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Liang H.,Hands and Feet Microsurgery | Yu F.,Dalian University | Tong Z.,Hands and Feet Microsurgery | Zeng W.,Hands and Feet Microsurgery
Molecules | Year: 2012

This study investigated the beneficial effect of lycopene on bone biomarkers in ovariectomized (OVX) rats. Female Wistar rats were either sham operated or surgically ovariectomized and then fed with lycopene for 8 weeks. Serum Ca, P, alkaline phosphatase (ALP), interleukin 6 (IL-6) and bone gla protein (BGP) concentration was significantly higher in the untreated OVX group compared with that of the sham group, whereas serum estrogen levels were lower. Bone mineral density (BMD), BMD/wt, bone mineral content (BMC), BMC/wt values, maximum load, stiffness, energy and maximum stress were significantly lower in the untreated OVX group compared with that of the sham group. Administration of lycopene (20, 30 and 40 mg/kg b.w.) for 8 weeks significantly decreased serum Ca, P, ALP, and IL-6 concentration, and enhanced serum estrogen level, BMD, BMD/wt, BMC, BMC/wt values, maximum load, stiffness, energy and maximum stress in lycopene-treated OVX groups. In conclusion, the consumption of lycopene may have the most protective effect on bone in OVX rats. © 2012 by the authors. Source


Liang H.,Hands and Feet Microsurgery | Yu F.,Dalian University | Tong Z.,Hands and Feet Microsurgery | Yuan B.,Hands and Feet Microsurgery | Wang C.,Hands and Feet Microsurgery
Molecular Biology Reports | Year: 2013

This study is designed to investigate the effect of ischemia post-conditioning on IR-induced skeletal muscle injury in limbs of experimental rabbits. Rabbits are randomized to one of the following three groups: sham control, ischemic reperfusion, ischemic postconditioning. The lipid peroxidation level, antioxidant enzymes activities, skeletal muscle mammalian target of rapamycin (mTOR), Bax, Bcl-2 proteins expression and Bcl-2/Bax, and HIF-1α/β-actin mRNA, interleukin-6 (IL-6)/β-actin mRNA and caveolin-3/β-actin mRNA expression were tested in the current study. The results suggested that ischemic postconditioning might decrease lipid peroxidation level, lactic dehydrogenase (LDH), creatine kinase (CK) activities, Bcl-2 proteins expression and Bcl-2/Bax, HIF-1α/β-actin mRNA expression and increase skeletal muscle antioxidant enzymes activities, Bax protein expression and IL-6/β-actin mRNA and caveolin-3/β-actin mRNA expression. These indicated that protective effect of ischemic postconditioning against IR-induced skeletal muscle injury involve into a complex molecular mechanism. Our research results may offer a theoretical guidance for therapy of related clinical diseases. © 2012 Springer Science+Business Media Dordrecht. Source


Liang H.-D.,Hands and Feet Microsurgery | Yu F.,Dalian University | Tong Z.-H.,Hands and Feet Microsurgery | Zhang H.-Q.,Hands and Feet Microsurgery | Liang W.,Hands and Feet Microsurgery
Molecular Biology Reports | Year: 2013

We studied molecular mechanism of Cistanches Herba aqueous extract (CHAE) in ovariectomized (OVX) rats, as an experimental model of postmenopausal osteoporosis. Female rats were either sham-operated or bilaterally OVX; and at 60 days postoperatively. The OVX group (n = 8) received an ovariectomy and treatment with normal saline for 90 days commencing from 20th post ovariectomy day. The ovariectomized +CHAE (OVX + CHAE) group (n = 8) received an ovariectomy and were treated with Cistanches Herba aqueous extract of 100 mg/kg body weight daily for 90 days commencing from 22nd post ovariectomy day. The ovariectomy +CHAE (OVX + CHAE) group (n = 8) received an ovariectomy, and were treated with the of 200 mg/kg body weight daily for 90 days commencing from 20th post ovariectomy day. Serum BGP and TRAP, E2, FSH and LH level, bone marrow Smad1, Smad5, TGF-β1 and TIEG1 mRNA expression levels were examined. Results showed that serum BGP and TRAP, FSH and LH levels were significantly increased, whereas E2, Smad1, Smad5, TGF-β1 and TIEG1 mRNA and proteins expression levels were significantly decreased in OVX rats compared to sham rats. 90 days of CHAE treatment could significantly decrease serum BGP and TRAP, FSH and LH levels, and increase E2, Smad1, Smad5, TGF-β1 and TIEG1 mRNA and proteins expression levels in OVX rats. It can be concluded that CHAE play its protective effect against OVX-induced bone degeneration partly by regulating some bone metabolism related genes, e.g. Smad1, Smad5, TGF-β1 and TIEG1. © 2012 Springer Science+Business Media Dordrecht. Source


Liang H.,Hands and Feet Microsurgery | Yu F.,Dalian University | Tong Z.,Hands and Feet Microsurgery | Sun H.,Hands and Feet Microsurgery | Zhang T.,Hands and Feet Microsurgery
Carbohydrate Polymers | Year: 2012

In this study, ethanol extracts of ginkgo leaf were microencapsulated with maltodextrin, gum arabic or a soluble soybean protein by spray-drying. The results indicated that, for the microcapsules, the encapsulation efficiency of 81.3% was achieved when air inlet temperature was 181°C. The oxidation of ginkgo leaf polyphenol under the conditions was retarded by its microencapsulation with gum arabic, maltodextrin or the soybean protein. Thus, microencapsulation of ethanol extracts of ginkgo leaf significantly improved its oxidative stability. Pharmacological experiment showed that ethanol extracts of ginkgo leaf could enhance ALP activities and collagen I in mouse osteoblast MC3T3-E1 cells. Rabbits pretreated with microcapsules of ethanol extracts of ginkgo leaf significantly inhibited ischemia/reperfusion-induced oxidative injury in rabbits' skeletal muscle. © 2011 Elsevier Ltd. All rights reserved. Source


Haidong L.,Hands and Feet Microsurgery | Fang Y.,Dalian University | Zhihong T.,Hands and Feet Microsurgery | Changle R.,Dalian Municipal Central Hospital
International Journal of Biological Macromolecules | Year: 2011

Microencapsulation of ethanol extract of tea was performed in this study. In order to microencapsulate, β-cyclodextrin was used as wall material. Ethanol extract of tea was used as the core material. Microcapsules in the solid form were obtained by drying the emulsions. RSM showed that optimal processing parameters were as followings: core material/wall material 1/4, β-cyclodextrin content 16%, stirring time 30. min and stirring temperature 200 °C. Pharmacological activities of β-cyclodextrin encapsulation tea extract were determined. It was found that β-cyclodextrin encapsulation tea extract could enhance BMD, BMC and bone Ca, Zn and Cu contents. In addition, β-cyclodextrin encapsulation tea extract could still reduce blood Ca contents. These results indicated that β-cyclodextrin encapsulation tea extract was useful for improving bone quality in aged animals. © 2011 Elsevier B.V. Source

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