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Kōbe-shi, Japan

Izumi Y.,Kobe University | Yamaguchi K.,Kobe University | Ishikawa T.,Ishikawa Hospital | Ando M.,Kobe University | And 4 more authors.
Systems Biology in Reproductive Medicine | Year: 2011

Bisphenol-A (BPA) is an industrial chemical and is known to act as an endocrine disrupter. This study was designed to evaluate how BPA regulates Sertoli cell (SC) signal molecules. Purified rat SCs were cultured and treated with BPA (200mol/l) at various time points. Western blot analysis was used to determine the activation of extracellular signal-related kinases 1 and 2 (ERK1/2), c-Jun N-terminal kinase (JNK), p38 mitogen activated protein kinase (MAPK), nuclear factor kappa B (NF-κB), cyclooxygenase-1,2 (COX-1, 2), estrogen receptor-α (ER-α), and androgen receptor (AR). The levels of transferrin (TF), prostaglandin E2 (PGE2), and prostaglandin F2α (PGF2α) in culture medium were quantified by ELISA. Interleukin (IL)-1β and IL-6 mRNAs were measured by quantitative real-time PCR (QRT-PCR). Compared with the control, BPA activated the phosphorylation of ERK1/2 (p-ERK1/2) through 30min to 6h. TF was down-regulated at 6 and 24h. Furthermore, IL-1β was up-regulated at 30min and IL-6 was up-regulated at 1 and 24h. ERK activity inhibitor (PD98059, 10mol/l) inhibited these molecular changes. These results reveal the possibility that BPA may have adverse effects on spermatogenesis via ERK1/2. © 2011 Informa Healthcare USA, Inc. Source

Hashimoto H.,Kobe University | Ishikawa T.,Monash University | Goto S.,Hanabusa Womens Clinic | Kokeguchi S.,Hanabusa Womens Clinic | And 2 more authors.
Systems Biology in Reproductive Medicine | Year: 2010

The objective of this study was to explore the relationship between the severity of oligozoospermia and the development of embryos and clinical outcome in patients undergoing ICSI. A total of 908 intracytoplasmic sperm injection cycles involving women of ≤37 years of age were included in this study. The patients were divided into four treatment groups according to the results of an analysis of their husbands' semen: (A) mild oligozoospermia that ranged from 10 × 106/ml to <20 × 106/ml (n=283), (B) mild to severe oligozoospermia that ranged from 5 × 106/ml to <10 × 106/ml (n=192), (C) severe oligospermia that ranged from 1 × 106/ml to <5 × 106/ml (n=259), and (D) very severe oligozoospermia that ranged from 0 to <1×106/ml (n=174). Two pronuclei (PN) oocytes at MII were injected and the development of high quality embryos on day 2, as well blastocyst formation rate on day 5, the implantation rate, clinical pregnancies, and fetal loss, were examined. A lower percentage of two pronuclei (2PN) oocytes in the very severe oligozoospermia group was observed, however, there was no difference in clinical outcome when the oligozoospermic patients were divided by sperm concentration. In addition, no significant difference was detected in zygote production or clinical outcome between spermatozoa with a motility of <40% and spermatozoa with a motility of ≥40%. The results of this study emphasize the importance of selecting good quality sperm for oocyte injection, especially in cases involving very severe oligozoospermia. © Informa Healthcare USA, Inc. Source

Furuhashi K.,Hanabusa Womens Clinic | Ishikawa T.,Ishikawa Hospital | Hashimoto H.,Hanabusa Womens Clinic | Yamada S.,Hanabusa Womens Clinic | And 6 more authors.
Andrologia | Year: 2013

An increased risk of testicular cancer in men with infertility and poor semen quality has been reported. In view of the high cure rates for testicular germ cell tumours, increasing clinical importance is being placed on the protection of fertility. High-dose cytostatic therapy may be expected to cause long-term infertility. Thus, the standard procedure for fertility protection is the cryopreservation of ejaculated spermatozoa or testicular tissue before therapy. Four male patients with azoospermia and two patients with very severe oligozoospermia underwent onco-testicular sperm extraction (TESE). We attempted onco-TESE in patients with azoospermia and very severe oligozoospermia after orchiectomy. Of the patients with testicular germ cell tumours, four had spermatozoa in their testicular tissues. Sertoli cell-only syndrome was found in one patient, and one patient showed maturation arrest without the detection of spermatozoa. Three of six showed seminomatous germ cell tumour, two of six had nonseminomatous germ cell tumour and one patient showed no malignancy. Two patients achieved clinical pregnancy. Fertility challenges in men with cancer are the most straightforward because of the relative ease of obtaining and cryopreserving sperm. Testicular sperm extraction is a useful technique for obtaining spermatozoa before cytotoxic therapy in azoospermic and very severely oligozoospermic cancer patients. © 2012 Blackwell Verlag GmbH. Source

Matsumoto Y.,Hanabusa Womens Clinic | Goto S.,Hanabusa Womens Clinic | Hashimoto H.,Hanabusa Womens Clinic | Kokeguchi S.,Hanabusa Womens Clinic | And 2 more authors.
Fertility and Sterility | Year: 2010

Objective: To report a healthy birth that was achieved by intracytoplasmic sperm injection (ICSI) with use of ejaculated spermatozoa from a patient with Kartagener's syndrome. Design: Case report. Setting: Private infertility clinic. Patient(s): Couple with male factor infertility due to Kartagener's syndrome. Intervention(s): Intracytoplasmic sperm injection with ejaculated sperm. Main Outcome Measure(s): Semen characteristics, sperm motility, fertilization, pregnancy, and birth after ICSI. Result(s): With ejaculated sperm, the fertilization rates were 73% in the first stimulation cycle and 100% in the second cycle. Intracytoplasmic sperm injection was successful. The pregnancy resulted in birth of a single healthy child. Conclusion(s): With ejaculated sperm, successful pregnancy after ICSI in couples with Kartagener's syndrome is possible. Kartagener's syndrome is a heterogeneous group of disorders with similar clinical presentations, and treatment should be individualized depending on sperm motility. © 2010 American Society for Reproductive Medicine. Source

Goto S.,Hanabusa Womens Clinic | Kadowaki T.,Shiga University of Medical Science | Tanaka S.,Hanabusa Womens Clinic | Hashimoto H.,Hanabusa Womens Clinic | And 2 more authors.
Fertility and Sterility | Year: 2011

Objective: To present an estimation of the pregnancy rate after IVF or intracytoplasmic sperm injection stratified by blastocyst morphology and age. Design: Retrospective analysis. Setting: Private IVF clinic. Patient(s): A total of 1,488 single frozen-thawed blastocyst transfer cycles. Intervention(s): All frozen-thawed blastocysts used in the study were obtained in the patients' first oocytes retrieval cycles. Main Outcome Measure(s): Clinical pregnancy rate (CPR), viable pregnancy rate (VPR), and delivery rate (DR) according to blastocyst morphological score (Gardner and Schoolcraft score) in three different age groups: women aged 22-33 years, 34-37 years, and 38-45 years. Result(s): The quality of blastocysts degraded as age group increased. The proportions of good-quality blastocysts (grades 4 and above) were 62.3%, 56.3%, and 41.1% in age groups of 22-33 years, 34-37 years, and 38-45 years. Within the same blastocyst quality, CPR, VPR, and DR tended to be lower with increased age. Chances of pregnancy were reduced by 60% or more for women 38 years and older with blastocyst morphology of grades 1 and 2. Significant trends were observed for both age and blastocyst morphology groups. Conclusion(s): There was a significant correlation between blastocyst quality and CPR, VPR, and DR in addition to the influence of age on the three rates. The findings may help predict successful pregnancy in single-blastocyst transfer. ©2011 by American Society for Reproductive Medicine. Source

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