mitophagy between yeast and mammals, as well as related diseases.


Huang B.,Zhejiang University | Huang B.,First Hospital of Jiaxing | Sun K.,Zhejiang University | Zhu Z.,First Hospital of Jiaxing | And 4 more authors.
Clinical Radiology | Year: 2013

Aim To test the hypothesis that the oximetry-derived perfusion index (PI) recorded in the index finger may provide earlier objective evidence for correct positioning of the needle tip during computed tomography (CT)-guided thoracic sympathetic blockade than skin temperature in palmar hyperhidrosis. Materials and methods Forty-four CT-guided thoracic sympathetic blockades were prospectively performed in both hands of 22 patients. Prior to chemical blockade, PI and skin temperature were recorded at 1 min intervals until 20 min after lidocaine injection. Repeated measures analysis of variance was used to assess the potency of PI and skin temperature over time. Using a 100% increase in the PI as the threshold and symptom relief within 20 min as the reference standard, the sensitivity and specificity for predicting a successful injection were calculated. Results Thirty-seven (84%) sympathetic blockade procedures were clinically successful. For successful cases, the PI increased as early as 1 min after the blockade of the sympathetic chain (p < 0.05), whereas the skin temperature showed statistical significance 1 min later (p < 0.05). The PI can be used to indicate a successful CT-guided sympathetic blockade with 97% sensitivity and 100% specificity 3 min after lidocaine injection. Conclusion The PI, a more marked and sensitive indicator than changes in skin temperature, can be used to indicate a successful CT-guided sympathetic blockade with satisfying sensitivity and specificity, 3 min after lidocaine injection in palmar hyperhidrosis. © 2013 The Royal College of Radiologists. Published by Elsevier Ltd. All rights reserved.


Xiang W.Z.,Third Hospital of Hangzhou | Xu A.E.,Third Hospital of Hangzhou | Xu J.,Third Hospital of Hangzhou | Bi Z.G.,Nanjing Medical University | And 2 more authors.
Laser Physics | Year: 2010

Confocal laser scanning microscopy (CLSM) allows noninvasive visualization of human skin in vivo, without needing to fix or section the tissue. Melanocytes and pigmented keratinocytes at the level of the basal layer form bright dermal papillary rings which are readily amenable to identify in confocal images. Our purpose was to explore the role of dermal papillary rings in assessment of lesion location, the diagnosis, differential diagnosis of lesions and assessment of therapeutic efficacy by in vivo CLSM. Seventy-one patients were imaged with the VivaScope 1500 reflectance confocal microscope provided by Lucid, Inc. The results indicate that dermal papillary rings can assess the location of lesion; the application of dermal papillary rings can provide diagnostic support and differential diagnosis for vitiligo, nevus depigmentosus, tinea versicolor, halo nevus, common nevi, and assess the therapeutic efficacy of NBUVB phototherapy plus topical 0.1 percent tacrolimus ointment for vitiligo. In conclusion, our findings indicate that the dermal papillary rings play an important role in the assessment the location of lesion, diagnosis, differential diagnosis of lesions and assessment of therapeutic efficacy by in vivo CLSM. CLSM may be a promising tool for noninvasive examination in dermatology. However, larger studies are needed to expand the application of dermal papillary rings in dermatology. © 2010 Pleiades Publishing, Ltd.


PubMed | Third Hospital of Hangzhou, The Second Peoples Hospital of Weifang, Shandong University and Zhejiang Chinese Medical University
Type: Journal Article | Journal: Acta biochimica et biophysica Sinica | Year: 2016

Flavonoids have been shown to improve cognitive function and delay the dementia progression. However, the underlying mechanisms remain elusive. In the present study, we examined the effect of Scutellaria baicalensis stem-leaf total flavonoids (SSTFs) extracted from S. baicalensis Georgi on spatial learning and memory in a vascular dementia (VaD) rat model and explored its molecular mechanisms. The VaD rats were developed by permanent bilateral occlusion of the common carotid artery. Seven days after recovery, the VaD rats were treated with either 50 or 100 mg/kg of SSTF for 60 days. The spatial learning and memory was evaluated in the Morris water maze (MWM) test. The tau hyperphosphorylation and the levels of the related protein kinases or phosphatases were examined by western blot analysis. In VaD rats, SSTF treatment at 100 mg/kg significantly reduced the escape latency in training trial in MWM test. In the probe trial, SSTF treatment increased the searching time and travel distance in the target quadrant. SSTF treatment inhibited the tau phosphorylation in both cortex and hippocampus in VaD rats. Meanwhile, SSTF reduced the activity of glycogen synthase kinase 3 and cyclin-dependent kinase 5 in VaD rats. In contrast, SSTF treatment increased the level of the protein phosphatase 2A subunit B in VaD rats. SSTF treatment significantly improved the spatial cognition in VaD rats. Our results suggest that SSTF may alleviate tau-hyperphosphorylation-induced neurotoxicity through coordinating the activity of kinases and phosphatase after a stroke. SSTF may be developed into promising novel therapeutics for VaD.


Xiang W.,Third Hospital of Hangzhou | Xu A.,Third Hospital of Hangzhou | Xu J.,Third Hospital of Hangzhou | Bi Z.,Nanjing Medical University | And 2 more authors.
Lasers in Medical Science | Year: 2010

The use of confocal laser scanning microscopy (CLSM) may be an eligible alternative for confirmation of the diagnosis of hypopigmented macules. Our purpose was to evaluate CLSM features for non-invasive imaging of vitiligo, nevus depigmentosus and postinflammatory hypopigmentation in vivo. A total of 68 patients with a clinical diagnosis of the aforementioned diseases were included in this study. CLSM was performed on lesional and adjacent normal appearing skin for all patients. In the active and stable phases of vitiligo, CLSM demonstrated a complete loss of melanin in lesional skin in 14 of 25 patients (56.0%) and 16 of 20 patients (80.0%), respectively. In 11 of 25 (44.0%) patients, the amount of melanin in lesional skin decreased in the active phase of vitiligo, but it is noteworthy to know that the melanin was distributed homogeneously in the dermal papillary rings. In four of 20 patients (20.0%), the dermal papillary rings disappeared completely, but some refractile granules and dendrites could be seen in the stable phase of vitiligo, which may indicate the start of vitiligo repigmentation. Although, in 20 of 20 patients (100%) with nevus depigmentosus, the dermal papillary rings lost their integrity and the content of melanin decreased obviously, there must have been melanin in the dermal papillary rings during its development in all patients. Simultaneously, the melanin was distributed heterogeneously in the dermal papillary rings. The content of melanin and dermal papillary rings in postinflammatory hypopigmentation probably depend on the depth and site of the inflammation; moreover, melanophages were observed in postinflammatory hypopigmentation but did not exist in vitiligo and nevus depigmentosus. In addition, the content of melanin and dermal papillary rings in adjacent normal appearing skin showed changes in the active phase of vitiligo but showed no changes in any of the patients in the stable phases of vitiligo, nevus depigmentosus and postinflammatory hypopigmentation. Differences based on CLSM in the aforementioned diseases were the content of melanin and its distribution pattern. CLSM may be useful to discriminate vitiligo, postinflammatory hypopigmentation and nevus depigmentosus in a non-invasive fashion. © Springer-Verlag London Ltd 2010.


Liu J.F.,Third Hospital of Hangzhou
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology | Year: 2010

To explore the inhibition effect of RNA interference on the ICP4 expression and DNA replication of herpes simplex virus type 2 (HSV2). Four pairs of siRNA targeted to HSV2 ICP4 gene and negative control siRNA were synthetized by chemical method, named as siRNA-1, siRNA-2, siRNA-3, siRNA-4 and siRNA-N respecticely. HSV2 HG52 was used to attack Vero cell after transfection overnight. Vero cell and supernatant were collected at 1d, 2d, 3d, 4d and 5d after virus attacking. Flurogenic quantitative reverse transcription polymerase chain reaction (FQ-RT-PCR)was used to detect the expression of HSV2 ICP4 mRNA, flurogenic quantitative polymerase chain reaction(FG-PCR) was used to detect the expression of HSV2 DNA and Western-Blot was used to detect the expression of HSV2 ICP4 protein. All the four pairs of siRNA could significantly inhibit the expression of HSV2 ICP4 mRNA and protein, especially siRNA-2. The above siRNAs could significantly decrease HSV2 DNA copy number,too. siRNAs targeted to HSV2 ICP4 gene could significantly inhibit expression of HSV2 ICP4 mRNA and protein, and decrease HSV2 DNA copy number, suggesting that siRNA can inhibit HSV2 DNA replication through silencing ICP4 gene.


Lu N.,Third Hospital of Hangzhou | Xu A.,Third Hospital of Hangzhou | Wu X.,Third Hospital of Hangzhou
Journal of Dermatological Treatment | Year: 2014

Background: Autologous epidermal transplantation is available for the treatment of stable vitiligo. However, the results of this technique are affected by many factors. Objectives: It is important to investigate the long-term results and factors that might influence the outcome of the autologous epidermal grafting technique to form a basis for guidance for patients with vitiligo. Methods: We performed a follow-up study involving 173 patients (95 male, 78 female) with vitiligo vulgaris and 109 patients (59 male, 50 female) with segmental vitiligo who underwent autologous epidermal grafting. We investigated the long-term percentage of repigmentation and colour matching up to 13 years after treatment (mean 1.87 years). Results: The mean percentage of repigmentation in the total of 1938 sheets was 80.65%. In patients with vitiligo vulgaris, 110 (63.6%) showed excellent repigmentation, 18 (10.4%) showed good, 4 (5.8%) showed fair and 12 (20.2%) showed poor repigmentation. In patients with segmental vitiligo, 83 (76%) showed excellent repigmentation, 10 (9%) showed good, 4 (4%) showed fair and 12 (11%) showed poor repigmentation. In segmental vitiligo, the degree of repigmentation was significantly higher than that in vitiligo vulgaris lesions. Better outcomes were obtained in female patients and less than 20 years old patients, whereas results in male patients and more than 20 years old were inferior in both vitiligo vulgaris and segmental vitiligo patients. The face and neck had a higher completely matching ratio in the treated area with the surrounding skin compared to the non-exposed sites. However, the face and neck had a lower repigmentation in vitiligo vulgaris. There was a higher ratio of Koebner phenomenon on the donor site and new lesions on the other sites in 45 no repigmentation patients. Conclusions: Autologous epidermal transplantation achieves a high percentage of repigmentation which is affected by sex, age, types, position, duration and the stability time before transplantation in vitiligo patients. A more perfect colour matching was obtained in exposure sites. Disease activity after transplantation was very important for repigmentation or not. © 2014 Informa Healthcare USA on behalf of Informa UK Ltd.


PubMed | Third Hospital of Hangzhou
Type: Case Reports | Journal: Clinical and experimental dermatology | Year: 2015

We describe a case of primary cutaneous mucormycosis in a 44-year-old man with an 18-month history of infiltrative erythematous plaques and haemorrhagic crusting on the dorsum of his left hand. The isolate was identified as Mucor irregularis (formerly Rhizomucor variabilis) based on the fungus morphology and DNA sequencing results. Improvement was observed after a 6-month treatment course of itraconazole. No recrudescence was seen during a follow-up of 23 months after treatment.


PubMed | Third Hospital of Hangzhou
Type: Journal Article | Journal: The Journal of dermatology | Year: 2015

We describe a case of cutaneous Penicillium marneffei infection in a non-HIV-infected male patient with idiopathic CD4(+) T lymphocytopenia (ICL). The cutaneous lesions were cured after the treatment of itraconazole combined with interleukin-2.

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