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Hangzhou, China

Xu Y.-F.,Hangzhou Third Hospital | Jiang S.-J.,Hangzhou Third Hospital | Wang Y.-Q.,Hangzhou Third Hospital | Yao Y.-W.,Hangzhou Third Hospital | Liu Y.-X.,Hangzhou Third Hospital
International Journal of Clinical and Experimental Medicine | Year: 2016

Objective: To evaluate the effect and security of high-dose methotrexate (HD-MTX) multidrug first-line chemotherapy on primary osteosarcoma in regions near the knee joint, as well as its effect on the patients survival and the prognosis-related clinical features. Methods: A retrospective analysis was performed on the data collected from the Hangzhou Third Hospital during June 2007 to June 2012. These patients were treated with HD-MTX/HD-MTX/DDP (cisplatin)/ADM (doxorubicin) and/or HD-MTX/IFO (Ifosfamide)/DDP/ADM, and surgery respectively. The treatment effects were followed up. Kaplan-Meier method was adopted for survival analysis and the survival curve was graphed. Log-Rank test was applied for single factor analysis while COX’s Proportional Hazard Model was used for multi-variate analysis. Result: The average follow-up duration was 51.14 months. The follow-up rate was 91.6%. 117 cases received neoadjuvant chemotherapy. No case had complete response (CR). Partial response (PR), stable disease (SD) and disease progression (PD) were seen in 82, 20 and 15 patients respectively. The objective response rate (RR) was 70.08% (82/117). The disease control rate (DCR) was 87.18% (102/117). The 3-year event-free survival, overall survival, local recurrence and distant metastasis were 67.94%, 83.21%, 6.11% and 19.08%, respectively. Improvements has been seen in 103 patients who completed 4-cycle chemotherapy, whose 3-year event-free survival, overall survival, local recurrence and distant metastasis were 72.82%, 84.47%, 2.91% and 17.48%. Standard chemotherapy can significantly (P<0.05) improve patients prognosis including 3-year survival, event-free survival, 3-year local recurrence and distant metastasis. Univariate analysis showed that survival is significantly related to age, pathologic fracture, standard chemotherapy, histological response, orthopedic surgical options, and distant metastasis (P<0.05), among which age, pathologic fracture and histological response are independent factors that related to prognosis. Conclusion: HD-MTX multidrug therapy has both satisfying short-term and long-term effects. The side effect was controllable. Patients following this regimen showed a good level of compliance. Therefore, this regimen is highly recommended. Having an optimized and new regiment through multi-discipline collaborations is the key to a better osteosarcoma treatment. © 2016, E-Century Publishing Corporation. All rights reserved. Source

Liao X.-J.,Hangzhou Third Hospital | Mao W.-M.,Hangzhou Third Hospital | Wang Q.,Hangzhou Third Hospital | Yang G.-G.,Hangzhou Third Hospital | And 2 more authors.
Biochemical and Biophysical Research Communications | Year: 2016

Irritable bowel syndrome (IBS) is a common chronic functional gastrointestinal disorder. MicroRNAs (miRNAs) have been widely demonstrated to take part in various physiological and pathological processes. In the present study, the role of miR-24 in the pathogenesis of IBS and the potential mechanism in this process were evaluated. Human intestinal mucosa epithelial cells of colon from IBS patients and healthy subjects were collected. An IBS mouse model was established with the induction of trinitro-benzene-sulfonic acid (TNBS). The expression levels of miR-24 and serotonin reuptake transporter (SERT) were analyzed using Real-time PCR and western blot in both human specimen and mice. miR-24 was upregulated in IBS patients and mice intestinal mucosa epithelial cells. Luciferase reporter assay showed that SERT was a potential target gene of miR-24. The treatment of miR-24 inhibitor increased pain threshold and nociceptive threshold levels and reduced MPO activity in proximal colon of IBS mice, and up-regulated the mRNA and protein expression levels of SERT in intestinal mucosa epithelial cells. miR-24 played a role in the pathogenesis of IBS probably through regulating SERT expression. © 2015 Elsevier Inc. All rights reserved. Source

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