Hamatologie Labor Mannheim

Mannheim, Germany

Hamatologie Labor Mannheim

Mannheim, Germany

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Sack U.,University of Leipzig | Barnett D.,UK NEQAS for Leucocyte Immunophenotyping | Demirel G.Y.,Yeditepe University | Fossat C.,University Hospital Timone | And 6 more authors.
Cytometry Part B - Clinical Cytometry | Year: 2013

ISO 15189 has been introduced to enable any clinical laboratory, irrespective of geographic location, to be accredited against internationally recognized standards and therefore facilitate direct international comparison of laboratories. Together with increasing use of ISO 15189 for standardization and competition purposes, often triggered by demands of patients and clinicians, clinical flow cytometry laboratories are becoming increasingly challenged to introduce compliant quality management systems. Whilst in most countries, ISO 15189 accreditation is not yet compulsory, there is increasing evidence to suggest that the implementation of this standard is growing. As a result, the European Society of Clinical Cell Analysis (ESCCA) has analysed the impact of accreditation in clinical flow cytometry laboratories. It found, through a discussion forum, that staff qualification, adaptation of multicolour antibody panels, and implementation of a comprehensive quality system (including quality assessment) have been identified as major challenges. Copyright © 2013 International Clinical Cytometry Society.


Birle A.,University of Heidelberg | Nebe C.T.,Hamatologie Labor Mannheim | Hill S.,University of Heidelberg | Hartmann K.,Hamatologie Labor Mannheim | And 3 more authors.
PLoS ONE | Year: 2015

Bacterial infections, even without any perinatal risk factors, are common in newborns, especially in preterm neonates. The aim of this study was to evaluate possible impairment of neutrophil chemotaxis in term and preterm neonates compared with adults as well as neonates with different modes of delivery and anaesthesia. We analysed the expression of the adhesion molecule L-Selectin as well as shape change, spontaneous and N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced transmigration of neutrophils in a flow cytometric assay of chemotaxis after spontaneous delivery with Cesarian Section (CS) under spinal anaesthesia (mepivacaine, sufentanil), epidural anaesthesia (ropivacaine or bupivacaine, sufentanil) or general anaesthesia (ketamine, thiopental, succinylcholine). Chemokinesis was higher (p=0.008) in cord blood neutrophils than in the adult ones, whereas those could be more stimulated by fMLP (p=0.02). After vaginal delivery neutrophils showed a higher spontaneous and fMLP-stimulated chemotactic response compared to neonates after CS without labor. Comparing different types of anaesthesia for CS, spinal anaesthesia resulted in less impairment on chemotaxis than general anaesthesia or epidural anaesthesia. The new flow cytometric assay of neutrophil chemotaxis is an appropriate and objective method to analyse functional differences even in very small volumes of blood, essential in neonatology. Term neonates do not show reduced chemotaxis compared to adults. Preterm neonates present with reduced chemotaxis and chemokinesis, confirming the well known deficits in their neutrophil function. The side effects of maternal drugs on the neonatal immune system have to be considered especially when the immune response is already impaired, as in preterm infants. © 2015 Birle et al.


Nebe C.T.,Hamatologie Labor Mannheim | Dorn-Beineke A.,Dr. Horst Schmidt Kliniken GmbH | Braun P.,Praxis Dr. Knechten | Daniel V.,University of Heidelberg | And 5 more authors.
LaboratoriumsMedizin | Year: 2013

The review of the German guidelines for quality control of medical laboratory investigations made it necessary to determine the actual standard of imprecision and correctness of CD4 and CD8 lymphocyte subsets in peripheral blood. In this study, known laboratories performing that test were approached directly or indirectly via the manufacturers of reagents and equipment, in order to report the data they obtained by validation and internal quality control. In addition, the authors asked for reference ranges and results of the external quality assessments. Due to the fact that no reference methods exist, there are different procedures in the field and round-robin definition of subgroups with different target values obtained by consensus. This article describes the different procedures and technologies as well as the given spread of results. The report also covers the remaining lymphocyte subsets to gain a better understanding of the context and background of the technology.


PubMed | Hamatologie Labor Mannheim, Medical School Hamburg and University of Heidelberg
Type: Journal Article | Journal: PloS one | Year: 2015

Bacterial infections, even without any perinatal risk factors, are common in newborns, especially in preterm neonates. The aim of this study was to evaluate possible impairment of neutrophil chemotaxis in term and preterm neonates compared with adults as well as neonates with different modes of delivery and anaesthesia. We analysed the expression of the adhesion molecule L-Selectin as well as shape change, spontaneous and N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced transmigration of neutrophils in a flow cytometric assay of chemotaxis after spontaneous delivery with Cesarian Section (CS) under spinal anaesthesia (mepivacaine, sufentanil), epidural anaesthesia (ropivacaine or bupivacaine, sufentanil) or general anaesthesia (ketamine, thiopental, succinylcholine). Chemokinesis was higher (p=0.008) in cord blood neutrophils than in the adult ones, whereas those could be more stimulated by fMLP (p=0.02). After vaginal delivery neutrophils showed a higher spontaneous and fMLP-stimulated chemotactic response compared to neonates after CS without labor. Comparing different types of anaesthesia for CS, spinal anaesthesia resulted in less impairment on chemotaxis than general anaesthesia or epidural anaesthesia. The new flow cytometric assay of neutrophil chemotaxis is an appropriate and objective method to analyse functional differences even in very small volumes of blood, essential in neonatology. Term neonates do not show reduced chemotaxis compared to adults. Preterm neonates present with reduced chemotaxis and chemokinesis, confirming the well known deficits in their neutrophil function. The side effects of maternal drugs on the neonatal immune system have to be considered especially when the immune response is already impaired, as in preterm infants.


Nebe T.,Hamatologie Labor Mannheim | Stamminger G.,Zentrum fur Diagnostik GmbH Am Klinikum Chemnitz | Baum H.,Regionale Kliniken Holding Neckar Schwarzwald GmbH | Ossendorf M.,MVZ Labor Limbach | Wittmann G.,Abteilung F. Transfusionsmedizin
LaboratoriumsMedizin | Year: 2014

Kryoproteine werden immer noch in hohem Maße unterdiagnostiziert, obwohl sie von besonderer klinischer Relevanz sind. Kryoglobuline, Kryofibrinogen bzw. Kälteantikörper haben eine deutliche Assoziation zu Autoimmunerkrankungen bzw. Non-Hodgkin-Lymphomen. Die Bestimmung ist einfach, aber die präanalytischen Voraussetzungen sind eine Herausforderung. Der Artikel fasst die wesentlichen Aspekte kurz zusammen um diese Situation zu verbessern. © 2014 by De Gruyter 2014.


Nebe T.,Hamatologie Labor Mannheim
LaboratoriumsMedizin | Year: 2014

Die hämatologische Labordiagnostik von Thrombozyten weist eine Reihe von technischen Schwierigkeiten auf und stellt besondere Anforderungen an die Präanalytik. Auch der Umgang mit Aggregationsphänomenen wirft in der täglichen Routine immer wieder Fragen auf. Der Artikel fasst den gegenwärtigen Stand der Thrombozytenzählung zusammen und der notwendigen Differentialdiagnostik. © 2014 by De Gruyter 2014.


Nebe C.T.,Hamatologie Labor Mannheim
LaboratoriumsMedizin | Year: 2015

Hematological laboratory diagnostics of platelets is faced with technical difficulties and requires preanalytical considerations. The handling of platelet agglutination needs to be addressed in the daily routine. This article summarizes the current state of platelet counting and subsequent differential diagnosis.

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