Time filter

Source Type

Yin D.,East China University of Science and Technology | Yin D.,Hangzhou Institution of Quality and Technical Supervision and Inspection | Wang N.,East China University of Science and Technology | Wang N.,Zhengzhou University of Light Industry | And 3 more authors.
European Journal of Soil Biology | Year: 2013

Pseudomonas fluorescens 2P24 and CPF10 are two biocontrol agents that were isolated from suppressive soils of wheat take-all disease in Shandong province, China. The main objective of this study was to assess how these strains impact the bacterial community in cucumber rhizosphere in the field over 56 days. Classical microbiological culturing and molecular tools based on denaturing gradient gel electrophoresis (DGGE) and terminal-restriction fragment length polymorphism (T-RFLP) were used in this study. The survival of P.fluorescens 2P24 and CPF10 in cucumber rhizosphere was monitored by using selective plating. The results showed that the concentration of P.fluorescens 2P24 and CPF10 decreased from ca. 107CFU/g to ca.103CFU/g in soil after inoculation. The compared data of T-RFLP and DGGE showed temporary changes in the bacterial community in the soil that was treated with the biocontrol agents. From the 7th to 42nd days after inoculation, the alterations of several specific bacterial groups (Cyanobacterium, Beta-proteobacterium, Staphylococcus, and Bacillus) were detected in the results of DGGE with cloning and sequencing. However, on the 56th day after inoculation, there was no prominent alteration of bacterial community. In general, effects of these two biocontrol agents on indigenous bacteria in cucumber rhizosphere were transient and generally similar.© 2013 Elsevier Masson SAS. Source

Yu S.,Henan University | Yuan J.,Zhengzhou University | Shi J.,Henan University | Ruan X.,Hangzhou Institution of Quality and Technical Supervision and Inspection | And 3 more authors.
Journal of Materials Chemistry B | Year: 2015

A small, nano-sized, water-soluble polyrotaxane (PR) was synthesized using a highly efficient one-pot synthesis strategy in a homogeneous water system, formed from β-cyclodextrin-(COOH)2, poly(propylene glycol)bis(2-aminopropyl ether) (PPG, 2 kDa) and a mono-(6-azido-6-desoxy)-β-cyclodextrin stopper via room temperature click chemistry. β-cyclodextrin-(COOH)2 and PR were characterized by one- and two-dimensional NMR as well as by high resolution transmission electron microscopy (HR-TEM). The number of carboxyl groups in one PR was determined by 1H NMR. Two-dimensional diffusion-ordered NMR spectroscopy (2D DOSY) and nuclear Overhauser enhancement spectroscopy (2D NOESY) show that β-cyclodextrin-(COOH)2 and PPG successfully formed an inclusion complex. HR-TEM revealed the morphology of water-soluble PR as a spherical nanoparticle with a size of approximately 3.5 nm ± 1.5 nm. PR was labeled with rhodamine to assess its biocompatibility and cell membrane penetrability in vitro. The in vivo real-time fluorescent imaging biodistribution experiments indicated that water-soluble PR can actively target tumor sites using an enhanced permeability and retention (EPR) effect, with a significantly prolonged blood circulation time in tumor-bearing mice. This journal is © The Royal Society of Chemistry. Source

Lin S.,Hangzhou Institution of Quality and Technical Supervision and Inspection | Tu H.,Hangzhou Institution of Quality and Technical Supervision and Inspection | Sun L.,Hangzhou Institution of Quality and Technical Supervision and Inspection | Xiao H.,Hangzhou Institution of Quality and Technical Supervision and Inspection | And 2 more authors.
Chinese Journal of Chromatography (Se Pu) | Year: 2011

A method based on high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was developed for simultaneous determination of five yellow industrial dyes. The separations were performed with an Agilent ODS C18 column at a flow rate of 0. 3 mL/min. The mobile phase was 5 mmol/L ammonium acetate (containing 0. 1% formic acid) -acetonitrile (3:2, v/v). Under the optimized detection conditions; the linear ranges for Chrysoidine G and Basic Yellow 2 were 5. 0 - 80. 0 mg/L, and for Acid Orange I, Acid orange II and Acid Yellow 36 were 10. 0 - 160. 0 μg/L. The limits of quantification for Chrysoidine G, Basic Yellow 2, Acid Orange I, Acid Orange II and Acid Yellow 36 were 20, 20, 40, 40 and 40 ng/g, respectively. The relative standard deviations of reproducibility of this method for retention time and peak area were no more than 0. 50% and 2. 14%, respectively. The method was applied to determine the recoveries of the above five dyes in chicken; bean products and yellow croaker were between 79. 8% -95. 2% with satisfactory results. Source

Yu S.,Henan University | Yuan J.,Zhengzhou University | Shi J.,Henan University | Ruan X.,Hangzhou Institution of Quality and Technical Supervision and Inspection | And 3 more authors.
Chemometrics and Intelligent Laboratory Systems | Year: 2015

Hologram quantitative structure-activity relationships (HQSAR) and topomer comparative molecular field analysis (CoMFA) were implemented on a group of 73 trans-4-(4-chlorophenyl) pyrrolidine-3-carboxamides of piperazinecyclohexane compounds, and their binding affinities to the melanocortin-4 receptor (MC4R) were determined. The most effective HQSAR model was obtained using atoms and bonds as fragment distinction, and its cross-validated (q2) and non-cross-validated (r2) correlation coefficients were 0.665 and 0.907, respectively. Several external validation parameters (i.e., four ways to calculate the correlation coefficients of the test set samples), namely, QF12, QF22, QF32, and CCC, were 0.781, 0.766, 0.789, and 0.901, respectively. Topomer CoMFA models were built based on three fragment cutting modes. The most effective topomer CoMFA (r2=0.850, q2=0.436, QF12=0.784, QF22=0.779, QF32=0.751, and CCC=0.894) used the amide bond as the cut bond. Meanwhile, the predictive capabilities of the most effective HQSAR and topomer CoMFA models were tested and verified via an external test set with 15 compounds, and then compared with those of previously reported models. Molecular docking was employed to validate the results of the HQSAR and topomer CoMFA models. Moreover, the graphical results of HQSAR, topomer CoMFA, and docking were analyzed and used as guides in designing new MC4R ligands. The results of this work would be useful in chemical library design, virtual screening, and high-throughput screening. © 2015 Elsevier B.V. Source

Discover hidden collaborations