Hanabusa Womens Clinic

Kōbe-shi, Japan

Hanabusa Womens Clinic

Kōbe-shi, Japan
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Matsumoto Y.,Hanabusa Womens Clinic | Kokeguchi S.,Hanabusa Womens Clinic | Shiotani M.,Hanabusa Womens Clinic
Reproductive Medicine and Biology | Year: 2017

Aim: To evaluate whether local endometrial injury would improve the pregnancy rates after frozen-thawed blastocyst transfer in cycles with hormone replacement. Methods: Seventy-seven patients who were aged <40 years with a history of at least three unsuccessful embryo transfers and who underwent frozen-thawed blastocyst transfer during hormone replacement cycles between February and June, 2013 were studied. They were allocated into two groups based on the last digit of their clinical record number: an experimental group (n=22), excluding patients who withheld consent or who were unable to undergo treatment, and a control group (n=55). In the experimental group, the endometrium was scratched once with an endometrial sampler during the luteal phase of the cycle preceding the embryo transfer. Results: There was no significant difference in the baseline characteristics between the groups. The clinical pregnancy rate was 6% in the experimental group and 22% in the control group. Among these, the ongoing pregnancy rate was 100% in the experimental group and 50% in the control group. Conclusion: Endometrial injury might increase the pregnancy rates after frozen-thawed blastocyst transfer and decrease the risk of miscarriage in patients with a history of unsuccessful embryo transfers. © 2017 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine.


Otsuki J.,Hanabusa Womens Clinic | Iwasaki T.,Hanabusa Womens Clinic | Tsuji Y.,Hanabusa Womens Clinic | Shiotani M.,Hanabusa Womens Clinic
Journal of Mammalian Ova Research | Year: 2017

Human oocytes have the aggregated chromosome phase (AC phase) during the first and second meiosis. This needs to be better understood, as the timing of ICSI significantly influences ART outcomes. In fact, performing ICSI after the completion of MII spindle formation is known to improve successful fertilization and embryo development. This human AC phase should also be taken into consideration in the application of nuclear transfer/mitochondrial replacement for patients suffering from severe mitochondrial diseases, to prevent the transmission of these diseases to their offspring, with the aim of limiting the risk of mitochondrial carryover. The possible risks and benefits of AC transfer and other procedures for mitochondrial replacement are reviewed and discussed in this paper. © 2017 Japan Society for Ova Research.


Hashimoto H.,Kobe University | Ishikawa T.,Monash University | Goto S.,Hanabusa Womens Clinic | Kokeguchi S.,Hanabusa Womens Clinic | And 2 more authors.
Systems Biology in Reproductive Medicine | Year: 2010

The objective of this study was to explore the relationship between the severity of oligozoospermia and the development of embryos and clinical outcome in patients undergoing ICSI. A total of 908 intracytoplasmic sperm injection cycles involving women of ≤37 years of age were included in this study. The patients were divided into four treatment groups according to the results of an analysis of their husbands' semen: (A) mild oligozoospermia that ranged from 10 × 106/ml to <20 × 106/ml (n=283), (B) mild to severe oligozoospermia that ranged from 5 × 106/ml to <10 × 106/ml (n=192), (C) severe oligospermia that ranged from 1 × 106/ml to <5 × 106/ml (n=259), and (D) very severe oligozoospermia that ranged from 0 to <1×106/ml (n=174). Two pronuclei (PN) oocytes at MII were injected and the development of high quality embryos on day 2, as well blastocyst formation rate on day 5, the implantation rate, clinical pregnancies, and fetal loss, were examined. A lower percentage of two pronuclei (2PN) oocytes in the very severe oligozoospermia group was observed, however, there was no difference in clinical outcome when the oligozoospermic patients were divided by sperm concentration. In addition, no significant difference was detected in zygote production or clinical outcome between spermatozoa with a motility of <40% and spermatozoa with a motility of ≥40%. The results of this study emphasize the importance of selecting good quality sperm for oocyte injection, especially in cases involving very severe oligozoospermia. © Informa Healthcare USA, Inc.


Matsumoto Y.,Hanabusa Womens Clinic | Goto S.,Hanabusa Womens Clinic | Hashimoto H.,Hanabusa Womens Clinic | Kokeguchi S.,Hanabusa Womens Clinic | And 2 more authors.
Fertility and Sterility | Year: 2010

Objective: To report a healthy birth that was achieved by intracytoplasmic sperm injection (ICSI) with use of ejaculated spermatozoa from a patient with Kartagener's syndrome. Design: Case report. Setting: Private infertility clinic. Patient(s): Couple with male factor infertility due to Kartagener's syndrome. Intervention(s): Intracytoplasmic sperm injection with ejaculated sperm. Main Outcome Measure(s): Semen characteristics, sperm motility, fertilization, pregnancy, and birth after ICSI. Result(s): With ejaculated sperm, the fertilization rates were 73% in the first stimulation cycle and 100% in the second cycle. Intracytoplasmic sperm injection was successful. The pregnancy resulted in birth of a single healthy child. Conclusion(s): With ejaculated sperm, successful pregnancy after ICSI in couples with Kartagener's syndrome is possible. Kartagener's syndrome is a heterogeneous group of disorders with similar clinical presentations, and treatment should be individualized depending on sperm motility. © 2010 American Society for Reproductive Medicine.


Furuhashi K.,Hanabusa Womens Clinic | Ishikawa T.,Ishikawa Hospital | Hashimoto H.,Hanabusa Womens Clinic | Yamada S.,Hanabusa Womens Clinic | And 6 more authors.
Andrologia | Year: 2013

An increased risk of testicular cancer in men with infertility and poor semen quality has been reported. In view of the high cure rates for testicular germ cell tumours, increasing clinical importance is being placed on the protection of fertility. High-dose cytostatic therapy may be expected to cause long-term infertility. Thus, the standard procedure for fertility protection is the cryopreservation of ejaculated spermatozoa or testicular tissue before therapy. Four male patients with azoospermia and two patients with very severe oligozoospermia underwent onco-testicular sperm extraction (TESE). We attempted onco-TESE in patients with azoospermia and very severe oligozoospermia after orchiectomy. Of the patients with testicular germ cell tumours, four had spermatozoa in their testicular tissues. Sertoli cell-only syndrome was found in one patient, and one patient showed maturation arrest without the detection of spermatozoa. Three of six showed seminomatous germ cell tumour, two of six had nonseminomatous germ cell tumour and one patient showed no malignancy. Two patients achieved clinical pregnancy. Fertility challenges in men with cancer are the most straightforward because of the relative ease of obtaining and cryopreserving sperm. Testicular sperm extraction is a useful technique for obtaining spermatozoa before cytotoxic therapy in azoospermic and very severely oligozoospermic cancer patients. © 2012 Blackwell Verlag GmbH.


Izumi Y.,Kobe University | Yamaguchi K.,Kobe University | Ishikawa T.,Ishikawa Hospital | Ando M.,Kobe University | And 4 more authors.
Systems Biology in Reproductive Medicine | Year: 2011

Bisphenol-A (BPA) is an industrial chemical and is known to act as an endocrine disrupter. This study was designed to evaluate how BPA regulates Sertoli cell (SC) signal molecules. Purified rat SCs were cultured and treated with BPA (200mol/l) at various time points. Western blot analysis was used to determine the activation of extracellular signal-related kinases 1 and 2 (ERK1/2), c-Jun N-terminal kinase (JNK), p38 mitogen activated protein kinase (MAPK), nuclear factor kappa B (NF-κB), cyclooxygenase-1,2 (COX-1, 2), estrogen receptor-α (ER-α), and androgen receptor (AR). The levels of transferrin (TF), prostaglandin E2 (PGE2), and prostaglandin F2α (PGF2α) in culture medium were quantified by ELISA. Interleukin (IL)-1β and IL-6 mRNAs were measured by quantitative real-time PCR (QRT-PCR). Compared with the control, BPA activated the phosphorylation of ERK1/2 (p-ERK1/2) through 30min to 6h. TF was down-regulated at 6 and 24h. Furthermore, IL-1β was up-regulated at 30min and IL-6 was up-regulated at 1 and 24h. ERK activity inhibitor (PD98059, 10mol/l) inhibited these molecular changes. These results reveal the possibility that BPA may have adverse effects on spermatogenesis via ERK1/2. © 2011 Informa Healthcare USA, Inc.


Otsuki J.,Hanabusa Womens Clinic | Iwasaki T.,Hanabusa Womens Clinic | Katada Y.,Hanabusa Womens Clinic | Sato H.,Hanabusa Womens Clinic | And 4 more authors.
Fertility and Sterility | Year: 2016

Objective To examine the relationship between the inner cell mass (ICM) grade and its morphological configuration on the occurrence of monochorionic diamniotic (M-D) twinning. Design Retrospective embryo cohort study. Setting Private IVF clinic. Patient(s) Evaluation of frozen-thawed single blastocyst transfers with hormone replacement treatment in 8,435. This cohort included 71 blastocysts and their ICMs observed by time-lapse photography. Intervention(s) Any changes in configuration of the ICMs observed by time-lapse photography were analyzed retrospectively. Main Outcome Measure(s) The amount of loosening of blastomeres within the ICM was evaluated by time-lapse observations. The number of cells that were involved in the loosening process was also assessed. Both of these parameters were correlated with the type of monozygotic twinning that eventuated. Result(s) The M-D twinning incidence resulting from blastocysts with a high grade ICM (grade A) were transferred was 0.38% (3/796), whereas it was significantly higher, 1.38% (34/2,463), when blastocysts with a poorer (B and C) grade ICM were transferred. Among 71 transferred frozen-thawed blastocysts that were studied with time-lapse photography, there were two dichorionic diamniotic and one M-D twins. Careful observations of the embryo that resulted in the one M-D case, revealed that the ICM acquired a looser appearance due to decompaction of at least eight cells. This type of decompaction was not observed in the ICMs of other transferred blastocysts. Conclusion(s) The occurrence of M-D twinning may be avoided by excluding blastocysts that contain decompacting ICMs. © 2016 American Society for Reproductive Medicine


Goto S.,Hanabusa Womens Clinic | Kadowaki T.,Shiga University of Medical Science | Tanaka S.,Hanabusa Womens Clinic | Hashimoto H.,Hanabusa Womens Clinic | And 2 more authors.
Fertility and Sterility | Year: 2011

Objective: To present an estimation of the pregnancy rate after IVF or intracytoplasmic sperm injection stratified by blastocyst morphology and age. Design: Retrospective analysis. Setting: Private IVF clinic. Patient(s): A total of 1,488 single frozen-thawed blastocyst transfer cycles. Intervention(s): All frozen-thawed blastocysts used in the study were obtained in the patients' first oocytes retrieval cycles. Main Outcome Measure(s): Clinical pregnancy rate (CPR), viable pregnancy rate (VPR), and delivery rate (DR) according to blastocyst morphological score (Gardner and Schoolcraft score) in three different age groups: women aged 22-33 years, 34-37 years, and 38-45 years. Result(s): The quality of blastocysts degraded as age group increased. The proportions of good-quality blastocysts (grades 4 and above) were 62.3%, 56.3%, and 41.1% in age groups of 22-33 years, 34-37 years, and 38-45 years. Within the same blastocyst quality, CPR, VPR, and DR tended to be lower with increased age. Chances of pregnancy were reduced by 60% or more for women 38 years and older with blastocyst morphology of grades 1 and 2. Significant trends were observed for both age and blastocyst morphology groups. Conclusion(s): There was a significant correlation between blastocyst quality and CPR, VPR, and DR in addition to the influence of age on the three rates. The findings may help predict successful pregnancy in single-blastocyst transfer. ©2011 by American Society for Reproductive Medicine.


PubMed | Hanabusa Womens Clinic
Type: Journal Article | Journal: Fertility and sterility | Year: 2016

To examine the relationship between the inner cell mass (ICM) grade and its morphological configuration on the occurrence of monochorionic diamniotic (M-D) twinning.Retrospective embryo cohort study.Private IVF clinic.Evaluation of frozen-thawed single blastocyst transfers with hormone replacement treatment in 8,435. This cohort included 71 blastocysts and their ICMs observed by time-lapse photography.Any changes in configuration of the ICMs observed by time-lapse photography were analyzed retrospectively.The amount of loosening of blastomeres within the ICM was evaluated by time-lapse observations. The number of cells that were involved in the loosening process was also assessed. Both of these parameters were correlated with the type of monozygotic twinning that eventuated.The M-D twinning incidence resulting from blastocysts with a high grade ICM (grade A) were transferred was 0.38% (3/796), whereas it was significantly higher, 1.38% (34/2,463), when blastocysts with a poorer (B and C) grade ICM were transferred. Among 71 transferred frozen-thawed blastocysts that were studied with time-lapse photography, there were two dichorionic diamniotic and one M-D twins. Careful observations of the embryo that resulted in the one M-D case, revealed that the ICM acquired a looser appearance due to decompaction of at least eight cells. This type of decompaction was not observed in the ICMs of other transferred blastocysts.The occurrence of M-D twinning may be avoided by excluding blastocysts that contain decompacting ICMs.


PubMed | Hanabusa Womens Clinic
Type: Journal Article | Journal: Andrologia | Year: 2013

An increased risk of testicular cancer in men with infertility and poor semen quality has been reported. In view of the high cure rates for testicular germ cell tumours, increasing clinical importance is being placed on the protection of fertility. High-dose cytostatic therapy may be expected to cause long-term infertility. Thus, the standard procedure for fertility protection is the cryopreservation of ejaculated spermatozoa or testicular tissue before therapy. Four male patients with azoospermia and two patients with very severe oligozoospermia underwent onco-testicular sperm extraction (TESE). We attempted onco-TESE in patients with azoospermia and very severe oligozoospermia after orchiectomy. Of the patients with testicular germ cell tumours, four had spermatozoa in their testicular tissues. Sertoli cell-only syndrome was found in one patient, and one patient showed maturation arrest without the detection of spermatozoa. Three of six showed seminomatous germ cell tumour, two of six had nonseminomatous germ cell tumour and one patient showed no malignancy. Two patients achieved clinical pregnancy. Fertility challenges in men with cancer are the most straightforward because of the relative ease of obtaining and cryopreserving sperm. Testicular sperm extraction is a useful technique for obtaining spermatozoa before cytotoxic therapy in azoospermic and very severely oligozoospermic cancer patients.

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